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Citations for Takara Bio :
551 - 600 of 5147 citations for Mouse CXCL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... a mouse monoclonal antibody for bovine osteocalcin (code no. M042, clone no. OCG2; Takara Bio Inc., Shiga, Japan), and goat polyclonal antibody for FBXW2 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (anti-Th [mouse] 1:1500, Immunostar; anti-dsRed [rabbit] 1:1500, Clontech) overnight at RT shaking ...
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bioRxiv - Cell Biology 2022Quote: ... which was used to screen a Mate & Plate™ Library-Universal Mouse (Normalized) (Clontech, Mountainview, CA; Cat#: 630482) using the stringent protocol according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Total protein was harvested after 3 days of culture and analyzed by Western blot (mouse anti-DsRed, Clontech; rabbit anti-POU6F2 ...
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bioRxiv - Bioengineering 2024Quote: ... GFP was detected with a monoclonal mouse anti-GFP antibody (JL-8, Takara Bio USA, San Jose, CA) in Western blot analyses.
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bioRxiv - Cell Biology 2023Quote: ... Full-length human CLUH and mouse Cluh coding sequences cDNA were cloned in pcDNA3 and pmCherry-N1 (Clontech), respectively ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were diluted in 5% milk in PBS-T + 0.01% sodium azide (1:2,000 for mouse anti-GFP (Clontech) and mouse anti-3V5 (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: RNA extracion from the cells and mouse hearts was performed using the sophisticated Trizol reagent (Takara, Kyoto, Japan). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: pEGFP-USP48 was generated by sub-cloning the mouse USP48 into the mammalian expression vector pEGFP-C2 (Clontech). pCMV-Myc-USP48 ...
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bioRxiv - Cancer Biology 2024Quote: ... Retroviral transduction was achieved by spinoculation of 3 × 106 mouse T cells on retronectin-coated plates (Takara Bio) with neat retroviral supernatant harvested from 293T packaging cells (2000xg ...
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bioRxiv - Plant Biology 2020Quote: The total RNA of the buds collected for the transcriptome research was extracted by the EASYspin Plus Plant RNA Kit RN37 (Aidlab Biotechnology) and cDNA synthesis by the M-MLV RTase cDNA Synthesis Kit (TaKaRa Company). cDNA amplified fragment length polymorphism (cDNA-AFLP ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from DRG was extracted using RNeasy micro kit and reverse-transcribed using PrimeScript RT Reagent Kit (Cat# RR037A, TaKaRa, Japan). Real-time PCR was performed using the SYBR Premix Ex Taq™ (Cat# DRR041A ...
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bioRxiv - Biochemistry 2019Quote: ... cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits) from Clontech (Mountain View, CA). Specific primers designed according to human ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells by TRIzol and an RNA extraction kit (MiniBEST Universal RNA Extraction Kit, RR820A, Takara, China). Total RNA (1 μg ...
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bioRxiv - Genetics 2020Quote: RNA was extracted using the Biostic Blood Total RNA Isolation Kit sample (MO BIO laboratories, Inc) and cDNA was obtained using the PrimeScript™RT reagent Kit (Takara). RTqPCR gene expression analysis was performed using the Taqman probes HSF2-Hs00988309_g1 and GADPH-Hs02758991_g1 for normalization (Applied Biosystems).
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Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiaebioRxiv - Bioengineering 2019Quote: Total RNA was extracted using MiniBEST universal RNA extraction kit and 1 μg was taken to synthesize cDNA using the PrimeScript II 1st-strand cDNA synthesis kit (TaKaRa, Japan). The cDNA mixture was diluted to about 100 ng/μl and used as the template for the gene expression level analysis by qRT-PCR ...
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bioRxiv - Physiology 2021Quote: ChIP-seq libraries were prepared on 5 ng of DNA from ChIP and input samples using the SMARTer ThruPlex DNA-seq kit along with DNA HT Dual Index Kit (both from Takara Bio) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted directly from the virus stock using the Indimag Pathogen kit (Indical Biosciences) and transcribed to cDNA using the PrimeScript™ RT reagent Kit (Takara) using oligo-dT and random hexamers ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated using the FastGene Plasmid Mini Kit (Nippon Genetics, Tokyo, Japan) or NucleoSpin Plasmid EasyPure Kit (Takara Bio). The REase NdeI was employed for the linearization of plasmid DNAs ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs from scramble shRNA or TDP-43 shRNA treated cells were used to construct RNA-sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit (Takara 634411), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Whole genome amplification was performed on the prepared mixture samples with BioSkryb ResolveDNA™ Whole Genome Amplification Kit (BioSkryb Genomics) and Takara PicoPlex WGA v3 kit (Takara) following the manufacture’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... The viral particles were purified from transfected cells and titrated using the AAVpro Purification Kit Maxi and AAVpro Titration Kit (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and CDS of fluorescent proteins were subcloned into pET-human αSyn51 using the KOD-Plus Mutagenesis kit (TOYOBO) and In-Fusion HD Cloning Kit (Takara Bio) according to the manufacturers’ protocol ...
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bioRxiv - Physiology 2024Quote: cDNA synthesis and Real-Time PCR were performed following the manufacture instructions of the PrimeScript RT reagent Kit with gDNA Eraser kit (Takara, #RR047A). For the Real-Time PCR reaction ...
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bioRxiv - Molecular Biology 2024Quote: RNA that passed the quality test underwent RNA polyadenylation and cDNA synthesis according to the instructions of the kit manufacturer (Mir-X™ miRNA First-Strand Synthesis Kit; Takara). The reaction system was configured according to the real-time fluorescence quantitative PCR user manual (ChamQ Universal SYBR® qPCR Master Mix ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared using SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian kit (Takara Bio USA, Cat.# 634411) and following the user manual (Rev ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian” kit (Takara Bio USA, Cat. # 634411), following “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian User Manual (Rev ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian” kit (Takara Bio USA, Cat. #634411). The protocol was started with 4-10 ng of total RNA ...
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bioRxiv - Developmental Biology 2022Quote: c-Kit-EGFP fusion protein: chicken c-Kit cDNA was inserted into multiple cloning site of pEGFP-N1 Vector (Clontech, #6085-1) including EGFP sequences using a primer set ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were induced by 1 mM IPTG at 16°C for 20 h, then purified by a GST-tag Protein Purification Kit (Beyotime, Shanghai, China) or His TALON Purification Kit (Takara, Beijing, China). Corresponding primers are listed in Supplemental Table S2.
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted with an Axygen RNA purification kit (Capital Scientific) and the first DNA was synthesized from RNA using a PrimeScript RT regent kit (Takara, Shiga, Japan). Actin-coding gene (KP341672 ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... RNA was reverse-transcribed to cDNA by using DNA removal and a cDNA synthesis kit (PrimeScriptTM RT reagent Kit with gDNA Eraser; TaKaRa, Kusatsu, Japan) with a mixture of oligo-dT and random primers ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was removed using the DNA-free kit (Thermo Fischer Scientific, Cat# AMl906) and subsequent cDNA synthesis was performed using the PrimeScript RT reagent Kit (TAKARA, Cat# RR037A), both according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was collected using the Nucleospin RNA Kit (Macherey–Nagel, Hoerdt, France) and transcribed into complementary DNA (cDNA) with the PrimeScript RT Reagent Kit (RR037A, Takara, Kusatsu, Japan). cDNA was then amplified with the SYBR Green Master kit (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... ∼900 bp homology arms to Usp9x were amplified from mouse genomic DNA using PrimeStar GXL polymerase (Takara, CA, USA) and Gibson assembly primers with 21 nucleotide overlap to adjacent fragments ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were transferred to PVDF membrane and blotted proteins were incubated in a 1:10,000 dilution of mouse monoclonal anti-GFP antibody (Living Colors JL-8, Clontech) followed by 1:5000 horseradish peroxidase conjugated sheep anti-mouse IgG antibody (Amersham ...
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bioRxiv - Cell Biology 2022Quote: ... 5um sections from paraffin blocks were used for immunofluorescence staining with the following primary antibodies: tdTomato (dsRed Mouse: Takara Biosystems 632392 ...
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bioRxiv - Neuroscience 2021Quote: ... Three technical replicates of 300 cells each were sorted from each individual mouse into 1.5mL microcentrifuge tubes containing cell lysis buffer buffer from the Clontech SMART-Seq HT (Takara) kit for direct cDNA synthesis and RNAseq library generation.
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bioRxiv - Cell Biology 2019Quote: ... 10 µg of the lysates were separated by SDS-PAGE and blots were probed with a 1:2,000 dilution of mouse anti-GFP antibody (Clontech), followed by 1:5000 anti-mouse Starbright Blue 700 (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... NPR1-GFP proteins were detected by reacting the blots with the mouse monoclonal anti-GFP monoclonal antibody (Clontech, USA) and horseradish peroxidase-conjugated secondary antibody (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... A 5 µl aliquot was removed from each sample for immunoblots using mouse anti-GFP (1:5,000, Clontech #632381) to detect A3-EGFP ...
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bioRxiv - Pathology 2022Quote: ... mouse brains were extracted after cardiac perfusion with cold phosphate buffered saline (PBS) and homogenized by RNAiso Plus (TAKARA) and homemade RIPA buffer ...
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bioRxiv - Cell Biology 2023Quote: ... mouse MEFs (RIKEN BioResource Center, Tsukuba, Japan), mouse RAW264.7 macrophages (RIKEN BioResource Center, Tsukuba, Japan) and Lenti-X 293T (Clontech) were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-nc82 (1:50; Developmental Studies Hybridoma Bank) and rabbit anti-RFP (1:1000; TaKaRa Bio USA, #632496) at room temperature with agitation for 2 days ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently stained using a horseradish peroxidase (HRP)-conjugated monoclonal anti-StrepTag antibody (Iba) or mouse anti-6His (Clontech) monoclonal antibody combined with rabbit anti-mouse-HRP polyclonal antibodies (DAKO) ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using the nucleospin RNA kit (Takara) according to the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... SMART-Seq v4 Ultra Low input RNA kit (Clontech) was used to amplify cDNA which was subsequently sheared resulting in 200-500 bp fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated using PrimeScript RT kit (Takara #RR037A). For semi-quantitative RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... using a Random Primer DNA Labeling Kit (TaKaRa, 6045) according to the manufacturer’s protocol ...