Labshake search
Citations for Lonza :
51 - 100 of 190 citations for Dengue Virus serotype 4 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Residual endotoxins in the purified phage preparations were determined by the Limulus Amebocyte Lysate (LAL) Kinetic-QCL Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was determined to be more than 99% by sodium dodecyl sulfate and polyacrylamide gel (SDS-PAGE) with an endotoxin concentration lower than 2 units/mg protein measured by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza). In previous experiments we demonstrated that these recombinant proteins were functionally devoid of significant endotoxin contamination ...
-
bioRxiv - Immunology 2019Quote: ... Mucin proteins was prepared as previously described45 and confirmed to be endotoxin free using a Limulus Amebocyte Lysate (LAL) assay kit (Lonza, USA). Full details of mucin protein purification and preparation are shown in supplementary methods ...
-
bioRxiv - Immunology 2023Quote: ... acetylated chitin oligomers were suspended in endotoxin free water and tested for endotoxin level by using the limulus amebocyte lysate (LAL) assay (Lonza, CH). Levels below 0.25 EU/ml (<25 pg/ml LPS ...
-
bioRxiv - Cell Biology 2020Quote: Electroporation was performed using an Amaxa 4-D device (Lonza) or a Neon Transfection System (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 days after nucleofection with the AMAXA Nucleofector Kit (Lonza), we applied puromycin selection until we observed the appearance of green colonies ...
-
bioRxiv - Developmental Biology 2020Quote: ... embedded in 4% low melting temperature agarose (Lonza, Cat # 50100) and sectioned in a vibratome to produce 300μm-thick coronal slices ...
-
bioRxiv - Microbiology 2019Quote: ... a solution of 4% (wt/vol) SeaPlaque GTG agarose (Lonza) in MilliQ water was sterilized for 30 minutes at 250°C ...
-
bioRxiv - Bioengineering 2023Quote: ... Normal human dermal fibroblasts (NHDFs; Lonza; passages 4 to 10) were cultured on dishes in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Neuroscience 2024Quote: ... and mounted in 4% low melting point agarose (50100, Lonza) for sectioning (70 μm ...
-
bioRxiv - Immunology 2023Quote: ... The levels of endotoxin in the purified protein samples were assessed using the QCL-1000 Limulus amebocyte lysate system (Lonza, Basel, Switzerland), and it was found that the recombinant proteins had endotoxin levels of ∼ 4 pg/µg ...
-
bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
-
bioRxiv - Cell Biology 2021Quote: ... endotoxin-free phosphate buffered saline (PBS) and verified to be free of detectable lipopolysaccharide contamination by Limulus lysate assay (Lonza, Walkersville, Maryland, USA), MCSF (10 ng/mL ...
-
bioRxiv - Neuroscience 2019Quote: ... 4 mM L-glutamine and 600 U/ml penicillin-streptomycin (Lonza). Reporter-cells (HEK293 stably expressing TetON-NbSyn87-EGFP-P2A-mCherry ...
-
bioRxiv - Neuroscience 2022Quote: ... pCE-hOCT3/4 and pCE-mp53DD using the Amaxa Nucleofactor (Lonza). The cells were then cultured in TeSR-E7 medium for 3-4 weeks on Matrigel-coated dishes (Corning ...
-
bioRxiv - Biophysics 2021Quote: ... in passages 4-8 were cultured in EGM2-MV medium (Lonza) at 37°C in a humidified atmosphere of 95% air and 5% CO2 ...
-
bioRxiv - Cell Biology 2022Quote: ... in passages 4-8 were cultured in EGM2-MV medium (Lonza) at 37°C in a humidified atmosphere of 95% air and 5% CO2 ...
-
bioRxiv - Microbiology 2022Quote: ... Digested products were then visualised on a 4% NuSieve (Lonza, 50090) agarose gel with SafeView Nucleic Acid Stain.
-
bioRxiv - Immunology 2023Quote: ... embedded in 4% low melting point agarose (GTG-NuSieve Agarose, Lonza) and sectioned into 500 μM slices using a vibratome (VT1000S ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... non-CF#4 (CC-2540S -20TL356517, Lonza, female, 48 years-old). hAEC cells were cultured for several days until reaching 90-95% confluency in complete PneumaCultExPlus medium ...
-
bioRxiv - Cell Biology 2023Quote: ... 1uL electroporation enhancer and 4 uL PBS (Lonza Biosciences, Lexington, MA). Each cell suspension was added to individual wells of an electroporation cuvette (Lonza Biosciences ...
-
bioRxiv - Developmental Biology 2024Quote: ... embedded embryos in 4% NuSieve GTG agarose (Lonza, Rockland, ME, USA), and sectioned at 50 µm (acvrl1 ...
-
bioRxiv - Bioengineering 2023Quote: ... The brain was then embedded in 4% agarose (SeaPlaque agarose, Lonza) hydrogel ...
-
bioRxiv - Cell Biology 2024Quote: ... in passages 4-8 were cultured in EGM2-MV medium (Lonza).
-
bioRxiv - Molecular Biology 2020Quote: ... and 24 h later transfected with 4 μg of pMaxGFP plasmid (Lonza) using Fugene HD transfection reagent (Promega ...
-
bioRxiv - Systems Biology 2021Quote: ... and routinely passaged every 4∼6 days with Versene (EDTA) solution (Lonza). Selected iPSC clones were further validated for expression of pluripotency markers with immunostaining (Supplementary Fig ...
-
bioRxiv - Systems Biology 2021Quote: ... and routinely passaged every 4~6 days with Versene (EDTA) solution (Lonza). Selected iPSC clones were further validated for expression of pluripotency markers with immunostaining (Chen et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was embedded in 4% low-melting agarose in PBS (SeaPlaque, Lonza), mounted on a vibratome with cold PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by 3-4 days of culture in SmGM complete media (Lonza) supplemented with 1% penicillin-streptomycin to expand cell numbers ...
-
bioRxiv - Neuroscience 2019Quote: ... the brain was embedded in 4% agar (Sea Plaque, Lonza, Cat# 50101) and then sliced in PBS using a vibratome (VT1000S ...
-
bioRxiv - Physiology 2021Quote: ... PCR products were run on 4% Nusieve agarose gel (Cat #50090, LONZA) for higher resolution of bands separation results (Witt ...
-
bioRxiv - Cancer Biology 2023Quote: ... Every 3-4 weeks the medium was additionally supplemented with MycoZap (Lonza) to prevent mycoplasma contamination ...
-
bioRxiv - Neuroscience 2023Quote: ... split into 3-4 wells each and cultured in DMEM-F12 (Lonza) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2023Quote: Primary human umbilical vein endothelial cells (HUVECs; Lonza; passages 4 to 7) were cultured on dishes in Endothelial Cell Growth Medium-2 (EGM-2 ...
-
bioRxiv - Microbiology 2024Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) 25 mM (Lonza, 17-737F), gentamicin 25 μg/ml (Lonza ...
-
bioRxiv - Neuroscience 2024Quote: ... Adult brains were embedded in 4% low melting point agarose (50100, Lonza) and sectioned (70 μm ...
-
bioRxiv - Neuroscience 2021Quote: ... Fixed brains were embedded in 4% low melting agarose (SeaPlaque® Agarose, Lonza) in 1X PBS and sectioned at 50 μm (for juvenile brains ...
-
bioRxiv - Immunology 2019Quote: ... embedded in 4% agarose with a low melting point (GTG-NuSieve Agarose, Lonza), and sectioned into slices of 200-400μm using a vibratome (1000 Plus sectioning system ...
-
bioRxiv - Developmental Biology 2021Quote: ... embedded in 4% agarose with a low melting point (GTG-NuSieve Agarose, Lonza), and sectioned into slices of 400-500μm using a vibratome (VT1000S ...
-
bioRxiv - Microbiology 2022Quote: ... The colony was then overlaid with 4% (w/v) agarose (Lonza; Cat: 50181) and frozen at −80 °C for 15 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... separated on a 2% or 4% agarose gel (Seakem LE agarose, Lonza #50004) by electrophoresis (120 V ...
-
bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged every 3-4 days with Accutase (Lonza, Basel, Switzerland) and seeded on new plates with density 1:5 – 1:10 in iPS medium with ROCK inhibitor Y-27632 (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2023Quote: ... HPAECs were cultured for 4 hours in endothelial basal medium (EBM-2, Lonza) with 2% FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were nucleofected with the 4-D Nucleofector System (Lonza, Cat#AAF-1003X) using the CA-137 program ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2-4 x 105 cells were resuspended in 100uL of P2 medium (Lonza) containing 10 µg of Cas9 or Cas9/guide plasmidic DNA and transferred to a cuvette (Lonza) ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell/DNA mixture was then nucleofected using the 4-D nucleofector system (Lonza) code CB-150 and densely replated on Matrigel coated 6-well plates at 2.5×106 cells per well ...
-
bioRxiv - Immunology 2020Quote: ... Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza) containing 10% FBS (Dominique Dutscher) ...
-
bioRxiv - Microbiology 2020Quote: ... and a specific 131 bp final product isolated from a 4% (wt:wt) MetaPhore (Lonza) DNA gel ...
-
bioRxiv - Immunology 2022Quote: ... for 4 days with complete EGM-2 media (Lonza CC-4147, Lonza CC-3156). Upon reaching 80-90% confluence ...
-
bioRxiv - Immunology 2022Quote: ... for 4 days with complete EGM-2 media (Lonza CC-4147, Lonza CC-3156). Upon reaching 80-90% confluence ...