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Citations for Lonza :
101 - 150 of 212 citations for Recombinant Mouse PVR Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in Insect-XPRESS™ Protein-free Insect Cell Medium (Lonza; 12-730Q) supplemented with antibiotic-antimycotic (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were maintained in Insect-XPRESS protein-free insect cell media with L-glutamine (Lonza) and infected at a density of 3-4 x 106 with high-infectivity baculovirus for protein expression.
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bioRxiv - Bioengineering 2022Quote: Human umbilical vein endothelial cells expressing green fluorescent protein (GFP-HUVECs) (C2519A, Lonza, Basel, Switzerland) were cultured in a supplemented (EGM-2 bullet kit ...
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bioRxiv - Bioengineering 2024Quote: Human umbilical vein endothelial cells expressing green fluorescent protein (GFP-HUVECs) (C2519A, Lonza, Basel, Switzerland) were cultured in a supplemented (EGM-2 bullet kit ...
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bioRxiv - Cell Biology 2021Quote: HeLa and Mouse Embryonic Fibroblast (MEF) cells were grown in Dulbecco’s modified Eagle’s medium (Lonza) supplemented with 10% heat-inactivated fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Neuroscience 2024Quote: ... Transfections of primary cells were all performed with the Amaxa Mouse Neuron Nucleofector Kit (Lonza), using the O-005 nucleofection program ...
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bioRxiv - Immunology 2019Quote: ... the ribonucleoprotein (RNP) complex was first formed by incubating 10μg of TrueCut Cas9 Protein V2 (Lonza) with 5μg of sgRNA for 10-30min at room temperature ...
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bioRxiv - Plant Biology 2020Quote: Ubiquitinated GST-SYP61 proteins were separated by SDS-PAGE and stained with SYPRO Ruby (Lonza, Switzerland) as described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were validated with Western blot analysis using protein from Neuronal Human Astrocytes (Lonza, CC-2565) as previously reported in Knight and Serrano (2017a).
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bioRxiv - Cell Biology 2023Quote: ... were cultured at 27°C in Insect-XPRESS protein-free insect medium with L-Glutamine (Lonza) in shaking suspension ...
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bioRxiv - Bioengineering 2023Quote: ... 2×109 Freestyle CHO-S cells were resuspended in 500ml ProCHO 4 Protein-free Medium (Lonza, Cat#BEBP12-029 ...
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bioRxiv - Immunology 2023Quote: ... a ribonucleoprotein (RNP) complex was first formed by incubating 5µg of TrueCut Cas9 Protein V2 (Lonza) with 10µg of sgRNA for 10 min at room temperature ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: Mouse embryonic fibroblasts (MEFs) cells were grown in Dulbecco’s modified Eagle’s medium DMEM (12-614F, Lonza), supplemented with 5% fetal bovine serum and non-essential amino acids at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2021Quote: Xist knockdown was performed following manufacturer’s instructions for the Mouse Neural Stem Cell Nucleofector Kit (Lonza). 3-5×106 NSCs were collected and resuspended in 70 μL Nucleofector solution ...
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bioRxiv - Immunology 2019Quote: ... cells rested overnight in complete medium (human cells) or X-Vivo 15™ (mouse cells) (Lonza) were activated with IL-2 (100U/ml ...
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bioRxiv - Genetics 2021Quote: ... Transfection was carried out by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. After 2 days of transfection GFP expressing cells were FACS sorted and sparsely seeded for clonal expansion on 15cm plate ...
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bioRxiv - Genomics 2021Quote: ... using a Nucleofector™ 2b device and the Mouse ES Cell Nucleofector Kit (Lonza, VAPH-1001) according to the manufacturer’s instructions and plated into two 10 cm dishes ...
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bioRxiv - Molecular Biology 2019Quote: ... the total protein was size-separated on an 8% polyacrylamide gel and stained with SYPRO Ruby (Lonza). When the contents of the protein preparation were analyzed by western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... Sf21 cells were grown as a monolayer culture in Insect-XPRESS Protein-free Insect Cell Medium (Lonza) in standard T175 culture flasks at 27 °C ...
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bioRxiv - Neuroscience 2020Quote: The dissociated sensory neuron pellet was resuspended with 98 µL electroporation buffer (Mouse neuron nucleofector kit, Lonza) mixed with RNA oligos (0.2 nmol per transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Smarca4f/f mouse lines) or 20,000 AALE cells were plated on a 0.35% low melting agarose (Lonza) placed over a 0.7% agarose layer in a 6-well plate ...
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bioRxiv - Developmental Biology 2022Quote: ... Nucleofection experiments were performed following the manufactures’ protocol for mouse embryonic fibroblasts (Amaxa™ Nucleofector™, Lonza). To induce iFoxn1 expression ...
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bioRxiv - Neuroscience 2020Quote: ... mouse neurons were transfected before plating with 5 μg of MAP2-GFP plasmid using a Nucleofector (Lonza) according to the manufacturer’s specifications ...
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bioRxiv - Biochemistry 2022Quote: ... electroporation was carried out following the instructions of the Mouse Embryonic Stem Cell Nucleofector Kit from Lonza. After 2 days recovery ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with pcDNA3.1 encoding full-length mouse PD-1 or its mutant using nucleofection (Lonza). Transfected cells were sorted for uniform PD-1 staining and culture in medium with 0.4 cells and reporter Jurkat cells stably expressing WT or MT chimeric PD-1 (mhPD-1 ...
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bioRxiv - Biophysics 2019Quote: ... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ...
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bioRxiv - Immunology 2022Quote: ... and protein expression was carried out in suspension Trichoplusiani ni (High Five) cells grown in InsectXpress media (Lonza).
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bioRxiv - Cancer Biology 2020Quote: CRISPR/Cas9 genome editing was performed by ribonucleotide-protein (RNP) nucleofection using the 4D-Nucleofector™ System (Lonza), following the electroporation conditions already optimized in the laboratory ...
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bioRxiv - Immunology 2022Quote: ... used for baculovirus preparation were maintained in Insect-XPRESSTM Protein-free Insect Cell Medium with L-Glutamine (Lonza) at 28°C under agitation ...
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bioRxiv - Cell Biology 2023Quote: ... with a blue fluorescent protein (BFP)-tag were cultured in smooth muscle cell growth medium-2 (SMGM2) (Lonza). Human umbilical cord vein endothelial cells (HUVEC ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated by electrophoresis (ProSieve EX Running buffer 10x, diluted in H2O to 1x, (Lonza, Basel, CH); Precision Plus Protein WesternC Standards ...
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bioRxiv - Molecular Biology 2021Quote: Human and mouse fibroblasts were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (Lonza) with high glucose supplemented with 10% foetal bovine serum ...
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bioRxiv - Neuroscience 2022Quote: ... 5 × 106 trypsinized cells were resuspended in 93.5 µl solution (mouse ES cell nucleofector kit, VPH-1001, Lonza). The solution includes 2 µg of each CRISPR/Cas9 vector (4 µg total ...
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bioRxiv - Developmental Biology 2022Quote: ... and pre-seeded with low density (8,000 cells/cm2) irradiated DR4 mouse embryonic fibroblast (MEF) feeder cells (Lonza) (Matrigel-MEF) ...
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bioRxiv - Pathology 2020Quote: ... Plasmid delivery through electroporation of passage three neurospheres was performed using Mouse neural stem cell nucleofector kit (Lonza).
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bioRxiv - Cell Biology 2020Quote: ... Tln1flox/flox (control) and Tln1flox/flox Tln2−/− mouse kidney fibroblasts36 were cultured in DMEM–F12 (12-719, Lonza) with 10% FBS and 100 U/ml penicillin–streptomycin at 37°C ...
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bioRxiv - Immunology 2023Quote: Electroporation was performed to introduce siRNA into BMDCs using a mouse dendritic cell nucleofector kit (Lonza, Basel, Switzerland) and a Nucleofector 2b (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: ... MDCK II cells stably expressing these proteins were generated by transfection of 1.0×106 cells with Amaxa Nucleofector II (Lonza) using the L-005 program ...
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bioRxiv - Immunology 2019Quote: ... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
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bioRxiv - Cell Biology 2023Quote: ... RNP were formed by mixing 5 to 9 μgr base editor protein with 1.5 μgr of sgRNA in 20 μL of P3 buffer (Lonza, Amaxa P3 Primary Cell 4D-Nucleofector Kit ...
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bioRxiv - Cancer Biology 2019Quote: ... NSCs were harvested with Accutase and 2-3×106 cells were resuspended in 100µl mouse neural stem cell nucleofector buffer (Lonza) with 2µg plasmid DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... pPBCAG-hph and a PiggyBac Transposase vector using the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001). Transfected cells were selected with 200 μg/ml hygromycin B Gold (Ibian tech. ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×106 EL16.7 TST ESCs were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001) using program A-30 with 1.6 µg each of GFP and tdTomato circularised targeting vectors and 5 µg single gRNA vector PX459 (5’-TATACCTAATCATTATGCCG-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... The siRNA transfections for primary BMDMs and pancreatic fibroblasts were performed using the Mouse Macrophage Nucleofector™ Kit (Lonza) and Nucleofector™ 2b Device (Lonza ...
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bioRxiv - Biochemistry 2021Quote: ... or ds-UM DNA oligos (IDT) (Table S4) were nucleofected into mouse ES cells using an Amaxa nucleofector (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... For each nucleofection 3 x 106 cells were resuspended in 80 μl of mouse ES cell nucleofection solution (Lonza). Equimolar (0.32 nmol ...
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bioRxiv - Molecular Biology 2019Quote: ... and wild-type J1 male mouse embryonic stem cells were grown on 0.1% gelatin-coated dishes in DMEM (Lonza) complemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were immortalized with pBabe-SV40Tag by electroporation using the Amaxa Mouse/Rat Hepatocyte Nucleofector Kit (Lonza, VPL-1004). Immortalized MEF lines were genotyped ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CCL206 lung fibroblasts (CCL-206; ATCC, Wesel, Germany, RRID: CVCL_0437) were cultured in DMEM (Lonza, Basel, Switzerland, #42430):Ham’s F12 (Gibco ...