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Citations for Takara Bio :
551 - 600 of 1209 citations for Human METTL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... VAMP2-tagRFP was generated by cloning from VAMP2-pHluorin into the tagRFP plasmid (Clontech). VAMP21-96-tagRFP was created by PCR of VAMP2 (AA 1-96 ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid used for disruption was constructed using the In-Fusion cloning kit (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids were co-transformed pairwise into AH109 yeast strains (Matchmaker 3 System, Clontech), and selected initially on double drop-out (DDO ...
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bioRxiv - Neuroscience 2022Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with PHP.B capsid (Ben Deverman ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of plasmids were diluted in 200 µL Xfect transfection reagent (631317, Takara). The mixture was incubated at room temperature for 15 min ...
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bioRxiv - Genetics 2022Quote: ... Cas9 was amplified from the expression plasmid using CloneAmp HiFi PCR Premix (Takara Bio) for 35 cycles (98□ for 10 s ...
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bioRxiv - Molecular Biology 2023Quote: ... were subcloned in frame from their corresponding SMAUG1-HisTEV2xFLAG plasmids into pEGFP-N1 (Clontech) using XhoI and BamHI ...
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bioRxiv - Synthetic Biology 2023Quote: ... here we inserted the two chaperones GroES and GroEL from the pGro7 plasmid (TaKaRa). The sequence for GroES and GroEL were amplified from the pGro7 plasmid by PCR and inserted into the linearized plasmid with NpnJ by In-Fusion® ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids used in this study were constructed using In-Fusion® Cloning Technology (TaKaRa). Primers and genes were ordered from Integrated DNA Technologies (IDT) ...
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bioRxiv - Biochemistry 2023Quote: The plasmid library was transformed into Escherichia coli strain BL21(DE3)-pGro7/GroEL (TaKaRa). Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™ ...
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bioRxiv - Bioengineering 2023Quote: ... and AAV6 were prepared using the AAVpro® Packaging Plasmid (Takara Bio, Shiga, Japan), while AAV4 ...
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bioRxiv - Genomics 2023Quote: ... Lentivirus containing the overexpression plasmid was generated using LentiX Packaging Single Shots (Clontech, 631278) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were transformed into Escherichia coli BL21 (Takara Bio Co Ltd, Japan) and the transformed cells were cultured at 37°C until the OD600 reached at 0.6–0.8 ...
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bioRxiv - Genomics 2023Quote: ... cells were co-transfected with 1 µg of pEGFP-N1 plasmid (Takara Bio USA). Where indicated ...
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bioRxiv - Plant Biology 2023Quote: ... Purified PCR products were cloned into pYAO::hSpCas9 plasmid via In-Fusion (Takara, 639650).
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bioRxiv - Cell Biology 2023Quote: All plasmids used in this study were generated using the Infusion cloning system (Takara) with primers containing 5’ flanking regions (15bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid extraction was performed using the NucleoBond Xtra Midi EF kit (Takara Bio, 740420.10).
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bioRxiv - Biophysics 2023Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with either the AAV2/9 ...
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bioRxiv - Immunology 2023Quote: ... As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories, USA) was transfected.
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bioRxiv - Cell Biology 2023Quote: ... we used the pRetroX-Tet-on-advanced and pRetrox-Tight-Puro plasmids from Clontech Inc ...
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bioRxiv - Bioengineering 2024Quote: cDNA and the plasmid library were amplifed using CloneAmp PCR Master Mix (Takara 639298) and primers for amplicon sequencing (Supplemtary Note 23) ...
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bioRxiv - Bioengineering 2019Quote: The promoter of the HSPA6 gene (Uniprot P17066) was amplified from human genomic DNA (Clontech #636401) from −1231 bp to +119 bp relative to the transcriptional start site ...
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bioRxiv - Biochemistry 2019Quote: Human LRAT cDNA was synthesized and cloned into a pcDNA3.1(+) vector (Clontech, Palo Alto, California, USA) by a third party (GeneArt ...
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bioRxiv - Cancer Biology 2019Quote: ... The human patient PDX cell lines were maintained in RPMI-1640 medium containing 10% FBS (Clontech). KRAS mutation status ...
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bioRxiv - Biochemistry 2020Quote: ... Human IFITM3 cDNA was purchased from Open Biosystems and PCR cloned into pCMV-HA vector (Clontech). All mutants of IFITM3 were generated by using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... for the samples from the mouse heart or Human Mitochondrial DNA Monitoring Primer Set (TaKaRa Bio) for the cells following manufacturer protocol.
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bioRxiv - Genomics 2022Quote: ... 100 ng of polyA selected RNA from the human lung carcinoma cell line A549 (Takara #636141) was used as input ...
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bioRxiv - Physiology 2022Quote: ... the cDNAs of full length human GCNA or IDR hGCNA were cloned into pEGFP-C1 (Clontech) between BglII and SalI sites ...
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bioRxiv - Genetics 2019Quote: Regions orthologous to human placental igDMR were PCR-amplified with TaKaRa EX Taq HS (TaKaRa Bio) with primers specific for chimpanzee and rhesus macaque CGIs (Supplementary Table 4) ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs for C-terminal GFP-tagged human RHBDL4 were generated by subcloning into pEGFP-N1 (Clontech). For generating point mutants ...
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bioRxiv - Cell Biology 2020Quote: Human sparc cDNA clone was described by us before 29 and subcloned into pShuttle vector (Clontech). Adenovirus expressing human SPARC was constructed using Adeno-X expression system (Clontech ...
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bioRxiv - Cell Biology 2021Quote: ... OCIAD1 was initially cloned from human cDNA into a pAcGFP-N1 vector (Clontech, Mountain View, CA). The GFP1-10 vectors were cloned by Gibson assembly into a FUGW lentiviral backbone (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... Human cDNA for α7-nAChRs and NACHO inserts were cloned using In-fusion cloning kit (Takara). After cloning in the lentiviral transfer plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... the cDNA encoding human Wt-syn was introduced into a vector from Clontech (Mountain View, CA) containing the mRFP sequence ...
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bioRxiv - Cancer Biology 2023Quote: PDGF-IRES-Cre was generated by cloning human PDGF-B and Cre into pQXIX vector (Clontech) as previously described5 ...
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bioRxiv - Biophysics 2023Quote: Human cell lines used in this study include U2OS and Lenti-X 293T (Takara Bio USA). Lenti-X 293T cells were only used for virus production ...
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bioRxiv - Immunology 2023Quote: ... The human J chain was replaced with the DsRed2 gene of pDsRedN1 (Takara Bio, Shiga, Japan). The pIgR gene was inserted into the pEF-Myc-His vector (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Restriction digestion of plasmid DNA with ECoRI in Takara RE kit (Clontech Bio Inc, Japan) was done to further confirm successful cloning of Pghsp17.0 gene from Pearl millet cultivar WGI 126.
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bioRxiv - Cell Biology 2021Quote: ... The XPack-GFP plasmid was generated by inserting GFP from pEGFP-N1 (BD Biosciences Clontech) into the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector (System Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: Plasmid expressing mCherry-myc-TRIM1 was created by cloning myc-TRIM1 into pmCherry-C1 (Clontech). Plasmid expressing EBFP2-14-3-3 theta was created using HiFi Cloning (NEB ...
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bioRxiv - Biophysics 2022Quote: ... This plasmid expresses the PR isoform-B under a tetracycline controllable promoter (TetOff system, Clontech). To perform the SPT experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... Supercoiled pHSG299 plasmid dsDNA purified by CsCl-ethidium bromide gradient centrifugation was purchased from Takara Bio Inc ...
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bioRxiv - Microbiology 2020Quote: DNA encoding the eGFP sequence was PCR amplified out of the eGFP-N2 plasmid (Clontech) and subcloned via single-cut restriction digest into the corresponding UL25 plasmid harboring the cleavable His6-SUMO tag [(either UL25Δ50 (pED13 ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products were inserted into the respective plasmids using the In-Fusion cloning system (Clontech), the sequence- and ligation-independent cloning (SLIC ...
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bioRxiv - Cell Biology 2019Quote: ... followed by PCR-mediated amplification and plasmid insertion with the in-Fusion cloning kit (Clontech). The CLN6ΔL2 construct was obtained by removing the codons for amino acids 155-222 using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Enzyme was then expressed in BL21-DE3 cells with pG-KJE8 chaperone plasmid (Takara Bio) in media supplemented with 0.5 mg/mL arabinose and 5 ng/mL doxycycline to induce chaperone expression ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid was then submitted to site-directed mutagenesis using the In-Fusion technology (Takara Bio Europe ...
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bioRxiv - Plant Biology 2020Quote: ... Combinations of LexA BD- and AD- fusion plasmids were cotransformed into yeast strain EGY48 (Clontech) containing the reporter plasmid p8op-LacZ ...
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bioRxiv - Cell Biology 2021Quote: ... C-terminally triple FLAG tagged MBP was inserted into pKK plasmids using InFusion cloning (Takara). To introduce C-terminally triple FLAG tagged UPF1 ...
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bioRxiv - Cell Biology 2022Quote: ... All plasmids during this study were made with In-Fusion HD Cloning Plus (Takara, 638909). All yeast strains are isogenic to BY4741 ...