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Citations for Takara Bio :
601 - 649 of 649 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... for A3>P: 5’-aaaacacugaaccug-3’ for A2>P: 5’-aaacacugaaccug-3’) were incubated with 10 U recombinant MazF (Takara Bio) in 20 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2024Quote: ... we used a 2:1 molar ratio of insert to SalI/AgeI-digested pTYC10 (125 ng of pPromALB_STARRseq plasmid; Table S1E) in a single 10 μl In-Fusion HD reaction (Clontech, cat. # 639650) via the homology arms ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentration was assessed by Nanodrop and RNA-seq libraries were prepared from 10 ng of total RNA using the SMARTer Stranded Total RNA-seq Pico Input Mammalian V2 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... PCR experiments were performed in a total volume of 10 μl by using an SYBR Premix Ex Taq kit (Takara Bio). The reaction mixtures were incubated for 2 min at 95℃ ...
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bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...
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bioRxiv - Neuroscience 2021Quote: ... and 45 cycles from 10 s at 95°C to 30 s at 60°C using a TB Green Premix Ex TaqTM GC (Takara Bio, Japan).
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bioRxiv - Neuroscience 2021Quote: ... and 45 cycles from 10 s at 95°C to 30 s at 60°C using TB Green Premix Ex TaqTM GC (Takara Bio, Japan).
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bioRxiv - Genomics 2021Quote: ... the synthesized cDNA products were subjected to real-time PCR in a reaction mixture (10 μl) containing Takara Premix Ex Taq (Probe qPCR) (TaKaRa, Kyoto, Japan), 200 nM hydrolysis probes and 200 nM PCR primers ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells grown in 10 ml of medium (3.0 x 105 cells/ml) were transfected with 10 µg of pCAGGS-NP (1-450) using TransIT-293 Reagent (Takara, Shiga, Japan). Three days post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... vector used was AAV9-CaMKII::G-CaMP7 (9.4 × 1012 vg/mL)35 after 10-fold dilution with phosphate buffered saline (PBS) (T900, Takara BIO INC, Japan) before injection ...
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A New Gene Set Identifies Senescent Cells and Predicts Senescence-Associated Pathways Across TissuesbioRxiv - Cell Biology 2021Quote: ... 86% of 2% Tween 20 in deionized Water) or AP20187 (B/B homodimerizer, Clontech; 10 mg of AP20187 per kg body mass) twice weekly at the age of 20 months for a total of 4 months (old mice were sacrificed at 24 months of age) ...
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bioRxiv - Biochemistry 2021Quote: ... RNC 82-aa C34A with [SG]5 or [SG]10 repeats were constructed by the Prime STAR MAX (Takara Bio Inc., Japan) method using appropriate primers (Table 1).
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bioRxiv - Evolutionary Biology 2021Quote: ... The decanted soluble extract was adjusted to a volume of 90 mL with lysis buffer and incubated with 10 mL TALON cobalt resin (Takara, Shiga, Japan) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... S domains and variants were purified similar to S protein using nickel resin (10 mL/L, His60 Ni2+ superflow resin, Takara cat# 635664) with wash buffer (25 mM MES ...
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bioRxiv - Molecular Biology 2022Quote: ... and amplified by PCR using a forward primer containing a T7 promoter sequence (Supplemental Table 10) with the Advantage HF 2 kit (Takara Bio 639124) and the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated DNA was amplified by PCR using Hot Start TaKaRa LA Taq kit to yield a 10-Kb product (Takara Biotechnology, #RR042A). Primers utilized were the following ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... and resultant RNAs (10 ng per sample) were subjected to cDNA library construction (SMARTer Mouse TCR a/b profiling kit, Takara Bio, Japan). The next generation sequencing was performed with MiSeq (Illumina ...
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bioRxiv - Immunology 2023Quote: ... 110 ng of RNA were retrotranscribed in a final volume of 10 μL using the PrimeScript RT reagent Kit (Takara, Kusatsu, Japan) with a combination of oligo-d(T ...
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bioRxiv - Genetics 2024Quote: ... The reaction was performed in a 20 µl reaction mixtures comprising 10 µl of 2×TB Green® Premix Ex Taq™ (Tli RNaseH Plus) (Cat. no. RR420A, TaKaRa), 1 µl of cDNA ...
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bioRxiv - Genetics 2024Quote: ... The reaction was performed in a 20 µl reaction mixtures comprising 10 µl of 2×TB Green® Premix Ex Taq™ (Tli RNaseH Plus) (Cat#RR420A, TaKaRa), 1 µl of cDNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... concentrated lentivirus was added to the T cells on day 3 at a multiplicity of infection of 10 and subjected to spinoculation in a 24-well plate coated with 30 µg/mL RetroNectin® (Takara, T100B). T cells continued to expand ...
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bioRxiv - Cell Biology 2024Quote: ... The HeLa CCL2 Tet-inducible cell lines were maintained in regular media supplemented with 10% Tet-system-approved FBS (Takara Bio, 631101) and 1% penicillin–streptomycin ...
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bioRxiv - Biophysics 2020Quote: ... was cultured under the following conditions: cells were maintained in DMEM/F-12 50/50 medium containing 10% fetal bovine serum (tetracycline-free, Takara, Mountain View, CA), penicillin (100 units/ml) ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc., Shiga, Japan). Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x ...
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bioRxiv - Physiology 2020Quote: ... containing 1 μL of 10× reaction buffer (SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, Cat. No. 634888, Takara Bio, Kusatsu, Japan). Nuclease-free water was added to 11.5 μL ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Neuroscience 2021Quote: ... anti-nc82 (mouse, 1:10; Developmental Studies Hybridoma Bank, Iowa City, IA, USA) and anti-DsRed (rabbit, 1:500; Takara Bio, Kyoto, JP). Brains were washed three times again in PBS with 0.2% Triton X-100 and transferred into secondary antibody solution (anti-chicken Alexa Fluor 488 ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were carried out in a final volume of 25 μL containing: TAKARA Ex Taq® buffer with MgCl2 (10 X; Takara Bio Inc.), primers (200 nM of each) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were collected at 36 and 72 h and then purified with 10% Lenti-X Concentrator® (Clontech, Mountain View, CA, USA). The virus copy number was determined by Q-PCR ...
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bioRxiv - Bioengineering 2023Quote: ... was synthesized by mixing an oligo DNA (10AA-Fw) containing 10 NNK triplet oligonucleotides and the Lib-Rev primer with dNTPs and Klenow buffer (Takara Bio Inc., Japan) for heating at 95℃ for 3 minutes and cooling at room temperature for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... the input binary libraries (1:10 or 1:100) and the selected pools were subjected to restriction digestion using BamHI (Takara Bio Inc., Japan). The digestion products were then analyzed using a 10% polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μl of DNA isolated through alkaline lysis was used as template in a 10 μl SuperPlex PCR reaction (Takara, catalog number: 638543) with a single primer set at a time (all primers are listed in Supplementary Table S6) ...
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bioRxiv - Neuroscience 2023Quote: ... 45 cycles from 10 s at 95°C to 30s at 60°C using TB Green ® Premix Ex Taq™ GC (Takara Bio, Japan).
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Bioengineering 2023Quote: ... Protease inhibitors and 10% glycerol were added to the supernatant before loading it onto 1 mL of Talon metal affinity resin (Clontech, Mountain View, CA, #635502). After a 20 mL buffer wash ...
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bioRxiv - Genetics 2023Quote: ... The real-time PCR reaction system is as the followings: 10 µL of SYBR Premix Ex Taq™ (Takara Biom edical Technology, Beijing, China), 0.5 µM of each forward or reverses primer and 100-500 ng of DNA template ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 x105 cells/cm2 were immediately added to each well with HEK293 maintenance media modified with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367). After 24 h ...
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bioRxiv - Microbiology 2024Quote: ... the 2nd PCR product was amplified from 10 ng purified 1st PCR product using the TaKaRa Ex Taq HS (TaKaRa Bio Inc., Japan; #RR006A) with a forward (5’-AATGATACGGCGACCACCGAGATCTACAC-Index2-ACACTCTTTCCCTACACGACGC ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from the leaves using the Acid Phenol Guanidium Chloroform method (Chomczynski and Sacchi 2006) and was then treated with 10 U of DNase I (Takara Bio Inc., Kyoto, Japan). poly(A)+ RNA was separated and purified from total RNA using an oligo(dT ...
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bioRxiv - Physiology 2024Quote: ... with 2 ul of RNAse inhibitor mix (containing 0.12 ul Triton X-100 10 %, 0.1 ul RNAse inhibitor (Takara (Cat. No. 2313B, 40 U/ul), 1.78 ul MilliQ water (RNAse-free)) ...
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bioRxiv - Bioengineering 2024Quote: ... A library was independently prepared with 10 ng of total RNA for each sample by SMARTer ultra-low input RNA Sample Prep Kit (Clontech Laboratories, Inc., CA, USA). The first step in the workflow involves purifying the poly-A containing mRNA molecules using poly-T-attached magnetic beads ...
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bioRxiv - Developmental Biology 2024Quote: ... Then the cell lysates were used for reverse transcription and 10-cycle amplification by SMART-Seq HT kit seq (Takara Bio USA, Cat. 634437) following the user manual ...
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bioRxiv - Molecular Biology 2020Quote: A549 cells were obtained from ATCC and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS, Takara Biomedical Technology Co., Ltd., China), 100 U/ml penicillin and 100 U/ml streptomycin (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... RNA was eluted in 10 µl water and immediately proceeded to cDNA generation using the SMART-Seq V4 Ultra Low RNA Kit (Takara Bio USA, Mountain View, CA). 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR reaction was performed in a final volume of 25 µL containing: TAKARA Ex Taq® buffer with MgCl2 (10 X; Takara Bio Inc., Tokyo, Japan), primers 200 nM ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reactions were performed in triplicates in a final volume of 25 µL containing: TAKARA Ex Taq® buffer with MgCl2 (10 X; Takara Bio Inc., Tokyo, Japan), primers 200 nM ...
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bioRxiv - Neuroscience 2024Quote: ... Purified DNA was transfected into nearly confluent (80–90%)10 cm2 plates of low passage LentiX 293T cells (#632180, Takara Bio, San Jose, CA, USA), using a calcium phosphate transfection kit (Takara #631312) ...
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bioRxiv - Microbiology 2024Quote: ... Mycelia were separated from broths via 2 layers of lens paper (10×15 cm, NEWSTAR Industry, Hangzhou, China) and collected for DNA extraction by using DNAiso Reagent (TaKaRa Biotechnology Co. Ltd, Dalian, China) as described in the manual ...
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bioRxiv - Neuroscience 2023Quote: ... We obtained 20 ng/ul RNAs (total 10 ul eluted RNA from one microdissected cell body) that we used for RNAseq (Clontech SMART-Seq Ultra Low Input RNA kit) in Scripps Florida Genomics Core (Currently known as The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology) ...