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Citations for Takara Bio :
51 - 100 of 136 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The standard curve was prepared using the positive control solution provided by Takara with serial dilution for absolute quantification ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinked membranes were prehybridized for ∼1 hour at 55C in hybridization solution (Takara). DNA oligos (Table S1 ...
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bioRxiv - Bioengineering 2024Quote: ... Eluted solution was then loaded on TALON resin (Takara Bio Inc., Kusatsu, Japan) (bead volume ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were analyzed on bleach agarose gels (0.06% bleach, 1% (w/v) agarose) for visualization of the RNA and on WB (anti-His antibody, Clontech) for visualization of His6-tagged Nsp1.
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bioRxiv - Plant Biology 2023Quote: ... The gRNA was then ligated into BsaI linearized pKSE401 using DNA ligation Kit v.2.0 (TaKaRa, Kusatsu, Shiga, Japan).
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bioRxiv - Microbiology 2021Quote: ... and hybridisation was performed overnight at 40 °C in ExpressHyb Hybridisation Solution (Clontech, USA). Membranes were then rinsed and washed at 40 °C 4 x 15 min with a wash buffer containing 1% sodium dodecyl sulphate [SDS] and 1x saline-sodium citrate (SSC) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Microbiology 2023Quote: ... The solution was mixed with DNA Ligation Kit
(6023, Takara Bio Inc.) and used for transformation with NEB 5-alpha F′Iq Competent E ... -
bioRxiv - Genomics 2023Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Neuroscience 2023Quote: ... and then the virus solution was concentrated using the Lenti-X Concentrator (631231, Clontech) and suspended in PBS (in vivo conditions).
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bioRxiv - Genetics 2023Quote: ... The viral solution was further concentrated using the Lenti-X™Concentrator (Takara: 631231) according to the manufacturer’s instructions and stored at -80°C for long-term use ...
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bioRxiv - Molecular Biology 2024Quote: ... Hybridization was performed according to the manufacturer’s manual using ExpressHyb Hybridization Solution (Takara Bio) and the radioactive signal was detected using a Phosphorimager system ...
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bioRxiv - Developmental Biology 2021Quote: ... The libraries for RNA sequencing were generated using SMART-Seq v.4 Ultra Low Input RNA (Takara Bio, cat. #634890) and Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... were added to the lysates of single parasites and libraries were prepared using SMART-Seq v.4 Ultra Low Input RNA Kit (Takara) using a quarter of the reagent volumes recommended by the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... Transferred membranes were blocked with 5% (w/v) milk for 30 mins at RT before being hybridized with the corresponding anti-GFP (632381, Takara) or anti-mCherry (632543 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... The sequencing reads were aligned to the mm10 genome using the Cogent NGS Analysis Pipeline (CogentAP, Takara Bio, v.1.5.1). Gene-barcode count matrices were then analyzed using R (v.4.0.0 ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3) in ExpressHyb solution (Clontech) and scanned after exposition with Typhoon FLA 9500 scanner (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... wells were first coated with 100μl of RetroNectin solution (32μg/ml in PBS, Takara Bio), incubated for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted solution was concentrated using an Amicon Ultra-15 centrifugal filter unit (Takara bio). The titre was measured via real-time PCR using an AAVpro Titration Kit for Real Time PCR Ver ...
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bioRxiv - Immunology 2024Quote: ... The reaction solution was prepared according to the instructions of the reverse transcription kit (Takara) to reverse-transcribe RNA to cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... followed by blocking solution containing primary antibodies against mCherry (mouse monoclonal 1:500, Takara Bio) and c-Fos (rabbit polyclonal 1:500 ...
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Exploratory mass cytometry analysis reveals immunophenotypes of cancer treatment-related pneumonitisbioRxiv - Immunology 2024Quote: ... The resultant cell pellets were then resuspended in Cellbanker 1 cryopreservation solution (Takara, catalog #210409). This suspension was aliquoted into cryovials and gradually frozen to –80°C and stored at –80°C until required for experimental analysis ...
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bioRxiv - Genomics 2024Quote: ... The viral solution was further concentrated using the Lenti-X™ Concentrator (Takara, catalog #631231) according to the manufacturer’s instructions followed by snap freezing in liquid nitrogen and storage at -80 °C for long-term use.
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Cell Biology 2024Quote: Arrayed slides were blocked in PBST containing 3% (w/v) powdered milk within an Atlas Glass Hybridisation Chamber (Clontech, CA, USA) then hybridised to 400µl fluorophore-tagged peptide for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Hybridization of the membrane with the probe was performed using the ExpressHyb™ Hybridization solution (TaKaRa).
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bioRxiv - Plant Biology 2022Quote: ... protein solution was collected and subjected to purification using His60 Ni Superflow resin (TaKaRa, California, USA) to remove 6×His-SUMO tag from the protein preparations ...
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bioRxiv - Plant Biology 2021Quote: ... according to the manufacturer’s instructions and in combination with Fruit-mate for RNA Purification solution (Takara Bio Europe SAS ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA solution was subjected to RT-qPCR using TB Green Premix Ex Taq (Takara, rr420a) and prime script rt master mix (Takara ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA extraction with TRIZOL reagent and a high salt solution for precipitation (plant) (Takara Bio, Japan) was conducted according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.8 μl reaction stop solution containing 5.3% SDS and 6.6 mg/mL proteinase K (9034, Takara) was added and the sample was incubated for 60 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... The solution was replaced with Laminin 511 E8 fragment (Imatrix511, Catalog #T304, TAKARA BIO, Kusatsu, Japan) solution (30 µL in 1 mL of PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... each pipette was filled with 3μl of pipette solution which consisted of 5% RNase inhibitor (Takara) in RNase-free PBS (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The solution was treated with 5 μg/mL RNaseA and 70 unit/ml DNase I (Takara) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting solution was applied to 0.5 mL of TALON Metal Affinity Resin (#635501; Takara Bio), washed with Equilibration/Wash buffer ...
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Genomics 2020Quote: ... cells were loaded onto a source plate and dispensed in to a SMARTer ICELL8 350 v chip (Takara Bio USA, Cat. # 640019) at 35 nanoliter per well ...
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bioRxiv - Microbiology 2022Quote: ... The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA). The EMSA binding reaction ...
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bioRxiv - Cell Biology 2024Quote: ... Constructs that express wrmScarlet-tagged V-ATPase components with gfp::unc-54 3’ UTR were generated using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). The primers were listed in Extended Data Table 3.
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bioRxiv - Immunology 2020Quote: Levels of p24 antigen in the purified SARS-CoV-2 pseudotype solution was measured by ELISA (Takara). Mouse sera were heat inactivated ...
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bioRxiv - Synthetic Biology 2022Quote: ... After removal of the blocking solution 100 μL of anti-GFP (Living Colors GFP monoclonal antibody, Clontech) diluted 1:10,000 in antibody solution (1x PBS ...
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bioRxiv - Biochemistry 2024Quote: ... AAV virus was extracted from the cells using the AAVpro® Extraction Solution kit (Catalog# 6235, Takara). Fourteen-month-old mice (n=30 ...
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bioRxiv - Developmental Biology 2021Quote: Full length human COG4 was cloned into pCS2+ vector from plasmid hCOG4-siR-3myc in AAZ6 (Gift from Professor Vladimir V. Lupashin) using In-Fusion® HD Cloning Kit (TaKaRa Bio, 638909) with primers 5’-ATGGGAACCAAGATGGCGGA-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified RNA served as input for the cDNA library preparation with SMART-Seq v.4 Ultra Low Input RNA kit (Takara Bio, Kusatsu, Japan) and Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... Ten nanograms of RNA were used for preparing indexed libraries using SMARTer Stranded Total RNA-Seq Pico-Input Mammalian kit v.3 (Takara Bio. Cat# SKU: 634487) as per manufacturer’s instructions (except that fragmentation was performed for 3 minutes of fragmentation at 94 °C and 13 cycles was used for PCR2) ...
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bioRxiv - Cell Biology 2024Quote: ... Ten nanograms of RNA were used for preparing indexed libraries using SMARTer Stranded Total RNA-Seq Pico-Input Mammalian kit v.3 (Takara Bio. Cat# SKU: 634487) using manufacturer’s instructions with a couple of modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... pooled and filtered through a 0.45μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech). Freshly concentrated supernatants were added directly to drained sub-confluent recipient cells and incubated overnight ...
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bioRxiv - Neuroscience 2020Quote: ... we combined 494 μL of internal solution with 6 μL of recombinant RNase inhibitor (1 U/μL, Takara) in order to increase RNA yield ...