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Citations for Lonza :
351 - 400 of 2158 citations for 20 Hydroxyecdysone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 10 µg donor DNA and 10 µg Cas12a crRNA template DNA were diluted in either 20 µl (Lonza transfection) or 50 µl (Tb-BSF transfection ...
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bioRxiv - Immunology 2019Quote: The Nucleofector transfection kit (Lonza) was used to transfect siRNA into cells ...
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bioRxiv - Microbiology 2023Quote: A ToxiLight BioAssay Kit (Lonza) was used to determine cell death ...
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bioRxiv - Microbiology 2023Quote: ... and Nucleofector Kit R (Lonza) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using ToxiLight bioassay kit (Lonza) according to the manufacture’s recommendations.
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were seeded in 6 well plates and grown to ∼80% confluency in DMEM (Lonza, 12-614F) with P/S ...
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bioRxiv - Cell Biology 2022Quote: ... cells were counted using Trypan Blue Exclusion Method and seeded into Collagen-I coated plates at a density of approximately 100,000 cells/cm2 following the Lonza’s instructions (Lonza, “Suspension and Plateable Cryopreserved Hepatocytes Technical Information and Instructions”) ...
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bioRxiv - Developmental Biology 2019Quote: ... HLECs were grown on firbonectin-coated plates or glass slide and were maintained in EBM2 media from Lonza. All experiments were conducted using passage 5-6 cells ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were passaged in poly-D-Lysine treated plates and incubated overnight in OPTI-MEM medium (Lonza) at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... HUVECs were prepared in 6-well plates with 2.8.105 cells/well in EGM-2 medium (Endothelial Cell Growth Medium-2, Lonza). Cells were transduced in duplicate with 20 MOI (Multiplicity of Infection ...
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bioRxiv - Immunology 2023Quote: ... 24-well plate) at a concentration of 75,000 cells per well in Smooth muscle growth medium (SmGM) (Lonza) and left to adhere and spread for 90 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... opaque 96-well culture plates and survival was measured using the ViaLight Assay (Lonza Group Ltd., Rockland, ME).
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bioRxiv - Immunology 2019Quote: ... and then cultured at 37°C under 5% CO2 in endothelial cell growth media (Lonza) for 2-3 days to become confluent 35 ...
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bioRxiv - Cancer Biology 2020Quote: ... spheres were counted by spinning at 300g for 5 minutes and suspending in PBS (Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... 5 μL of cell suspension in the same volume of Trypan-Blue (Lonza, LONZ17-942E) were counted in a Neubauer chamber.
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bioRxiv - Biophysics 2020Quote: CHO K1 cells were grown at 37 °C at 5% CO2 in DMEM/F12 (Lonza) supplemented with 10 % FBS and L-Glutamine (both Sigma) ...
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bioRxiv - Immunology 2022Quote: ... for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM, Lonza) supplemented with 5% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... centrifuged at 400xg for 5 min and resuspended in Hank’s Balanced Salt Solution (HBSS, Lonza). Paraformaldehyde (PFA ...
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bioRxiv - Immunology 2022Quote: ... 162 bp dsRNA with 5’ppp was electroporated into cells using a NucleofectorTM 2b (Lonza). 2 μg/ml of dsRNA was electroporated into cells using the cell line NucleofectorTM Kit L by following the manufacturer’s instructions using the U2OS program ...
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bioRxiv - Biophysics 2023Quote: ... 95% humidity and 5% CO2 in human hepatic stellate cell growth medium (MCST250, Lonza, Basel). 3 ml collagen solution (1.2 mg/ml ...
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bioRxiv - Biophysics 2023Quote: ... 95% humidity and 5% CO2 in human hepatic stellate cell growth medium (MCST250, Lonza, Basel).
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bioRxiv - Biophysics 2019Quote: ... These growth factors were supplied as a bullet kit (Cell Media and Bullet Kit, Lonza). HUVECs were passaged 2-6 times.
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bioRxiv - Cell Biology 2021Quote: ... Marrow cells were resuspended in 10 mL α – MEM and subjected to density centrifugation using 20 mL Lymphoprep™ (Lonza) at 800 x g for 20 minutes with no braking on the centrifuge ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed with 1X phosphate buffered saline (PBS) and resuspended in 20 µL of solution SF or SE (Lonza). Cell suspensions were combined with RNP complex(es) ...
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bioRxiv - Genomics 2019Quote: ... one million cells were mixed with 20 μg plasmids and 100 μL supplemented Nucleofector Solution V and electroporated with a Nucleofector II device (Lonza) using Program T-016 ...
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bioRxiv - Microbiology 2019Quote: ... 10 ug of Cas9 plasmid was combined with up to 80 ug of amplicon and resuspended in 20 uL P3 solution (Lonza), before being transfected by the Amaxa 4D Nucleofector (Lonza ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell suspension was further mixed with pre-formed Cas9 RNPs and equally distributed into two 20 µl cuvettes (V4XP-3024, Lonza). Nucleofection was performed using program EA104 ...
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bioRxiv - Genomics 2020Quote: ... A2lox ESCs (a gift from Michael Kyba) were transduced with 20 μg of each plasmid using the 4D-Nucleofector (Lonza), mouse ES program and P3 primary cell kit ...
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bioRxiv - Microbiology 2021Quote: 2×106 Elijah cells were washed in PBS and resuspended in 20 μl P3 Primary Cell Nucleofector Solution buffer prepared with Supplement 1 buffer (Lonza) according to the manufacturer’s instructions (P3 Primary Cell 4D-Nucleofector X Kit S) ...
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bioRxiv - Microbiology 2020Quote: ... The same number of cells (2 x 106 cells) were lysed in 100 µl of 1% CHAPS/PBS and analyzed on 4-20% SDS-PAGE gel (Lonza). The envelope proteins were detected by 2F5 (specific for gp41 ...
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bioRxiv - Neuroscience 2022Quote: ... and ssODN (1 μl,stock 100 μM) in 20 μl of nucleofection buffer P3 were nucleofected (program CA137, 4D-Nucleofector Device, Lonza) into 500,000 detached iPSCs (Deneault et al. ...
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bioRxiv - Biochemistry 2022Quote: ... the plasmids mEmerald-JUP-C-14 and mEmerald-Beta-Catenin-20 were transfected in 3T3-L1 pre-adipocytes by Nucleofection (Lonza). mEmerald-JUP-C-14 (Addgene plasmid # 54132 ...
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bioRxiv - Cancer Biology 2022Quote: ... and TILs were resuspended at a cell density of 1 million cells in 20 µL P3 electroporation buffer with supplement (Lonza) and then combined with 3 µL of RNP mixture and nucleofected using program DJ-110 (melanoma ...
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bioRxiv - Immunology 2020Quote: ... Rinsed cells were resuspended in buffer P4 (mouse) or P2 (human) at 1-10 x 106 cells/20 μL (Lonza). Non-targeting Alt-R crRNA #1 ...
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bioRxiv - Microbiology 2020Quote: ... and 3-fold (SARS-CoV-2 and SARS-CoV) serial dilutions of sample in serum-free EMEM supplemented with 20 µg/ml trypsin (Lonza) for MDCK cells ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were activated with plate-bound α-CD3 (3.75 µg/ml; Immunotech) and soluble α-CD28 (1 μg/mL; Immunotech) in X-vivo 20 serum-free medium (Lonza). X-vivo 20 medium was supplemented with L-glutamine (2 mM ...
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bioRxiv - Bioengineering 2019Quote: ... approximately 200,000 cells were resuspended in a mixture containing 20 µl of P3 Primary Cell Nucleofector™ solution (V4XP-3032, Lonza), 5 µl of RNP complex for each guide (both guides AC and AD ...
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bioRxiv - Bioengineering 2020Quote: DNA delivery was carried out on 20 μL volume format using Amaxa 4D-Nucleofector System and Cell line nucleofector solution SF (Lonza). Total amount of DNA electroporated was 3 μg (1.5 μg each plasmid in the case of using two plasmids at the same time) ...
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bioRxiv - Immunology 2020Quote: 2×106 freshly isolated primary B cells were washed in PBS and resuspended in 20 μl P3 Primary Cell Nucleofector Solution buffer prepared with Supplement 1 buffer (Lonza) according to the manufacturer’s instructions (P3 Primary Cell 4D-Nucleofector X Kit S) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were infected for 30 min with exponential-phase bacteria at a multiplicity of infection (MOI) of 2 and treated with 20 μg/ml of gentamicin (Lonza) for 60 min as described [22] ...
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bioRxiv - Cell Biology 2021Quote: ... the EV containing supernatant was filtered (0.45 μm) and transferred to TFF-Easy 20 kDa MWCO (HansaBioMed/Lonza, Tallinn, Estonia) for EV concentration ...
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bioRxiv - Biophysics 2021Quote: ... 2 μg of each Pcdh expression construct were transfected into 20 μL of the K562 cell suspension by electroporation using an Amaxa 4D- Nucleofector (Lonza). Transfected cells were transferred to a 24-well plate in 500 μL of medium per well and incubated overnight at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were conducted by running equivalent amounts of protein on 4-20% Tris-Glycine SDS/polyacrylamide gradient gels (Lonza) after reduction with 2-mercaptoethanol and heating to 95°C for 5m ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T-cells were stimulated with plate-bound α-CD3 (3.75 μg/ml; Immunotech) and soluble α-CD28 (1 μg/mL; Immunotech) in X-vivo 20 serum-free medium (Lonza). X-vivo 20 medium was supplemented with L-glutamine (2 mM ...
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bioRxiv - Immunology 2021Quote: ... the T cell / RNP / HDRT mixture was transferred onto a 16-well electroporation strip (20 µl = 106 cells per well, Lonza) or an electroporation cartridge (100 µl = 5×106 cells ...
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bioRxiv - Genomics 2021Quote: ... The EPCs were grown using EPC media (EGM-2MV supplemented with growth factors, ascorbic acid plus 20% Hyclone serum; CC-3202, Lonza and HYC-001-331G ...
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bioRxiv - Developmental Biology 2022Quote: 3 × 105 cells in 20 µL transfection buffer were mixed with 1µg plasmids and electroporated with a 4D nucleofector (Lonza Bioscience) following the preset protocol for K562 ...
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bioRxiv - Cancer Biology 2022Quote: ... The sgRNA and Cas9 ribonuclease protein complex under incubation was then mixed with the cell suspension and 20 µl of it was put a cuvette and nucleofected using 4D-Nucleofector (Lonza) with the inbuilt program DS-137 for cell lines and EO-115 for primary T cells ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of digested plasmid was added to the bottom of a 1.5 mL tube and 800,000 cells suspended in 20 µL SE nucleofection solution (16.4 µL nucleofection solution, 3.6 µL nucleofection supplement, Lonza, V4XC-1012). The solution was then transferred to a well in a 16-well Nucleocuvette™ Strip and nucleofected in a Lonza 4D-Nucleofector™ running the CM-104 program for U-2 OS cells ...
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bioRxiv - Immunology 2022Quote: ... 1×106 cells were electroporated (pulse code EH100) with Cas9 ribonucleoprotein and DNA templates in 20 ul Nucleofector Solution P3 (Lonza) with a 4D Nucleofector X unit (Lonza) ...