Labshake search
Citations for Lonza :
401 - 450 of 500 citations for IL 10 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). Once cells reached 80% confluency ...
-
bioRxiv - Genomics 2021Quote: ... E14 cells were transfected with this plasmid library using a Nucleofector™ 2b device using the Mouse ES Cell Nucleofector Kit (Lonza, VAPH-1001). For each of the three biological replicates ...
-
bioRxiv - Cell Biology 2021Quote: ... Marrow cells were resuspended in 10 mL α – MEM and subjected to density centrifugation using 20 mL Lymphoprep™ (Lonza) at 800 x g for 20 minutes with no braking on the centrifuge ...
-
bioRxiv - Cell Biology 2022Quote: hTERT immortalized RPE-1 (RPE1) cell lines were grown in an 1:1 mix of DMEM and F-10 (Lonza) and Human Embryonic Kidney (HEK ...
-
bioRxiv - Cell Biology 2022Quote: ... peeled renal tubular epithelial cells were cultured in Primary medium (DMEM/F12, 10% FBS, 100U/mL penicillin, 100mg/mL streptomycin, amphotericinB, 1×REGM SingleQuots (CC-4127, Lonza)) in 6-well plates ...
-
bioRxiv - Genomics 2020Quote: LNCAP (ATCC) and PC3 (ATCC) cells were cultured and maintained in RPMI media supplemented with 10% fetal bovine serum (FBS; Lonza) 1mM sodium pyruvate (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... 3 x 107 bloodstream form cells were harvested by centrifugation and transfected with 5-10 μg of linearized plasmid DNA using an Amaxa Nucleofector II (Lonza) with program X-001 ...
-
bioRxiv - Molecular Biology 2020Quote: ... CD34+ cells were purified from the apheresis PBMC collection via immunoabsorption and cultured overnight in at 37°C in X-VIVOTM 10 (Lonza) supplemented with 1% HSA (Baxter ...
-
bioRxiv - Physiology 2019Quote: ... 10% AIM-V and 100 units/ml penicillin/ streptomycin and transiently transfected using Nucleofector™ Kits for Human Melanocytes (Lonza) or magnetofection with PolyMag Neo magnetic beads (OZ Biosciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% FBS (Biowest, Nuaillé, France) and 50 μg/ml penicillin and 50 μg/ml streptomycin (Lonza, Basel, Switzerland). Cells were maintained at 37°C and 5% CO2 in a humidified incubator.
-
bioRxiv - Biochemistry 2019Quote: ... 3×107 cells were transfected with 10 μg of NotI linearized plasmids using the AMAXA electroporator (Lonza, program X-001) as described [14] ...
-
bioRxiv - Cancer Biology 2019Quote: ... A20 cells were grown in RPMI 1640 medium (whereas all other cells were grown in DMEM) supplemented with 10% (v/v) FBS (Lonza) and with Pen/Strep Amphotericin B (Lonza ...
-
bioRxiv - Immunology 2021Quote: ... The remaining tissue was minced and digested with stirring for 60 min in complete RPMI (RPMI1640; Lonza; supplemented with 10% fetal calf serum (FCS, 1% Pen/Strep; Lonza) containing 0.25 mg/ml Liberase TL (Roche ...
-
bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
-
bioRxiv - Biochemistry 2020Quote: ... COS7 cells were cultured in DMEM with 4.5g/l glucose supplemented with 10% DMEM and antibiotics (Lonza Biologics, Slough, UK). transiently transfected with expression plasmids using TransIT-2020 (Mirus ...
-
bioRxiv - Microbiology 2020Quote: ... Homogenate was resuspended with 6 ml per brain of prewarmed complete media (DMEM [Corning]; 10% FBS; 1% Nonessential Amino Acids Mixture 100x, [Biowhittaker Reagents Lonza],1% GlutaMAX supplement 100x [Thermo Fisher Scientific] ...
-
bioRxiv - Microbiology 2020Quote: ... fumigatus collected from three 10-cm plates grown for 7 days at 37°C were germinated with shaking in RPMI 1640 (Lonza) liquid media supplemented with glucose to a final concentration of 2% (w/v ...
-
bioRxiv - Cancer Biology 2022Quote: ... Pmel-1 CD8+ T cells were activated by incubating with R10 media (RPMI containing 10 % FBS, 100 U/mL penicillin and 100 mg/mL streptomycin (all Lonza), 1x MEM NEAA ...
-
bioRxiv - Immunology 2021Quote: ... Cells were plated at 2 x 106 cells and cultured in RPMI with 10% FCS with low endotoxin levels (Lonza). After 14 d ...
-
bioRxiv - Immunology 2019Quote: ... The superior right lung lobe was mashed through a cell strainer and treated with Ammonium-Chloride-Potassium (ACK) lysis buffer (10-5483, Lonza). Cells were pelleted ...
-
bioRxiv - Molecular Biology 2020Quote: ... Freshly isolated HBECs were plated onto rat tail collagen type I-coated (10 μg/ml) dishes in Bronchial Epithelial Growth Medium (BEGM, Lonza) containing 1% antibiotic– antimycotic solution (AA ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Successful test compounds from the mf assay were diluted to 10 μM and added to the trans-wells in 6 ml endothelial basal media with supplements (EGM-2 MV; Lonza). Twelve replicates (n = 6 wells ...
-
bioRxiv - Molecular Biology 2021Quote: 3T3-L1 fibroblasts were maintained in DMEM supplemented with 10% newborn calf serum (NCS) and 1% penicillin/streptomycin (P/S) (all Lonza). Experiments were performed in six-well plates ...
-
bioRxiv - Cancer Biology 2020Quote: ... murine 3B11 and human HUVECs endothelial cells (all purchased from ATCC) were cultured in RPMI 1640+ 10% Fetal Bovine Serum (FBS) or in endothelial grown media (EGM-2-Lonza).
-
bioRxiv - Immunology 2020Quote: ... The tissue was cut into small pieces and then processed in digestion buffer containing HANKS Medium (10-527F, Lonza, UK), 608 U/ml of Collagenase I (C9891m Sigma ...
-
bioRxiv - Immunology 2020Quote: ... and 5×106 cells seeded in 100-cm2 cell culture dishes in 12 mL RPMI supplemented with 10% fetal calf serum (Lonza), 1X non-essential amino acids (NEEA ...
-
bioRxiv - Immunology 2021Quote: Spleens and cervical lymph nodes were isolated from 6-10 weeks old C57B6 mice and homogenized through a sieve in B cell culture medium (RPMI-1640 (Lonza), 10% fetal calf serum (FCS ...
-
bioRxiv - Biophysics 2022Quote: ... The transfected cells were then co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) and cultured until virus-mediated CPE was observed ...
-
bioRxiv - Bioengineering 2022Quote: ... Bone marrow mononuclear cells were plated in endothelial growth medium 2 + 10% fetal bovine serum (FBS) (EGM-2MV, Lonza, Switzerland) on fibronectin coated plates and cultured at 37°C in 5% CO2 incubators ...
-
bioRxiv - Immunology 2022Quote: Macrophage culture was performed under entirely serum-free conditions throughout using X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) supplemented with 1% penicillin-streptomycin (Cat ...
-
bioRxiv - Microbiology 2022Quote: ... Both HPMEC and HUVEC cell lines were propagated (passages 5–10) and maintained in endothelial growth medium 2 (EGM-2) using the EGM-2 bullet kit from Lonza following the manufacturer’s specifications and grown in a CO2 incubator at 37°C with 5% CO2.
-
bioRxiv - Microbiology 2022Quote: ... ID) were excysted and transfected with 10 ug each of DiCre and floxed plasmids using an Amaxa 4D Electroporator (Lonza). The transfected parasites were diluted in HCT-8 infection medium and divided equally to infect three 24 well plates of sub-confluent HCT-8 cells ...
-
bioRxiv - Microbiology 2023Quote: ... 190 μL was mixed with 10 μL of 20X SYBER Green I nucleic acid stain (Lonza Walkersville Inc, Walkersville, MD) in a well of a transparent 96-well plate and incubated in the dark for 15 minutes before being placed in the Guava easyCyte HT flow cytometer (488 nm ...
-
bioRxiv - Immunology 2022Quote: Sorted CD8+ T cells with low and high ΔYm were centrifuged at 200 × g for 10 minutes prior to nucleofection (4D nucleofector system, Lonza). Three-five million cells were combined with 200 pMol siRNA (siControl or siME1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the paired RNPs were combined and nucleofected into 4×105 Raw 264-7 cells suspended in 10 ul of nucleofection buffer (Lonza) using the program DS-136 in a 4D Nucleofector X Unit (Lonza) ...
-
bioRxiv - Genomics 2023Quote: ... Neurons were nucleofected with 10 µ total DNA plasmid DNA using the AD1 4D-Nucleofector Y kit (Lonza V4YP-1A24) with the ED158 (iCell GlutaNeurons ...
-
bioRxiv - Bioengineering 2023Quote: Ha deposition was detected staining constructs sections (n≥10 from n≥3 chambers considering two different hACs and two different MSCs donors) with OsteoImage (Lonza) as detailed above ...
-
bioRxiv - Genomics 2023Quote: ... 5 µg of the 4sgRNA plasmid or 10 µM synthetic guide RNAs were mixed and the cell/reagent/nucleofection mix was transferred to Nucleofection cuvette strips (Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
-
bioRxiv - Cell Biology 2023Quote: ... hydrogels were prepared by diluting collagen I stock to a final concentration of 2.5 mg/mL with 10% v/v 10x MEM-α (BE12-684F, Lonza), 10% v/v foetal bovine serum ...
-
bioRxiv - Immunology 2023Quote: ... Mixtures of complexed sgRNA and Cas9 (0.3 nmol synthetic sgRNA + 62 µmol Cas9 nuclease) and 2-10×106 enriched murine CD8+ T cells were suspended in 25ul of P3 electroporation buffer (Lonza) and electroporated using the Lonza 4D Nucleofector (pulse code DN100) ...
-
bioRxiv - Bioengineering 2023Quote: NIH/3T3 fibroblasts were cultured at 37 °C and 5% CO2 in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (Lonza, Basel, Switzerland) and 1% penicillin/streptomycin antibiotic ...
-
bioRxiv - Neuroscience 2024Quote: ... 2.5 × 10 5 phNPCs cells were used per electroporation reaction in one cuvette of the 16-well Nucleocuvette Stripe (Lonza). After trypsinization ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were passaged 1:10 every 3-4 days by trypsinisation and were regularly screened for mycoplasma contamination (Mycoalert, Lonza).
-
bioRxiv - Cancer Biology 2024Quote: ... by flushing one femur with PBS 2% FBS followed by erythrocyte elimination with ACK Lysing Buffer (10-548E, BioWhittaker, Lonza) (Figure 4BC) ...
-
bioRxiv - Neuroscience 2019Quote: ... the dissociated neurons were centrifuged down to remove the supernatant and resuspended in 80 to 100 μl of Amaxa electroporation buffer for mouse neurons (Lonza Cologne GmbH; Cologne, Germany) with mixtures of siRNA (0.2 nmol per transfection ...
-
bioRxiv - Cancer Biology 2023Quote: NPE cells were transfected by electroporation using a Lonza® Nucleofector 2b device and the Lonza® Mouse Neural Stem Cell Nucleofector Kit (Lonza; #VPG-1004) according to the manufacturer’s instructions using the T-030 pulse code.
-
bioRxiv - Cell Biology 2021Quote: ... containing bone marrow mononuclear cells was washed in 5 ml basal medium (α – MEM containing 10% FBS and 100 U mL−1 penicillin and 100 μg mL−1 streptomycin; Lonza). All washing stages were at 400 x g for 5 minutes.
-
bioRxiv - Cell Biology 2020Quote: ... containing bone marrow mononuclear cells was washed in basal medium (α-MEM containing 10% FBS and 100 U mL-1 penicillin and 100 µg mL-1 streptomycin; Lonza). Cells were subsequently either sorted using MACS or incubated with SNAs.
-
bioRxiv - Microbiology 2019Quote: ... 10 μL were loaded onto 10 % polyacrylamide gels and separated in Tris-Tricine buffer according to manufacturer’s instructions (ProSieve; Lonza, Basel, Switzerland). The gels were wet blotted (Mini Trans-Blot Cell ...
-
bioRxiv - Systems Biology 2021Quote: ... complemented with 10% fetal bovine serum (Biosera, France) and Penicillin/Streptomycin (100 U/ml and 100 μg/ml, respectively - Lonza, Switzerland). Cells were seeded on poly-L-lysine pretreated (0.001% ...