Labshake search
Citations for Takara Bio :
4951 - 5000 of 5458 citations for Rat Insulin Like Growth Factor Binding Protein 4 IGFBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Purified viral RNA (vRNA) was reverse transcribed into cDNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio Inc., Shiga, Japan). The number of RV genome copy in the medium supernatant was determined using RT-qPCR with general or serotype-specific Taqman probe and primers (Additional file 2 ...
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bioRxiv - Genomics 2019Quote: The first-strand cDNA was synthetised from 1 μg of 5 tissues (root, stem, leaf, flower and peel) total RNA using Prime Script RT Reagent Kit (Takara, Dalian, China). The qRT-PCR reactions were performedin 96-well plates using the ABI 7500 fast Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...
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bioRxiv - Molecular Biology 2021Quote: ... were co-transfected into HEK293T cells at a 1:1:1:1.6:4.6 ratio using CalPhos mammalian transfection kit (TaKaRa Clontech, Mountain View, CA #631312) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The same samples used for RNA sequencing were used for reverse transcription and synthesis of cDNA with PrimeScript RT Reagent Kit with gDNA Eraser following the instructions of the manufacturer (TaKaRa, Dalian, China). For designing the primers for qRT-PCR ...
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bioRxiv - Pathology 2020Quote: The viral loads of WHCV in BALF of patient 1 were determined by quantitative real-time RT-PCR with Takara One Step PrimeScript™ RT-PCR Kit (Takara RR064A) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... These four purified products were introduced to the HindIII and BamHI sites of PKNTG using In-Fusion-HD cloning kit (Takara Bio USA). The plasmid was then sequenced and introduced into the ΔFvsec4 strain for genetic complementation and localization study.
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bioRxiv - Developmental Biology 2019Quote: ... The SOX2 PCR product was purified and inserted into CMV-t2a-GFP using the In-Fusion HD cloning kit (Takara Bio USA) and transformed into Stellar Competent Cells (Takara Bio USA) ...
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bioRxiv - Immunology 2019Quote: ... by generating cDNA from 2 ng RNA and 9 PCR cycles per replicate with the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (cat# 634891, Takara) and by enzymatic fragmentation of 300 pg of cDNA followed by 12 PCR cycles using the Nextera XT DNA Library Preparation Kit (cat# FC-131-1096 ...
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bioRxiv - Molecular Biology 2019Quote: ... LINC00662 (NR_027301) and ELK4 (NM_001973.4) were detected by One-Step SYBR PrimeScript RT-PCR Kit (Perfect Real Time; Takara Bio, Inc., Kusatsu, Japan). Relative expression values were calculated using the relative quantification (2−△△Ct ...
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bioRxiv - Plant Biology 2019Quote: ... 1μg purified total RNA was used to obtain the first-strand cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Japan). The Takara SYBR ® Premix Ex Taq™ II was used to perform the qRT-PCR assay using primers P13 and P14 ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified using Nucleo Spin® Gel and PCR Clean-up Kits (Code No. 740609.50; TAKARA Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were gel purified (Machery Nagel) and used to generate α-32P dCTP-labeled probes using the Random Prime Labeling Kit (Takara/Clontech). Probes were purified over nucleotide purification columns (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA (1 µg) was reverse transcribed into First-strand complementary DNA using a PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions and stored at –20°C.
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA and 200 ng of a random primer were used in reverse transcription (RT) through the cDNA synthesis kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... the PC1-CTT (mPC-4119) [6] insert was fused with mCherry and cloned into pcDNA3 using an In-Fusion HD Plus kit (Takara Bio, 638909). The expression plasmids for EGFP-SLK (pEGFP-C1+SKL ...
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Cell Biology 2021Quote: ... or mCherry cDNA were cloned at the position next to S252 of DFNA5 cDNA using an In-Fusion HD cloning kit (TaKaRa, Seoul, Korea). All the lentiviral vectors ...
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bioRxiv - Biochemistry 2020Quote: Complementary DNA (cDNA) was generated from previously extracted total RNA (900 ng) using the SMARTER RACE 5’/3’ kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of purified RNA from each sample was used for reverse transcription reaction to generate cDNA with a high-capacity cDNA reverse transcription kit (Takara, Dalian, China) and the resulting cDNA was then used for qPCR on a real-time PCR detection system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reactions were done on Roche LightCycler® 96 PCR system with the SYBR Premix Ex Taq™ Kit (TAKARA, RR420A). Gene expression levels were normalized to GAPDH and compared to gene expression levels in hESCs ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were purified using Dyne PCR Purification Kit (DyneBio, South Korea) and cloned into pPZP211 vectors using Takara T4 DNA Ligase (Takara Bio, Japan). Escherichia coli (DH5α ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of total RNA was reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Japan). qPCR or qRT-PCR was performed using TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2021Quote: ... The WNV replicon RNAs were isolated from the pellet and reverse-transcribed using Prime Script™ RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCRs were performed using a SYBR PremixEX Taq II (RT)-PCR kit (Takara ...
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bioRxiv - Microbiology 2021Quote: ... The LAMP1 gene was then sub cloned into the pEF1α-mCherry-N1 vector using the In-Fusion® HD Cloning Kit (Clontech, Takara, Japan), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently cellular RNA was extracted at 6 hpi using a CellAmp Direct RNA Prep Kit (3732, Takara Bio Inc., Shiga, Japan). The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... This was replaced with a golden gate-compatible (BsaI restriction enzyme site-flanked) MCS from pICH86988 using In- Fusion cloning according to kit instructions (Takara Bio, USA) to generate a golden gate cloning-compatible version of the pK18mobsacB vector ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplified fragments were cloned into a modified pET16b expression vector (Table 2) by using In-Fusion cloning kit (Takara, Shiga, Japan). The sequence of the inserts of the resulting plasmids was verified by Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse-transcribed into cDNA using random hexamer primers and the PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time; Takara, Dalian, China). For the target genes ...
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bioRxiv - Microbiology 2020Quote: ... RNAs extracted from 100 µl of virus-containing supernatants or PBMCs were amplified by using a PrimeScript® RT reagent Kit (Perfect Real Time) (Takara Bio) and 5’RACE was performed by using a 5’RACE System for Rapid Amplification of cDNA Ends ...
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bioRxiv - Cancer Biology 2021Quote: ... and the total extracted RNA was reverse-transcribed into cDNA for polymerase chain reaction amplification using the real-time polymerase chain reaction SYBR Green kit (TaKaRa, Guangzhou, China). The reverse transcription steps were as follows ...
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimScriptTM RT reagent Kit with gDNA Eraser (TaKaRa, #RR047A; Dalian, China). Of the resultant cDNA ...
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bioRxiv - Cell Biology 2021Quote: 5’ and 3’ RACE reactions were performed to isolate full-length lncEry from the total RNA of MEP cells using the 5’- and 3’-Full RACE Kits (TaKaRa, Dalian, China) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and the RNA library was prepared with the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Takara catalog number 634861) and sequenced as described previously (29).
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bioRxiv - Cancer Biology 2022Quote: ... 0.25 ng to 10 ng of total RNA was used for cDNA library preparation using a kit suitable for RNA isolation at pico-molar concentrations (MARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian) following manufacturer recommended protocol (Clontech/Takara Bio #635005). Sequencing was performed by the UM DNA Sequencing Core ...
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bioRxiv - Cell Biology 2022Quote: ... SEPT2 constructs in pTRIP TRE Bi plasmids and all interface mutants in pTRIP TRE Bi plasmids were cloned using seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, 638910). All pcDNAs and all wild-type SEPT7 and SEPT9-containing constructs in pTRIP TRE Bi plasmids were generated with classical cloning ...
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bioRxiv - Plant Biology 2022Quote: The coding region of each P450 gene was cloned into pCAMBIA1390 vector with the In-Fusion DH Cloning Kit (TaKaRa, Kusatsu, Japan) or SLiCE reaction (Motohashi ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA (500 ng) from the worms was reverse transcribed into cDNA by using a PrimeScript RT Reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared from 2 µg of each RNA sample using the PrimeScript II 1st strand cDNA synthesis kit (TaKaRa, Shiga, Japan) with 40 units of RNasin Plus RNase inhibitor (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and a separate master mix was prepared for each primer set using the TB Green® Premix Ex Taq II (TLi RNaseH Plus) reagent kit (RR820A, Takara Bio). The final master mix contained primers at a concentration of 0.4 µM and a 1X concentration of TB Green® Premix Ex Taq II (TLi RNaseH Plus) ...
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bioRxiv - Bioengineering 2022Quote: 500 ng of total RNA extracted from cell culture were subjected to reverse transcription, using ISOGEN II (NIPPON GENE, Tokyo, Japan) and PrimeScript RT reagent Kit (TaKaRa, Kusatsu, Japan). The resulting cDNA was subjected to real-time PCR analysis using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2020Quote: For SVZ tissue libraries were prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio USA) performing 10 cycles of amplification ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... served as template in a 20 μl qRT-PCR using the TB Green Premix Ex Taq II (Tli RNaseH Plus) kit (Takara Bio Inc.) and the Roche LightCycler 96 system (Roche ...
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bioRxiv - Developmental Biology 2021Quote: SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Plant Biology 2021Quote: ... A 1 μg aliquot of RNA was used for the synthesis of the cDNA first strand using a PrimeScriptTMRT reagent Kit containing gDNA eraser (TaKaRa, Shiga, Japan). The cDNA was used as the template and primers in TableS1 were used to PCR amplify the sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... Pooled RNA from two previously identified positive NP swab samples was used for first strand cDNA synthesis (Takara primescript kit cat # 6110A). 20 μl of the cDNA reaction was diluted ten-fold and used for primary PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The chip was then loaded again on Fluidigm C1 and cDNA was synthesised and pre- amplified in the chip using Clontech SMARTer kit (Takara Clontech, Cat# 634833). In batch B ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The RNA was eluted according to the protocol in 10 uL nuclease free water and equal amounts of RNAs from different samples were immediately subjected to amplification-based cDNA synthesis using the SMARTer PCR cDNA Synthesis Kit (Takara, 634925, 639207). We have standardized the amplification cycle to 15 for 2-10 ng of initial RNA template used for the reaction ...