Labshake search
Citations for Lonza :
51 - 100 of 162 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Plasmids were introduced into neurons using Nucleofector II (Lonza, Basel, Switzerland) immediately before seeding or using Lipofectamine 2000 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2019Quote: ... 5mM EDTA and 1% antibiotic mix (pen-strep-AmphoB; Fisher-Lonza). After incubation ...
-
bioRxiv - Biochemistry 2019Quote: ... and electroporated using program U-013 on a Nucleofector II device (Lonza). Following electroporation ...
-
bioRxiv - Synthetic Biology 2019Quote: ... PC12 cells were transfected by electroporation using the Amaxa Nucleofector II (Lonza). Cells were added to a suspension of DNA in electroporation buffer (7 mM ATP ...
-
bioRxiv - Biochemistry 2020Quote: ... and electroporated using program U-013 on a Nucleofector II device (Lonza). Following electroporation ...
-
bioRxiv - Neuroscience 2020Quote: Transfection in primary cortical neurons was performed using Amaxa Nucleofector II (Lonza) with the Ingenio electroporation kit (Mirus Bio) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Enhancer vectors were transfected using the Amaxa™ Nucleofector™ II (Lonza) with Cell Line NucleofectorTM Kit V (Lonza ...
-
bioRxiv - Synthetic Biology 2019Quote: PC12 cells were transfected by electroporation using the Amaxa Nucleofector II (Lonza). Cells were added to a suspension of DNA in electroporation buffer (7 mM ATP ...
-
bioRxiv - Synthetic Biology 2020Quote: ... U2OS cells were transfected via electroporation using the Amaxa Nucleofector II (Lonza). Cells were added to a suspension of DNA in electroporation buffer (7 mM ATP ...
-
bioRxiv - Cell Biology 2023Quote: ... LUHMES cells were transfected by Nucleofection in a Nucleofector II device (Lonza) using a Basic Nucleofector Kit for primary neurons (Lonza ...
-
bioRxiv - Microbiology 2019Quote: ... 4ml of overlay containing a 1:1 ratio of 2x Eagle’s Minimal Essential Medium (EMEM) with 1x Pen/Strep/Glutamine (Lonza, Mount Waverley, VIC, Australia) and 1.5% agarose (Promega ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.5 × 106 cells were electroporated on a Nucleofector II device (program A032, Lonza) with 36 pmol SpCas9 Nuclease V3 ...
-
bioRxiv - Microbiology 2020Quote: ... siRNA was transfected with Amaxa cell line nucleofector kit and Nucleofector II (Lonza) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2022Quote: Nucleofection of hCEC cells was carried out using the Amaxa Nucleofector II (Lonza), optimized for the HCT116 cell line ...
-
bioRxiv - Developmental Biology 2020Quote: ... ESCs were electroporated using Nucleofector II (Amaxa) and mouse ESC Nucleofector kit (Lonza). Afterwards ...
-
bioRxiv - Developmental Biology 2021Quote: ... Nucleofection was performed with program B-016 on an Amaxa Nucleofector II (Lonza). Cells were then grown for ten days under selection by 1 μg/mL puromycin and 10 μg/mL blasticidin ...
-
bioRxiv - Molecular Biology 2021Quote: ... The RAP80-/- knockout cell lines were generated by electroporation (Lonza Amaxa II Nucleofector) of plasmids expressing sgRNAs (5′- ATTGTGATATCCGATAGTGAT-3′ and 5′-GTTCTGTCAGTGTGAAGAGG-3′ ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK STIM1/2-/- were transfected via electroporation using the Amaxa Nucleofector II (Lonza) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... CREBBPR1446C gene-editing reagents were introduced by nucleofection (Nucleofector II, Kit R, Lonza) consisting of a gRNA/Cas9 RNP complex (IDT 1072532 ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were electroporated using program X-001 on the Amaxa Nucleofector II (Lonza). Transfected cells were cultured in DMEM growth media without any antibiotics for 24-48 hrs and then selected for 10 days with 1ug/ml puromycin (Thermo Fisher #A1113803 ...
-
bioRxiv - Cancer Biology 2023Quote: ... a plasmid mix was prepared consisting of 1uL of Electroporation Enhancer (100uM, Lonza), 1.5 uL of 2ug/uL ABE8e-NRCH ribonucleoprotein editor,83 1uL of base editor primer (50uM stock ...
-
bioRxiv - Immunology 2022Quote: ... bergei were transfected with the target plasmids using Amaxa parasite nucleofection kit II (Lonza), by employing the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... The nucleofection was performed using an Amaxa Nucleofector II (Lonza Bioscience, program B-016). After selection with 1 μg/ml puromycin ...
-
bioRxiv - Immunology 2023Quote: ... 5x106 T- cell were electroporated (Amaxa Nucleofector II) in 100 µl electroporation buffer (Lonza) in the presence of Cas9-RNP and 2 µg of DNA-template ...
-
bioRxiv - Cancer Biology 2023Quote: ... HPAF-II cells were assessed for mycoplasma using the MycoAlert PLUS Detection Kit (Lonza). Cells were cultured in a Thermo Scientific Forma Steri-Cycle i160 incubator at 5% CO2 and 37°C for normal culture ...
-
bioRxiv - Molecular Biology 2023Quote: ... We then performed electroporation using the Amaxa® Nucleofector® II Device from Lonza with the mouse ESC (A-013 ...
-
bioRxiv - Bioengineering 2022Quote: ... 7.1 μL of the reaction mix were placed on a Gel Slick™ (Lonza)-coated glass slide ...
-
bioRxiv - Cell Biology 2021Quote: Transient transfection was performed by electroporation using a Nucleofector II instrument (Lonza, Wakersville, MD, USA) and Lonza nucleofection kit V (Lonza ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 50 mM mannitol)23 and program V-024 of an Amaxa Nucleofector II (Lonza). T cells were allowed to recover in complete RPMI-1640-sodium bicarbonate medium and transfection efficiency was evaluated after 24h by flowcytometry and/or western blotting ...
-
bioRxiv - Cell Biology 2023Quote: Human ESCs were nucleofected using an Amaxa nucleofector II in Nucleofector Solution L (all Lonza) using B-016 preset ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were electroporated in 0.2-cm gap electroporation cuvettes by Amaxa Nucleofector II (Lonza) using protocols pre-installed by the manufacturer.
-
bioRxiv - Developmental Biology 2021Quote: ... Samples on day 6 were dissociated with enzyme mix composed of 0.5×Versene (Lonza, 17711E), 0.5×Accumax (STEMCELL Technologies ...
-
bioRxiv - Bioengineering 2022Quote: ... The AAV mix was added diluted in hepatocyte basal media (HBM, Lonza, Cat# CC-3199). Media was changed daily for three days ...
-
bioRxiv - Cancer Biology 2022Quote: ... nucleofection mix was prepared as follows: 16.4 uL Primary P3 Buffer (Lonza kit, #V4XP-3032), 3.6 Supplement 1 (Lonza kit ...
-
bioRxiv - Cell Biology 2023Quote: ... Electroporation mix was prepared resuspending RPE1 cells in P3 Primary Cell Full Electroporation Buffer (Lonza), and adding previously prepared RNPs ...
-
bioRxiv - Cell Biology 2020Quote: ... and 7 mM Na2HPO4 in nuclease-free water) using either the Amaxa nucleofector system II (Lonza) under nucleofection program T-005 or the Amaxa 4Dnucleofector system (Lonza ...
-
bioRxiv - Neuroscience 2020Quote: ... RNP complexes were transfected in 500,000 cells with Amaxa Nucleofector II (Amaxa Biosystems, Lonza, Basel Switzerland) using the P-022 program according to manufacturer’s protocol and adding 1 ul of 100 μM Alt-R® Cas9 Electroporation Enhancer (IDT ...
-
bioRxiv - Cancer Biology 2022Quote: ... 4 μg guide RNA and 200 pmol ssODN using the Amaxa Nucleofector II (Lonza, Basel, Switzerland). Cells were recovered in growth medium for 24 hr and then sorted as single cells into 96-well plates by FACS ...
-
bioRxiv - Molecular Biology 2020Quote: ... For transfections HEK cells were transfected via electroporation unsing the Amaxa® Nucleofector II® (Lonza) according to the user manual ...
-
bioRxiv - Bioengineering 2021Quote: ... ii Quantification of monolayer hydraulic conductivity for two cell lines (Lonza, light blue; ScienCell, dark blue) and for two dextran molecular weights (MW) ...
-
bioRxiv - Cancer Biology 2022Quote: ... ii) Adherent cell monolayers at an 80% confluence were dissociated with different concentrations trypsin-EDTA (LONZA), 10X (0,25% v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... was achieved using a Nucleofector II electroporator and the Cell Line Nucleofector Kit V (Lonza Biosciences). MEFs and MDA-MB-231 cells were stably modified using BFP and DN-KASH expression plasmids in a doxycycline-inducible Piggybac plasmid backbone (Addgene #187019) ...
-
bioRxiv - Neuroscience 2020Quote: ... Each mix was transferred to a Nucleocuvette and electroporated using the 4D-nucleofector Core Unit (LONZA) using the program CA-137 ...
-
bioRxiv - Biochemistry 2020Quote: ... 50 mM HEPES pH 7.3) using the X-001 program of an Amaxa Nucleofector II (Lonza, Switzerland) (Burkard et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... using the Human CD34+ nucleofector kit and the Nucleofector II device (both from Lonza Group Ltd., CH) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... pX459-derived expression plasmids were transfected into MDCK cells by an Amaxa Nucleofector II (Lonza, Basel, Switzerland). The transfected cells were selected with 2.0 µg mL-1 puromycin ...
-
bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s instructions and the T-018 program of the Amaxa Nucleofector II device (Lonza). After nucleofection ...
-
bioRxiv - Cell Biology 2023Quote: Stable RPE1 cells (∼0.5×106) were electroporated with pmScarlet_peroxisome_C1 (or mScarlet-PTS1) (250 ng) using an Amaxa Nucleofector II (Lonza) in accordance with the manufacturer’s instructions using Ingenio electroporation solution (Mirus) ...
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant plasmids were verified by Sanger sequencing and electroporated into E14 cells using the Nucleofector II platform (Lonza) (program A-013) ...
-
bioRxiv - Microbiology 2020Quote: ... Nucleofection was performed using the program X-001 for Jurkat T-cells (Amaxa™ Nucleofector™ II, Lonza). Cells were cultured in RPMI-1640 medium media supplemented with 10% fetal bovine serum (FBS ...