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Citations for Takara Bio :
901 - 950 of 2594 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, cat# RR096A) and the following primers ...
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bioRxiv - Plant Biology 2019Quote: Y1H assays were carried out using the Matchmaker Gold yeast one hybrid Library Screening System (Biosciences Clontech, Palo Alto, CA, USA). The PRH1 promoter was inserted into the pAbAi vector ...
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bioRxiv - Molecular Biology 2021Quote: ... One ng of DNA was used for real-time qPCR analysis using Premix Ex Taq DNA polymerase (Takara, cat. no. RR039W) on ABI Fast 7500 (Applied Biosystems® ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit) (Takara, Japan) analysis for detection of changes in expression of NQO1 mRNA according to the manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... qRT-PCR was performed by using One Step SYBR® PrimeScript® PLUS RT-RNA PCR Kit (TaKaRa Biotechnology, Dalian, China). Relative expression levels were analyzed by using the 2−ΔΔCT method.
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bioRxiv - Microbiology 2020Quote: ... then subjected to quantitative real-time RT-PCR using a One-Step SYBR PrimeScript RT-PCR kit II (Takara Bio, Japan). The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38 ...
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bioRxiv - Microbiology 2021Quote: ... First the coding region of Candid #1 RdRp was amplified using RNA extracted from P0 or P11 viruses and the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio). Kozak sequence ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of total RNA for each sample was reversely transcribed into cDNA by a commercial kit (RR047A, Takara Bio, Japan) according to the manufacturer’s protocol ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: The inducible cell lines expressing WT or mutants of vimentin were established by the Tet-One Inducible Expression System (Clontech, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... NM_001123007) was amplified from 24 hpf embryo RNA using the PrimeScript II High Fidelity One Step RT-PCR kit (Takara, R026A) and the following primers ...
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bioRxiv - Microbiology 2022Quote: ... into which all possible combinations of 36 assays and 144 samples (127 woodchip samples, 16 standards, and one no-template control) were loaded by the SmartChip MultiSample NanoDispenser (Takara Bio). In brief ...
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bioRxiv - Microbiology 2022Quote: ... the viral RNA was isolated and amplified by RT-PCR using the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio) and specific primers ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription and the first round PCR were performed in a 25μl reaction volume by using PrimeScript™ One-Step RT-PCR Kit (TAKARA, RR055A). Cycling conditions were as follows ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... as previously described (17): pcDNA3.1+-based plasmids used for ectopic expression and pRetroX-tight-Puro-based ones (Clontech, cat. PT3960-5) used here to generate stable cell lines expressing ISG20 upon induction with doxycycline (dox.) ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using a One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in a single-step using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara Bio). The HCoV-OC43 nucleocapsid gene was amplified with the following primers ...
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bioRxiv - Microbiology 2023Quote: ... and quantified by RT‒qPCR using Primer/Probe N2 (NIHON GENE RESEARCH LABORATORIES, INC. Miyagi, Japan) and One Step PrimeScript™ III RT‒qPCR Mix (TaKaRa) with CFX96 (Bio-Rad ...
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... 90 μl of RNase free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara, Cat# RR096A) and primers for Nucleocapsid (N ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 µg cDNA (Takara, Clonetech, USA) was prepared as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 2 × 106 Lenti-X 293T cells (Clontech) were seeded in 6-well plates in 1.5 ml DMEM (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... 2 U/μl RNase Inhibitor (Clontech/TaKaRa). We generated single cell RNA-Seq libraries using a modified Smart-seq2 method (Ding et al. ...
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bioRxiv - Neuroscience 2019Quote: ... 2 U/μl RNase Inhibitor (Clontech/TaKaRa). We generated single cell RNA-Seq libraries using a modified Smart-seq2 method (Ding et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... The Advantage GC 2 PCR Kit (Takara) was used for gene cloning ...
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bioRxiv - Bioengineering 2019Quote: ... using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to the target sequence on M ...
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bioRxiv - Bioengineering 2019Quote: ... using the Advantage 2 Polymerase kit (Clontech) and specific primers located on either side of the target locus ...
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bioRxiv - Microbiology 2020Quote: ... or Advantage 2 Polymerase mix (Takara Bio) for amplification of UTRs and Pfmyob ...
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bioRxiv - Genomics 2019Quote: ... and an Advantage 2 PCR Kit (Clontech) were used for cDNA generation ...
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bioRxiv - Zoology 2021Quote: ... combined with 2 × GC buffer (RR02AG, TaKara) were used for amplification ...
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bioRxiv - Immunology 2020Quote: ... 25 μL 2×PrimeSTAR GC buffer (TaKaRa), 0.5 μL PrimeSTARWHS DNA polymerase (2.5 U/μL ...
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bioRxiv - Microbiology 2023Quote: ... anti-E-cadherin (clone ECCD-2) (Takara), goat anti-rat488 (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the Advantage 2 Polymerase kit (Clontech) and specific primers located on either side of the target locus ...
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bioRxiv - Developmental Biology 2023Quote: ... Advantage 2 polymerase (TaKaRa Bio, Shiga, Japan), or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... and 2 µL Smartscribe Reverse Transcriptase (Takara). RNA mixtures were combined with the RT reaction mixture ...
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bioRxiv - Neuroscience 2023Quote: ... were transiently cotransfected with a 2:1 mixture of Nav1.7-expressing plasmid8 and a pIRES2-ZsGreen bicistronic plasmid (Takara Bio, San Jose, CA) expressing ZsGreen and mouse FHF2B proteins.10 The same pIRES2-ZsGreen plasmid without FHF2 coding sequence served as the control ...
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bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...
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Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylationbioRxiv - Biophysics 2019Quote: Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag ...
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bioRxiv - Immunology 2021Quote: ... T cell receptor sequencing libraries were prepared with the SMARTer Human TCR α/β Profiling Kit (catalog number 635015, Takara Bio USA, Inc.) according to manufacturer’s instructions with the exception of excluding the third and fourth bead size selection steps listed in Table 3 of the kit manual ...
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bioRxiv - Plant Biology 2020Quote: One µg of total RNA was used to generate cDNA with the SMARTer PCR cDNA Synthesis kit (Clontech, Mountain View, CA, USA). Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... LINC00662 (NR_027301) and ELK4 (NM_001973.4) were detected by One-Step SYBR PrimeScript RT-PCR Kit (Perfect Real Time; Takara Bio, Inc., Kusatsu, Japan). Relative expression values were calculated using the relative quantification (2−△△Ct ...
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bioRxiv - Plant Biology 2020Quote: ... and one microgram of the DNase-treated RNA was used for cDNA synthesis using PrimeScript RT reagent kit (Takara Bio, CA, USA). The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA were amplified from the embryo RNA using PrimeScript™ II High Fidelity One Step RT-polymerase chain reaction (PCR) Kit (Takara, R026A) using the following primers ...
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bioRxiv - Plant Biology 2022Quote: ... One base of 5’ dA overhang was added to the PCR amplicon of pMYB93 using Taq polymerase (Takara Bio Inc. Shiga, Japan), which was then cloned into pENTR5’-TOPO (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed using a One-Step SYBR Green Prime ScriptTM RT-PCR Kit II (Perfect Real Time, Clontech, Takara, Kyoto, Japan) with a Thermal Cycler Dice™ system (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed using a One-Step SYBR Green Prime ScriptTM RT-PCR Kit II (Perfect Real Time, Clontech, Takara, Kyoto, Japan) with a Thermal Cycler Dice™ system (Takara ...