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Citations for Takara Bio :
5301 - 5350 of 5473 citations for Arg8 Vasopressin AVP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... RNA was reverse transcribed and cDNA amplified by in vitro transcription with the TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa, Cat. No. 634889) or a low input method using NEB Next® Ultra RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The synthesized sequences were ligated to an expression construct encoding a truncated form of OsPHR (Δ1-51OsPHR) using restriction enzyme cloning or InFusion cloning (InFusion HD Cloning kit, Takara Bio, Shiga, Japan). The modified PHR was cloned into the binary vector pMpGWB106 (Ishizaki et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... high-quality cDNA was generated from the total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio, Cat. #634889), and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Molecular Biology 2022Quote: ... The double stranded barcode library was subsequently cloned into the linearized vector using the In-Fusion® HD Cloning Kit (Clontech® Laboratories) following instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: RNA was reversely transcribed into 1st strand cDNA in a 20 μL reaction volume using the PrimeSeriptTM1stStrand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer’s protocol of Reverse Transcription System ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed by targeting the SARS-CoV-2 RdRp gene as follows: each 20 µl sample consisted of 12.5 µl One Step PrimeScript™ III RT-PCR Kit (Takara Bio, Shiga, Japan), 0.5 µM Sars-CoV-2 CRV forward primer (5’-TCACCTAATTTAGCATGGCCTCT-3’) ...
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bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolation of the upstream genome sequences of gnrh1 and gnrh3 of piranha was performed using the Universal GenomeWalker ™ kit (Takara Bio USA) according to the instructions ...
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bioRxiv - Bioengineering 2023Quote: ... CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa, San Jose, CA, USA). Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd ...
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bioRxiv - Neuroscience 2023Quote: ... then cDNA was synthesized using the SMART-Seq® v4 Ultra® Low Input RNA kit (Takara Bio USA Inc. Cat No. 634888). Libraries were prepared using the NexteraTM DNA Flex Library Prep (Illumina Cat No ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The first-strand cDNAs of mRNA and mature miRNA were synthesized from 500 ng of total RNA using PrimeScript™ RT reagent kit with gDNA Eraser (Cat# RR047A, Takara, Kyoto, Japan) and miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Cat# KR211 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1μg of isolated RNA was taken from each sample was then transcribed to cDNA using PrimeScript ™ RT reagent kit with gDNA Eraser (Takara Bio RR047B). According to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Each of the four pools was converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit (Takara Bio Catalog# R400740), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA amount equivalent to 12 ng was used to prepare the sequencing library using SMARTer® smRNA-Seq Kit for Illumina® (Takara 635031) according to the kit manual till cDNA synthesis ...
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bioRxiv - Biochemistry 2023Quote: ... The synthetic genes were cloned into the NdeI/XhoI site of the pET-28a(+) expression vector by In-Fusion cloning using the In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan), yielding expression constructs with an N-terminal hexahistidine tag and thrombin tag ...
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bioRxiv - Cell Biology 2023Quote: ... Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411) and sequenced on a Novaseq 6000 ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extraction and genomic DNA removal were performed using a Total RNA Extraction Kit (Plant) (RBC Bioscience, New Taipei, Taiwan) and DNase I (Takara Bio, Shiga, Japan) following the manufacturers’ protocol ...
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bioRxiv - Bioengineering 2023Quote: ... T-2A-H2B-tdTomato was generated by replacing the EGFP fragment with H2B-tdTomato using the In-Fusion® HD Cloning Kit (Catalog #639648, Takara, Kusatsu, Japan), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used for complementary DNA (cDNA) synthesis by PrimeScript first strand cDNA synthesis kit (TAKARA-Bio, Cat# 6110) using an oligo(dT ...
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bioRxiv - Genomics 2024Quote: The Iso-Seq libraries were constructed from 1 µg of total RNA per sample and full-length cDNA were then generated using the SMARTer PCR cDNA synthesis kit (Takara Bio Inc, 639506). The libraries were sequenced on the Sequel Instrument v1 ...
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bioRxiv - Microbiology 2023Quote: ... The DNA free RNA was used for cDNA preparation using Takara first strand cDNA synthesis kit (cat no.6110A, Takara Bio Inc., Japan) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from the above total extracted RNA as a template using a PrimeScript First-Strand cDNA Synthesis Kit (Takara Bio, Beijing, China). Specific primers (Table 1 ...
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bioRxiv - Immunology 2023Quote: ... which was measured using a real-time PCR assay with a SARS-CoV-2 direct detection RT‒qPCR kit (Takara Bio, Siga, Japan). The IC50 was calculated by IC50 Calculator (https://www.aatbio.com/tools/ic50-calculator ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained by reverse-transcribing the total RNA extracted from HCT116 cells using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Dalian, China) was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio) ...
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bioRxiv - Bioengineering 2023Quote: ... 3µg of DNA was digested by EcoRI and the digested DNA was self-ligated using DNA Ligation Kit (Mighty Mix; Takara Bio, Kusatsu, Japan) after purification by ethanol precipitation ...
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bioRxiv - Plant Biology 2023Quote: ... Each one or two PCR fragments were inserted into pMpGWB300 that was cleaved by HindIII and SalI using the In-Fusion Cloning kit (TaKaRa Bio, Kusatsu, Japan) to generate pMpGWB300:proMpRKD:MpRKD and pMpGWB300:proMpRKD:mMpRKD plasmids.
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bioRxiv - Plant Biology 2023Quote: ... cDNA from a microgram of RNA was obtained using a PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan) following its instructions ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was removed from 1 μg purified RNA using gDNA Eraser (PrimeScript RT Reagent Kit, Perfect Real Time) following manufacturer’s instructions (Takara Bio Inc., Kusatsu, Japan). Reverse transcription and cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa Bio) ...
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bioRxiv - Genetics 2024Quote: 1 µg of gDNA Eraser-treated RNA was reverse transcribed into cDNA using the PrimeScrip® RT reagent Kit with gDNA Eraser (Takara Bio, Japan), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 20 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio USA. Inc), which converts poly(A ...
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bioRxiv - Neuroscience 2024Quote: Library preparation and RNA sequencing were performed by Novogene Co using the SMART-Seq v4 PLUS Kit (Takara Bio, Kusatsu, Shiga, Japan, # R400752). Total RNA was reverse transcribed into the first-strand cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification of the fragments and their subsequent ligation was performed using the In-Fusion cloning kit and online tools (BD Clontech, Takara Bio, USA), or they were synthesized by GenScript (Piscataway ...
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bioRxiv - Cell Biology 2024Quote: A total of 250 pg to 10 ng RNA per sample was used as input material for sequencing libraries (1–500 ng for small RNA libraries) using the SMARTer Stranded Total RNA-Seq Kit V2 (Takara Bio USA, Inc.) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Reverse-transcription and amplification were performed using the One Step PrimeScript™ RT-PCR Kit (RR064A, Takara Bio Inc., Kusatsu, JP-25, Japan) with the following forward primer (5’-ATGAGYCTTYTAACCGAGGTCGAAACG-3’ ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was reverse transcribed from 1 μg of total RNA using the PrimeScriptTM RT reagent kit (AK4201; Takara Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... A first-strand cDNA was obtained from 1 μg of total RNA using a PrimeScript® RT reagent kit (Takara Bio, Inc., Otsu, Japan). The sequences of candidate genes were obtained from the local alkaligrass EST database using a BLASTn program ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng of RNA was used to prepare cDNA sequencing libraries with the Clontech SMARTer Stranded Total RNA-Seq kit (Pico Input) with ribosomal cDNA depletion (Takara Bio USA, Cat #634413). Prepared libraries were submitted to paired-end 100 bp sequencing on a NovaSeq 6000 system at the YCGA ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA carrying full-length transcript information was synthesized with SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TaKaRa Bio, Japan) followed by Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology, Dalian, China). For sequencing analysis of the PxRdls ...
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bioRxiv - Developmental Biology 2020Quote: ... Lentiviral supernatant titers were determined by Lenti-X p24 Rapid Titer Kit (supplemental Table 1) according to manufacturer’s protocol (Takara Bio USA, Inc. California, U.S.A).
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bioRxiv - Plant Biology 2019Quote: ... strategy and internal primers designed from the genomic contigs (Table S1) following the kit supplier’s recommendations (Takara Bio Europe/Clontech, Saint Germain-en-Laye, France). The 3’ ends were amplified using forward internal and polyA-anchored LD primers (Table S1 ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCR product was cloned into pcDNA6/luc/NP3 into a NotI-AgeI site using the Clontech In-Fusion kit (Clontech Laboratories, Mountain View, CA). To remove the two putative MIR211 sites ...
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bioRxiv - Genomics 2019Quote: ... PCR products were collected by centrifugation at ~2250 xg for 20 min using the supplied SMARTer ICELL8 Collection Kit (Takara Bio USA, Cat.#640048).
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bioRxiv - Microbiology 2019Quote: ... and then cloned into the pC003 vector by replacing the shahiiCas13a locus using the In-Fusion HD Cloning Kit (Takara Bio, Inc., Shiga, Japan), and then kanamycin resistant gene of the vector was replaced by kanamycin-resistant gene (aphA-3 ...