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Citations for Lonza :
501 - 550 of 763 citations for Recombinant HBV X Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... 200,000 cells were electroporated with 1000 ng adRNA plasmid or 48 pmol of IVT RNA using the Amaxa SF cell Line 4D-Nucleofector X kit (Lonza) as per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... EBs were collected and used to setup macrophage factories in T75 flasks (75 EBs/flask) or T175 flasks (150 EBs/flask) in X-Vivo15 (Lonza), supplemented with 100 ng/mL M-CSF (PeproTech) ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was transferred to an electroporation cuvette and electroporated using program X-001 on an Amaxa Nucleofector II (Lonza). Cells in the cuvette were mixed with 300 μl of DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... The efficacy of siRNA targeting Kdm6a was assessed by electroporation of neurons using a 4D-Nucleofector X Unit and the corresponding P3 Primary Cell nucleofection kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... transferred to a 100 μl nucleocuvette and pulsed using the program FF-137 on an Amaxa 4D-Nucleofector X Unit (Lonza). Cells were then resuspended in equilibrated ...
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bioRxiv - Genetics 2020Quote: ... The cell suspension was gently mixed with 2.5µL/reaction of appropriate RNP and then pipetted into a 96-well-format nucleofection cuvette for the Lonza 4D X unit or Shuttle unit (Lonza). Except where explicitly stated ...
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bioRxiv - Microbiology 2021Quote: ... Both resuspensions were mixed in a 100 μl cuvette (P3 Primary cells 4D-Nucleofector X kit L, V4XP-3024, Lonza). The programme FI-158 was used for electroporation ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Cell Biology 2022Quote: ... were nucleofected with either PGK-mO-Dam or PGK-mO-Dam-Zfp266 plasmids using the P4 Primary Cell 4D-Nucleofector X Kit (Lonza). 5 replicates were performed in total ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 NSCs were mixed with 5 µg of corresponding DNA with the Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) and pulse was delivered using the CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... 2×105 NSCs for essential gene expression were nucleofected with 750 ng each of the above-mentioned plasmids using SG Cell Line 4DNucleofector X Kit (Lonza), DN-100 program ...
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bioRxiv - Cell Biology 2022Quote: A GFP sgRNA vector was delivered into Cas9 and GFP expressing NSCs using nucleofection with the SG Cell Line 4DNucleofector X Kit (Lonza) as per manufacturer’s instructions51 ...
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bioRxiv - Microbiology 2022Quote: ... Ribonucleoproteins were introduced into 1 × 106 sub-confluent BlaER1 cells using 4D-Nucleofector X Unit and SF Cell line Kit (Lonza), applying program DN-100 ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of the pSAG1::Cas9-U6::sgUPRT vector15 and 10 μg of the barcode oligo (equivalent to an ~1:160 molar ratio of plasmid to oligo) were co-transfected into approximately 1×106 extracellular tachyzoites using the 4D-Nucleofector X Unit programme F1-115 (Lonza). 24 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: ... The electroporation was done using a 4D nucleofector (4D-Nucleofector Core Unit: Lonza, AAF-1002B; 4D-Nucleofector X Unit: AAF-1002X; Lonza), with CM137 as the pulse program and P3 as the selected electroporation buffer ...
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bioRxiv - Immunology 2022Quote: ... Twenty μl of cells in buffer were transferred to each well of a 16-well Nucleofection cuvette strip (4D-Nucleofector X kit S; Lonza). Three μl of RNP were gently dispensed to each well ...
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bioRxiv - Immunology 2022Quote: ... activated-DC were washed twice in PBS 1X and put in culture with allogeneic naive CD4+ T cells (104 DC and 5×104 T) in X-VIVO 15 media (LONZA) for the indicated time ...
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bioRxiv - Cell Biology 2022Quote: ... we used Cas9-RNP and the Lonza 4D-Nucleofector System together with the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza) to transfect and create Ndrg2-KO DCs ...
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bioRxiv - Cell Biology 2022Quote: ... For nucleofection we used the 4D-Nucleofector System and P3 Primary Cell 4D Nucleofector X Kit L (Lonza, Basel, Switzerland). We optimized the RNP electroporation for DCs by using multiple-guide mixtures and different number of cells in a 100uL total volume ...
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bioRxiv - Immunology 2020Quote: ... Rinsed cells were resuspended in buffer P4 (mouse) or P2 (human) at 1-10 x 106 cells/20 μL (Lonza). Non-targeting Alt-R crRNA #1 ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were activated with plate-bound α-CD3 (3.75 µg/ml; Immunotech) and soluble α-CD28 (1 μg/mL; Immunotech) in X-vivo 20 serum-free medium (Lonza). X-vivo 20 medium was supplemented with L-glutamine (2 mM ...
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bioRxiv - Microbiology 2020Quote: ... 20 µL of cell suspension was then gently mixed with each crRNP and aliquoted into a 96-well electroporation cuvette for nucleofection with the 4-D Nucleofector X-Unit (Lonza) using pulse code EO-120 ...
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bioRxiv - Cancer Biology 2020Quote: ... were derived by isolation of Lin-BM HSPCs from double mutant mice and were cultured in X-Vivo medium (Lonza) in the presence of 10 ng/mL mIL-3 ...
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bioRxiv - Immunology 2021Quote: ... Cells were plated at 2 x 106 cells and cultured in RPMI with 10% FCS with low endotoxin levels (Lonza). After 14 d ...
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bioRxiv - Immunology 2022Quote: ... 1*106 purified naïve T-cells were resuspended in 15 μL buffer P3 + 5 μL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7 μL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Microbiology 2022Quote: ... transfections were performed by introducing DNA into ∼108 Percoll-enriched schizonts by electroporation using an Amaxa 4D Nucleofector X (Lonza), using program FP158 as previously described (Moon et al. ...
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bioRxiv - Genetics 2022Quote: ... Libraries were electroporated into K562 cells in 100ul volumes using the Amaxa system (Program X-001, Neucleofactor kit V, Lonza). We performed 6 independent replicates with each replicate consisting of ∼2×108 cells ...
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bioRxiv - Immunology 2022Quote: ... 2 million B cells were then washed with PBS and resuspended in 15 uL resuspension buffer (4D-Nuclcofector X Kit S, #V4XP-4032; Lonza). Cells in resuspension buffer (12.5ul ...
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bioRxiv - Immunology 2022Quote: ... was used to enrich in CD4+ or CD8+ T cells from PBMCs that were cultured in X-vivo 15 media (Lonza) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Tightly synchronized schizonts were transfected using the Amaxa 4-D electroporator and P3 Primary Cell 4D Nucleofector X Kit L (Lonza) according to the protocol described by Moon et al ...
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bioRxiv - Immunology 2019Quote: ... were generated by seeding 2 x 106 BM cells in a 100 mm non-tissue culture treated dish in RPMI 1640 (Lonza) supplemented with 10% FCS ...
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bioRxiv - Genetics 2020Quote: ... 2×105 UE control hPSCs were transfected with a pair of pSpCas9(BB)-2A-GFP constructs containing the specific sgRNAs (350 ng per construct) using P3 Primary Cell 4D-Nuclecfector X Kit (Lonza). The transfected cells were plated in Matrigel-coated dish and cultured for 2 days ...
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bioRxiv - Immunology 2021Quote: ... 2.106 T cells were resuspended in 20 µl of nucleofection solution with 3 µl or 4 µl RNP and transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Cell Biology 2019Quote: ... 2 x 106 cells of MEFs were transfected with 1.2 nM of siRNAs using Amaxa Nucleofector II (Lonza, Basel, Switzerland) and MF 1 Nucleofector kit (Lonza ...
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bioRxiv - Genetics 2021Quote: ... were transfected with either KMT2D CRISPR-Cas9 vector or CRISPR-Cas9 empty vector together with GFP plasmid using P3 Primary Cell 4D-Nucleofector X kit (V4XP-3024, Lonza). Three days after transfection ...
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bioRxiv - Immunology 2021Quote: ... Cells were then nucleofected using a 4D-Nucleofector™ with X-Unit (V4XP-4032 and V4XP-9096, Lonza, Basel, Switzerland). Up to three RNP complexes in 9 µL were used per reaction ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T-cells were stimulated with plate-bound α-CD3 (3.75 μg/ml; Immunotech) and soluble α-CD28 (1 μg/mL; Immunotech) in X-vivo 20 serum-free medium (Lonza). X-vivo 20 medium was supplemented with L-glutamine (2 mM ...
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bioRxiv - Genomics 2020Quote: ... bulk PBMCs obtained from healthy donors were thawed and cultured in a T-cell growth media (TCGM) consisting of X-VIVO15 (Lonza) supplemented with 5% human serum ...
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (500 ng/mL) at a density of 2 × 106 cells/mL of X-vivo 15 serum-free medium (Lonza). For iTreg differentiation ...
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bioRxiv - Immunology 2020Quote: ... or media were cultured in 96 well flat bottom plates overnight then PBMC (2×105) from healthy donors were cultured at 37 °C in X-VIVO 15 media (Lonza) with 50-200 U/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2021Quote: ... were transfected with 500 ng plasmid encoding pAcGFP-fused CDC42 variants or hMEFV-pAcGFP1-t2a-CDC42 using 4D-Nucleofector and the SG Cell Line 4D-Nucleofector X Kit (Lonza). Immediately after nucleofection ...
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bioRxiv - Neuroscience 2022Quote: ... and the spheres were collected by inverting the strainer into a new conical tube and rinsing with hematopoietic cell media consisting of X-VIVO 15 (Lonza) supplemented with 1X Pen/Strep (Thermo-Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... The transfected cells were then co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) and cultured until virus-mediated CPE was observed ...
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bioRxiv - Bioengineering 2022Quote: ... 90 μL of nucleofection solution (16.2 μL of Supplement solution mixed with 73.8 μL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza) was mixed thoroughly with the cell pellet ...
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bioRxiv - Cell Biology 2022Quote: ... and 20 μg of the donor DNA using the Amaxa 4-D Nucleofactor X machine (Pulse code FP158) and P3 Primary Cell Kit (Lonza), according to protocols described by Moon et al ...
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bioRxiv - Immunology 2022Quote: ... we used SF Cell Line 4D-Nucleofector™ X Kit L (for Raw 264.7 cells) or SG Cell Line 4D-NucleofectorTM X Kit L (for NIH/3T3 cells; both from Lonza Inc.). Up to 5 × 106 cells were resuspended in 100 μl of nucleofection solution ...
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bioRxiv - Immunology 2022Quote: ... containing RNP complex and enhancer DNA (IDT, Cat.1075915) and under electroporation with EO-115 program of 4D-nucleofactor X Unit (Lonza). Electroporated T cells recovered overnight in IL7 and Il15 medium were then activated by anti-human CD3/CD28 beads (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: Macrophage culture was performed under entirely serum-free conditions throughout using X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) supplemented with 1% penicillin-streptomycin (Cat ...
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bioRxiv - Microbiology 2022Quote: ... was added to the resuspended cells and nucleofected using the CM-150 program on the 4D-Nucleofector X Unit (Lonza). Nucleofected cells were allowed to recover in a 37°C incubator with 5% CO2 for 20 minutes ...