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Citations for Lonza :
4701 - 4750 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Sterile molten top NA (3 mL; 0.2 % SeaPlaque Agarose, Lonza) supplemented with CaCl2 and MgCl2 (both at 5 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 μg Tir1 targeting vector and 5 µg of pX330-EN1201) and transferred to a single Nucleocuvette (Lonza). Nucleofection was performed using the protocol CG110 ...
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bioRxiv - Molecular Biology 2022Quote: ... and the gRNA vector PX330-EN1082 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT) as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... Reaction mixtures were electroporated in 4D Nucleocuvettes (Lonza), and later recovered to culture dish wells containing pre-warmed media.
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bioRxiv - Molecular Biology 2022Quote: ... and cells were resuspended in buffer P3 (Lonza), in which 15 μL of buffer were used per one million T-cells.
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bioRxiv - Molecular Biology 2022Quote: ... transferred into the well of a 20 μL nucleocuvette (Lonza;). Cells were immediately nucleofected using the ‘Primary Cell P3’ program and ‘CA-137’ pulse code ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines were routinely tested for mycoplasma contamination using enzymatic (Lonza) and PCR-based assays (Bulldog Bio).
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bioRxiv - Molecular Biology 2022Quote: ... D-PBS was aspirated and cells were resuspended in 15 μL buffer P3 (Lonza). The cell suspension was then transferred to the RNP mix and thoroughly triturated in the RNP mix ...
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bioRxiv - Molecular Biology 2022Quote: ... was transfected with the targeting vector pUC19-ITR-NeoR-ITR-3xCTCF-LacO and the gRNA vector pX330-chr15_LacO_gRNA/Cas9 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT) as described for the Tir1 integration ...
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bioRxiv - Molecular Biology 2022Quote: ... using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT). 2×106 cells were harvested using accutase (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with the targeting vector pMK-3xCTCF-TetO-Rox-PuroR-Rox and the gRNA vector PX459-chr15_gRNA/Cas9 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT). 2×106 cells were nucleofected with 1 μg TetO targeting vector and 1 µg of PX459-ch15_gRNA/Cas9 ...
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bioRxiv - Molecular Biology 2022Quote: ... D-PBS was gently aspirated from the T cell pellet and then resuspended in 15 μL of buffer P3 (Lonza). The cell suspension was then transferred to the RNP mix and thoroughly triturated ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoresis of 10 µl of RT-PCR products was performed using 3% agarose (SeaKem LE Agarose by Lonza) with molecular ladder gel containing 0.5 µg/ml ethidium bromide in x0.5 tris-acetate-ethylenediaminetetraacetic acid (TAE ...
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bioRxiv - Microbiology 2022Quote: ... and transfected using an Amaxa Biosystems Nucleofector II (Lonza, Cologne, Germany) set to program U-33 ...
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bioRxiv - Microbiology 2022Quote: ... dissolved in Basal Epithelial Cell Growth Medium (BEBM, Lonza)) ...
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bioRxiv - Microbiology 2022Quote: ... BEAS-2B cells were cultured in Bronchial Epithelial Cell Growth Medium (BEGM, Lonza, Kingston, ON, Canada) on plates prepared with coating media (0.01 mg/ml fibronectin ...
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bioRxiv - Immunology 2022Quote: ... THP-1 cells were grown in suspension culture using RPMI-1640 media (Lonza) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... and then transferred by the N-024 program of Amaxa’s Nucleofector electrotometer (Lonza, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and the well was gently washed once with 5 mL of PBS (Lonza, Rockland) to remove un-adherent floating bacteria ...
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bioRxiv - Microbiology 2022Quote: ... and the well was gently washed once with 5 mL of phosphate buffered saline (PBS, Lonza, Rockland, ME, USA) to remove un-adherent floating bacteria ...
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bioRxiv - Microbiology 2022Quote: ... Nuli-1 cells were cultured on Collagen IV coated plates as previously described in Bronchial Epithelial Cell Growth Media (BEGM) with supplements (Lonza) (Zabner et al. ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% FBS and 1% NuSieve Agarose (Lonza, 12001-722). Plaques were allowed to develop for 10 days and total number of plaques in each well was counted using a fluorescent microscope ...
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bioRxiv - Genomics 2022Quote: ... cells were grown and passaged in complete Endopan-2 medium (Lonza) until 90% confluency and scraped in PB ...
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bioRxiv - Genomics 2022Quote: ... donors (passage 4-9; Lonza) were grown in complete Endopan-3 medium kit (PAN-Biotech ...
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bioRxiv - Genomics 2022Quote: ... Following 6-TG treatment 5×106 HM-1 cells were transfected using a Nucleofector®-4D (Lonza) with the P3 Primary Cell X kit ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed using Lonza ACK Lysing buffer (Lonza, Basel, Switzerland). Splenocytes were stained with LCMV-specific gp-33 tetramer and surface antibodies then fixed overnight with FOXP3 Fixation/Permeabilization reagent (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... was performed by nucleofection of primary cDCs isolated from HD with specific siRNAs (SMART-pool, Horizon Discovery) or irrelevant scramble siRNA in an Amaxa4D-Nucleofector (Lonza) instrument using the CM120 protocol and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin and 1% Ultra-glutamine (both from Lonza) RPMI 1640 media ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin (Lonza) and 1% Ultra-glutamine (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... Cell lines were regularly tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza). CARD8−/− ...
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bioRxiv - Immunology 2022Quote: ... Cell lines were regularly tested for mycoplasma using the MycoAlert™ Mycoplasma Detection Kit (Lonza).
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bioRxiv - Immunology 2022Quote: ... Cell lines were regularly tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza). CARD8−/− ...
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bioRxiv - Immunology 2022Quote: ... 24 mM Sodium Succinate) using Amaxa™ 4D-Nucleofector™(Lonza). Electroporated B cells were culture in 200ul primary B cell medium for 6 days.
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bioRxiv - Synthetic Biology 2022Quote: ... were ligated to the target fragments and full-length ligation products were purified via gel electrophoresis (2% MetaPhor agarose, Lonza, Basel, Switzerland). Purity and concentration of extracted fragments were determined using capillary electrophoresis (Fragment Analyser ...
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bioRxiv - Zoology 2022Quote: ... titanium dioxide and gellan gum (Lonza Australia, #G69CS000753). The enteric coating prevented bait moisture from dissolving the capsules ...
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bioRxiv - Synthetic Biology 2022Quote: ... T cells were resuspended in P3 buffer (0.75-1 x 106 cells per 18 μl P3) from the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). 10 μg/μl Alt-R S.p ...
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bioRxiv - Microbiology 2022Quote: ... diluted in an equal volume of DMEM containing 20% FBS and 200 U/ml of penicillin/streptomycin (Lonza), and ∼1.5×104 hemolymph sporozoites were added per well ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were counted using trypan blue stain (Lonza Bioscience). For the paraquat proliferation assays ...
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bioRxiv - Microbiology 2022Quote: ... at 200 µg/ml and incubated at 37°C overnight in DMEM (Gibco or Lonza) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: Transfection of primary cortical cells was performed using nucleofection (Lonza Bioscience) according to our previously published protocol (26).
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bioRxiv - Microbiology 2022Quote: ... supernatants were diluted 20-50 times before a Limulus amoebocyte (LAL) kinetic-QCL assay was applied according to the manufacturer’s instructions (Lonza Ltd., Basel, CH). The detection limit of the assay was estimated at 0.5 -2.5 EU/filter depending on the dilution rate ...
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bioRxiv - Microbiology 2022Quote: ... Human bronchial epithelial cells (NuLi-1) were cultured as previously described (19) in BEGM (Lonza, USA) with 1.15 mM calcium chloride and maintained as for hTCEpi ...
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bioRxiv - Microbiology 2022Quote: Human corneal epithelial cells (hTCEpi) (67) were cultured in KGM-2 (Lonza, USA) supplemented with 1.15 mM calcium chloride (high-calcium ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells (Lonza, Allendale, NJ, USA) were cultured in bronchial epithelial cell growth medium (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... Cells were maintained in RPMI-1640 medium (Lonza, Swiss) supplemented with 10% dFCS ...
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bioRxiv - Microbiology 2022Quote: ... 3 to 4×107 cells were transfected with 3 to 8 μg of DNA in 100 μl bloodstream form transfection buffer (Schumann Burkard, 2011) using program Z-001 on the Amaxa Nucleofector (Lonza). Limiting dilutions of the parasites were prepared in 48-well plates ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... crRNPs for each gene of interest were generated as described previously in the Knockout Cell Clones and Pools section except that supplemented P3 buffer (Lonza; V4SP-3096) is used instead of SE buffer ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The resuspended cells were immediately transferred into the cuvette of the P3 Primary Cell Nucleofector Kit (Lonza; V4SP-3096) and electroporated using code EH-100 on the Lonza 4D-Nucleofector ...
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bioRxiv - Microbiology 2022Quote: ... the QCL-1000 Assay (Lonza Walkersville ...
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bioRxiv - Microbiology 2022Quote: ... and 50 U/mL penicillin/streptomycin (Lonza). HEK293TT cells were kindly provided by Prof Greg Towers (University College London (UCL) ...