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Citations for Lonza :
201 - 212 of 212 citations for Recombinant Mouse PVR Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Point mutations were introduced into CJ7 WT using CRISPR RNP transfected into cells with the Amaxa mouse ES kit (Lonza VPH-1001). 1×106 cells were transfected with the RNP containing two separate guide RNAs a single stranded donor oligo and a linearized puromycin resistance gene ...
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bioRxiv - Immunology 2019Quote: ... conjugated to FITC or GFP siRNA (300 nM) conjugated to FITC two days prior to adoptive T cell therapy using the Mouse T cell Nucleofector™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
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bioRxiv - Immunology 2023Quote: ... were incubated with sgRNA and CAS9 protein complex and electroporation was done using P3 Primary Cell 4D-NucleofectorTM X Kit L (Lonza, cat # V4XP-3024) and Lonza 4D nucleofector system ...
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bioRxiv - Genomics 2021Quote: ... E14 cells were transfected with this plasmid library using a Nucleofector™ 2b device using the Mouse ES Cell Nucleofector Kit (Lonza, VAPH-1001). For each of the three biological replicates ...
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Biochemistry 2022Quote: All proteins were expressed using the baculoviral expression system and the lepidopteran High Five cell line in Insect-XPRESS (Lonza, cat. no. BELN12-730Q). Baculoviruses were produced using homologous recombination in insect cells by co-transfection with linearized baculoviral DNA and the transfer vector pAcGP67A (BD Biosciences) ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 was mixed with 500 pmol (5 μM) of 3xNLS-SpCas9-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3024) up to 25 μl in one tube ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 (see sequences of sgRNAs in Table S2) was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Neuroscience 2019Quote: ... the dissociated neurons were centrifuged down to remove the supernatant and resuspended in 80 to 100 μl of Amaxa electroporation buffer for mouse neurons (Lonza Cologne GmbH; Cologne, Germany) with mixtures of siRNA (0.2 nmol per transfection ...
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bioRxiv - Cancer Biology 2023Quote: NPE cells were transfected by electroporation using a Lonza® Nucleofector 2b device and the Lonza® Mouse Neural Stem Cell Nucleofector Kit (Lonza; #VPG-1004) according to the manufacturer’s instructions using the T-030 pulse code.
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bioRxiv - Genomics 2021Quote: ... were thawed onto a 60 mm tissue-treated culture dish seeded with irradiated mouse embryonic fibroblasts (MEFs) as feeders in DMEM high glucose base medium supplemented with 15% fetal bovine serum (FBS, Lonza cat. no. 14-501F lot no. 0000217266), 1X Pen/Strep ...