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Citations for Takara Bio :
601 - 650 of 5029 citations for Rat Hypoxia inducible factor 3 alpha HIF3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Artificially synthesized (G4C2)50 sequences flanked at the 5′ end with an EagI recognition site and at the 3′ end with a PspOMI recognition site were subcloned into T-vector pMD20 (Takara Bio). To generate a longer repeat size ...
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bioRxiv - Molecular Biology 2023Quote: ... Promoterless bicistronic vectors were generated by removing the SV40 promoter from the bicistronic vector pRUF and the pRUF vectors containing the putative IRES sequences by PCR amplification using divergent primers (Supplementary Table 3) and the CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Immunology 2023Quote: ... Lentivirus-containing supernatant was harvested on days 2 and 3 after transfection and then concentrated using a Lenti-X concentrator (Clontech, 631232). HEK-293T cells were transfected with the expression vectors according to the manufacturer’s protocol with PEI 2500 (BioScientific) ...
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bioRxiv - Cell Biology 2023Quote: ... the University of North Carolina at Chapel Hill) (43) and inserted back into pIZ-Msps-GFP digested with SspI(5’) and XhoI(3’) using Infusion ligation (Takara Bio) to create pIZ-Msps (RNAi-resistant)-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purification of Ypq1-3C-GFP-8xHis was done by incubating the supernatant with 3 mL TALON cobalt resin (635502; TaKaRa Bio) pre-equilibrated with Column buffer (20 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting PCR product was cloned between the 3’ and 5’ arms of the Rosa26 targeting vector using In-Fusion cloning (Takara Bio). Plasmid sequence was checked by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... and of human FAM104B isoform 3 (NM_001166700.2) were PCR- amplified from a yeast two-hybrid human testis cDNA library (Clontech, cat. no. 638810) and cloned via appropriate restriction sites into pGAD-C1 (James et al ...
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bioRxiv - Neuroscience 2023Quote: ... the SV40pA was replaced by the 3’UTR of msSOD2 (Kaltimbacher et al., 2006) amplified from mouse brain cDNA (Clontech 637301) using primers AA35 and AA36 and inserted into the XbaI and NotI sites ...
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bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated p53 KO cell lines were electroporated with 3 µg p53 firefly luciferase reporter plasmid pp53-TA-Luc (Clontech/Takara) and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe Reverse Transcriptase (Takara ClonTech #639538) as described92 ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Genomics 2022Quote: ... Paris) were prepared following the kit manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit from Takara). Samples were sequenced on a NextSeq 500 Illumina device with MidOutPut cartridge to generate a total of 2 × 87 M reads of 75 bp ...
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bioRxiv - Genetics 2021Quote: ... The qPCR procedure was carried out according to the kit instructions (TB Green Premix Ex Taq kit, TakaRa). Primers used were as follows:
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bioRxiv - Immunology 2020Quote: ... and reverse transcribed using a first-strand cDNA synthesis kit (PrimeScript™ 1st strand cDNA Synthesis Kit, Takara) according to the manufacturer’s protocol ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit, Takara, Shiga, Japan). SDS-PAGE and Western blotting were performed as previously described (Shintani et al. ...
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bioRxiv - Bioengineering 2022Quote: ... prepared with a SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian kit (Takara Bio Inc., Japan) with 0.75 ng and bulk-sequenced using 100 bp paired-end reads at a depth of 50 million reads ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2-Pico Input Mammalian kit (Takara). Two technical replicates were generated for each experiment ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript RT reagent kit (Perfect Real Time Kit, Takara Biochemicals, China). According to the vendor’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Libraries were constructed with the SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian kit (Clontech Laboratories), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian kit (Takara) due to retirement of the ScriptSeq v2 kit ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser Kit (TaKaRa, Japan) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The SMARTer Pico RNA V2 Kit (Clontech) was used for library preparation ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription kit was purchased from TaKaRa, Japan.SYBR Green PCR kit was purchased from American Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... using the Reverse Transcriptase Kit (Takara, Japan). Quantitative PCR reactions were carried out in duplicate ...
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bioRxiv - Neuroscience 2022Quote: ... Using the In Fusion Cloning Kit (Clontech) the 5,000 bp amplicon carrying the mutation was inserted into the full-length Reelin-coding plasmid digested with SnaBI and AgeI.
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bioRxiv - Plant Biology 2020Quote: ... using a commercial kit (TaKaRa, Shiga, Japan). Before applying to the root segments ...
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bioRxiv - Cancer Biology 2019Quote: ... purified with NucleoBond Xtra Midi kit (Clontech), and sequenced (Genewiz).
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bioRxiv - Immunology 2019Quote: LDH Cytotoxicity Detection kit (MK401, Takara Bio) was used as per manufacturer instructions to detect cell killing.
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bioRxiv - Molecular Biology 2020Quote: ... Premix Taq version 2.0 kit (TaKaRa, Japan) was used for PCR reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... The Advantage GC 2 PCR Kit (Takara) was used for gene cloning ...
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bioRxiv - Physiology 2019Quote: ... the NucleoSpin RNA XS kit (Takara Bio) was used ...
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bioRxiv - Bioengineering 2019Quote: ... using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to the target sequence on M ...
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bioRxiv - Bioengineering 2019Quote: ... using the Advantage 2 Polymerase kit (Clontech) and specific primers located on either side of the target locus ...
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bioRxiv - Genomics 2021Quote: ... SMART-Seq RNA Kit (v4) (Takara, 634891), TruSeq Library Prep (Illumina ...
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bioRxiv - Genomics 2021Quote: ... SMART-Seq Single Cell Kit (Takara Bio) libraries were prepared following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PrimeScript cDNA synthesis kits (Takara Bio) were used to convert 1 μg of total RNAs into cDNA according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... using the In-Fusion HD kit (Takara), followed by transformations into MegaX DH10B T1R electrocompetent cells (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Takara Prime Script™ RT kits (Takara Bio Inc. ...
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bioRxiv - Biophysics 2021Quote: ... using In-Fusion HD Cloning Kit (Clontech). BP84 is a chimeric protein of 1-83 amino acid sequence of Bacillus ...
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bioRxiv - Neuroscience 2021Quote: ... and In-Fusion HD Cloning kit (Clontech), respectively ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using NucleoBond Xtra midiprep kits (Takara Bio).
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bioRxiv - Plant Biology 2020Quote: ... using DNA Ligation Kit
(Takara). The constructed vector was transformed into the DH5α strain of E.coli ... -
bioRxiv - Plant Biology 2021Quote: ... An SYBR Premix Ex Taq kit (Takara) was used to quantitative real-time RT-PCR ...
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bioRxiv - Immunology 2021Quote: ... The PrimeScript RT Master Mix Kit (Takara) was used for cDNA generation ...
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bioRxiv - Molecular Biology 2021Quote: ... and DNA Unique Dual Index Kit (Takara). The multiplexed libraries were dispatched to Eurofins Genomics Inc ...
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bioRxiv - Neuroscience 2021Quote: ... The ribo-depletion kit (RiboGone, Takara, CA) and SMARTer Stranded total RNA sample prep kit were used to remove excess ribosomal RNA and synthesize cDNA library ...
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bioRxiv - Microbiology 2021Quote: ... by using the DNA ligation Kit (Takara). Plasmid DNA was then linearized with the restriction enzyme XhoI ...
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bioRxiv - Plant Biology 2021Quote: ... or In-Fusion HD-Cloning kit (Clontech). For inserting a gRNA fragment ...