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Citations for Lonza :
601 - 650 of 763 citations for Recombinant HBV X Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Synthetic Biology 2021Quote: ... and γδ T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 medium (Lonza) (5% fetal bovine serum ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to the well of a 16-well Nucleocuvette™ strip (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were electroporated on a 4D-NucleofectorTM X Unit (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... Feng and Coulombe 2015b) were transfected into Krt14-/- skin keratinocytes using P1 Primary Cell 4D-Nucleofector™ X Kit (V4XP-1024, Lonza). After nucleofection ...
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bioRxiv - Cell Biology 2019Quote: ... was transfected into skin keratinocytes in primary culture using the P1 Primary Cell 4D-Nucleofector™ X Kit (V4XP-1024, Lonza).
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bioRxiv - Neuroscience 2022Quote: ... After four days 12 EBs were transferred into each well of 6 well plate in X-VIVO 15 (Lonza, BE02-060Q) containing 100 ng/mL M-CSF (Peprotech ...
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bioRxiv - Genomics 2021Quote: ... Four aliquots of one million cells each per study subject were stimulated overnight for 12 hours in 1 ml X-VIVO™ 15 Serum-free Hematopoietic Cell Medium (Lonza) with 10 ul ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... pcDNA3-JUP or empty vector were transfected into Caco-2 cells using SE Cell Line 4D-Nucleofector™ X Kit (Lonza) and an Amaxa 4D Nucleofector device from Lonza ...
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bioRxiv - Bioengineering 2020Quote: 2 million UCB-MNC/mL were cultured in X-VIVO 15 serum-free cell-culture medium (Lonza Group Ltd, Basel, Switzerland), supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected with the enzyme and with PAK1 or PAK2 sgRNA using nucleofection (4D-Nucleofector™ X Unit, Lonza). Due to low transfection efficiency in leukemia cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Monocytes were isolated by adherence to plastic and then cultured for one week in X-VIVO 15 serum-free medium (Lonza, Inc.) and 20 ng / mL recombinant human GM-CSF (300-03 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended with 10µL of primary cell nucleofection solution (P3 primary Cell 4D-Nucleofector X kit S (Lonza, # V4XP-3032), and mixed with RNP complexes ...
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bioRxiv - Genomics 2022Quote: mESC were transfected with sgRNA constructs using the P3 Primary Cell 4D-Nucleofector X Kit (V4XP-3024) and the Amaxa 4D Nucleofector™ system (Lonza). We used the transfection programme CG-104 ...
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bioRxiv - Immunology 2022Quote: ... were delivered into the CD4+ Human T cells by using Amaxa 4D-Nucleofector System and P2 Primary Cell 4D-Nucleofector® X Kit S (Lonza) according to manufacturer’s instructions using the pulse code EH100 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 10 µL of transduced cells were mixed with 90 µL of SF buffer and ZFN mRNA and electroporated using a 4D-X nucleofector using pulse code FF-120 per manufacturer’s recommendation (Lonza, Basel, Switzerland). Cells received 1.6 µg of CCR5-specific ZFN mRNA or 0.7 µg of each AAVS1-specific ZFN mRNA.
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bioRxiv - Biochemistry 2019Quote: ... 1.5-2×106 cells were pelleted for each condition and resuspended in 100 μL complete nucleofector solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza) to which 1μg of pCR2.1-mClover-LMNAdonor ...
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bioRxiv - Developmental Biology 2020Quote: ... or a PMO Control at 100 µM in 100 µL solution from the P3 Primary Cell 4D-Nucleofector® X Kit L (V4XP-3024, Lonza) using the CB150 program on the 4D-Nucleofector™ System (Lonza) ...
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bioRxiv - Neuroscience 2019Quote: ... or a non-silencing plasmid immediately before plating (Amaxa™ P3 Primary Cell 4D-Nucleofector X Kit L; Lonza, CU133 program). The generation and knockdown efficiencies of shRNA plasmids were described previously (Guner et al. ...
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bioRxiv - Immunology 2021Quote: ... On the opposite chamber, 2.7 x 105 / cm2 of NCI-H441 (ATCC, Manassas, USA) were cultured in RPMI-1640 (Lonza, Cologne, Germany) supplemented with 10 % FCS ...
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bioRxiv - Pathology 2021Quote: ... the wires were removed and the through-channels were filled with tissue culture media (1:1 mixture of X-Vivo15 and Bronchial Epithelial Growth media (Lonza, USA) with antibiotics (MP Biomedicals ...
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bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
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bioRxiv - Genetics 2021Quote: Human K562 cells were nucleofected with already complexed RNP using the SF Cell Line 4D Nucleofector X Kit for Amaxa 4D device (Lonza, Basel) with program FF-120 ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcribed mRNAs encoding the TCR α- and β-chains were mixed at a 1:1 ratio and electroporated in activated T cells on a Lonza 4D-NucleofectorTM device using the P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza) according to the recommendations of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... collected by centrifugation at 500xg for 5 min and resuspended in SF solution (SF cell line 4D-Nucleofector™ X Kit, Lonza). After addition of the RNP ...
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bioRxiv - Immunology 2022Quote: ... 10e6 T cells per screening condition were transferred to one T75 flask in 20 ml of X-VIVO 15 (Lonza Bioscience) supplemented with 5% FCS ...
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bioRxiv - Immunology 2023Quote: ... transferred into a Nucleocuvette and immediately electroporated using the CL120 program in the 4D-Nucleofector X Unit (Lonza, Walkersville, MD, USA). After electroporation ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection in the appropriate cell lines was achieved by nucleofection using program X-014 of the AMAXA Nucleofector® apparatus (Lonza) as previously described (Burkard et al. ...
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bioRxiv - Genetics 2023Quote: ... The pellet was washed with 10 ml of 1 x PBS and resuspended in 20 µl of nucleofection SF buffer (Lonza Biosciences), in which 600 ng of the DsRed2-N1 plasmid was added to the suspension ...
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bioRxiv - Cell Biology 2023Quote: ... brucei 13-90 cells were electroporated with 10 µg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (program X-001) (Lonza, Switzerland).
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bioRxiv - Immunology 2024Quote: ... Jurkat T cells were transfected with ON-TARGETplus SMARTpool human Piezo1 siRNA or control siRNA (Horizon Discovery, previously Dharmacon) with SE Cell Line 4D-Nucleofector™ X Kit (Lonza). Jurkat T cells were used for the experiment after 24 h.
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bioRxiv - Immunology 2023Quote: ... Electroporation was performed with sgRNA/Cas9 RNP complexes using Alt-R Sp Cas9 Nuclease V3 (IDT) and using the 4D-Nucleofector with P3 Primary Cell 4D Nucleofector X Kit S (Lonza Bioscience).
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bioRxiv - Immunology 2023Quote: ... Cells enriched by Percoll density-dependent sedimentation were adjusted to a concentration of 1 × 106 cells/ml in serum-free X-VIVOTM 15 medium (Lonza BioWhittaker) containing 100 ng/ml recombinant human SCF (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... and a control siRNA (D-001810-01-20 ON-TARGET plus Nontargeting siRNA #1) were prepared for electroporation using the Lonza SF Cell Line 4D-Nucleofector™ X Kit (V4XC-2012; Lonza) according to the manufacturer’s instruction with minor alterations ...
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bioRxiv - Immunology 2024Quote: ... 5×104 APC were seeded together with 1×105 CD4+ T cells in the presence of relevant peptides at 2.5 ng/ul diluted in X-VIVO 15 medium (Lonza, Basel, Switzerland) supplemented with 4 % human AB plasma (Innovative Research ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1e6 cells were loaded across two different nucleocuvettes with SE reagent and electroporated under program CL-120 on a 4D-Nucleofector X Unit (Lonza, NC0475784). Following puromycin selection at 2µg mL-1 (InvivoGen ...
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bioRxiv - Cell Biology 2023Quote: ... S2Xpress cells were cultured in Insect-XPRESSTM protein-free medium (LONZA, Belgium) supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin (GIBCO) ...
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bioRxiv - Immunology 2023Quote: ... Protein expression was induced with 5µM CdCl2 in Insect-XPRESS medium (Lonza) for 5-7 days and supernatants were concentrated and purified using strep-tacting affinity chromatography columns in an AKTA FPLC system ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Electroporation of K562 cells was performed using a SF Cell Line 4D-Nucleofector kit and 4D-X Nucleofector using pulse code FF-120 (Lonza, Basel, Switzerland), per the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... using the AMAXA Nucleofector 4D system (U-033 setting) and the P3 primary cell 4D-nucleofector X kit with the 16-well nucleocuvette strip (Lonza, V4XP-3032). Clones were obtained by FACS using the FACS Aria II sorter at the Stanford Shared FACS Facility for the brightest red parasites and single cloning the TdTomato+ enriched population by limiting dilution into 96-well plates.
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bioRxiv - Bioengineering 2019Quote: Nucleofection of the HDR template and CRISPR-Cas9 vectors was performed with SF Cell Line 4D-Nucleofection X Kit L (Lonza, V4XC-2024). Before nucleofection hybridoma cells were assessed for viability and centrifuged (90g ...
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bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
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bioRxiv - Systems Biology 2020Quote: Single-cell suspensions were pelleted at 400 x g for 5 min and washed once with 10 mL mammary epithelial basal medium (MEBM; Lonza CC-3151). For each sample ...
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bioRxiv - Immunology 2021Quote: ... hematopoietic progenitors were harvested and the hemogenic endothelium left behind in the 12-well plate was cultured in X-VIVO15 (Lonza, Basel, Switzerland) supplemented with β-mercaptoethanol (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... then transferred to a 15mL conical tube with 8mL pre-warmed Xeno-Free Neural Stem Cell Media (XF-NSM) consisting of X-Vivo Basal Medium (Lonza, 04-744Q), 1X N2 Supplement (Invitrogen ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... complex with a ratio of 4.5 to 1 between sgRNA and Cas9 was delivered following the protocol of the SE Cell Line 4D-NucleofectorTM X Kit (Lonza, V4XC-1012), using the nucleofection program DS-130 on the Lonza 4D X unit ...
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bioRxiv - Neuroscience 2020Quote: ... hESCs human cortical neurons were nucleofected with shRNAs constructs against ASH1L using the X-unit of the Amaxa nucleofector unit (Lonza, catalog # AAF1002X). Human neurons between days 30 to 32 of neuronal induction were single cell dissociated using single cell dissociation reagent (stem cell technologies CT catalog # 07174 ...
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bioRxiv - Cell Biology 2021Quote: ... subjected to red blood cell lysis with ACK lysis buffer and electroporated with P3 Primary Cell 4D-NucleaofactorTM X -kit (V4XP-3032, Lonza, Basel, Switzerland), as previously described (Freund et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... AsPC-1 and CAPAN-1 pellets were suspended in Lonza transfection reagent (SE Cell Line 4D-Nucleofector™ X Kit L, Lonza, Germany) when the Mirus transfection reagent was finished ...
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bioRxiv - Immunology 2020Quote: ... All isolated Tregs were activated by plate bound anti-CD3 and anti-CD28 antibodies and cultured with X-VIVO 20 media (LONZA #04-448Q) supplemented by 1X Pen/Strep ...
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bioRxiv - Immunology 2019Quote: ... as a control two days prior to adoptive T cell therapy using the P3 Primary Cell 4D-Nucleofector X™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.