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Citations for Takara Bio :
701 - 750 of 1209 citations for Human METTL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... the promoter was inserted into pRS41H-lacZ plasmid using an In-Fusion kit (Takara Bio USA Inc.). The pRS41H-lacZ plasmid was constructed by inserting the lacZ fragment amplified from the pSH18-34 plasmid into the pRS41H plasmid (Estojak et al ...
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bioRxiv - Developmental Biology 2020Quote: ... The two DNA fragments were then ligated into one plasmid with the InFusion HD cloning kit (Takara).
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bioRxiv - Neuroscience 2020Quote: ... The APP-mCherry plasmid was generated by introducing the APP751 sequence in the pmCherry-N1 vector (Clontech) at the XmaI/AgeI restriction site ...
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bioRxiv - Cell Biology 2019Quote: ... CDCP1 and its respective mutants were subcloned into either the pEGFP-N1 or pmCherry-N1 plasmid (Clontech) and then subcloned into the pRetroX-TRE3G retroviral plasmid (Clontech) ...
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bioRxiv - Neuroscience 2022Quote: ... were amplified by PCR and inserted into a CAG-promoter plasmid backbone by In-Fusion HD (Takara). A plasmid encoding hWDR62-FLAG was generated by amplifying hWDR62-FLAG by PCR ...
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bioRxiv - Plant Biology 2022Quote: ... The downstream genomic region was then amplified using the primer set MpHEC1_CDS_IF_F and MpHEC1_3UTR_IF_R2 and integrated into the first binary plasmid with an In-Fusion HD Cloning Kit (Clontech) using the BamHI and AscI sites ...
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bioRxiv - Biochemistry 2022Quote: ... pDONR/Zeo (Supplementary Table 1) plasmid with the desired mutation was done via In-Fusion technology (Takara). Each pDONR/Zeo Cac1 mutant plasmid was verified by Sanger sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... pDESTTM32RAPTOR1BcDNAwas co-transformed either with pDESTTM22FLZ14cDNA or pDESTTM22S6K1cDNA plasmids into the yeast strain Y2HGold (Takara Bio, USA) as previously described (Paiano et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... FD0564)-digested pCRIS-PITChv2-FBL plasmid (addgene #63672) using the In-Fusion HD cloning kit (Takara Bio, 639650). To create the sgRNA-plasmid backbone ...
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bioRxiv - Bioengineering 2022Quote: Arc DNA plasmids were transfected into the donor cell culture via PEI or Xfect transfection reagent (Takara) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The plasmids of EcOpgD and EcOpgG mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Immunology 2023Quote: ... The fragments were gel purified and then assembled into a plasmid using in-fusion reagents (Takara Bio). The mCherry11-RNase L and mCherry1-10-RNase L were made similarly with oligos in Supplementary table 1 ...
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bioRxiv - Genetics 2023Quote: ... Plasmids that contain each sgRNA were transfected to HEK293T cells using Lenti-X packaging single shots (Takara) for viral packaging ...
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bioRxiv - Microbiology 2023Quote: The plasmid constructs used as repair template for recombinant virus generation were generated using InFusion cloning (TaKaRa). The sequence references below are based on the HSV-1 KOS genome accession number JQ673480 (Macdonald et al 2012) ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were generated using Gibson assembly and In-Fusion cloning according to manufacturer protocols (Takara, 638920). PB-NCT-GFP plasmids were generated using NCT plasmids donated by Zane Zeier and were flanked by PiggyBac transposon arms ...
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Molecular Biology 2024Quote: ... We then introduced into this 303_p3E_GI_PA our previous RNAi cassette from plasmid pME-RNAi6415 using an “In-fusion (Takara)” cloning strategy ...
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bioRxiv - Molecular Biology 2021Quote: ... was co-transformed with GAL4 DNA-BD-fused IPMK and a human brain cDNA activation domain (AD) library (Clontech). Two different reporter genes (HIS3 and ADE2 ...
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bioRxiv - Neuroscience 2020Quote: ... we used Sanger sequencing of cDNA reverse transcribed from pooled human hippocampus poly-A-selected RNA (Takara/Clontech 636165) to support the presence of the human-specific intron-3 retention event identified within APOE (GRCh38 ...
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bioRxiv - Neuroscience 2020Quote: ... we used Sanger sequencing of cDNA reverse transcribed from pooled human hippocampus poly-A-selected RNA (Takara/Clontech 636165) to support the presence of the human-specific intron-3 retention event identified within APOE (GRCh38 ...
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bioRxiv - Cell Biology 2021Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan). Expression profiles of bile duct epithelial cells were obtained from Gene Expression Omnibus (GSM4454532 ...
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bioRxiv - Cell Biology 2021Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan). Expression profiles of bile duct epithelial cells were obtained from Gene Expression Omnibus (GSM4454532 ...
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bioRxiv - Biophysics 2022Quote: Experiments were performed using PtK2 GFP-α-tubulin cells (stable line expressing human α-tubulin in pEGFP-C1, Clontech Laboratories ...
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bioRxiv - Genomics 2022Quote: ... IgG and IgM 5’RACE AIRR-seq libraries were generated using the SMARTer Human BCR Profiling Kit (Takara Bio), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Human PDE4D5 wild type and PDE4D5-dN mutant were PCR amplified and cloned into pLVX-mCherry-N1 vector (Clontech) using Gibson assembly after vector digestion with XhoI and EcoRI ...
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bioRxiv - Cell Biology 2020Quote: EGFP-Rab5 construct was generated by PCR amplifying human Rab5a from cDNA and subcloning into the pEGFP-C2 (Clontech) vector using XhoI/BamHI restriction sites ...
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bioRxiv - Microbiology 2019Quote: ... Sequences encoding primate PBMs were added to the resulting pDONR221-mCherry-human GBP1DC_BglII vector following BglII digestion using Ligation-Independent cloning (In-Fusion, Clontech) with annealed oligomers that also restored human GBP1 Q580-L581 and the human GBP1 CaaX box (Table S9) ...
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bioRxiv - Biophysics 2021Quote: ... PCR products were cloned into vector pcDNA3.3 (Thermo Fischer Scientific) for human IgG1 expression using In-Fusion cloning technology (Clontech) as described previously[1] ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were produced by transient transfection of human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) using the JetPrime transfection kit (Polyplus Transfection Illkirch ...
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bioRxiv - Molecular Biology 2021Quote: ... Human eRF1 coding sequence was cloned into the XhoI and HindIII sites of the pλN-HA-C1 vector (Clontech). The pλN-HA-C1-eRF1 plasmid was then used to generate the pλN-HA-C1-eRF1AAQ (G183A G184A ...
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bioRxiv - Cell Biology 2021Quote: ... US) and human cervical adenocarcinoma cells expressing a tetracycline-inducible repressor (HeLa Tet-Off, Clontech, Mountain View, California, US) were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: The EGFR-GFP plasmid was constructed using the cDNA of human EGFR (pNeoSRαII) provided by Akihiko Yoshimura (Keio University) and was inserted into the pEGFP-C1 vector (Clontech) with the same linker sequence suggested by Carter and Sorkin (Carter and Sorkin ...
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bioRxiv - Genomics 2023Quote: 200ng of RNA extracted from whole human blood used as input for reverse transcription using Smartscribe RT (Takara Clontech) with primers targeting the IGH constant regions (IDT ...
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bioRxiv - Genomics 2023Quote: 200ng of RNA extracted from whole human blood used as input for reverse transcription using Smartscribe RT (Takara Clontech) with primers targeting the IGH constant regions (IDT ...
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bioRxiv - Cancer Biology 2023Quote: ... Individual lentiCRISPRv2 vectors were introduced along with packaging vectors into human embryonic kidney (HEK) 293T cells via calcium phosphate transfection according to the manufacturer’s protocol (Clontech). Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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bioRxiv - Cell Biology 2020Quote: Plasmids were constructed using standard molecular biology methods including polymerase chain reaction (PCR) and In-fusion cloning (Clontech). Mutations for specific residues were introduced by overlap-extension PCR ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids encoding the bait (pDEST32) and prey (pDEST22) were transformed into the yeast strain AH109 and Y187 (Clontech) by the LiAc method following the protocol in the MATCHMAKER GAL4 Two Hybrid System 3 manual (Clontech) ...
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bioRxiv - Genetics 2019Quote: ... the BirA-R118G open reading frame from the BirA(R118G)-HA destination vector (gift from Karl Kramer (plasmid # 53581, http://www.addgene.org) was subcloned into the retroviral vector pQCXIN (Clontech) to generate either N-terminal (pQCXIN-BirA-Myc-N ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid called “CFP-Golgi” (containing amino acids 1–81 of ß-1,4glycosyltransferase) was originally purchased from Clontech.
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bioRxiv - Systems Biology 2021Quote: ... Other plasmid components (see Supplementary Table 1) were synthesized or PCR amplified with PrimerSTAR MAX DNA polymerase (TAKARA) and checked by Sanger sequencing ...
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bioRxiv - Biochemistry 2020Quote: The full-length L1 fragments of 15 high-risk HPV (hrHPV) were cloned in the pMD plasmid (Takara) to prepare HPV plasmids ...
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bioRxiv - Biochemistry 2020Quote: ... the cDNA encoding Halo7-β2AR-FKBP_F36V (β2AR-FKBP) was cloned into the expression plasmid vector pEGFP-N1 (Clontech) after the removal of the cDNA region encoding EGFP ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid pIRESpuro3-EGFP was constructed by excising the EGFP open reading frame from pEGFP-C1 vector (Clontech) with NheI and BamHI digestion and insertion into the same restriction sites of the pIRESpuro3 vector (Clontech ...
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bioRxiv - Neuroscience 2020Quote: ... a series of FRT-FP plasmids were constructed (Fig. S1) using the mammalian expression backbone pCMV-N1 (Clontech). FRT-F13 ...
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bioRxiv - Neuroscience 2020Quote: ... #AM9937).The Brp-T2A-QF2w-Hsp70-3XP3-dsRed donor plasmid was constructed using the InFusion HD Kit (Clontech, #638910). Homology arms of ∼1 kb flanking the sgRNA cut site were cloned by PCR using template genomic DNA from the Orlando strain ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA to generate viral vectors was prepared using the NucleoBond Xtra Maxi endotoxin free kit (Takara Bio) prior to production ...