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Citations for Takara Bio :
551 - 600 of 3733 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The HBV core protein coding region was amplified using the forward primer 5’-ATCATAAGCTTACCATGGACATCGACCCTTATAAAG-3’ and reverse primer 5’-TAGATGGTACCCTAACATTGAGGTTCCCGAG-3’ and subcloned into the pcDNA3.1 vector (Clontech) via HindIII and KpnI restriction sites ...
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bioRxiv - Genetics 2020Quote: ... and reverse transcription (RT) was performed using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA Bio, Kusatsu, Japan). Primers were designed to specifically amplify cDNA from transfected vectors and endogenous RPGR from 293T and ARPE-19 cells respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified by RT-PCR and cloned into pMSCV-IRES-eGFP using In-Fusion® HD Cloning Kit User Manual (Takara, Cat#638920, Shiga, Japan) following the manufacturer’s instructions (see Table 3 for primer sequences).
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bioRxiv - Molecular Biology 2022Quote: ... PCR1 (10 cycles of amplification) was performed with the addition of SMARTer RNA Unique Dual Index Set A and Set B (Takara SMARTer RNA Unique Dual Index Kit-96A). Samples were pooled into quadrants and depletion of ribosomal cDNA was performed ...
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bioRxiv - Plant Biology 2020Quote: ... Takara Prime Script™ RT kits (Takara Bio Inc. ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR mix (Takara Bio Inc., Japan), with primers and probe specific for the SARS-CoV-2 E gene ...
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bioRxiv - Immunology 2021Quote: ... The PrimeScript RT Master Mix Kit (Takara) was used for cDNA generation ...
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bioRxiv - Developmental Biology 2022Quote: ... Prime Script RT Master Mix (Takara, RR036A) was used to reverse transcribe 1 µg of total RNA into cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... The RT kit was obtained from Takara Bio USA (Moutain view ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 μL PrimeScript RT (Takara, USA), and the reaction mix was incubated at 30 °C for 10 minutes ...
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bioRxiv - Pathology 2020Quote: ... The RT kit was obtained from Takara Bio USA (Mountain View ...
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bioRxiv - Microbiology 2021Quote: ... PrimeScript RT Master Mix (TaKaRa, Cat# RR036A) were used to generate complementary DNAs (cDNAs ...
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bioRxiv - Immunology 2021Quote: ... and PrimeScript RT Reagent Kit (Takara, RR037A). Forward and reverse primers were designed to target SRBD (Forward ...
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bioRxiv - Neuroscience 2020Quote: ... and PrimeScript RT reagent kit (RR037, Takara), respectively ...
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bioRxiv - Plant Biology 2022Quote: ... 5 × PrimeScript™ RT Master Mix (TAKARA) was used to synthesize cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... PrimeScript™ RT reagent kit (TaKaRa, RR036A) is used to generate cDNA from RNA extracted ...
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bioRxiv - Immunology 2023Quote: ... or PrimeScript RT reagent Kit (Takara, RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2023Quote: ... or the PrimeScript RT Master Mix (Takara). In each individual experiment ...
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bioRxiv - Plant Biology 2024Quote: ... using the PrimeScript RT Master Mix (Takara). We then amplified cDNA in Power SYBR (Thermo ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we performed Rapid-Amplification-of-cDNA-Ends (RACE) PCR using transcript-specific primers and the SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc., Mountain View, CA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... Specific forward and reverse primers (Suppl Table 3) were designed using the In-Fusion Cloning Primer Design Tool from TaKaRa (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools ...
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bioRxiv - Molecular Biology 2019Quote: Three specific 5’ RACE primers and two 3’ RACE primers were designed according to the Race kit instructions (Invitrogen & Clontech) (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... 226 or 464 site mutation forward and reverse primers and 20 μM each of JEV-NS3 forward and reverse primers using PrimeSTAR GXL DNA Polymerase (TAKARA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was annealed to primers by the addition of 4 µL 100 µM Random Hexamer Primers (TaKaRa, Mountain View, CA) and incubation at 65°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reaction was prepared using gene-specific primers (20µM, Eurofins, primer sequences in Suppl. Table 4) and PrimeSTAR® Max DNA Polymerase (Takara) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Reverse transcription (RT) was performed using a PrimeScript® RT Reagent Kit with gDNA Eraser (RR047A, TaKaRa, Dalian, China) in a 20 μL reaction mixture containing 1 μg of total RNA from each individual sample ...
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bioRxiv - Immunology 2021Quote: ... the commercial 10x universal primer mix (Takara USA) was used at a final 1x concentration ...
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bioRxiv - Immunology 2021Quote: ... The commercial 10x universal primer mix (Takara USA) was used at a final 1x concentration ...
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bioRxiv - Immunology 2022Quote: ... Primer/Probe N2 (2019-nCoV) (Takara, Cat# XD0008) were used as follows ...
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bioRxiv - Microbiology 2020Quote: ... prepared using a random primer labeling kit (Takara)79.
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bioRxiv - Microbiology 2023Quote: ... we used Primer/Probe N2 (2019-nCoV) (TaKaRa). The reaction conditions for RT-PCR were 42 °C for 5 min and 95 °C for 10 s ...
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bioRxiv - Cell Biology 2023Quote: ... The primers for In-fusion cloning (Takara Bio) Fstl1 are (Forward 5’-CACAGACGCGTACCGGTGCCACCATGTGGAAACGATGGCTGGCGCTC-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... using oligo-dT primer (#3805, Takara, Shiga, Japan). A quantitative (q)PCR was performed on a Thermal Cycler Dice Real Time System (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... protocol with the P5 forward primer mix (Takara) and a unique reverse P7 primer for each condition ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to RT tubes (Maxima RT: Life Technologies, Inc., EP0753; 10mM dNTP Life Technologies, Inc., 4303443; RNAase inhibitor: Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA was subjected to reverse transcription (RT) for 15 min at 37°C with the use of a PrimeScript RT Reagent Kit with gDNA Eraser (Takara), after which the reaction was terminated by incubation at 85°C for 5 s ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... a Prime Script RT kit (Takara, Tokyo, Japan) was adopted for the generation of complementary DNA ...
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bioRxiv - Cell Biology 2022Quote: ... Using the PrimeScript RT Master Mix (Takara Bio), we reverse transcribed total RNA ...
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bioRxiv - Immunology 2021Quote: ... and the PrimeScriptTM RT reagent Kit (TaKaRa, Japan) as described in previous studies (Li et al. ...
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bioRxiv - Genomics 2019Quote: ... RNA was reverse transcribed using Smartscribe RT (Clontech) and ISPCR-OligodT primer ISPCR-TSO (Table S1) ...
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bioRxiv - Cell Biology 2021Quote: ... A PrimeScript RT reagent kit (TaKaRa, Seoul, Korea) was used to reverse-transcribe mRNA into cDNA ...
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bioRxiv - Microbiology 2021Quote: ... using a PrimeScript RT Reagent Kit (Takara Bio) in a 10 mL reaction ...
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bioRxiv - Cell Biology 2021Quote: ... The PrimeScript™RT reagent Kit (Takara, Japan) was used to synthesize lncRNA-UCA1 cDNA and the AceQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Microbiology 2022Quote: ... The PrimeScript™ RT Reagent Kit (TaKaRa, Japan) was used to reverse-transcribe cDNA from total RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Prime Script RT reagent Kit (Takara, RR047A, Japan) were used to reverse transcribe the extracted RNA into cDNA according to the given instructions ...
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bioRxiv - Bioengineering 2021Quote: ... cDNA using PrimeScript™ RT Master Mix (Takara) and quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2019Quote: ... synthesized using the PrimeScript RT regent Kit (TaKARa), or isolated gDNA was the subsequent template ...