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Citations for Takara Bio :
451 - 500 of 5220 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: The desired ORF for Pdlim7 (Gene bank ID: AF345904.1) was amplified by PCR from Human Universal QUICK-Clone™ II (Takara 637260) using primers depicted in Table S2 and inserted into pEGFP-C1 plasmid through Gibson assembly.
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: The shRNA target sequence for human HSF1 (NM_005526.4) was selected using the RNAi Target Sequence Selector (Clontech, Mountain View, CA, USA). The target sequences were ...
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bioRxiv - Microbiology 2021Quote: ... Phage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells, PB003F and PB003C) and total RNA from PBMCs (TaKaRa, 636592), spleens (TaKaRa ...
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bioRxiv - Biochemistry 2023Quote: Mouse UCP1 gene and human codon-optimized mitoLbNOX and LbNOX (addgene #74448 and #75285) were cloned into pLVX-TRE3G vector (Clontech, CA) using Gibson assembly ...
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bioRxiv - Cell Biology 2023Quote: The plasmid pEGFP-N1–VMP1 was obtained by inserting the human VMP1 sequence (NCBI reference sequence: NM_030938.5) into pEGFP-N1 (CLONTECH cat. #6085-1). The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5 ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of EPHX2 was amplified by PCR from liver cDNA (Human Multiple Tissue cDNA panels; Takara Bio, Shiga, Japan) using primers (5’-GTCGACATGACGCTGCGCGCGGCCGTCTTCG-3’ ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR for pluripotency and trilineage spontaneous differentiation was performed according to the instruction manual of the human ES cell Primer Array (Takara Clontech).
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bioRxiv - Microbiology 2024Quote: ... Yeast cells containing pGBKT7PA28γ were grown in yeast extract-peptone-dextrose medium and transfected with a human foetal brain plasmid library based on pACT2 (Clontech Takara). Clones (22.4 × 106 ...
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bioRxiv - Cell Biology 2020Quote: The yeast strain AH109 was co-transfected with bait vector full-length human SNX4 or Lamin cloned into pGBKT7 (Clontech, Oxford, UK) against a pray library of FL SNX-BARs ...
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bioRxiv - Biochemistry 2022Quote: ... DNA ligation of the linearised pOPINTTGneo vector and the human UGGT1insert was achieved by In-fusion™ligation-independent cloning (Takara Ltd.)
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bioRxiv - Cancer Biology 2022Quote: ... Plasmid pMSCV-GADD34-puro contains the human PPP1R15A (GADD34, NM_014330.5) gene subcloned into the NcoI/EcoRI sites of the retroviral vector pMSCV-puro (Clontech Laboratories, Mountain View, CA).
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from RAW264.7 cells and ground cartilage from human tibial plateaus using TRIzol reagent (Takara Bio Inc., Shiga, Japan). For mRNA quantification ...
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bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
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bioRxiv - Developmental Biology 2023Quote: ... Primer specificity and amplification efficiency for genes of interest were verified by performing reactions on a dilution series of human reference cDNA (Takara, cat# 636693). Gene expression relative to GAPDH and RPLP0 house-keeping genes was calculated in Microsoft Excel ...
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bioRxiv - Molecular Biology 2024Quote: Lenti-X™ 293T: This cell line is a sub-clone of human embryonic kidney (HEK) cells for lentivirus particle production (Takara Bioscience). It delivers high transfection efficiency and supports the expression of viral proteins.
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bioRxiv - Cell Biology 2024Quote: ... RAB7A was cloned from the cDNA synthesized from total human brain RNA (Khan et al., 2015) into the pmCherry-N1 plasmid (Clontech Laboratories, 632523). Cells were stained with DAPI ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was synthesized using a reverse transcription system kit (PrimeScript RT Reagent Kit, TaKaRa, RR037B) and oligo(dT ...
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bioRxiv - Neuroscience 2024Quote: ... By means of an RNA library kit (Takara SMART-Seq v4 Ultra Low Input RNA kit) mRNA-seq libraries were prepared using 1ng of RNA purified from the isolated Tbr2-labelled nuclei ...
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bioRxiv - Systems Biology 2020Quote: ... SMARTer Stranded RNA-Seq Kit (Clontech) was used according to the manufacturer’s instructions to prepare sequencing libraries ...
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bioRxiv - Cell Biology 2020Quote: The In-Fusion Cloning Kit (Takara) and the In-Fusion Cloning primer design tool were used for primer design ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using the In-Fusion Kit (Clontech). The mir-137 and miR-122 sequence was thus under the control of the constitutive human RNA Polymerase III promoter U6.
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bioRxiv - Cell Biology 2021Quote: ... and PCR Clean-Up Kit (Takara) then In-Fusion (Takara ...
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bioRxiv - Plant Biology 2020Quote: ... In-Fusion HD Cloning Kit (Takara) was utilized to construct plasmid vectors ...
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bioRxiv - Microbiology 2020Quote: The SMARTer Pico RNA Kit (Clontech) was used for library preparation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PrimeScript RT Reagent Kit (Takara, RR037A) was used to generate cDNA ...
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bioRxiv - Genomics 2020Quote: ... MiniBEST Plant RNA Extraction Kit (TaKaRa) was used to isolate total RNA ...
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bioRxiv - Cell Biology 2020Quote: ... or PrimeSTAR DNA Polymerase kit (Takara). PCR products were run on agarose gel to visualize the fragment size ...
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bioRxiv - Cell Biology 2021Quote: ... Using mycoplasma detection kit (Takara, 6601), we confirmed no contamination in Neuro2A cell line ...
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bioRxiv - Neuroscience 2020Quote: ... a Calcium Phosphate Transfection kit (Clontech) was used to transfect cells with a mixture of OXTR-iTango2 DNA plasmid vectors with a secreted alkaline phosphatase (SEAP ...
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bioRxiv - Microbiology 2021Quote: ... Then the Reverse Transcription Kit (Takara) was used to reversely transcribe RNA to cDNA ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). pIZ-Siwi-D670A and pIZ-BmAgo3-D697A mutants were generated by site-directed mutagenesis.
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). For Gtsf1L ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-PCR kit (Takara, Tokyo, Japan) was used to construct reverse transcription to collect cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... using the Infusion cloning kit (Takara). Ligations were transformed in DH5α cells plated on LB+ Kanamycin plates ...
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bioRxiv - Microbiology 2023Quote: ... PrimeScript™ RT Reagent Kit (TAKARA) was used to generate cDNA and FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... In-Fusion HD Cloning Kit (Clontech), and primers which included NNK codons ...
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bioRxiv - Genomics 2023Quote: ... SMART-Seq v4 kit (Clontech, 634893) was used for the reverse transcription and cDNA amplification according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion Cloning kit (Takara).
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bioRxiv - Immunology 2023Quote: Using PrimeScript RT reagent Kit (TaKaRa), 0.5 µg of total RNAs were reverse transcribed into complementary DNA ...
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bioRxiv - Cell Biology 2022Quote: ... with the SYBR premeraseTaq kit (TAKARA). Amplified mRNA levels were normalized to 18s mRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... SMARTer PCR cDNA synthesis kit (Clontech) was used according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... using Infusion kit (Takara catalog # 638949). For some enhancers we instead cloned into a next generation reporter vector backbone that includes a SYFP2-P2A-3xFLAG-H2B reporter for detection of cytosolic SYFP2 and nuclear FLAG for simultaneous expression analysis and snRNA-seq ...
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bioRxiv - Molecular Biology 2023Quote: The Universal GenomeWalker 2.0 kit (Clontech Laboratories Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... In-Fusion HD Cloning Kit (Clontech) was used for this reaction.
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of produced viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... and PrimeScriptTM RT reagent kit (Takara). RT-qPCR was performed using QuantiNova Probe PCR Kit (Qiagen ...
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bioRxiv - Genetics 2024Quote: Transcription Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of produced viruses was routinely checked by silver staining (Pierce ...