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Citations for Lonza :
1 - 50 of 2158 citations for 20 Hydroxyecdysone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... serum-starved RPE1 cells where trypsinized and 0.5×106 of the cells were resuspended in 20 μl of the P3 Primary Cell 4D-Nucleofector Kit buffer (Lonza). The cell suspension was then nucleoporated with reporter plasmids with DNA lesions and undamaged control plasmids ...
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bioRxiv - Bioengineering 2020Quote: ... with a 20 μl P3 solution kit (Lonza, #V4XP-3032) with 600k PGP1 WT cells in each 20 μl reaction well as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 20 μL of T cells were resuspended in P3 buffer at 5 × 107 cells/mL (Lonza) and added to the electroporation mixture ...
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bioRxiv - Bioengineering 2022Quote: ... 5% CO2 in EGM-2 Bullet Kit (Lonza CC-3162) medium and used between passages 4-8.
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bioRxiv - Genomics 2023Quote: ... into 96-well plates containing 5 μl modified freeze buffer (0.1% NP-40, 7.5. % DMSO, 42.5 % 2X Profreeze-CDM (Lonza) in PBS ...
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bioRxiv - Genomics 2020Quote: ... and 5×104 cells were resuspended in 20 μL SF electroporation buffer prepared with SF supplement (Lonza, Basel, Switzerland). 3 μL RNP complex solution was mixed with the cells and the cells were nucleofected using program DJ-110 on a 4D-Nucleofector (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... RNP were formed by mixing 5 to 9 μgr base editor protein with 1.5 μgr of sgRNA in 20 μL of P3 buffer (Lonza, Amaxa P3 Primary Cell 4D-Nucleofector Kit ...
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bioRxiv - Immunology 2020Quote: ... All isolated Tregs were activated by plate bound anti-CD3 and anti-CD28 antibodies and cultured with X-VIVO 20 media (LONZA #04-448Q) supplemented by 1X Pen/Strep ...
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bioRxiv - Cell Biology 2024Quote: ... EBs are transferred to a 6-well plate with 20 EBs/well and placed in macrophage precursor medium (X-vivo15 (Lonza, BE02-060Q), 100 ng/mL M-CSF (Peprotech ...
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bioRxiv - Systems Biology 2020Quote: ... 24 hours activated DC and T cells were incubated in 96 well plates at a DC/T ratio 1:5 in Xvivo15 medium (Lonza). After 6 days ...
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bioRxiv - Developmental Biology 2022Quote: Primary HDLECs were isolated from neonatal human foreskins as described previously (74) and cultured from passages 5 – 7 on fibronectin-coated plates with EGM-2 MV growth media (Lonza) supplemented with human VEGF-C (R&D) ...
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bioRxiv - Neuroscience 2019Quote: ... of viral supernatants were used to infect E2T cells (5 x 105 cells/well in 6-well plate) for plaque assays and cells were maintained in culture medium containing 5% SeaPlaque agarose (Lonza) until plaques become visible at ∼12 days after infection ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s protocol using the program T-20 and the Amaxa kit R (Lonza). Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 × 103 cells were plated in low-attachment 96-well plates containing 100 μL MEGM media (without BPE) (Lonza, CC-3150), 20 ng/mL bFGF (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 20% FCS (Lonza), 2 mM L-Glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... and re-suspended in 20 μL NF-solution (P3 Primary Cell 4D-Nucleofector™ X Kit, Lonza). Thereafter ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were resuspended in 20 uL of the Amaxa NHDF Nucleofector Kit (Lonza, Walkersville, MD), containing 1ug of each of the gRNA and donor plasmid vectors (ABM ...
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bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary uveal melanoma cells were equally divided onto two wells of a fibronectin-covered 6-well tissue culture plate and grown in 5% CO2 in MDMF medium which consists of HAM’s F12 (Lonza, Walkersville MD, USA) supplemented with 1 mg/mL BSA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... HEPES (20 mM, Lonza), sodium pyruvate (1 mM ...
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bioRxiv - Bioengineering 2023Quote: ... in 6-well tissue culture plates and were allowed to adhere for 5 hours before induction of serum starvation in EBM-2 (LONZA, Cat# CC-3156) for 16 hours prior to being administered media of interest ...
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bioRxiv - Neuroscience 2021Quote: ... coated coverslips in a 24-well plate in 1ml Endothelial Growth Media-2 Bullet Kit (EGM-2; Lonza; Endothelial basal media-2 with 2% Fetal Bovine Serum (FBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cellular ATP levels were detected 20 h after glutamate exposure using the ViaLight™ Plus-Kit (Lonza, Verviers, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... In vitro activated or naïve P14 cells were resuspended in 20 μl of P3 (P3 Primary Cell 4D-Nucleofector X Kit; Lonza), mixed with sgRNA/Cas9 RNP and electroporated using a Lonza 4D-Nucleofector system (CM137 ...
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bioRxiv - Genetics 2020Quote: ... 20 µg ssODNs and 4 µg pSpCas9(BB)-2A-GFP construct using Human Stem Cell Nucleofector Kit 2 (Lonza). For the generation of UE-RASGEF1A-int1-KO and PIK3C2B-int10-KO hPSC lines ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 20% FBS (Lonza) and 1% penicillin–streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... 10,000,000 lymphoblastoid cells were nucleofected with 60 µg of RTW3027 and 20 µg of plasmid expressing gRNA and mCherry using Cell Line NucleofectorTM Kit V (Lonza) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 01F49i-N-B7 iPSCs were dissociated to single cells and 250,000 cells transfected with 25 μL of the prepared transfection mix containing 20 µL of nucleofection buffer (P3 Primary Cell 4D-NucleofectorTM X Kit S, Lonza), 5 µL of the RNP complex ...
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bioRxiv - Genomics 2022Quote: ... 5 μg of LMNA-VRQRABE-sgRNA was mixed in a total 25 μL volume (SE kit, Lonza) then resuspended with 200k HGPS cells and electroporated using the with CM-120 setting ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 105 cells were nucleofected (Amaxa® HUVEC Nucleofector® Program A-034, Kit #VAPB-1002, Lonza) with 50 nM of the following siRNA oligos ...
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bioRxiv - Neuroscience 2022Quote: ... 5 × 106 trypsinized cells were resuspended in 93.5 µl solution (mouse ES cell nucleofector kit, VPH-1001, Lonza). The solution includes 2 µg of each CRISPR/Cas9 vector (4 µg total ...
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bioRxiv - Immunology 2023Quote: ... The MPRA vector library was nucleofected into TPP macrophages (5µg vector into 5×106 cells) in 100μl nucleofection buffer (Human Macrophage Nucleofection Kit, Lonza) using a Nucleofector 2b (program Y-011) ...
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bioRxiv - Systems Biology 2020Quote: ... and 20 mg/L gentamicin (Lonza), and were differentiated into DCs by application of 800 U/mL GM-CSF (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... 20 mM Hepes (all from Lonza), 1 mg/ml Collagenase D (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... in 20-µL Nucleocuvette strips (Lonza). Cells were nucleofected using the program CM-125 or CM-130 set to P3 buffer ...
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bioRxiv - Physiology 2023Quote: ... and a 20% FBS BulletKit (Lonza) as described previously 24.
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bioRxiv - Cancer Biology 2019Quote: ... 2×105 cells were co-transfected with 2ug of the Cas9/sgRNA vector PxHF1* and 4 ul of ssODN HDR template (20 uM) using a Lonza X-Unit Nucleofector with P3 buffer kit (Lonza #V4XP-3032). Four days following transfection ...
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bioRxiv - Immunology 2020Quote: ... Transfection of primary T cells with Cas9 RNP complexes and TCRβα HDR templates was performed 3-4 days following activation using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Genomics 2021Quote: ... transfection of primary T cells with Cas9 RNP complexes and GSH1/GSH2-mRuby HDR templates was performed using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×106 EL16.7 TST ESCs were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001) using program A-30 with 1.6 µg each of GFP and tdTomato circularised targeting vectors and 5 µg single gRNA vector PX459 (5’-TATACCTAATCATTATGCCG-3’ ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of DNA was used to transfect the cells using the Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cas9-gRNA ribonucleoproteins were assembled as described previously53 and nucleofected into 5×106 monocytes in 100μL nucleofection buffer (Human Monocyte Nucleofection Kit, Lonza) using a Nucleofector 2b (Lonza ...
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bioRxiv - Immunology 2020Quote: ... resuspended in 20 μL solution SF (Lonza) and combined with 10 μM gRNA together with 0.5 μM GUIDE-seq dsDNA donor fragment ...
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bioRxiv - Immunology 2022Quote: ... and 20 μg/mL gentamicin sulfate (Lonza). Hi5 cells were cultured in Insect-XPRESS (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 20% fetal bovine serum (Lonza, Verviers, Belgium), at 37°C in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... and 20 µg ml-1 trypsin (Lonza). MDCK cells were inoculated at an moi of 0.01 ...
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bioRxiv - Cell Biology 2022Quote: ... 20 % v/v FCS (Lonza, Basel, Switzerland), 2 mM L-Glutamine ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... and Nucleofector device (program T-20, Lonza) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 20 ng/mL EGF (#CC-4107, Lonza), 0.5 mg/mL Hydrocortisone (#H4001 ...