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Citations for Takara Bio :
501 - 550 of 5220 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... or PrimeSTAR Mutagenesis Basal Kit (TaKaRa). We used E ...
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bioRxiv - Cell Biology 2024Quote: ... A qRT-PCR kit (TaKaRa, Japan) was used to measure the expression levels of PCBP1 ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of produced viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Genetics 2024Quote: Transcription Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and PrimeScriptTM RT reagent kit (Takara). RT-qPCR was performed using QuantiNova Probe PCR Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... using In-Fusion cloning kit (Takara). Truncated versions of FLAG::TurboID::SQSTM1 were generated by PCR using primers corresponding to the truncated SQSTM1 gene and amplification using Q5 high-fidelity (M0492 ...
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bioRxiv - Molecular Biology 2024Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT- qPCR using PowerUp SYBR-Green MasterMix (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... In-Fusion HD Cloning Kits (Takara) were used in Ligation Independent Cloning (LIC ...
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bioRxiv - Cancer Biology 2024Quote: An RNA extraction kit (Takara Kusatsu) was used for RNA extraction according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... In-Fusion HD cloning kit (Takara) was used to insert the fragments into the desired linearized vector featuring a GS linker-fluorescent tag fusion ...
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bioRxiv - Cancer Biology 2020Quote: ... and TRIM27 cDNA was obtained in pENTR221 vectors from the UT Southwestern (UTSW) McDermott Center for Human Genetics and subcloned into pCMV-HA or pCMV-myc (Clontech, Mountain View, CA) between SalI and NotI restriction sites ...
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bioRxiv - Immunology 2022Quote: ... Viruses were packaged in human embryonic kidney (HEK) 293 cells and viral supernatants were processed using Retro-X concentrators (Takara Bio. USA Inc). Naïve CD8 T cells were purified by negative-selection and stimulated in vitro with plate-bound anti- CD3/CD28 ...
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bioRxiv - Biochemistry 2021Quote: A cDNA fragment encoding full-length human EGFR (UniProt accession no. P00533) was cloned into the pEGFP-N1 plasmid (Clontech, Mountain View, CA). To facilitate affinity purification ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.25Lμg pVSV-G (second-generation lentivirus packaging system) into human embryonic kidney packaging cells GP2-293 (Clontech, Inc., Mountain View, CA, USA), using CalPhos™ Mammalian Transfection Kit (Clontech ...
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bioRxiv - Cell Biology 2022Quote: Mouse NIH/3T3 (ATCC, cat# CRL-1658), human IMR-90 (ATCC, cat# CCL-186) and Lenti-X™ 293T (Takara Bio, cat# 632180) cell lines were cultured in full-DMEM (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... A bicistronic construct expressing human EPAC1b with a C-terminal His10 tag and SUMO3(Q89K) was constructed using the pIRES2-EGFP vector (Clontech Catalog no. 632435). The EPAC1-His10-IRES-SUMO3(Q89K ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic stem cell (hESC)-derived cerebral organoids (hCOs) were generated from a commercially available hESC stem cell line (Takara Bio, Osaka, Japan), using the STEMdiff cerebral organoid kit (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... expression plasmid was constructed by cloning the complementary DNA (cDNA) of human TRF2 with an N-terminal myc-tag into the pLVX-tetOnePuro plasmid (Takara Bio, Kusatsu, Japan). The lentiviral human BCAT2 expression plasmid using a pLVXneo backbone was designed and ordered from VectorBuilder (Chicago ...
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bioRxiv - Neuroscience 2020Quote: ... and reverse transcribed using complementary DNA (cDNA) synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio). The synthesized cDNA was subjected to qPCR using KAPA SYBR (KAPABiosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined using a qPCR Lentivirus Titration Kit (Lenti-X, qRT-PCR Titration Kit, Takara). For smaller scale virus preparation ...
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bioRxiv - Neuroscience 2020Quote: ... in a 100 mm dish using a calcium phosphate transfection kit (CalPhos Mammalian Transfection kit, Clontech, 631312). Two days post-transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... The kit used for qPCR experiments was PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan), HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme Biotech Co. ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the ThruPLEX DNA-Seq Kit and DNA Unique Dual Index Kit (Takara) according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2022Quote: ... Constructs for human Tara were prepared by cloning full-length TRIOBP1 (Trio and F-actin binding protein1) isoform into pEGFP-C3 (Clontech, Mountain View, CA, USA), pFLAG-CMV2 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... which is an abundant splice isoform in human brain26 was engineered in the mammalian expression vector pIRES2-EGFP (BD Biosciences-Clontech, Mountain View, CA, USA). The EGFP-expressing vector was used for transfection of WT or variant KCNQ2 into CHO-Q3 cells (homozygous state) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were subcloned into pCS2-3×Flag (Wang et al., 2017) or pmCherry-N1 and CDSs encoding CASP (pufferfish, human and mouse) were subcloned into p-CMV-Myc (Clontech, Mountain View, CA, USA) or pCS2-Myc (Wang et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... the structural homologue regions from human and gecko EVX1AS were in vitro transcribed (IVT) using a T7 RNA Polymerase (Takara Bio, San Jose, CA). IVT lncRNAs were then purified ...
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bioRxiv - Genomics 2022Quote: ... Paris) were prepared following the kit manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit from Takara). Samples were sequenced on a NextSeq 500 Illumina device with MidOutPut cartridge to generate a total of 2 × 87 M reads of 75 bp ...
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bioRxiv - Genetics 2021Quote: ... The qPCR procedure was carried out according to the kit instructions (TB Green Premix Ex Taq kit, TakaRa). Primers used were as follows:
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bioRxiv - Immunology 2020Quote: ... and reverse transcribed using a first-strand cDNA synthesis kit (PrimeScript™ 1st strand cDNA Synthesis Kit, Takara) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Libraries were constructed with the SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian kit (Clontech Laboratories), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser Kit (TaKaRa, Japan) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit, Takara, Shiga, Japan). SDS-PAGE and Western blotting were performed as previously described (Shintani et al. ...
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bioRxiv - Bioengineering 2022Quote: ... prepared with a SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian kit (Takara Bio Inc., Japan) with 0.75 ng and bulk-sequenced using 100 bp paired-end reads at a depth of 50 million reads ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2-Pico Input Mammalian kit (Takara). Two technical replicates were generated for each experiment ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript RT reagent kit (Perfect Real Time Kit, Takara Biochemicals, China). According to the vendor’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The SMARTer Pico RNA V2 Kit (Clontech) was used for library preparation ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription kit was purchased from TaKaRa, Japan.SYBR Green PCR kit was purchased from American Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... using the Reverse Transcriptase Kit (Takara, Japan). Quantitative PCR reactions were carried out in duplicate ...
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bioRxiv - Neuroscience 2022Quote: ... Using the In Fusion Cloning Kit (Clontech) the 5,000 bp amplicon carrying the mutation was inserted into the full-length Reelin-coding plasmid digested with SnaBI and AgeI.
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bioRxiv - Plant Biology 2020Quote: ... using a commercial kit (TaKaRa, Shiga, Japan). Before applying to the root segments ...
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bioRxiv - Molecular Biology 2020Quote: ... Premix Taq version 2.0 kit (TaKaRa, Japan) was used for PCR reaction ...
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bioRxiv - Genomics 2021Quote: ... SMART-Seq RNA Kit (v4) (Takara, 634891), TruSeq Library Prep (Illumina ...
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bioRxiv - Genomics 2021Quote: ... SMART-Seq Single Cell Kit (Takara Bio) libraries were prepared following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PrimeScript cDNA synthesis kits (Takara Bio) were used to convert 1 μg of total RNAs into cDNA according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... using the In-Fusion HD kit (Takara), followed by transformations into MegaX DH10B T1R electrocompetent cells (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Takara Prime Script™ RT kits (Takara Bio Inc. ...
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bioRxiv - Biophysics 2021Quote: ... using In-Fusion HD Cloning Kit (Clontech). BP84 is a chimeric protein of 1-83 amino acid sequence of Bacillus ...