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Citations for Takara Bio :
551 - 575 of 575 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and Foxa3 were obtained by reverse transcription PCR using a template RNA extracted from a C57BL/6J liver using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TAKARA Bio, Japan). The coding sequences of the primers used are listed in Table S3 ...
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bioRxiv - Microbiology 2020Quote: ... SYBRgreen real-time quantitative assays were made by using the SYBR® Premix Ex Taq™ II (Tli RNaseH Plus; Takara Bion Inc.) and an Applied Biosystems® Step One PCR System ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from total RNA of each culture sample using the PrimeScript II 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan) with random hexamers to yield a total volume of 20 μL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Quantitative PCR (qPCR) reactions were prepared in triplicate using TB Green® Premix Ex Taq™ II (Tli RNase H Plus) (Takara, USA) with a modified protocol ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from LNs and used for RT-qPCR using a Prime Script™ II Strand cDNA Synthesis kit (Takara, Dalian, China), in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Quantitative RT-PCR (qPCR) for target genes was performed using TB Green®Premix Ex Taq™ II (Tli RNaseH Plus) (Takara, Japan) and using gene-specific primers listed in Table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was then subjected to the subsequent PCR using TB Green Premix Ex™ Taq II (Tli RNaseH Plus) (#RR820A; TaKaRa, Kusatsu, Japan) on a Thermal Cycler Dice Real Time System II MRQ (TP960 ...
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bioRxiv - Developmental Biology 2024Quote: ... were individually re-amplified using the 5’ PCR Primer II A with CloneAmp HiFi PCR Premix (Clontech Laboratories, Inc. A Takara Bio Company, #639298) by heating to 98°C x 2 min followed by 20 cycles of 98°C x 10 s ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA (100–300 ng) extracted from sex-determined mature embryos was reverse-transcribed using PrimeScript™ II Reverse Transcriptase (TaKaRa, Shiga, Japan) at 30°C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Diluted cDNA was used as a template for qPCR using a TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA was then used to quantify the expression levels of the cytokines by using TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara Bio Inc., Japan) TNF-α ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using the TB GreenTM Premix Ex TaqTM II (Takara Bio Inc., Kusatsu, Japan) according to the manufacturer’s protocol for a total reaction volume of 20 μl ...
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bioRxiv - Developmental Biology 2020Quote: ... Expression levels of the stag paralogues (primer sequences in Supplementary Table S3) and haematopoietic markers were measured using SYBR Premix Ex Taq II (Takara Bio Inc., Kusatsu, Japan) on a Roche LightCycler400 ...
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bioRxiv - Biochemistry 2021Quote: ... (ii) addition of a second adapter on the 3’ end of the cDNA during reverse transcription using SmartScribe RT (Clontech Biotechnologies, Mountain View, CA) as previously described (63) ...
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bioRxiv - Systems Biology 2021Quote: ... We prepared ligation-free ribosome profiling and total RNA-seq libraries from the clarified polysome lysates treated for 6 hours following the instructions provided with their respective kits (smarter-seq smRNA-seq kit, Takara-Clontech; NEBnext Ultra-Directional II) augmented with our previously-published ligation-free ribosome profiling protocol42 ...
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bioRxiv - Plant Biology 2021Quote: ... after which it was used as the template for synthesizing cDNA using the PrimeScript™ II 1st strand cDNA Synthesis Kit (Takara Bio, Beijing, China). The full-length ZmCd1 cDNA was amplified by PCR using Phanta Max Super-Fidelity DNA Polymerase (Vazyme Biotech) ...
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bioRxiv - Microbiology 2021Quote: ... Real time PCR (qPCR) was carried out in triplicates for each sample using TB Green® Premix Ex Taq™ II (TaKaRa, Dalian, China) in The CFX96 touch Real-Time PCR System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting GST-S-LacI fusion sequence was then amplified by PCR using primers eo1211 and eo1212 (Supplementary Table S1) and inserted between the NdeI and HindIII sites in the pCold II vector (Takara Bio, Shiga, Japan, #3362) by the In-Fusion reaction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The relative expression of mRNA and miRNA was respectively quantified using TB Green® PremixExTaq™ II (Tli RNaseH Plus) (Cat# RR820A, Takara, Kyoto, Japan) and miRcute Plus miRNA qPCR Detection Kit (Cat# FP411 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Each cDNA sample was amplified for the gene of interest and β-actin in a 15 µL reaction volume TB GreenTM Premix Ex Taq™ II (Takara, Tokyo, Japan). The PCR conditions were 95 °C for 30 s followed by 40 cycles of 95 °C for 5 s and 60 °C for 60 s ...
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bioRxiv - Genetics 2024Quote: ... mRNA expression was quantified by quantitative real-time PCR (qPCR) using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara Bio, Japan) on a Thermal Cycler Dice Real-Time PCR System (Takara Bio ...
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bioRxiv - Systems Biology 2021Quote: ... We prepared ligation-free ribosome profiling and total RNA-seq libraries from the clarified polysome lysates treated for 6 hours following the instructions provided with their respective kits (smarter-seq smRNA-seq kit, Takara-Clontech; NEBnext Ultra-Directional II) augmented with our previously-published ligation-free ribosome profiling protocol42 ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified and analysed in real-time polymerase chain reaction on a Roche Light Cycler 480 system with TB Green® Premix Ex Taq™ II (TaKaRa Bio Inc., Japan). The specific primers were predesigned and validated by PrimerBank of Massachusetts General Hospital (Spandidos et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... The converted cDNA was amplified using gene-specific primers and TB Green® Premix Ex Taq™ II (Cat# RR820A; DSS Takara Bio India Private Ltd) to quantify the expression of the genes using a real-time PCR system (Applied Biosystem Quant Studio 7 Flex ...