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Citations for Takara Bio :
1 - 50 of 136 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Protoplast were stained following manufactured instructions from ApoAlertTM annexin V-FITC kit (Takara) in modified annexin binding buffer containing 1.2M sorbitol ...
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bioRxiv - Microbiology 2020Quote: Phosphatidylserine (PS) externalization was evaluated by staining protoplasted cells with Annexin V-FITC (Takara). Protoplast were generated as previously described (74) ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 10% (v/v) Tet-system approved FBS (Clontech), 100 U/mL penicillin ...
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bioRxiv - Immunology 2024Quote: ... TALON metal affinity resin (Takara, #635653, 0.5-1:1 v/v ratio) was washed with PBS and added to the supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... 1/3 (v/v) of LentiX™ Concentrator reagent (Clontech, Mountain View, USA) was added and incubated overnight (o/n) ...
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bioRxiv - Biophysics 2024Quote: ... the supernatant was incubated with 1 ml 50% v/v Talon resin (Clontech) and 5 mM imidazole pH 8.0 per initial litre of cell culture for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... and sgRNA were cultured at 37°C and 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, HyClone) supplemented with 10% (v/v) FBS (ClonTech), 250 µg/ml hygromycin ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 1 h at room temperature with Odyssey blocking buffer and 0.2 % PBS-T (PBS containing 0.2 % (v/v) Tween-20) with rat anti-HA clone 3F primary antibody (Clontech) at 1:1,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl/well of beads were washed twice with TE solution and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). Then ...
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bioRxiv - Biophysics 2021Quote: ... Cells were seeded at 25-30% confluency onto the prepared coverslips placed in 6-well plates in cell imaging media comprising phenol-red free DMEM (HyClone) supplemented with 10% (v/v) FBS (ClonTech) and 1 mM sodium pyruvate and allowed to settle for at least 4h ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Microbiology 2021Quote: ... in solution and stopped by adding guanidinium thiocyanate solution buffer NTI (Takara Bio). After the methylation reaction following DNA purification ...
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bioRxiv - Cell Biology 2020Quote: ... foetal bovine serum or 10% (v/v) TET System–approved FCS for U–2–OS reporter cell lines (631106, Takara Bio). U–2–OS-pEP15 cells (5 ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Synthetic Biology 2020Quote: ... dry milk and 0.1 % (v/v) Tween 20 and then incubated with mouse anti-6xHis tag monoclonal antibody-HRP conjugate (Clontech or Thermo Fisher Scientific, USA) in the same buffer for 2 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: Using the Cogent NGS Analysis Pipeline software v 1.5.1 (Takara), FASTQ files containing all indices for each chip were demultiplexed to FASTQ files containing one index per file ...
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bioRxiv - Microbiology 2021Quote: ... Following hybridization with ExpressHyb solution (Takara) membranes were washed 4 times and visualized by autoradiography ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with Lenti-X solution (Takara) and concentrated by centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... 2% (w/v) glucose unless specified and the appropriate dropout (Takara Bio) solution for selection ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2% (w/v) glucose unless specified and the appropriate dropout (Takara Bio) solution for selection ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1%Triton X) and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). For the Sera-Mag™ Select (29343052 ...
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bioRxiv - Microbiology 2022Quote: ... and T4 (C) were determined by digerstion with QuickCut™ EcoR V (Takara), MspJI (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... AAV particles were isolated using AAVPro extraction solution (Clontech). After transduction with both CRISPR/Cas9 gesicles and AAV homology donor ...
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bioRxiv - Cancer Biology 2023Quote: ... 12 µg RetroNectin solution (TaKaRa Biomedicals, cat# T100A/B) in PBS was added to each well of untreated 6-well plates (Greiner ...
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bioRxiv - Microbiology 2022Quote: ... Brain tissues from parrots were homogenized using a BioMasher V instrument (TaKaRa, Shiga, Japan) and centrifuged at 1,500 rpm for 10 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... in buffer solution (10xLow salt buffer, Takara Bio Co. Ltd.) and 5 folds dilution by de-ionized water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Positive controls were treated with 50 μL DNase solution (TaKaRa) for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then blocked in ExpressHyb hybridization solution (Clontech) for 1 hour.
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted using Solution 1 (Takara, NKB-101). Secondary antibodies used for immunoblotting included peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... and concentrated 10-fold via Lenti-X Concentrator solution (Takara). Retroviral solution was added to WT ...
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bioRxiv - Cell Biology 2021Quote: RNA-seq libraries for gene expression were constructed using Clontech SMARTer v.3 kit (Takara). Small RNA libraries were constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... were used to prepare RNA-Seq libraries using the SMART-Seq v.4 Kit (Clontech) as previously described42 ...
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bioRxiv - Molecular Biology 2024Quote: ... pLenti V HELLS WT or Mutant plasmids were constructed using In-Fusion cloning (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... A 16 μg/ml solution of Retronectin (T100B; Takara Bio Inc.) was prepared in PBS and stored at −20 °C ...
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bioRxiv - Immunology 2021Quote: ... dNTP solution (10 mM each) and 10X SMARTScribe Reverse Transcriptase (Clontech) was added to the primer-annealed total RNA reaction and incubated for 60 min at 42°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysozyme solution and Protease Inhibitor Cocktail (Takara Bio USA, Inc., CA). The suspension was sonicated 10 sec for 3 times with a 30 s pause ...
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bioRxiv - Molecular Biology 2024Quote: - IgG detector solution V2 (1:2000 dilution, Takara, Cat# T7122A-1).
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bioRxiv - Molecular Biology 2024Quote: IgG detector solution V2 (1:2000 dilution, Takara, Cat# T7122A-1).
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bioRxiv - Immunology 2023Quote: ... RNA was then amplified using SMART-Seq v.4 Ultra Low Input RNA kit (Clontech, cat. 63488). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... the crude viral solution was concentrated using Lenti-x concentrator (Takara, cat.631232) to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were pre-hybridised at 50 °C in ExpressHyb Hybridization Solution (Clontech, USA) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Hybridisation was performed overnight at 50 °C in ExpressHyb Hybridisation Solution (Clontech, USA). After hybridisation ...
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bioRxiv - Bioengineering 2020Quote: ... and lentiviral vectors were concentrated 50-fold using Lenti-X concentrator solution (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... After cross-linking membranes were pre-hybridised in ExpressHyb Hybridization Solution (Clontech, USA) at 40 °C for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... where the lysing solution is composed of single-cell lysis buffer (Takara #635013): 1x RNase Inhibitor Murine (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a high-salt solution for precipitation (Takara Bio Inc., Ohtsu, Japan) and reverse transcribed with ReverTra Ace (Toyobo) ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were UV cross-linked and pre-hybridized with ExpressHyb Hybridization Solution (Clontech) for 1 h at 50°C ...
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bioRxiv - Molecular Biology 2023Quote: ... media was removed and concentrated using lenti-X concentrator solution (Takara Bio #631232). Concentrated viral particles were distributed into single-use aliquots and stored at −80°C until use ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with Takara POD conjugate solution and DAB substrate (Takara, Japan).
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bioRxiv - Molecular Biology 2024Quote: ... were hybridized with specific 32P-labeled probes (Table S3) in ExpressHyb solution (Clontech) for 2 hours at 37°C ...