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Citations for Takara Bio :
5351 - 5400 of 6263 citations for Triiodothyronine T3 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Total RNA (500 ng) from the worms was reverse transcribed into cDNA by using a PrimeScript RT Reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared from 2 µg of each RNA sample using the PrimeScript II 1st strand cDNA synthesis kit (TaKaRa, Shiga, Japan) with 40 units of RNasin Plus RNase inhibitor (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and a separate master mix was prepared for each primer set using the TB Green® Premix Ex Taq II (TLi RNaseH Plus) reagent kit (RR820A, Takara Bio). The final master mix contained primers at a concentration of 0.4 µM and a 1X concentration of TB Green® Premix Ex Taq II (TLi RNaseH Plus) ...
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bioRxiv - Bioengineering 2022Quote: 500 ng of total RNA extracted from cell culture were subjected to reverse transcription, using ISOGEN II (NIPPON GENE, Tokyo, Japan) and PrimeScript RT reagent Kit (TaKaRa, Kusatsu, Japan). The resulting cDNA was subjected to real-time PCR analysis using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2020Quote: For SVZ tissue libraries were prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio USA) performing 10 cycles of amplification ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... served as template in a 20 μl qRT-PCR using the TB Green Premix Ex Taq II (Tli RNaseH Plus) kit (Takara Bio Inc.) and the Roche LightCycler 96 system (Roche ...
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bioRxiv - Developmental Biology 2021Quote: SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Plant Biology 2021Quote: ... A 1 μg aliquot of RNA was used for the synthesis of the cDNA first strand using a PrimeScriptTMRT reagent Kit containing gDNA eraser (TaKaRa, Shiga, Japan). The cDNA was used as the template and primers in TableS1 were used to PCR amplify the sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... Pooled RNA from two previously identified positive NP swab samples was used for first strand cDNA synthesis (Takara primescript kit cat # 6110A). 20 μl of the cDNA reaction was diluted ten-fold and used for primary PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The chip was then loaded again on Fluidigm C1 and cDNA was synthesised and pre- amplified in the chip using Clontech SMARTer kit (Takara Clontech, Cat# 634833). In batch B ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The RNA was eluted according to the protocol in 10 uL nuclease free water and equal amounts of RNAs from different samples were immediately subjected to amplification-based cDNA synthesis using the SMARTer PCR cDNA Synthesis Kit (Takara, 634925, 639207). We have standardized the amplification cycle to 15 for 2-10 ng of initial RNA template used for the reaction ...
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bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvest 24 hours post-infection and RNA was extracted by a viral RNA extraction kit (Takara, Cat no. 9766). The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... After the immortalization procedure the absence of virus was verified using the Lenti-X p24 Rapid Titer Kit (Takara Bio USA, #632200).
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bioRxiv - Microbiology 2021Quote: Equal amounts of RNA were converted into cDNA using Prime Script™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (RR047A, Takara-Bio) and then diluted with 80μl nuclease-free water ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were later processed for single cell RNA-seq using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Cat# 634894) as previously described41,45.
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bioRxiv - Neuroscience 2021Quote: ... were produced and purified in the house using an established protocol (Challis et al., 2019) and commercial purification and titration kits (Takara Bio, USA).
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bioRxiv - Plant Biology 2020Quote: ... and one microgram of the DNase-treated RNA was used for cDNA synthesis using PrimeScript RT reagent kit (Takara Bio, CA, USA). The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: 1 μg of RNA was reverse transcribed into cDNA using Prime Script™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (RR047A, Takara-Bio) and then diluted 5-fold with nuclease-free water ...
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bioRxiv - Pathology 2022Quote: ... and expected amplicons of NS1 and VP1 genes were purified using Nucleospin® Gel and PCR clean-up kit (Takara Bio, Japan). Purified PCR amplicons were individually subjected to Barcode-Tagged sequencing (Celemics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Genetics 2022Quote: SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized to a total of 500ng RNA in a 20 μL system using PrimeScript™ RT Reagent kit (TAKARA, #RR036A). q-PCR were performed on anlytik jena (qTOWER 2.0 ...
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bioRxiv - Immunology 2022Quote: ... SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Neuroscience 2022Quote: ... double-stranded cDNA whole transcriptome library was synthesized from 1µg of total RNA with Takara Bio’s SMARTer Stranded Total RNA Sample Prep Kit – HI Mammalian (Takara Bio, Cat. No. 634876) and SMARTer RNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was perfor med using a qPCRsoft (Analytik Jena AG, Germany) with a SYBR®Green PC R Master Mix Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2019Quote: ... the cDNA ends of both genes were obtained via 5’- and 3’-RACE using the SMARTer™ RACE cDNA amplification kit (Clontech, USA). Resulting PCR products were directly sequenced and full-length cDNAs of the putative mudskipper desaturase and elongase were constructed by aligning overlapped regions of the cDNA fragments.
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bioRxiv - Evolutionary Biology 2019Quote: ... This vector was generated by splicing PCR products of the MpALSm gene with the 35S promoter together with the MpEF1α promoter into a XhoI/PstI-digeststed pCAMBIA backbone using the Clontech In-Fusion kit (Clontech Cat. #: 638909) and the primers indicated in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: The transcription initiation and termination sites of OGRU were detected by 5’ and 3’ RACE using a SMARTer® RACE 5’/3’ Kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Full-length cDNA fragments were transcribed and amplified from poly-A RNA in each single cell using the SMARTer Ultra Low RNA kit (Takara Bio, 634832). Tagmentation of cDNA was performed and sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... The libraries for RNA sequencing were generated using the SMART-Seq v.4 Ultra Low Input RNA kit (Takara Bio, cat. #634890) and Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription (RT) was performed with 200 ng total RNA using the Prime Script RT reagent Kit with gDNA Eraser (Takara, Dalian, P.R.China) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... RNA was isolated with Phenol-Chlorophorm and library preparation was carried out with the SMARTer®Stranded RNAseq kit (Takara Bio 634839), according to manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 3′ and 5′ rapid-amplification of cDNA ends Polymerase Chain Reaction (RACE-PCR) was carried out by using the SMART™ RACE cDNA Amplification Kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Low cell RNA-seq libraries (unstranded) wer generated withSMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara/ Clonetech; Cat#634890). All sequencing libraries were sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Genomics 2019Quote: ... The Agilent 2100 Bioanalyzer was used to assess RNA quality and only high-quality RNA (RIN > 8) was further processed for cDNA synthesis with SMART-Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized and amplified using SMART-seq® V4 Ultra® Low Input RNA kit for Sequencing (Takara Bio USA, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Libraries from RPFs isolated from HEK293T cells were prepared using the SMARTer® smRNA-Seq Kit for Illumina (Takara, Cat. No. 635029) and sequenced with 50 cycles single-read on an Illumina NovaSeq 6000 sequencer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Labelled amplicons were pooled and purified using NucleoSpin Gel and PCR Clean-up kits (Takara Bio USA, Inc, Mountain View, CA USA). Libraries were sequenced in-house using a NextSeq 500 High-Throughput Sequencing System (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... 5-AAC TTC GCG CTT CCT AAG TCC CCG AAG GA-3 using Prime STAR mutagenesis kit (Takara Bio Inc. Shiga, Japan). GFP-Rab27a was purchased from RikenBRC(Tsukuba ...
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bioRxiv - Microbiology 2021Quote: ... RNA-Seq library preparation was performed using the SMARTer® Stranded Total RNA-Seq kit – Pico Input Mammalian (Takara Bio USA, Inc) with the RNA input ranging from 7 to 10 ng of starting material.
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bioRxiv - Evolutionary Biology 2020Quote: ... we changed the CaMAL2 promoter to the ScGAL1 promoter using an In-Fusion kit (Takara Bio USA Inc., Mountain View, CA, USA) to generate pSFS4A plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR analysis was performed using the TB Green® Premix Ex Taq™ Tli RNaseH Plus kit (TAKARA, Mountain View, CA). The gene-specific primer sequences were as follows ...
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bioRxiv - Microbiology 2019Quote: ... A pair of primers that match the sr8384 sequence were separately added into the total RNA to synthesize the first-strand cDNA by using the PrimeScript RT Reagent Kit (TaKaRa, cat. #RR047A). Next ...
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bioRxiv - Microbiology 2019Quote: Rapid amplification of cDNA ends (RACE) was performed using reagents from an In-Fusion® SMARTer® Directional cDNA Library Construction Kit (TaKaRa) and custom primers ...
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bioRxiv - Physiology 2019Quote: cDNA library was prepared using SMART-Seq v4 3’ DE kit according to the manufacturer’s instructions (Clontech Laboratories Inc. Cat. No. 635040). For each clone ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time qPCR was performed in 48-well blocks with a StepOne Plus Real Time PCR System by using TaKaRa SYBR Premix Ex Taq II reagent kit (TaKaRa, Tokyo, Japan). All qRT-PCRs were performed in triplicate using total RNA samples extracted from three independent biological replicates ...
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bioRxiv - Genomics 2021Quote: ... Purified RNA samples were diluted to 1 μg/μL and pooled to perform cDNA synthesis by utilizing PrimerScript first strand cDNA synthesis kit (Cat. no. 6110A, Takara Bio, Japan), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... To determine the 5’ and 3’ UTR of the sds3 gene 5’ and 3’ Rapid amplification of cDNA ends (RACE) was performed using the SMARTer® RACE 5’/3’ Kit (Takara Bio) on RNA isolated using Trizol (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... First-round reverse transcription PCR (RT-PCR) was conducted by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). A 10 μL reaction mixture contained 5 μL of 2 × 1 Step Buffer ...