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Citations for Takara Bio :
451 - 500 of 575 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... qPCR was conducted with 10ng genomic DNA in duplicate in a 20 μl reaction using the TB Green Premix Ex Taq II (Tli RNase H Plus, Takara) and the StepOnePlus™ Real-Time PCR System (ABI) ...
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bioRxiv - Microbiology 2023Quote: ... and were quantified by real-time RT-PCR using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... and subjected to quantitative real-time polymerase chain reaction with a TB Green Premix Ex Taq II kit (Takara, RR820A). The PCR program consisted of 95°C for 1 minute ...
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bioRxiv - Molecular Biology 2024Quote: ... The quantitative real-time-polymerase chain reaction (qPCR) was conducted with SYBR Premix Ex Taq™ II (Takara, Dalian, China), and performed on on a QuantStudio™ 7 Flex Real□ Time PCR System ...
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bioRxiv - Microbiology 2024Quote: ... cDNA levels of the phoP and eptB genes were quantified by PCR using SYBR Premix Ex Taq II (Takara, Japan) solution containing RT-PCR primers for each gene (See Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice and single PBs (CD138+ CD38lo CD19+/lo CD3- live singlet lymphocytes) were sorted using a FACSAria II into 96-well plates containing 2 µL Lysis Buffer (Clontech) supplemented with 1 U/µL rNase inhibitor (NEB ...
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bioRxiv - Immunology 2024Quote: ... and used as a template for qPCR with TB Green® Premix Ex TaqTM II (Tli TNaseJ Plus) (Takara, #RR820L) and the following primers (Eurofins):
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were performed using TB Green Premix Ex Taq TM II (Tli RNaseH Plus; Takara) on a Light Cycler 480 II (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative genomic real-time PCR (gRT-PCR) reaction was performed using TB Green Premix Ex Taq TM II (Tli RNaseH Plus; Takara) following standard procedures ...
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bioRxiv - Bioengineering 2024Quote: ... the RT-PCR reaction was performed in 20 μL of reaction buffer containing 10 μL of TB green Premix Ex Taq II (RR82WR; Takara), 1 μL of 10 μM forward primer ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5 LR cells) were individually re-amplified using the 5’ PCR Primer II A with CloneAmp HiFi PCR Premix (Clontech Laboratories ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA (100–300 ng) extracted from pooled male or female mature embryos (108 hpo) was reverse transcribed using PrimeScript II reverse transcriptase (TaKaRa) as described above ...
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bioRxiv - Plant Biology 2024Quote: ... and one of 15 µl purified DNA was used for real-time PCR amplification using SYBR Premix Ex Taq II (Tli RnaseH Plus) (TaKaRa) and Real-Time LightCycler 480 system (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... in a final volume of 20 µl and one microlitre of cDNA was used for subsequent amplification using SYBR Premix Ex Taq II (Tli RnaseH Plus) (TaKaRa) and Real-Time LightCycler 480 system (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... following the manufacturer’s instructions and were reverse transcribed into cDNA using the PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara). RT‒qPCR analysis of bcd cDNA and its targets was performed using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: 0,1 ng of retrieved DNA in Cut&Run were used as template in a real time quantitative PCR assay using SYBR® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qPCR reactions were performed in duplicates using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Immunology 2021Quote: ... the isolated RNAs were subjected to first-strand cDNA synthesis using (5’-GTCGTATCCAGTGCAGGGTCCGAGGTCACTG GATACGACATACAACA-3’) by PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, Japan). After that ...
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Mis6/CENP-I maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosisbioRxiv - Cell Biology 2021Quote: ... the rabbit anti-RNA polymerase II (phosphoS5) polyclonal antibody (1:100; ab5131) or rabbit anti-GFP polyclonal antibody (1:250; Clontech, 632592) was incubated with 200 µL of the lysate for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis was performed using TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio) on a Thermal Cycler Dice Real Time System TP800 (Takara Bio) ...
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bioRxiv - Plant Biology 2020Quote: ... The LightCycler 480II PCR system was programmed according to the instructions of the SYBR Premix Ex Taq II (Perfect Real Time) kit (TaKaRa, China) to conduct fluorescence-based quantitative PCR ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting cDNA templates were subject to qRT-PCR analyses using TB Green® Premix Ex Taq™ II (RR820Q, Takara) with a StepOneTM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed on 1 μL of 1 in 40 diluted cDNA in 15 μL reactions using SYBR Premix Ex-Taq II reagent (Takara Bio) in a Bio-Rad CFX96 real-time system (see Table S1 for primer pairs) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was used as a template for SYBR Green-based qPCR using TB Green Premix Ex Taq™ II Reagent (Clontech) and an ABI Quant 5 thermocycler (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from total RNA (0.5 μg) using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa, Japan). PCR was conducted with TaKaRa LA Taq® with GC buffer (TaKaRa ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate using SYBR Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa), Fast SYBR Green Master Mix (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II, Clontech).
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified and ligated into the pET15b vector with a C-terminal His tag to create plasmid pET-AdpA by using the ClonExpress™ II One Step Cloning Kit (TaKaRa). The plasmid was transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... Differential regulation of cellular genes was assessed using TB Green™ Premix Ex Taq™ II (Tli RNase H Plus; Takara) according to manufacturer’s instructions with QuantiTect primer assays (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time RT-PCR analysis was performed using the Thermal Cycler Dice Real-Time System II (Takara Bio Inc., Shiga, Japan) to detect the mRNA expression of GPX4 ...
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bioRxiv - Microbiology 2020Quote: ... Construction of strains with cytoplasmic sGFP expression was performed by cloning the sGFP expression cassette from plasmid pIGPAPA and the neo cassette from plasmid pSD1 to the polylinker of plasmid pBluescript II (using the T4 DNA ligase, Takara Bio). The resulting plasmid pBS-GFP-gen was used to transform V ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR assay was performed using 1/20 diluted cDNA as templates in the reactions containing SYBR® Premix Ex TaqTM II (TaKaRa). The qRT-PCR assay was conducted in triplicate in an ABI 7500 Fast Real-Time PCR System ...
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bioRxiv - Genomics 2020Quote: ... The cDNA synthesis was carried out by using 5 g of total RNA or 1 g of PAPed RNA with RT primer (5-TTTTTTTTUUUTTTTTVN-3) by PrimeScript II Reverse Transcriptase (TaKaRa Bio). The full-length cDNAs were selected by Cap Trapper method 60 ...
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bioRxiv - Microbiology 2020Quote: ... QRT-PCR reaction in 20ul reactions containing 10ul TB Green Premix Ex Taq II(Tli RNaseH Plus)(TaKaRa, made in china), 3µl of cDNA and 0.4uM forward primers and 0.4uM reverse primers ...
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bioRxiv - Immunology 2021Quote: ... First strand cDNA synthesis was performed with 4 μg of total RNA per reaction using PrimeScript™ II 1st Strand cDNA Synthesis Kit and oligo-dT primer (TAKARA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
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bioRxiv - Neuroscience 2021Quote: The desired ORF for Pdlim7 (Gene bank ID: AF345904.1) was amplified by PCR from Human Universal QUICK-Clone™ II (Takara 637260) using primers depicted in Table S2 and inserted into pEGFP-C1 plasmid through Gibson assembly.
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bioRxiv - Microbiology 2020Quote: ... then subjected to quantitative real-time RT-PCR using a One-Step SYBR PrimeScript RT-PCR kit II (Takara Bio, Japan). The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38 ...
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bioRxiv - Microbiology 2021Quote: ... First the coding region of Candid #1 RdRp was amplified using RNA extracted from P0 or P11 viruses and the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio). Kozak sequence ...
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bioRxiv - Neuroscience 2023Quote: ... and the expression of specific gene was analysed using TB Green® Premix Ex Taq™ II (TaKaRa Bio Inc., Japan) in a Roche Light Cycler 480 with predesigned and validated primers from PrimerBank of Massachusetts General Hospital (61) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Bcl-2 and PTEN were detected by RT-PCR with TB Green TM Premix Ex TaqTM II(Tli RNaseH Plus) (RR820A, Takara, Japan). The complete gene sequences showed in Table 2 were searched from the National Center for Biotechnology Information (NCBI ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting cDNA was utilized for analysis using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara Bio). Quantitative PCR was performed using Applied Biosystems QuantStudio™ 5 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed using TB Green Premix Ex Taq ™ II (Tli RNaseH Plus) (Takara) with qTOWER3 (Jena) ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed with GeneAce SYBR qPCR Mix α No ROX (Nippon Gene, Tokyo, Japan) in Thermal Cycler Dice Real Time System II (Takara). Each Actin1 genes (PtActin1 and TpActin1 ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR reactions were implemented in an Applied Biosystems 7500 Real-Time PCR system with the SYBR® Premix Ex TaqTM II (TAKARA). ACTIN was used as the reference gene for qPCR analyses ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara, RR820B) on a Real-Time PCR detection system (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extraction and reverse transcription of RNA into cDNA were performed based on the method described as manufacturer’s protocol of Prime Script TMΠ 1stStrand cDNA Synthesis Kit andTB GreenTM Premix Ex TaqTM II (TaKaRa, Beijing, China). qRT-PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Cell Biology 2022Quote: ... NM_001123007) was amplified from 24 hpf embryo RNA using the PrimeScript II High Fidelity One Step RT-PCR kit (Takara, R026A) and the following primers ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan). qRT-PCR was performed with Roche LightCycler 480 Real Time PCR system (Roche ...