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Citations for Takara Bio :
5201 - 5250 of 5458 citations for Rat Insulin Like Growth Factor Binding Protein 4 IGFBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized and amplified using template switching technology of the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Cat. #R400752), followed by purification using the Agencourt AMPure XP Kit (Beckman Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... Then the first-strand cDNA synthesis was primed with an oligo (dT) primer by using a PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... a 4.0 kb fragment containing the ERF019 gene and its native promoter was amplified and inserted into pBI101 with BamHI and SacI by In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan). For awf2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Amplified cDNA from the ICELL8® 3’ DE Chip individual cells was collected and pooled using the ICELL8® Collection Kit (Takara Bio) by centrifugation at 3,200 x g ...
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bioRxiv - Cancer Biology 2020Quote: ... and used to produce low input Illumina Fragment libraries using a Low Input Library Prep kit v2 (Clontech Laboratories, Inc., Catalog No. 634899). DNA was fragmented using the Covaris S220 sonic disruptor and libraries were then sequenced as paired end reads with a read length of 100bp on an Illumina HiSeq 3000.
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg total RNA (20 μL reaction volume) using a PrimeScript™ 1st Strand cDNA Synthesis Kit (Takara, Dalian, China) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... pChlamiRNA3int (obtained from the Chlamydomonas Resource Center) in between Xho1 and Not1 restriction sites by In-fusion HD EcoDry cloning plus kit (Takara, Cat. No. 638915). A gene fragment encoding three copies of the 9-amino-acid HA epitope followed by EcoR1 and XbaI restriction sites was inserted using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent technologies) ...
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bioRxiv - Systems Biology 2021Quote: ... 6 genes were generated as inserts in the shuttle vector puC57 and were subsequently cloned into pDM323 between BglII and SpeI sites using the In-Fusion® HD Cloning Kit (Takara Bio USA) and confirmed by sequencing ...
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bioRxiv - Microbiology 2021Quote: 5’-RACE PCR was performed on total RNA from VSVg-NL43 infected CD4+ T cells and MDMs following the manufacturer’s protocol for SMARTer® RACE 5’/3’ Kit (Takara Bio, Cat: 634858). Random primers were annealed to template RNA using 10X Random Primer Mix ...
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bioRxiv - Microbiology 2021Quote: ... The LAMP1 gene was then sub cloned into the pEF1α-mCherry-N1 vector using the In-Fusion® HD Cloning Kit (Clontech, Takara, Japan), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: pEX18Tc-hpdA was constructed for gene knockout by fusing the erythromycin resistance gene and two upstream and downstream fragments of the target gene amplified with the primers shown in Table 3 to Sac I/Hind III-digested pEX18Tc with the In-Fusion® HD Cloning Kit (TaKaRa, Dalian, China). The resulting plasmid pEX18Tc-hpdA was transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... The amplification of viral DNA by PCR was carried out base on the manufacturer’s recommendations of the LA PCR Kit (TaKaRa Biomedical Technology Beijing, China). Briefly ...
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bioRxiv - Microbiology 2020Quote: The lentivirus-based expression plasmids were generated with a pLOV-CMV-GFP vector (Neuron Biotech, China) using In-Fusion HD Cloning kits (Clontech Laboratories, Inc., USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or its derivatives with appropriate antibiotics resistant genes (ampicillin for Escherichia coli and neomycin or puromycin for mammalian cells) and a tag (SNAP-tag or HaloTag) using an In-Fusion HD Cloning Kit (639635, Takara Bio, Shiga, Japan). The E ...
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bioRxiv - Neuroscience 2022Quote: ... Antennal cDNA was synthesized from 1 μg of antennal total RNA using SMARTer™ RACE cDNA amplification kit according to manufacturer’s instructions (Clontech, Mountain View, CA). To obtain full-length coding sequences ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.25 ng to 10 ng of total RNA was used for cDNA library preparation using a kit suitable for RNA isolation at pico-molar concentrations (MARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian) following manufacturer recommended protocol (Clontech/Takara Bio #635005). Sequencing was performed by the UM DNA Sequencing Core ...
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bioRxiv - Microbiology 2022Quote: The BLV pol gene was measured in the genomic DNA samples of blood and tissue samples of cattle using real-time PCR with a BLV Detection Kit (Takara Bio, Otsu, Japan) with a LightCycler 480 System II (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... the cDNAs were reverse-transcribed from 5 µg of total RNA with the PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Dalian, China). Real time PCR (qPCR ...
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bioRxiv - Developmental Biology 2020Quote: ... The corresponding full-length cDNA library was then generated using 1μg total RNA and the SMARTer PCR cDNA synthesis kit (Clontech, Takara Bio Inc., Shiga, Japan) following PacBio recommendations set out in the Iso-Seq method ...
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bioRxiv - Molecular Biology 2021Quote: ... The chip was then loaded again on Fluidigm C1 and cDNA was synthesised and pre- amplified in the chip using Clontech SMARTer kit (Takara Clontech, Cat# 634833). In batch B ...
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bioRxiv - Neuroscience 2020Quote: ... Single cells were collected into individual wells of 96 well-PCR plates containing 9.5 μL/well of freshly made lysis buffer (provided in SMART-Seq v4 Ultra Low Input kit for Sequencing (Takara Bio USA #634893)) ...
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bioRxiv - Neuroscience 2020Quote: mRNA in the cell lysate was reverse-transcribed and amplified for 25 cycles using the SMART-Seq v4 Ultra Low Input kit for Sequencing (Takara Bio USA #634893) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products of the expected size were purified using the Easy Pure Quick Gel Extraction Kit (Transgen Biotech, Beijing, China) and cloned into TA Vector (Takara Biotechnology, Dalian, China). Positive clones were selected and sequenced by Beijing Genomics Institute (Beijing ...
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bioRxiv - Microbiology 2020Quote: ... One hundred microliter cell culture supernatant was harvested for viral RNA extraction using the MiniBEST Viral RNA/DNA Extraction Kit (Takara, Cat no. 9766) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Transcription start sites were characterised by cloning and sequencing 5’ RACE products using the SMARTer RACE 5’/3’ kit (Takara Bio USA, Inc). In summary ...
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bioRxiv - Bioengineering 2022Quote: ... Reverse transcription to obtain cDNA was performed according to the instructions of the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Biomedical Technology (Beijing) Co. ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was ligated into the NotI site of the pNEN142 vector (Minetoki et al., 2003) with an In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan); this vector contains the improved promoter of the A ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc., Shiga, Japan). Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x ...
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bioRxiv - Cell Biology 2022Quote: ... and Foxa3 were obtained by reverse transcription PCR using a template RNA extracted from a C57BL/6J liver using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TAKARA Bio, Japan). The coding sequences of the primers used are listed in Table S3 ...
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bioRxiv - Developmental Biology 2019Quote: ... First-strand cDNA (from total RNA) was synthesized according to the SMART-Seq™ v4 Ultra™ Low Input RNA Kit protocol (Clontech Laboratories). The PCR-amplified cDNA was purified using SPRI beads (Beckmann Coulter).
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bioRxiv - Molecular Biology 2019Quote: ... A SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Physiology 2020Quote: ... containing 1 μL of 10× reaction buffer (SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, Cat. No. 634888, Takara Bio, Kusatsu, Japan). Nuclease-free water was added to 11.5 μL ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently used as a template to generate all other HaloTag constructs using the In-Fusion HD Cloning Kit (Takara Bio USA, Inc), with the exception of pReciever-M49(HaloTag-SOX17 ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 ng of RNA were used to prepare RNA libraries using the SMARTer Stranded Total RNA-seq Kit – Pico Input Mammalian (Takara Bio USA Inc.). Briefly ...
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bioRxiv - Genetics 2019Quote: ... RNA was reverse transcribed using SMART-Seq Reverse Transcriptase followed by 11 cycles of PCR amplification (SMART-Seq v4 kit, Takara Bioscience, Cat. 634890). Purification and size selection of DNA was carried out using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by RNA isolation according to the manufacturer’s instructions (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing, Takara Bio USA) (Figure 2A ...
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bioRxiv - Molecular Biology 2021Quote: ... One µg of total RNAs was subjected to reverse transcription into cDNAs using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, cat. no. RR047A) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... four sequencing libraries (one for each tissue type) were constructed using the SMARTer PCR cDNA Synthesis Kit (ClonTech, Takara Bio Inc., Shiga, Japan) by tissue type with no size selection by NovoGene ...
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bioRxiv - Plant Biology 2020Quote: ... four sequencing libraries (one for each tissue type) were constructed using the SMARTer PCR cDNA Synthesis Kit (ClonTech, Takara Bio Inc., Shiga, Japan) by tissue type with no size selection by NovoGene ...
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bioRxiv - Microbiology 2021Quote: RsmY and RsmZ expression quantification was done by RT-PCR using Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio USA, Inc). For each reaction ...
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bioRxiv - Plant Biology 2021Quote: Bait constructs for Y1H analysis were produced by inserting promoter regions of SDI genes into the multiple cloning site of pTUY1H using In-Fusion® HD cloning kit (Takara Bio Inc.) and then used to transform yeast strain Y187 ...
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bioRxiv - Developmental Biology 2021Quote: ... and the total RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, RR047A, Kusatsu, Shiga, Japan). The diluted cDNA was used as a template for qPCR ...
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bioRxiv - Microbiology 2019Quote: ... Japan) and cloned into a pEGFP-N1 vector that encodes EGFP [49] using an In-Fusion HD Cloning Kit (TaKaRa, Mountain View, CA). The mouse ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix Ex Taq II (TaKaRa ...
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bioRxiv - Microbiology 2021Quote: ... The extracellular SARS-CoV-2 RNA in the culture supernatant was analyzed using SARS-CoV-2 direct detection RT-qPCR kit (RC300A; TaKaRa-Bio, Shiga, Japan). The infectivity of SARS-CoV-2 in the culture supernatants was determined at 48 h post-infection ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
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bioRxiv - Biochemistry 2021Quote: ... In-fusion cloning was used for the ligation of sequence fragments to vector with the In-fusion HD Cloning Kit (Clontech Laboratories, Inc, US). Site-directed mutations were accomplished according to the protocol provided in a Mut Express II Fast Mutagenesis kit V2 (Vazyme ...
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bioRxiv - Biochemistry 2021Quote: ... vector (including the N-terminal hexahistidine and thrombin tags) by In-Fusion cloning using the In-Fusion HD Cloning Kit (Takara Bio, Kusatsu, Japan), yielding the pBAD-yTrm5 vector ...
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bioRxiv - Biophysics 2020Quote: ... tinctorius Nav1.4 were determined by DNA gel extraction and sequencing after rapid amplification of cDNA ends (RACE) using the SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and internal primers designed from P ...
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bioRxiv - Cell Biology 2021Quote: ... we linearized the pnCS plasmid with NdeI/NheI and employed seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, Cat. # 638910) with the following primers ...