Labshake search
Citations for Lonza :
451 - 500 of 500 citations for IL 10 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... the media was changed to α-MEM containing macrophage colony-stimulating factor (M-CSF; 10 ng/ml) and and 66ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL, Lonza Biosciences). OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% of heat-inactivated fetal bovine serum (Biowest™) and 1% of antibiotics (penicillin + streptomycin, Lonza™ BioWhittaker™). One day after plating ...
-
bioRxiv - Microbiology 2021Quote: ... at passage 53 was grown in Dulbecco’s Modified Eagles Medium (DMEM) supplemented with 10% (v/v) heat inactivated foetal bovine serum (FBS; Lonza, Basel, Switzerland), 1× Non Essential Amino Acids (NEAA) ...
-
bioRxiv - Immunology 2021Quote: ... Buffy coat cells were washed three times in 2.5 mM EDTA-PBS at 4°C before dilution and maintenance in RPMI-1640 supplemented with 10% FCS (PAA, AT or Lonza, CH) 2 mM glutamine ...
-
bioRxiv - Bioengineering 2022Quote: ... Deep Blue was prepared at a ratio of 1:10 with colourless DMEM (without pyruvate, with 4.5 g/L glucose (Lonza, Basel, Switzerland)) and was added to each existing well and incubated at 37 °C for 3 h ...
-
bioRxiv - Cancer Biology 2022Quote: Whole peripheral blood samples from either healthy donors or AML patients were processed to obtain mononuclear cells (PBMCs) through density gradient centrifugation using Lympholyte™ (cat#DVCL5020-10 from Lonza). CD3+ T lymphocytes were isolated via magnetic separation with a Pan T cell Isolation Kit (cat#130-096-535 from Miltenyi Biotech ...
-
bioRxiv - Cell Biology 2022Quote: ... Dissociated muscles were passed through a 70µm filter and red blood cells were lysed in ACK lysis buffer (Lonza #10 548E). Cell suspensions were then incubated with anti CD45 ...
-
bioRxiv - Physiology 2022Quote: ... 1 million cells were washed with PBS and electroporated with 5-10 μg of appropriate plasmid using Amaxa cell nucleofector kit T (Lonza laboratories). Cells were allowed to recover for 48 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... HUVEC cells were maintained in growth factor-supplemented media (EBM + EGM-2 aliquots + 10% FBS) from Lonza (C-3121, CC-4542). MDA-MB-231 cell line was modified to express GFP (green fluorescent protein ...
-
bioRxiv - Genetics 2023Quote: ... The pellet was washed with 10 ml of 1 x PBS and resuspended in 20 µl of nucleofection SF buffer (Lonza Biosciences), in which 600 ng of the DsRed2-N1 plasmid was added to the suspension ...
-
bioRxiv - Immunology 2024Quote: ... and spun at 200xg for 10 min and resuspended at a concentration of 2-10x106 cells/100µL in Lonza Buffer P3/supplement (Lonza #V4XP-3024). The RNP complex and 100µL of resuspended cells were combined and electroporated using pulse code EO-115 on the Lonza 4D-Nucleofector Core/X Unit.
-
bioRxiv - Physiology 2024Quote: The gRNA plasmid (1µg) and donor template plasmid (3µg) were introduced into 3T3-L1 cells (1X 10 6 cell) by electroporation (LONZA, Basel, Switzerland). After 48h of electroporation ...
-
bioRxiv - Bioengineering 2024Quote: ... with L-ascorbic acid-2-phosphate (AA2P) (5mg/ml stock, 10 µl/ml), and β-glycerolphosphate (BGP) (20 mM stock, 20 µl/ml) (Lonza). The cell culture medium was changed every 3 days ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatants were pelleted at 350 xg for 10 min and resuspended in Endothelial Cell Growth Basal Medium-2 (EBM-2, CC-3156, Lonza, Morristown, NJ). Isolated NPCs were stained with SYTOX® Red (5uM ...
-
bioRxiv - Cell Biology 2020Quote: 1 million Jurkat cells were electroporated with the 2.5 μg of sgRNA plasmid and GFP control plasmid at a 10:1 ratio using an Amaxa Cell Line Nucleofector Kit V and an Amaxa™ Nucleofector™ II (Lonza). HeLa ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4×106 cells were electroporated with 10 μg DNA of pBluescript II SK(+) carrying a human U6 promoter and EGFP-sgRNA using Nucleofector® V Kit (Lonza, Switzerland) as described above.
-
bioRxiv - Neuroscience 2019Quote: ... Co-transfection of 500 ng sgRNA plasmid and 1 µl of 10 µM ssODN template into 2×105 HEK293 cells was performed using nucleofection on the 4D-Nucleofector system (LONZA, Basel, Switzerland), as described previously38 ...
-
bioRxiv - Systems Biology 2020Quote: ... The pellets were resuspended in 10 mL of complete mammary epithelial growth medium with 2% v/v FBS without GA-1000 (MEGM; Lonza CC-3150). Cells were incubated at 37 °C for 1 h ...
-
bioRxiv - Systems Biology 2020Quote: Single-cell suspensions were pelleted at 400 x g for 5 min and washed once with 10 mL mammary epithelial basal medium (MEBM; Lonza CC-3151). For each sample ...
-
bioRxiv - Cancer Biology 2021Quote: ... To generate IKZF3-deficient CAR-T cells, 15-20 million PBMCs in RPMI 1640 (10% FBS, 1% penicillin/streptomycin) were nucleofected using Nucleofector 2b (Lonza, Köln, Germany) with a mixture of 4 plasmids (5 μg each ...
-
bioRxiv - Microbiology 2021Quote: ... was combined with 30 pmol total synthetic sgRNA (10 pmol for each sgRNA) (Synthego) to form RNPs in 20 μL total volume with SE Buffer (Lonza #V5SC-1002). The reaction was incubated at room temperature for 10 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... used to verify viral titers were maintained in Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 100 μg/mL penicillin and 100 μg/mL streptomycin (Lonza, Walkersville, MD) at 37°C in an atmosphere of 5% CO2 and ≥95% humidity ...
-
bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with ACK Lysing Buffer (10-548E, Lonza Bioscience, Switzerland) to remove red blood cells ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were electroporated with 10 µg lineage recorder DNA and 1 µg Sleeping Beauty transposase in 100 µl Human Stem Cell nucleofection buffer (Lonza, VVPH-5022) using the B-016 program of the 4D-Nucleofector (Lonza ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and used to infect fresh Sf9 cells (1.2 × 106 cells* ml) in 10 ml of Insect-Xpress medium (Lonza, Walkersville, MD, USA) with 15 mg/ml gentamycin (Roth ...
-
bioRxiv - Microbiology 2022Quote: ... schizonts purified from an overnight culture of PbDiCre parasites were transfected with 5–10 µg of linearised plasmid by electroporation using the AMAXA Nucleofector device (Lonza, program U033), as previously described (Janse et al. ...
-
bioRxiv - Physiology 2020Quote: Growth media used for the expansion of human muscle-derived cell populations consisted of Hams F-10 nutrient mix (Lonza, Basel, Switzerland) with added L-glutamine (2.5 mM) ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Biochrom AG, Berlin, Germany) and 1% (v/v) antibiotic–antimycotic solution (AAS, Lonza, Basel, Switzerland) was used for culturing human dermal fibroblasts (HDF ...
-
bioRxiv - Immunology 2019Quote: ... were sorted into B cell media (IMDM medium, GIBCO; 10% heat-inactivated low IgG FBS, Life Technologies; 5 ml GlutaMAX, Life Technologies; 1 ml MycoZap plus PR, Lonza). Immediately following the sort ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza, Walkersville, MD, USA). The generation number of ECs is maintained between 30-40 generations ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were maintained in RPMI 1640 medium supplemented with 2 mM Glutamine and 10% (v/v) heat-inactivated FBS (Lonza Group Ltd.) containing 100µg streptomycin ml−1 and 100 U penicillin ml−1 and passaged as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μg of pX330 neomycin-resistant vector containing each gRNA and 10 mM ssODN were co-transfected into 2 × 106 ESCs using a Nucleofector (Lonza, program A023). Cells were plated onto two 10 cm dishes containing hygromycin-resistant DR4 MEFs ...
-
bioRxiv - Microbiology 2022Quote: ... pre-coated cell culture 6-well plate with EBM-2 endothelial media supplemented with 10% FBS and a growth factor bullet kit (Lonza, Walkersville, USA) 3 mL/well ...
-
bioRxiv - Genomics 2023Quote: ... was mixed with 16.4 μL Nucleofector SolutionTM and 3.6 μL Supplement and incubated at room temperature for about 10 min according to the instruction of Amaxa 4D-Nucleofector X Kit TM (Lonza, #V4XP-3032). HepG2 ...
-
bioRxiv - Immunology 2023Quote: ... 80 µl of supernatant was removed and replenished with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
-
bioRxiv - Cell Biology 2023Quote: CD34+ cells collected from each animal on the two days of apheresis were placed in culture for 48 hours in X-VIVOTM 10 (Lonza, Walkersville, MD) supplemented with 1% human serum albumin (HSA ...
-
bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
-
bioRxiv - Cell Biology 2024Quote: The NK-92 cell line was acquired from the American Type Culture Collection and cultured in X-VIVO 10 (Lonza, 04-380Q) supplemented with 20% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...
-
bioRxiv - Immunology 2020Quote: ... followed by washing in complete media (RPMI-1640 containing 10% FBS, 2 mM glutamine, 100 IU/ml penicillin, and 100 µg/ml streptomycin, Lonza, Walkersville, MD, USA). Isolated BAL cells (approx.1-2 x106 ...
-
bioRxiv - Microbiology 2022Quote: ... mixed with DNA and/or RNA as indicated in each experiment and electroporated at 110 V for 10 ms by the electroporator (Lonza Amaxa Nucleofector 2B). After electroporation ...
-
bioRxiv - Molecular Biology 2023Quote: ... were grown in 10 cm2 dishes in a humidified environment at 37°C with 5% CO2 in DMEM/F12 medium (Lonza Ltd. Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... were expanded in 10 cm2 dishes in a humidified environment at 37°C with 5% CO2 in DMEM/F12 medium (Lonza Ltd. Basel, Switzerland) supplemented with 12.5% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: PEO1 cells were maintained in RPMI+ 10% heat inactivated FBS (Avantor, Cat. #10803-034) + 100 μg/mL Penicillin/Streptomycin (Lonza, Cat. #09-757F). EHMT1 or EHMT2 overexpression cells were obtained by using lentivirus to infect cells with either TRE_EHMT1_hygromycin or TRE_EHMT2_hygromycin ...
-
bioRxiv - Genomics 2023Quote: ... the cells were pelleted at 100x g for 10 minutes and resuspended in room temperature Lonza P3 buffer (Lonza, catalog no. V4XP-3032) at 1.5x106 cells per 20 ul P3 ...
-
bioRxiv - Genomics 2021Quote: ... were thawed onto a 60 mm tissue-treated culture dish seeded with irradiated mouse embryonic fibroblasts (MEFs) as feeders in DMEM high glucose base medium supplemented with 15% fetal bovine serum (FBS, Lonza cat. no. 14-501F lot no. 0000217266), 1X Pen/Strep ...
-
bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
-
An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsbioRxiv - Neuroscience 2022Quote: ... The pellet was then resuspended in DMEM supplemented with 10% (v/v) foetal bovine serum (FBS) with antibiotic-antimycotic (Lonza #12-707F, ThermoFisher #15240062). The cells were then titrated with fire polished glass pipettes with different apertures ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell line was authenticated through short tandem repeat analysis and tested for mycoplasma contamination every 10 passages using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). PDGFR-BiFC and myr-Venus stable cells were cultured in RPMI growth medium [RPMI 1640 (Gibco ...