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Citations for Lonza :
101 - 150 of 162 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... using the Cell Line Nucelofector Kit V and the Amaxa Nucleofector II Device (Lonza Group AG, Basel, CH). Stable cells were selected for with 1mg/mL hygromycin B (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... T cells were subsequently transfected by electroporation of RNP using setting E0115 of the Nucleofector II device (Lonza). Cells were cultured overnight at density of 3×106 cells/mL in complete RPMI supplemented with 5 ng/mL IL-7 and IL-15 to account for cell loss after electroporation (∼33%) ...
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bioRxiv - Developmental Biology 2020Quote: ... a 1:1 mix containing EGM2-Incomplete (EGM2 media without supplementation with EGF, FGF2 and VEGF-A; Lonza) and macrophage media (v/v ...
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bioRxiv - Genomics 2020Quote: ... Exactly 100μl of the transfection mix in Amaxa cuvette was electroporated in a Nucleofector 2B Device (Lonza™) using the program U33 ...
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bioRxiv - Genetics 2019Quote: ... Hela cells were electroporated with plasmids using Ingenio electroporation kit (SopaChem) and the Nucleofector™ II/2b device (Lonza). Cells were harvested 24h post-electroporation ...
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bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the cell pellet was transfected with 1.0 μg of cheriff eGFP using a Nucleofector IIS (Lonza, Basel, Switzerland) and Amaxa Basic Neuron SCN Nucleofector Kit (VSPI—1003 ...
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bioRxiv - Biochemistry 2021Quote: ... then the ventricles were dissected and dissociated by serial digestion at 37°C with 0.44 mg/ml (6800 U) Worthington Type II collagenase (supplied by Lonza) and 0.6 mg/ml pancreatin (Sigma-Aldrich ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: SiRNAs or overexpressing plasmids were transfected into day 4 differentiated BAT1 brown adipocytes using Amaxa Nucleofector II Electroporator (Lonza) with an Amaxa cell line nucleofector kit L according to the manufacturer’s instructions (Lonza ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: A total of 3-4µg of plasmid DNA was electroporated into 3-4x106 early passage ES cells using the Amaxa Nucleofector II (Lonza) programme A-023 ...
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bioRxiv - Molecular Biology 2019Quote: ... A total of 5 × 105 cells were suspended in 100 μL of Nucleofector V solution mix (Lonza, VCA-1003) with 2 μg of GZMA and RASGRP1 CRISPR/Cas9 plasmids and subjected to nucleofection using the cell line-specific program T-O17 ...
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bioRxiv - Genomics 2019Quote: ... one million cells were mixed with 20 μg plasmids and 100 μL supplemented Nucleofector Solution V and electroporated with a Nucleofector II device (Lonza) using Program T-016 ...
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bioRxiv - Microbiology 2020Quote: ... 3 x 107 bloodstream form cells were harvested by centrifugation and transfected with 5-10 μg of linearized plasmid DNA using an Amaxa Nucleofector II (Lonza) with program X-001 ...
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bioRxiv - Molecular Biology 2019Quote: ... POLE3 and POLE4 gene knockouts were generated in RPE1-hTERT Flag-Cas9 TP53-/- cells by electroporation of LentiGuide-Puro or LentiGuide-NLS-GFP vectors using an Amaxa II Nucleofector (Lonza). C16orf72 gene knockout clones were generated in RPE1-hTERT Flag-Cas9 TP53-/- cells by transfecting sgRNA/Cas9 ribonucleoprotein complex using Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... Electroporation was performed using the Human Adult Dermal Fibroblast Nucleofector™ Kit and the Nucleofector™ II/2b device (Lonza). Both cell lines were harvested 72-hours post-transfection for qRT-PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... TXNDC17 gene knockouts were generated by electroporation of LentiGuide vectors (see Table S6) using an Amaxa II Nucleofector (Lonza, Basel). 24 h after transfection ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was transferred to an electroporation cuvette and electroporated using program X-001 on an Amaxa Nucleofector II (Lonza). Cells in the cuvette were mixed with 300 μl of DMEM ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were dissociated into single cells with accutase and 1 million cells were used for nucleofection with 5 μg of gRNA/Cas9 plasmid and 5 μg of each donor plasmid (WT and G12D) using Nucleofector II (Lonza) and program B-16 ...
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bioRxiv - Microbiology 2021Quote: ... Raw264.7 Ifit2 CRISPR cells were generated by electroporating low passage Raw264.7 cells with Ifit2 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 2 x 106 cells of MEFs were transfected with 1.2 nM of siRNAs using Amaxa Nucleofector II (Lonza, Basel, Switzerland) and MF 1 Nucleofector kit (Lonza ...
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bioRxiv - Developmental Biology 2021Quote: ... were mixed with 2 μg of the indicated constructs (pN2-amphiregulin-myc, pN2-myc-amphiregulin, pN2-Tig1-myc, pN2-myc-Tig1) and nucleofected using a Nucleofector II (Lonza) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Activated cells were transfected using the Human T cell Nucleofector Kit (Amaxa Biosystem, #VPA-1002) and the Amaxa Nucleofector II system (Lonza), Program T-023 with ribonucleoprotein complexes ...
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bioRxiv - Molecular Biology 2022Quote: ... The suspension was transferred to an electroporation cuvette and electroporated using the Q-001 program on an Amaxa Nucleofector II (Lonza). The cells were recovered from the cuvette ...
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bioRxiv - Genomics 2022Quote: ... cells were seeded in a 10 cm dish and transfected with vectors encoding both Cas9 and sgRNA target sequence using an Amaxa Nucleofector II instrument (Lonza). Forty-eight hours after transfection single GFP+ cells were sorted into 96-wells plates on FACS ARIA II/III Flow Cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 107 epimastigotes in 0.5 mL were incubated with 30 μg of the plasmids in the buffer as described [101] and pulsed twice with the AMAXA Nucleofactor II electroporator (Lonza) X-014 program ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were co-transfected with 2 µg of the CRISPR/Cas9 plasmid and 2 µg of ssODN (50 µM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µg of PX459 V2.0 containing the sgRNA sequence were electroporated into 2×106 DVI2 ESCs (HM1 background) using program A023 of the Amaxa Nucleofector II (Lonza) to ablate endogenous Shh gene expression ...
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bioRxiv - Cell Biology 2023Quote: Differentiated HL-60 cells were nucleofected with Amaxa Nucleofector II device and Amaxa Cell line kit V (Lonza, VACA-1003) and prepared for live-cell imaging using aslightly modified version of an existing protocol 96 ...
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bioRxiv - Neuroscience 2023Quote: Transient electroporation: Cultured neurons were electroporated using the Lonza Nucleofector II device (Cat Number: AAD-1001N, Lonza; program O-003) and the mouse neuron Nucleofector® Kit (Cat Number ...
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bioRxiv - Microbiology 2024Quote: Raw264.7 CRISPR cells were generated by electroporation of low passage Raw264.7 cells with Dhx58 and Ifih1 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...
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bioRxiv - Cell Biology 2023Quote: ... 50µg DNA of the resulting constructs were linearized with endonuclease within the gene fragment and transfected using Amaxa Nucleofector II device (Lonza) into RH TIRΔKU80Δhxgprt and RH ΔKU80Δhxgprt parents respectively with 100 µl Cytomix buffer supplemented with 20mM ATP and 50mM reduced glutathione ...
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bioRxiv - Cell Biology 2023Quote: ... 2005) using Amaxa Basic Parasite Nucleofector Solution 1 using the X-001 program of an Amaxa Nucleofector II (Lonza, Switzerland). Clones were selected by growth in medium containing phleomycin (2.5 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: hTERT immortalized RPE-1 (RPE1) cell lines were grown in an 1:1 mix of DMEM and F-10 (Lonza) and Human Embryonic Kidney (HEK ...
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bioRxiv - Cell Biology 2020Quote: ... The cell / Amaxa solution mixture was added to the RNP mix and then pipetted into the bottom of a 96-well nucleofection plate (Lonza). This sample was then nucleofected using the 4D-Nucleofector Core unit and 96-well shuttle device (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... 01F49i-N-B7 iPSCs were dissociated to single cells and 250,000 cells transfected with 25 μL of the prepared transfection mix containing 20 µL of nucleofection buffer (P3 Primary Cell 4D-NucleofectorTM X Kit S, Lonza), 5 µL of the RNP complex ...
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bioRxiv - Genomics 2023Quote: ... 5 µg of the 4sgRNA plasmid or 10 µM synthetic guide RNAs were mixed and the cell/reagent/nucleofection mix was transferred to Nucleofection cuvette strips (Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Developmental Biology 2020Quote: ... and nucleofected with BioID constructs using an Amaxa Nucleofector II and the Mouse Neural Stem Cell Nucleofector Kit (Lonza; VPG-1004) according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was transiently transfected into HEK SNAP-β2 β-arr-KO cells by electroporation with the Amaxa Nucleofector II device (Lonza). The included program Q-001 is designed for high expression levels in HEK cells and was used according to the manufacture’s protocol ...
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bioRxiv - Biophysics 2019Quote: The HEK293 adherent culture cells were transfected with pcDNA3.4-PCV2d plasmid using Amaxa Nucleofector II instrument by Lonza (program A-23). The transfected cell were plated on 6-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... MDCKII cells were transfected by electroporation with plasmids encoding CD4-SIV Env chimeras using an Amaxa Cell Line Nucleofector™ Kit L and Amaxa Nucleofector II with settings L-05 (Lonza). Stable transfectants were selected with 400 μg/ml G418 Sulphate (Calbiochem) ...
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bioRxiv - Immunology 2020Quote: ... Six micrograms of plasmid DNA encoding the cDNA of the desired proteins was used for a single transfection reaction using program T-019 on the Amaxa Nucleofector II (Lonza, MD).
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bioRxiv - Cell Biology 2023Quote: ... brucei 13-90 cells were electroporated with 10 µg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (program X-001) (Lonza, Switzerland).
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bioRxiv - Cancer Biology 2022Quote: ... and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza, V4XP3032). Cells were electroporated with the program EO-100 using the Lonza Nucleofector ...
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bioRxiv - Cancer Biology 2022Quote: ... Pelleted organoids were resuspended in 20uL of nucleofection mix and transferred to electroporation chamber (Lonza kit, #V4XP-3032, 96-well format) for electroporation using Lonza X Unit Nucleofector under the [ES ...
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bioRxiv - Biophysics 2021Quote: ... for all imaging experiments involving exogenous expression we used the Lonza Amaxa II Nucleofector System with Cell Line Nucleofector Kit V reagent (Lonza VCA-1003). Briefly ...
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bioRxiv - Cell Biology 2020Quote: 1 million Jurkat cells were electroporated with the 2.5 μg of sgRNA plasmid and GFP control plasmid at a 10:1 ratio using an Amaxa Cell Line Nucleofector Kit V and an Amaxa™ Nucleofector™ II (Lonza). HeLa ...
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bioRxiv - Developmental Biology 2021Quote: Human isogenic ESCs containing different lengths of CAG repeats in the HTT Exon1 locus (HD-RUES2 6) were nucleofected using the Cell Line Nucelofector II (Kit L from Lonza, Walkersville, MD) by applying the B-016 program ...
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bioRxiv - Molecular Biology 2020Quote: ... 4×106 cells were electroporated with 10 μg DNA of pBluescript II SK(+) carrying a human U6 promoter and EGFP-sgRNA using Nucleofector® V Kit (Lonza, Switzerland) as described above.
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...