Labshake search
Citations for Takara Bio :
651 - 700 of 4863 citations for Mouse CXCL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... using an In-Fusion HD Cloning Kit (Takara; #Z9649N). pCANw-HA-cZO1-Flag was created by inserting an HA tag into the N-terminus of cZO-1 by PCR using the following primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the KOD Xtreme Hot Start DNA polymerase kit (Takara) was utilized ...
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bioRxiv - Microbiology 2023Quote: ... and the PrimeScript cDNA synthesis kit (TaKaRa, Shiga, Japan) with 400 ng RNA input in a 10 μl reaction ...
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bioRxiv - Plant Biology 2023Quote: ... by using In-Fusion HD Cloning Kit (Takara Bio). Amino acid substitutions were introduced into pMAL-c5X Raf13 by site-directed mutagenesis to produce the kinase-dead Raf13 (K546N) ...
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bioRxiv - Genetics 2023Quote: ... sgRNA was produced using sgRNA IVT kit (Takara Bio) by following the manufacturer’s instructions and RNA was isolated by Trizol (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNAs were produced by sgRNA IVT kit (Takara Bio) by following the manufacturer’s instructions and RNA was isolated by Trizol (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The NucleoBond Xtra BAC kit was purchased from TaKaRa Bio (Kusatsu ...
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bioRxiv - Microbiology 2023Quote: ... and the Premix Ex Taq (probe qPCR) kit (Takara). All PCRs were performed in a StepOne plus instrument (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan). For the cloning of the sec11a (NM_014300.3 ...
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bioRxiv - Cell Biology 2022Quote: ... SMART-Seq® HT PLUS Kit (Takara, Kusatsu, Japan) was used to build mRNA libraries ...
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bioRxiv - Developmental Biology 2022Quote: A Smart-seq v4 3’ DE Kit (Takara Bio) was adapted to DRaqL as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA levels were measured using qRT-PCR kit (Takara) in combination with TB Green real-time PCR ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using the In-Fusion® HD Cloning Kit (Takara Biomedical Technology Co. ...
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bioRxiv - Developmental Biology 2023Quote: ... the PrimeScript Reverse Transcriptase kit (TaKaRa, http://www.takara-bio.com). And the quality of total RNA was detected by NanoDrop ND-1000 (NanoDrop ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was then synthesized with cDNA Synthesis Kit (Takara) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... using a SYBR Green Ι Master mix kit (Takara). The mean value ± SE was calculated for three biological replicates for each experiment.
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bioRxiv - Cell Biology 2023Quote: ... the SMARTer RNA unique dual index kits (Takara 634452) were used instead of the primers that came with the SMARTer Stranded Total RNA HI Mammalian kit to allow for sequencing on the NovaSeq (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated using Double Primed EcoDry kits (Takara). The qPCR reaction was carried out using PowerUp SYBR Green (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... or pUC118 vectors (Mighty Cloning Kit; Takara, Kusatsu, Japan). The intact env gene was inserted into the pFUΔss expression vector (51) ...
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bioRxiv - Molecular Biology 2023Quote: ... or pIExZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2023Quote: ... Cloning was performed with In-Fusion cloning kit (Clontech). The list of primers is available in the Supplementary Table S1.
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bioRxiv - Cancer Biology 2023Quote: ... the High-Capacity cDNA Reverse Transcription Kit from TAKARA was used ...
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bioRxiv - Genomics 2023Quote: ... or a Takara NucleoSpin RNA Plus kit (Takara, 740984) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... the oligo dT-based mRNA isolation kit (Takara Bio) was employed ...
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bioRxiv - Cell Biology 2024Quote: ... facilitated by the PrimeScript RT regent Kit (TaKaRa, Japan). Purification of the synthesized second-stranded DNAs was accomplished using AMPureXP beads ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated using PrimeScript RT kit (Takara RR037A) with 100-500 ng RNA as input ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: Capturem Co-IP kit was procured from TaKaRa (Japan). The Neuro-2a cells were transfected with pEGFP-PKM2 and cells were infected with JEV 24 hours post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... packaging plasmid using the CalPhos Mammalian Transfection Kit (Takara). The next day ...
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bioRxiv - Genomics 2024Quote: ... using the In-Fusion HD Cloning Kit (Takara Bio) and following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using the Advantage® 2 PCR Kit (Takara Bio) in a touchdown cycling program as follows ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was prepared from EV RNA using the Single Cell RNA kit SMART-Seq_V4 Ultra Low Input RNA Kit (634890, Takara, Mountain View, CA, USA) according to manufacturer instructions using 1ng input ...
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bioRxiv - Developmental Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from a pooled female pupal RNA sample and also from a pooled adult female ovary sample (RNA extracted using RNeasy MinElute kit, RACE conducted using SMARTer 5⍰/3⍰ RACE kit - Takara Bio, Kyoto, Japan). Visible bands were cloned using the NEB PCR cloning kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... Both types of DNA fragment (0.1 µg) were used as templates to synthesize RNA using an in vitro transcription kit (in vitro Transcription T7 Kit; Takara Bio, Shiga, Japan) in 1.5 mL centrifuge tubes ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized with a PrimeScript™ RT synthesis kit with one-step genomic gDNA eraser kit according to instructions (TaKaRa, Tokyo, Japan). Gene-specific primer sets were designed based on the ORF ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted with a Bacterial RNA Extraction Kit and was immediately reverse-transcribed into cDNA using a PrimeScriptT™ RT-PCR Kit (TAKARA, Daliang, China). The common primer pair GSPilACF/GSPilACR was used to test genomic DNA contamination ...
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bioRxiv - Biochemistry 2023Quote: ... Mutants were generated by site-directed mutagenesis using a KOD-Plus Mutagenesis Kit (Toyobo, Japan) or PrimeSTAR® Mutagenesis Basal Kit (Takara Bio, Japan). For the expression ...
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bioRxiv - Cell Biology 2020Quote: msPLCε cDNA was amplified by PCR from a mouse liver first-strand cDNA library (kindly gifted from Tomohiro Ishii) using Tks Gflex polymerase (TaKaRa) and was cloned between the NheI and KpnI sites of pECFP-N1 (Clontech ...
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bioRxiv - Systems Biology 2022Quote: ... The blocked membrane was incubated overnight at 4 ℃ in primary antibodies: mouse polyclonal anti-Cas9 (Takara Bio, cat. no. 632607), mouse monoclonal anti-β-actin (8H10D10 ...
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bioRxiv - Cell Biology 2019Quote: Expression plasmid for mouse FLAG tagged BAG3 (pFLAG-BAG3) was constructed by cloning partial mouse BAG3 cDNA containing the whole CDS into pEGFP-N1 (Clontech). Forward primer sequence used to clone BAG3 plasmid is 5′-AAAGGATCCAGCGCCGCCACCC-3′ and the reverse primer sequence is 5′-GACTCTAGATCACTAGGGAGCCACCAGGTTGC-3′ ...
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bioRxiv - Genetics 2019Quote: ... liver and kidney), mouse tissues (brain, liver, heart, testis and kidney) and rat tissues (brain, liver and kidney) were purchased from Takara Bio Inc.
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs encoding full-length human HCN1 channel and mouse TRIP8b (splicing variant 1a-4) were cloned into the pcDNA 3.1 (Clontech Laboratories) mammalian expression vector ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then co-transfected with 150 ng of the pBiFC-HA-Casp2(S384E)-VC155 and pBiFC-HA-Casp2(S384E)-VN173 (mouse caspase-2) for BiFC and 10 ng of pDsRed-Mito (Clontech) as a transfection reporter plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... Membrane blocking was performed with 3%BSA in PBS-t buffer for 1 h at room temperature followed by incubation with Mouse-anti-GFP (TaKaRa) (1/5,000 ...
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bioRxiv - Cell Biology 2019Quote: ... of mouse E2F1 gene and the resultant fragment was ligated between the BamH1 and Xba1 sites of the pQXCIP vector (Clontech). pQCXIP/N-m (or h)E2F1p::pE2VF1-3’UTR ...
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bioRxiv - Neuroscience 2021Quote: ... anti-ORF1p (guinea pig, 1/200, in-house, clone 09 as in 83, anti-mCherry (mouse, 1/200, Clontech 632543), rabbit anti-H3K9me3 (rabbit ...
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bioRxiv - Cell Biology 2021Quote: ... pDAA-026 was generated by subcloning a DNA fragment encoding an N-terminus FLAG-tagged mouse eIF2α coding sequence flanked by BamHI and EcoRI sites into the BglII and EcoRI sites of pLPCX (Clontech) using standard molecular biology methods ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were subjected to 4-20% SDS-PAGE and analyzed by immunoblotting with the following primary antibodies: anti-GFP mouse monoclonal antibody (JL-8, Takara), GFP rabbit polyclonal antibody (PABG1 ...
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bioRxiv - Neuroscience 2022Quote: ... These plasmids were obtained by cloning cDNA extracted from mouse cerebellum in pAAV-MCS Expression Vector with In-Fusion Cloning system (Takara). 96h after transfection ...
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bioRxiv - Biophysics 2023Quote: ... A plasmid expressing mouse GR tagged in the N-terminus with mCherry was developed by amplifying mouse GR coding sequence and in-frame cloning in pmCherry-C3 (Clontech). Point mutations and deletions were introduced using the Quickchange XL mutagenesis kit (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... was generated by amplifying full-length mouse ANGPTL4 cDNA (OpenBiosystems) and inserting it into a pCDNA6 vector using In-Fusion cloning (Clontech). A V5 tag was then appended to the C terminus of the open reading frame using Phusion site-directed mutagenesis (New England Biolabs).