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Citations for Lonza :
1 - 50 of 190 citations for Dengue Virus serotype 4 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The virus pellet was resuspended to EBM2 (Lonza) media ...
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bioRxiv - Microbiology 2022Quote: ... Virus was removed and 2 mL of overlay media (EMEM 1X Lonza 12-684F ...
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bioRxiv - Immunology 2022Quote: ... Lysate were pellet (500 g 8min) and red blood cells were lysate with 500 µl of ACK lysing buffer (Lonza) for 5min ...
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bioRxiv - Molecular Biology 2020Quote: ... Limulus Amebocyte Lysate Assay (Lonza, Walkersville, MD, USA), 10% neutral buffered formalin (CellPath Ltd ...
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bioRxiv - Microbiology 2022Quote: ... All cells and virus stocks tested negative for mycoplasma using MycoAlert Plus reagents (Lonza). Heat-inactivated serum samples were serially diluted in 3-fold steps in DMEM supplemented with 2% heat-inactivated fetal bovine serum (HI-FBS) ...
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bioRxiv - Biochemistry 2023Quote: ... Virus was concentrated using ultracentrifugation (95000 g for 3 hr) and suspended using OPTI-MEM (Lonza). Viral transfections assays were performed by plating target 293T (ACE2+ve ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were loaded on 12% Prosieve 50 gels (Lonza) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were loaded on 10% ProSieve gel (LONZA, LON50618), then subjected to electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: K562 cells stably expressing proinsulin [12] and HEK293T cells for virus production were cultured in IMDM (Lonza). All media were supplemented with 10% FCS ...
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bioRxiv - Microbiology 2020Quote: ... LPS level was determined using QCL-1000 Limulus amebocyte lysate system (Lonza) and found to be >5 pg/ μg of rfhSP-D.
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bioRxiv - Microbiology 2020Quote: ... All swabs were stored at −80°C in virus transport medium consisting of Minimum Essential Medium (MEM) – Eagle with Hank’s BSS and 25 mM Hepes (Lonza), glycerol 99% (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... The virus growth media was either Minimum Essential Medium – Eagle with Earle’s BSS (MEM Eagle EBSS) from Lonza (Rochester, NY) or Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... HUVECs (Lonza, Passage <4) were cultured in an endothelial growth medium (C-22111 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were co-incubated with virus and then supplemented with overlay medium that contains 1% SeaPlaque agarose (Lonza Bioscience, Basel, Switzerland). After 2 days of incubation ...
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bioRxiv - Immunology 2020Quote: ... the endotoxin levels were measured via the QCL-1000 Limulus amoebocyte lysate system (Lonza) and found to be <5 pg/μg of rfhSP-D ...
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bioRxiv - Immunology 2021Quote: Endotoxin level in the AF was determined by Limulus amebocyte lysate assay (LAL; Lonza) according to the test procedure recommended by the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... T47D cells were transiently transfected with the avian tumor virus receptor A (TVA) by using electroporation (preset “FF150” program, 4D-Nucleofector transfection system; Lonza, Cologne, Germany). The cell suspension was cultured with full medium at 37 °C and 5% CO2 for 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ... For transfection 4 × 106 cells/mL cells were resuspended in ProCHO-4 Medium (Lonza) supplemented with 4 mM Ultraglutamine (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... donors (passage 4-9; Lonza) were grown in complete Endopan-3 medium kit (PAN-Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... or 4-D nucleofector (LONZA) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Endotoxin levels were evaluated by the Limulus amebocyte lysate assay (Endotoxin Testing Services, Lonza Europe), and the amount expressed was negligible ...
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bioRxiv - Microbiology 2021Quote: ... The levels of endotoxin were measured using the Limulus Amebocyte Lysate (LAL) Kinetic-QCL kit (Lonza). Phage or AAVP preparations containing endotoxin levels < 0.05 EU/mL were used in this study.
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 4 mM Ultraglutamine (Lonza). 0.625 μg of plasmid DNAs per million cells followed by 2.5 μg polyethyleneimine per million cells (Polysciences ...
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bioRxiv - Cancer Biology 2022Quote: ... NHEM-Ad cells were grown in MGM-4 Melanocyte Growth Medium-4 BulletKit (Lonza, Cat# CC-3249). We added penicillin (50 U/mL ...
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bioRxiv - Genetics 2019Quote: ... Between 1×10^4 – 5×10^4 cells per condition were resuspended in 20μl of Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the Cas9 gRNA RNP complex ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... supplemented with Ultraglutamine-1 (4 mM; Lonza) and antibiotic-antimycotic (1% ...
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bioRxiv - Cancer Biology 2019Quote: ... were transfected using 4-D electroporator (LONZA), following manufacturer instruction ...
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bioRxiv - Immunology 2022Quote: ... and 4 mM of L-glutamine (Lonza). HLA-E expressing K562 cells were sorted and maintained in complete RPMI supplemented with 2 µg/mL blasticidin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Endotoxin was measured with the Limulus Amoebocyte Lysate (LAL) chromogenic end-point assay (Lonza®, Allendale, NJ, USA).
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bioRxiv - Cell Biology 2023Quote: ... 4 μM Alt-R Cas9 Electroporation enhancer (IDT) and 4 μM single-strand DNA homology template (Ultramer DNA Oligonucleotide, Lonza). After electroporation ...
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bioRxiv - Immunology 2019Quote: ... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
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bioRxiv - Immunology 2021Quote: ... and the levels of endotoxins were measured using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). Fh12 was considered endotoxin free when endotoxin levels gave similar to background levels (<0.1 EU/ml) ...
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bioRxiv - Microbiology 2023Quote: ... and the absence of endotoxin was confirmed using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). nFhGST was concentrated by AMICON Ultra-15 Centrifugal filter unit and adjusted to 1 mg/ml as determined using the Pierce protein bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2021Quote: ... a 4% agarose (SeaPlaqueTM GTGTM Agarose, 50111; Lonza) solution was prepared in 0.1M PBS and left at 45°C in a dry bath ...
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bioRxiv - Biochemistry 2020Quote: One straw of passage 4 HUVECs (Lonza, USA) was thawed in a 35mm dish and cultured in EGM-2 medium until almost 80% confluence is reached ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 4 mmol/L L-glutamine (Lonza), 100 IU/ml Penicillin/100 mg/ml Streptomycin (Lonza) ...
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bioRxiv - Cancer Biology 2019Quote: ... or empty vector using 4-D electroporator (LONZA), following manufacturer instruction ...
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bioRxiv - Neuroscience 2021Quote: ... containing 4% low melting point agarose (50111, Lonza) which was then allowed to solidify at 4°C for a few minutes ...
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bioRxiv - Bioengineering 2020Quote: Passage 4 human mesenchymal stem cells (Lonza, Switzerland) were expanded to passage 5 in an incubator set at 37°C and 5% CO2 and fed with low glucose DMEM and glutamine ...
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bioRxiv - Cancer Biology 2020Quote: ... and embedded in 4% low-melting agarose (Lonza) preheated at 42 °C ...
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bioRxiv - Genomics 2022Quote: ... 4 million cells were electroporated (Amaxa Nucleofection, Lonza) with 25μg of L1-Cag/FLAG_NLS_ORF-1L plasmid and 15μg of pIC-Cre ...
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bioRxiv - Microbiology 2023Quote: ... 4 x 104 in 0.4 mL MEM (Lonza), 10% FBS (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... or Melanocyte Growth Basal Medium-4 (Lonza, Switzerland) after pigmented cells were dominant.
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bioRxiv - Cancer Biology 2020Quote: ... CHO-S cells were resuspended at a density of 4 mio cells/mL in ProCHO 4 medium (Lonza, #LZ-BE12-029Q) supplemented with 8 mM ultraglutamine (Lonza ...
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bioRxiv - Genetics 2019Quote: ... Lysates were resolved by electrophoresis (Mini-PROTEAN TGX Precast Gels, BioRad; or PAGEr EX Gels, Lonza; or home-made gels), proteins transferred onto PVDF membranes (Immobilon P ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin concentrations ranged from 150-175 EU/mL as determined using the limulus amebocyte lysate assay (Lonza, Walkersville, MD, USA). This concentration of ODE has been previously shown to produce optimal experimental effects and is well-tolerated in mice (11 ...
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bioRxiv - Bioengineering 2019Quote: ... topped in 4 mL of EGM-2MV media (Lonza), and incubated at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... M206 and M443 were cultured in (MGM-4, Lonza) supplemented with 1 ml CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: Microspheres were tested for LPS contamination using the Limulus Amebocyte Lysate (LAL) QCL-1000™ kit (Lonza Walkersville, Inc., Olten, Switzerland) according to the manufacturer’s instructions ...