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Citations for Takara Bio :
1 - 50 of 805 citations for Chromosome 10 Open Reading Frame 82 C10ORF82 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... open reading frames were PCR-amplified using CloneAmp HiFi PCR Premix (Clontech), gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... The TOP1MT open reading frame (ORF) was cloned using InFusion cloning (Takara, Clonetech) into the pRV100G retroviral vector containing a C-terminal T2A mCherry tag ...
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bioRxiv - Cell Biology 2021Quote: ... the full-length Borr open reading frame was amplified from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... and L gene open reading frames (ORF) were amplified with PrimeSTAR® Max DNA Polymerase (TAKARA) and ligated between the NcoI and BamHI sites of the pTM1 vector with In-Fusion® Snap Assembly Master Mix (TAKARA ...
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bioRxiv - Biochemistry 2020Quote: ... The DHTKD1 open reading frame (amino acids 25-919) was amplified using PrimeSTAR GXL DNA Polymerase (Takara) and primers forward (5’-GGT TTA GAA TTC ATG CAG ACC GAG CGG GGC GTT TA-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... the JAK3 open reading frame was amplified by PCR using the Advantage 2 polymerase mix (Takara Bio) with JAK3-ORF primers (Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid pIRESpuro3-EGFP was constructed by excising the EGFP open reading frame from pEGFP-C1 vector (Clontech) with NheI and BamHI digestion and insertion into the same restriction sites of the pIRESpuro3 vector (Clontech ...
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bioRxiv - Cell Biology 2021Quote: ... the open reading frame was amplified from a larval brain cDNA library using CloneAmp HiFi PCR Premix (Takara Bio). For the HA-tagged version ...
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bioRxiv - Cancer Biology 2022Quote: ... The HAND2 mutant (HAND2mut) open reading frame was cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD (Takara Bio ...
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bioRxiv - Developmental Biology 2021Quote: All novel plasmids constructed for this study were based on the pSP Sox1/2/3> plasmid previously described (9) with the open reading frames replaced by PCR amplifications using a proofreading DNA polymerase (Primestar, Takara) and plasmids were assembled from linear PCR products using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pJYB240 was generated from pJYB212 by exchanging the mEos2 open reading frame by a PCR-amplified mTurquoise2 DNA (Goedhart et al. 2012) using the InFusion kit (Clontech). The parBF-mTurquoise2 gene from pJYB240 was introduced into pJYB243 ...
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bioRxiv - Physiology 2019Quote: ... a tetracycline-regulated Kcnn4 expression construct was generated by subcloning PCR-amplified cDNA encoding the open reading frame of murine Kcnn4 (gene ID16534)) into the pTRE-Tight expression vector (Clontech). The construct was verified by sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... the open reading frame of the OBE3 gene was cloned into XmaI/BamHI sites of the pGADT7 plasmid (Clontech, Palo Alto) resulting into pEW11.
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bioRxiv - Plant Biology 2021Quote: ... For expressing the GAL4BD-SMXL5 fusion protein in yeast the open reading frame of the SMXL5 gene was cloned into XmaI/BamHI sites of the pGBKT7 plasmid (Clontech, Palo Alto) resulting in pEW6 ...
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bioRxiv - Developmental Biology 2024Quote: The open reading frame (ORF) of Kaede was removed from pSPCiPC2Kaede by inverse PCR with PrimeStar GxL DNA polymerase (Takara-bio Inc). The ORFs of Gαq ...
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bioRxiv - Cell Biology 2020Quote: ... The four ALX1 exons encoding the open reading frame were amplified using the CloneAmp HiFi PCR premix (Takara Bio Inc., Kusatsu, Shiga, Japan) and exon specific ALX1 oligonucleotides ...
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bioRxiv - Microbiology 2022Quote: ... The open reading frame encoding each protein was amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa Bio, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Microbiology 2023Quote: The open reading frames of 19 LD-associated proteins were amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... Full-length β-catenin and a C-terminal deletion of tle3b (NM_131780, complete reading frame after amino acid 210) were cloned in pGAD (Clontech). Combinations of plasmids to test two-hybrid interactions were co-transformed in Y2Gold yeast strain (Suppl ...
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bioRxiv - Cancer Biology 2019Quote: ... The open reading frame of AIRE was recovered by PCR using this plasmid as a template and PrimeSTAR GXL DNA Polymerase (Takara, Shiga, Japan). The PCR product was digested with EcoR1 and inserted into pcDNA6-3xFlagN (in-house plasmid made using pcDNA6/V5-HisA (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... proof-reading PrimeStar HS DNA Polymerase (Takara) was used following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... RNC 82-aa C34A with [SG]5 or [SG]10 repeats were constructed by the Prime STAR MAX (Takara Bio Inc., Japan) method using appropriate primers (Table 1).
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bioRxiv - Immunology 2022Quote: ... was amplified using a high-proof reading polymerase (CloneAmp HiFi, Takara) with primers containing EcoRI and NotI restriction sites and subcloned into pCR blunt II TOPO vector for subsequent restriction and ligation into the pcDNA3 expression vector ...
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bioRxiv - Cell Biology 2022Quote: ... followed by in-frame cloning into eucaryotic expression vectors pEGFP-C1 (Clontech) or a custom-built Flag-C1 vector ...
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bioRxiv - Biochemistry 2020Quote: ... Human IFITM3 cDNA was purchased from Open Biosystems and PCR cloned into pCMV-HA vector (Clontech). All mutants of IFITM3 were generated by using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Biochemistry 2019Quote: ... CoAA and hnRNP M fragments were inserted in-frame into pM vector (Clontech) containing Gal4 DNA-binding domain to produce Gal4-fusion proteins ...
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bioRxiv - Microbiology 2022Quote: ... US10 was cloned in frame with eGFP in the pCMS-eGFP vector (Clontech) using primers shown in Table 3 ...
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bioRxiv - Microbiology 2019Quote: All PCR for assembling transforming DNA were obtained by proof-reading polymerase (PrimeStar Max, Takara). Genomic DNA from Legionella was isolated using the Wizard Genomic DNA Purification Kit or the Maxwell DNA extraction kit (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... were subcloned in frame from their corresponding SMAUG1-HisTEV2xFLAG plasmids into pEGFP-N1 (Clontech) using XhoI and BamHI ...
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bioRxiv - Biochemistry 2020Quote: ... The targeted gene and its flanking sequences were amplified by PCR with Synechocystis chromosome DNA as the template and cloned into pMD18–T vector (Catalog No: D101A, Takara, Japan). The physical map of the Synechocystis genome region containing sll0445–sll0447 or sll1334 ...
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bioRxiv - Molecular Biology 2021Quote: The sequence encoding SmHDAC8 was inserted in frame into the pGBKT7-BDB expression vector (Clontech) using the T4 DNA Ligase (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... the pGADT7-Sfi I three-frame primary cDNA library was constructed by Takara (Dalian, China) using leaf samples from N9134 after infected with stripe rust fungi at 24 and 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... Amplified DNA encoding the sequence for H2A.X was ligated in frame with pEGFP-C1 (Clontech) at the BglII and SalI restriction sites.
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bioRxiv - Bioengineering 2022Quote: ... and TP–mAG were ligated into the open circular pET-29b(+) using the In-Fusion HD Cloning Kit (TaKaRa) to generate the recombinant protein expression vectors ...
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bioRxiv - Genetics 2020Quote: ... Human CAD was PCR amplified from cDNA (Open Biosystems clone ID 5551082) using specific primers (Table S1) and ligated with In-Fusion (Clontech) into NotI linearized pcDNA3.1-GFP ...
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bioRxiv - Immunology 2019Quote: ... Rab 5 and Rab 7 cDNA (a gift of M. Sandor) and human CD81 cDNA (Open Biosystems) were cloned into pmCherry-N1 vector (Clontech). Raji/DC-SIGN cells were electroporated with 1 µg of indicated plasmid using the Eppendorf Multiporator in iso-osmolar electroporation buffer using a 90 µs ...
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bioRxiv - Cell Biology 2019Quote: ... TRPV4(ΔPR)–myc and TRPV4(ΔAR1-3)–myc were constructed using PCR with template plasmids from Open Biosystems subcloned into pCMV-myc vector (Clontech) at the SalI/XhoI sites ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Full-length Dnmt1 cDNA clone was obtained from Open Biosystems and further subcloned into the pLVX lentiviral expression vector (Clontech) as we previously described (Wang et al. ...
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bioRxiv - Microbiology 2023Quote: The FLAG-vPOL construct was generated by amplifying the MHV68 vPOL from the recombinant MHV68 M3-Luc bacterial artificial chromosome (BAC) (39) with PCR followed by insertion in the p3xFLAG-Myc-CMV-24 vector (CloneTech Takara Biotech, Mountainview, CA) at the EcoRI and SalI restriction sites ...
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bioRxiv - Cell Biology 2019Quote: ... followed by in-frame cloning into XhoI-ApaI sites of pEYFP-N1 (Clontech, Palo Alto, CA, USA). RNF167 WT and all the variants were cloned into pMYs-IRES-Puro (Cell Biolabs ...
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bioRxiv - Genetics 2020Quote: ... in frame with Myc and Flag tag by In-Fusion HD EcoDry Cloning Plus System (Takara Bio). In order to insert a poly histidine tag for protein expression and purification ...
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bioRxiv - Microbiology 2023Quote: ... These PCR products were digested by SalI and NotI and subcloned in frame into pCMV2-HA (Clontech) cut by the same enzymes ...
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bioRxiv - Microbiology 2023Quote: ... H2AB2) was ligated in frame with the BglII and PstI restriction digest sites of pEGFP-C1 (Clontech). To construct the pEGFP-macroH2A1.2 expression plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... products were cut with KpnI/AgeI and cloned in frame into the same sites of pEGFP-N1 (Clontech). The Gαo-Nt8–31-GFP sequence was PCR-amplified from Gαo-Nt31-GFP ...
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bioRxiv - Microbiology 2023Quote: ... The macroH2A gene was ligated in frame with the BglII and PstI restriction sites of pEGFP-C1 (Clontech).
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bioRxiv - Biochemistry 2021Quote: ... falciparum cDNA followed by Ligation Independent Cloning into HindIII/KpnI-cleaved plasmid pOPIN F (82) using the In-Fusion HD EcoDry Cloning Kit (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... falciparum cDNA followed by Ligation Independent Cloning into HindIII/KpnI-cleaved plasmid pOPIN F (82) using the In-Fusion HD EcoDry Cloning Kit (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The human HSF2-YFP was constructed by PCR and cloned into the XhoI and SalI sites in frame with the N-terminal YFP tag in EGFp-C1 plasmid using In-Fusion Kit (Clontech). All PCR-amplified products for both plasmids were sequenced to exclude the possibility of second site mutagenesis ...
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bioRxiv - Developmental Biology 2019Quote: ... plasmid after digestion of the inserts by EcoRI and KpnI and cloning into the EcoRI and EcoRV sites in frame with the C-terminal Flag tag in pSNAPf plasmid using In-Fusion Kit (Clontech). The human HSF2-YFP was constructed by PCR and cloned into the XhoI and SalI sites in frame with the N-terminal YFP tag in EGFp-C1 plasmid using In-Fusion Kit (Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... University of York) in frame with an N-terminal His tag and Im9 solubility tag (37) using In-Fusion cloning (Takara). Primers used for gene amplification were 5’-TCCAGGGACCAGCAATGCTTTCTGAGGAAGAGCAAAAAC-3’ and 5’-TGAGGAGAAGGCGCGTTAAAAGCGATAGCGGTAGCGGATG-3’ for UBA1a ...