Labshake search
Citations for Takara Bio :
5301 - 5350 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... RNA was eluted in 10 µl water and immediately proceeded to cDNA generation using the SMART-Seq V4 Ultra Low RNA Kit (Takara Bio USA, Mountain View, CA). 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... recombinant sites including the 900 base pair upstream/downstream flanking region and the 100 base pair coding sequence of the target genes were cloned into pK18-CmR-pheS** plasmid backbone by using an In-Fusion® HD Cloning Kit (TAKARA Bio; Kusatsu, Shiga, Japan) to generate the plasmid (aedF- ...
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bioRxiv - Zoology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed using PrimeScript® RT reagent kit with gDNA Eraser (RR047A, Takara Bio Inc., Dalian, China). The gene expression was determined through RT-PCR ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: Antibody sequencing was performed as previously described (Simonich et al., 2019; Vigdorovich et al., 2016) using the SMARTer RACE 5’/3’ Kit (Takara Bio USA Inc., Mountainview, CA). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Microbiology 2019Quote: ... 300 ng of each RNA was reverse transcribed to cDNA using random hexamers and oligo(dT) primers and following the instructions provided in the PrimeScript RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Flash frozen cell samples were sent to the Cambridge Genomic Services for library preparation using SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA, Inc), followed by RNA sequencing (NextSeq 74 cycle high output 15-20 M reads per sample ...
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bioRxiv - Physiology 2020Quote: ... The PCR products were subsequently cloned into an EcoRI-HF® (New England BioLab, MA, USA) linearized pIRESZ2_ZsGreen1 vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP). After sequence validation (Eurofin ...
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bioRxiv - Genetics 2020Quote: ... nucleotides 610–792 of the map3 ORF were subjected to random mutagenesis by using the Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, Mountain View, CA, USA) in accordance with the manufacturer’s instructions for error-prone PCR ...
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bioRxiv - Plant Biology 2021Quote: ... and the library preparation was made with the SMART-Seq™ v4 Ultra™ Low Input RNA Kit for Sequencing (Takara, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs that were used for RACE were synthesised from 1 µg of polyA+ RNA using a SMART RACE cDNA Amplification Kit (Clontech Laboratories, Inc., Palo Alto, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 ng RNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Europe SAS). Libraries were prepared from the purified cDNA using the Nextera™ XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... The amplified 0.5 kbp fragment was inserted into the BamHI site of pTK2051 using the In-Fusion Snap Assembly kit (Takara Bio USA, Mountain View, CA, USA). The resulting suicide plasmid was introduced into B ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR products were subsequently cloned into pGEMHE Xenopus laevis oocyte expression vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP) and the final sequence validated (Eurofin ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were amplified and synthesized into cDNA according to the manufacturer’s protocol using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Inc., Shiga, Japan) and amplification yield was checked using a Bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragments were then fused with SmaI-linearized pUt-C-EGFP10 using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). Gene sequences fused with the egfp gene were positioned in tandem between the linker sequences ...
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bioRxiv - Microbiology 2022Quote: ... DNAse treated-RNA was reverse transcribed into cDNA using random hexamers and oligo (dT) primers with the Prime-Script RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). Viral presence was assessed through RT-qPCR (StepOnePlus Real-Time ...
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bioRxiv - Cancer Biology 2024Quote: We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC-97L cells using the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The full-length CD177 cDNA inserts were sub-cloned into pBMN-I-EGFP retroviral vector digested with Bam HI and EcoR I restriction enzymes using InFusion HD Cloning Kit (Takara Bio USA, Mountain View, Ca) according to the product manual ...
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bioRxiv - Bioengineering 2023Quote: ... Purification and titer determination of the adenoviral particles were carried out using the Adeno-X™ Maxi Purification Kit (Takara Bio USA, San Jose, CA) and QuickTiter™ Adenovirus Titer ELISA Kit (Cell Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The amplified fragments were then fused with SmaI-linearized pisCIIA-mCherry (Mamun et al, 2020) using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). Recombinant plasmids for the expression of mCherry-fused 19 proteins were digested with NotI and ectopically inserted into the ATP sulfurylase (sC ...
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bioRxiv - Immunology 2023Quote: ... the library was prepared by synthesizing cDNA via reverse transcription and amplifying cDNA by long-distance PCR using the Takara SMARTer v4 kit (Takara Bio USA, San Jose, CA). 2×150bp sequencing was performed on an Illumina NovaSeq6000 instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... IVF embryos were lysed in lysis buffer as described and cDNA was prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio, catalong #634890). cDNA was tagmented using Nextera XT DNA Library Preparation Kit (Illumina catalog #FC-131-1024 ...
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bioRxiv - Molecular Biology 2023Quote: ... were constructed by cloning the full-length coding region into the pGADT7 vector using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). The Matchmaker two-hybrid system 3 (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were sized in gels (as above) and bands of interest isolated using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio Inc., San Jose, CA). Sanger Direct Sequencing was performed at Azenta Life Sciences (South Plainfield ...
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bioRxiv - Plant Biology 2024Quote: ... and a 500-ng RNA was used for the first-strand cDNA synthesis using PrimeScript™ 1st strand cDNA Synthesis Kit (6110A; TaKaRa, San Jose, California, USA) with oligo(dT ...
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bioRxiv - Physiology 2024Quote: Total RNA (1 µg) was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. # 634875; Takara, Saint-Germain-en-Laye, France). Sequencing was performed on the NextSeq 2000 instrument (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng of RNA was used as input for cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio, San Jose, CA, USA). Library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Physiology 2024Quote: Libraries were prepared by the Van Andel Genomics Core (Grand Rapids, MI) from 1 ng of total RNA using Takara SMART-Seq Stranded Kit (Takara Bio USA, Mountain View, CA) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using the hetL pET28 fw and hetL pET28 rv infusion primers and cloned into the XhoI and NcoI restriction sites of the pET28a expression plasmid using the In-Fusion technology (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Microbiology 2020Quote: ... was amplified using the patS pRL fw and patS pRL rv infusion primers and cloned into the BamHI restriction site of the pRL1272-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Microbiology 2020Quote: ... was amplified using the hetL pRL fw and hetL pRL rv infusion primers and cloned into the BamHI restriction site of the pRL25T-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Developmental Biology 2020Quote: ... were transferred into 0.2-mL PCR tubes and processed for sequencing using Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA, Mountain View, CA) as described in Wood et al ...
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bioRxiv - Molecular Biology 2021Quote: ... the puromycin-selected transfectants were directly subjected to RT-qPCR analysis using the CellAmp™ Direct TB Green® RT-qPCR Kit (TaKaRa Bio Inc., Shiga, Japan) at 3 days after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... and 19 and BIRC2 was amplified using specific primers (Supplementary File 1) and cloned downstream of luciferase gene into pcDNA6/Luc(30) using an In-Fusion HD cloning kit (Takara Bio USA, Inc., Mountain View, CA). The recombinant pcDNA6/LUC-DUSP (or pcDNA6/LUC-DUSP-211Δ) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we performed Rapid-Amplification-of-cDNA-Ends (RACE) PCR using transcript-specific primers and the SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc., Mountain View, CA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Plant Biology 2021Quote: ... and 500 ng total RNA was incorporated for first-strand cDNA synthesis using the M-MLV reverse transcriptase kit (TaKaRa Bio USA, Mountain View, CA, USA), and the library was used as a template for qRT-PCR analysis ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 600 pg of RNA was used as input for the synthesis of cDNA with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA, Mountain View, CA, USA). Manufacturer suggested protocol was followed ...
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bioRxiv - Biochemistry 2019Quote: ... cochleariae fat body for 3’ rapid amplification of cDNA ends (3’ RACE) was synthesized according to the manual from SMARTer RACE 5’/3’ Kit (Takara Bio, Inc. Mountain View, CA, USA). All cDNA templates were stored at −20 °C after synthesis.
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bioRxiv - Microbiology 2020Quote: The open reading frame of hetL gene mutated to encode for a D151A substitution was amplified using the hetL pRL fw and hetL pRL rv infusion primers with pKT25-hetLD151A as template and cloned into the BamHI restriction site of the pRL25T-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit with nonstranded and polyA+ selection (Clontech, Takara Bio USA, Mountain View, CA, USA). Approximately 120 million 125-bp paired-end reads per sample were obtained (HiSeq 2500 ...
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bioRxiv - Immunology 2022Quote: ... an amount of 1 ng of total RNA was used as input for the SMART-Seq® Stranded Kit (Cat. No. 634444; protocol version “022829”; low input option, Takara Bio USA, San Jose, USA). This kit can deal with degraded as well as high-integrity input RNA ...
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bioRxiv - Immunology 2023Quote: ... Library construction was performed based on manufacturer’s recommendation for the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA Inc., California, USA) followed by the Nextera® XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: We prepared each sequencing library from 1 μg of total RNA using a PrepX PolyA mRNA Isolation Kit (Wafergen, 40047) on the Apollo 324 NGS Library Prep System (Takara Bio USA Holdings, Mountain View, CA). We added Illumina indices and amplified libraries with 12 cycles of PCR and cleaned them using PCRClean DX beads (Aline Biosciences ...
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bioRxiv - Biophysics 2023Quote: Plasmids used in this study (Supplementary Table S1) were assembled by Polymerase Chain Reactions (PCR) amplifying insert and vector DNA fragments followed by In-Fusion cloning (Takara Biosciences, In-Fusion HD Cloning Kit). Oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Samples showing successful RNA extraction after checking by Bioanalyzer electrophoresis were reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio. Inc., Shiga Prefecture, Japan). Successfully amplified samples were prepared for paired end short-read sequencing using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR1 (10 cycles of amplification) was performed with the addition of SMARTer RNA Unique Dual Index Set A and Set B (Takara SMARTer RNA Unique Dual Index Kit-96A). Samples were pooled into quadrants and depletion of ribosomal cDNA was performed ...
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bioRxiv - Zoology 2019Quote: Amplification of 5ʹ and 3ʹ ends of cDNA was performed according to the protocol described in the SMART RACE cDNA Amplification Kit (Takara Bio USA, Inc. Mountain View, CA, USA). Three pairs of gene-specific primers (GSP) ...