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Citations for Takara Bio :
51 - 100 of 805 citations for Chromosome 10 Open Reading Frame 82 C10ORF82 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... overhangs (see table for primer sequences) and fused in frame at its carboxyl end via GA to eGFP in the vector eGFP-N3 (CLONTECH), to generate flZEB1-GFP ...
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bioRxiv - Genetics 2021Quote: ... fust-1 genomic fragment containing sequences from 2181bp upstream ATG to just before stop codon was cloned into the Sma I site of pHK-mRFP vector in frame with mRFP by In-Fusion HD Cloning Kit (Takara). This plasmid was further used to generate backbone structure containing fust-1 promoter and mRFP ...
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bioRxiv - Molecular Biology 2021Quote: ... SmRho1.1-pCR4-TOPO and SmRho1.2-pCR4-TOPO were cut by BamHI and NdeI and the sequences were inserted in frame into the pGADT7-AD expression vector (Clontech) using the T4 DNA Ligase (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... The CWLP coding region without signal peptide (SP) was cloned in frame downstream of the GAL4 DNA-binding domain in the bait vector pGBKT7 (Clontech) and introduced into the yeast strain AH109 (Clontech) ...
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bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and EcoRV sites of pcDNA-EGFP-P2A in frame with the tag sequence by using an In-Fusion® HD Cloning Kit according to the manufacturer’s instructions (Takara). pcDNA-hAPOBEC1 was constructed by cloning the XhoI-HindIII fragments of pUC57-APOBEC1 ...
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bioRxiv - Biophysics 2023Quote: ... A plasmid expressing mouse GR tagged in the N-terminus with mCherry was developed by amplifying mouse GR coding sequence and in-frame cloning in pmCherry-C3 (Clontech). Point mutations and deletions were introduced using the Quickchange XL mutagenesis kit (Agilent ...
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bioRxiv - Developmental Biology 2023Quote: ... G9 deletion mutants were generated by PCR amplification or overlap extension PCR and inserted in frame with the MYC tag into the pCS2-MT-NLS expressing vector using the In Fusion Protocol (ST0345, Takara). All constructs were confirmed by Sanger sequencing.
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bioRxiv - Cell Biology 2024Quote: ... where the entire ORF of the corresponding gene was inserted in frame into the pGADT7 (GAL4 activation domain) or pGBKT7 (GAL4 binding domain) plasmid (Clontech).
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bioRxiv - Microbiology 2023Quote: ... Amplified DNA encoding the sequence for H2A was ligated in frame with the BglII and SalI restriction digest sites of pEGFP-C1 (Clontech). To construct the pEGFP-H2A.X expression plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... Full-length WDR5 was cloned in frame as N- or C-terminal NanoLuc-fusion pNLF1 vector using ligation independent in-fusion cloning (Takara Bio) and sequence verified ...
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Lateral organ diversification in plants mediated by the ALOG protein family of transcription factorsbioRxiv - Plant Biology 2019Quote: ... A PCR-amplified eGFP coding sequence was inserted in frame with the 5’ end of the MpTAW1 coding sequence by the In-Fusion cloning reaction (TaKaRa, Japan) to generate the proMpTAW1:eGFP-MpTAW1 plasmid ...
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bioRxiv - Molecular Biology 2022Quote: The G3BP1 cDNA sequence was amplified from a HeLa-JVM total cDNA library and concurrently inserted in-frame with a mCherry-coding sequence into a lentiviral vector (pCW)110 using the in-Fusion Cloning Kit (TaKaRa Bio). The HERC5 ...
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bioRxiv - Developmental Biology 2023Quote: ... and cloned it (in frame) into the linearized LV-GFP plasmid using In-Fusion® Snap Assembly Master Mix (Takara #638947). High titer viruses were generated as previously described 56 ...
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bioRxiv - Genetics 2023Quote: ... The 3’ MYH7-eGFP and SV40pA fragments were isolated from a full-length MYH7 cDNA cloned in-frame into pEGFP-N1 (Clontech Laboratories) by PCR with a SalI site at 3’-end ...
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bioRxiv - Microbiology 2020Quote: ... and was inserted into pMSR in-frame with mycobacterial codon-optimized Dendra2 using In-Fusion cloning (Takara Bio, Mountain View, CA USA). This construct was transformed by electroporation into M ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1%BSA and 10% FBS.Cells were incubated with anti-c-Myc antibody (Clontech Laboratories, USA) at a 1:10 dilution for 16 hr at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: PCR products were subcloned in frame with GFP sequence in 3′ into pCS2+-GFP vector using In-Fusion® HD Cloning Kit (Takara Bio USA, Inc.). For rescue experiments ...
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bioRxiv - Neuroscience 2022Quote: cDNA fragments corresponding to Inka1 and Inka2 ORFs were isolated using RT-PCR of the RNAs of E12 embryonic and adult mice brains and subcloned in-frame into a pEGFP-C2 expression vector (Takara Bio Inc., Shiga, Japan). To construct the CAG promoter-driven Inka2 in mammalian cells ...
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bioRxiv - Genomics 2019Quote: ... 10 µl of 10 mM dNTPs (Clontech #639125), 2.5 µl RNase Inhibitor (Lucigen) ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol) containing DNase I (10 U, Takara) and incubated at 37 °C for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min before being resolved by SDS-PAGE and subjected to western blotting using anti-6xhis antibodies (TaKaRa: 631212) as primary antibody and HRP conjugated anti-rabbit (Sigma Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... 10% FBS (Clontech), 50 U/mL penicillin ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibody rabbit anti-human Cas9 Polyclonal Antibody (Clontech) was diluted 1 ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
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bioRxiv - Molecular Biology 2021Quote: ... with 10% FBS (TaKaRa, 631106). HCT116 OsTIR1 cells (kindly provided by Masato Kanemaki ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (Clontech), 100 units/mL penicillin ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 10 % FBS (Clontech), penicillin ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μL PCR mixture (TaKaRa), 1 μL upstream primer (10 μmol/L) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% FBS (Clontech) in a humidified atmosphere of 5% CO2 at 37°C.
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bioRxiv - Systems Biology 2021Quote: ... supplemented with 10% FBS (Clontech), GlutaMAX supplement (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% FBS (Clontech), 100 units/mL penicillin ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 10% FBS (Takara), 1% NEAA (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Clontech) and 2 mM L-glutamine (PAN Biotech ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...
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bioRxiv - Genomics 2020Quote: ... 10 μL of Mixture (TaKaRa, Japan), 1 μL upstream primers (10 μmol/L ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of Clone AMP (Takara), and 300 nM each of primers ks1 and mf83 ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 U μl–1 SMARTScribe (Clontech) and 10 U μl–1 RNASin plus (Promega) ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... 10% Tet system approved FBS (Takara), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 10% Fetal Bovine Serum (Clontech). Cells were grown at 37°C with 5% CO2 and passaged using 0.05% Trypsin-EDTA solution (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10% Tet Approved FBS (Clontech Laboratories), and 1X Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... 10-15 µg of EGFP (Clontech) was coated onto 6-8 mg of 1.6 µm gold beads.
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bioRxiv - Plant Biology 2022Quote: ... using 10 pmol random nonamers (Takara) in 20 μl reaction volume and according to manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... with 10% FBS (Takara Bio, 631368) and 1x penicillin-streptomycin in 24-well glass-bottomed No ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 U recombinant inhibitor (TaKaRa) for samples containing biological inhibitor ...
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bioRxiv - Synthetic Biology 2024Quote: ... supplemented with 10% FBS (Takara 632180). LentiX cells (Takara 632180 ...
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bioRxiv - Molecular Biology 2021Quote: ... DMEM supplemented with 10% fetal bovine serum (FBS) and 10 μl DNase I (Takara Bio, Shiga, Japan) was then added to the samples and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used were GAL4 AD Mouse Monoclonal Antibody (Takara Bio USA, Inc. #630402), GAL4 DNA-BD Mouse Monoclonal Antibody (Takara Bio USA ...
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bioRxiv - Neuroscience 2020Quote: ... and incubation with the primary antibody (polyclonal rabbit anti-dsRed antibody, 632496, Clontech, dilution 1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... SMAD2 #3122 and SMAD3 #9523 antibodies (Cell Signalling Technology) and anti-FLAG antibody (Clontech). Horseradish peroxidase-conjugated secondary antibodies and anti-rabbit and anti-mouse antibodies were acquired from Molecular Probes ...
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bioRxiv - Synthetic Biology 2021Quote: ... The following antibodies were used: Anti-GFP antibody (JL-8 from Takara, Shiga, Japan), anti-Flag antibody (M2 from Sigma-Aldrich ...