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Citations for Lonza :
4651 - 4700 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: A single cut in the NKX2.2 3’UTR was made using transient transfection (Lonza Human Stem Cell Nucleofector Kit VPH-5012 ...
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bioRxiv - Immunology 2022Quote: ... for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM, Lonza) supplemented with 5% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Macrophage culture was performed under entirely serum-free conditions throughout using X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) supplemented with 1% penicillin-streptomycin (Cat ...
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bioRxiv - Immunology 2022Quote: ... PBMC were seeded at 1.5×106 cells per cm2 in X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) +1% penicillin-streptomycin (Cat ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 25μL of SEC fractions 1 to 5 diluted 1:2 with PBS (Cat. No. LZBE17-512F, Lonza). Wells were washed with 0.05% Tween-20 (Cat ...
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bioRxiv - Immunology 2022Quote: All cell lines were STR profiled and tested for Mycoplasma using the MycoAlert kit (Lonza).
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bioRxiv - Immunology 2022Quote: ... sodium pyruvate (Lonza), amino acid (NEAA 100x Lonza ...
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bioRxiv - Genomics 2022Quote: ... 1X non-essential amino acids (Lonza), 1X Glutamax ...
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bioRxiv - Synthetic Biology 2022Quote: 1 x 106 BHK-21 cells were electroporated with a total of 7.8 μg of mRNA (1:1:1 of pSinHelper, pSinCapsid and pTSin-EGFP/pTSin-SRF-NLS-VP64) using Amaxa 2B (Lonza) as per the manufacturer’s instructions for BHK-21 cells ...
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bioRxiv - Physiology 2022Quote: ... 1 million cells were washed with PBS and electroporated with 5-10 μg of appropriate plasmid using Amaxa cell nucleofector kit T (Lonza laboratories). Cells were allowed to recover for 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... and Human CD34+ Cell Nucleofector Kit (Lonza) using a Nucleofector 2b Device (Lonza) ...
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bioRxiv - Neuroscience 2022Quote: ... The OPC cell cultures were regularly tested to be mycoplasma free using a MycoAlert Mycoplasma Detention Kit (Lonza, LT07-118). The OPCs were authenticated with immunopositivity for OPC cell markers (Nkx2—2 ...
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bioRxiv - Neuroscience 2022Quote: ... using a Nucleofector 2b Device (Lonza). After electroporation cells were cultured on Matrigel coated 6-well plates (100 µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 500 ng of the extracted DNA samples were run on 0.75% Seakem® gold agarose gel (Lonza Bioscience, Basel, Switzerland) for 16 hours on Pippin Pulse Electrophoresis Power Supply System (Sage Science ...
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bioRxiv - Microbiology 2022Quote: ... Sections attached to slides were fixed with cold acetone/ethanol (1:1) solution and washed in PBS buffer (Lonza, Morristown, NJ). Primary antibodies and isotype controls (Supplementary Table 9 ...
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bioRxiv - Microbiology 2022Quote: ... were cultured in Dulbecco's modified Eagle’s medium with glutamine (DMEM, Lonza) supplemented with 10% FBS (Gibco® ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from Promocell and passaged in EGM2 medium (Lonza) for up to 5 passages ...
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bioRxiv - Microbiology 2022Quote: ... 1X Insulin-Transferrin-Ethanolamine-Selenium (ITES; Lonza, cat. no. 17-839Z), 20 nM triiodothyronine (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The channels were filled with 2% agarose (Lonza, cat. no. 50302) and the whole chip was embedded in O.C.T ...
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bioRxiv - Microbiology 2022Quote: The resulting plasmid pool was linearised and transfected into 107 PRUΔHXGPRT tachyzoites using the Amaxa 4D Nucleofector system (Lonza) with buffer P3 and pulse code EO-115 ...
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bioRxiv - Microbiology 2022Quote: ... was co-transfected with 15 μg pCas9 plasmid targeting the gene of interest into the RHΔKU80 and PRUΔKU80 lines using the Amaxa 4D Nucleofector system (Lonza) as above ...
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bioRxiv - Immunology 2022Quote: ... blood was isolated using the retroorbital route and red blood cells lysed twice using Lonza ACK Lysing buffer (Lonza, Basel, Switzerland), then subjected to staining as described above ...
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bioRxiv - Physiology 2022Quote: ... 2 mM L-glutamine (Lonza, UK), 24 μg/mL adenine ...
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bioRxiv - Physiology 2022Quote: ... (5 g/L trypsin and 2 g/L EDTA diluted 1 in 5 in calcium- and magnesium-free PBS) (Lonza, UK).
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bioRxiv - Physiology 2022Quote: ... 5 million cells were washed with phosphate buffered saline (PBS) and electroporated with 4-6 μg of appropriate plasmid construct using an Amaxa cell nucleofector (Lonza laboratories) and nucleofection reagent (362.88 mM ATP-disodium salt ...
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bioRxiv - Physiology 2022Quote: Isolated EVs were diluted with PBS (Lonza) and loaded onto formvar-carbon–coated copper grids ...
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bioRxiv - Neuroscience 2022Quote: ... Nucleofected striatal neurons were transfected using manufacturer’s instructions (Rat Neuron Nucleofector Kit, Lonza) for rat hippocampal neurons before plating ...
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bioRxiv - Neuroscience 2022Quote: ... diluted in 0.1M Dulbecco’s Phosphate Buffered Saline (PBS) solution (Lonza, Cat# 12001-664). The slices stayed in the 4% PFA solution for 24-48 hours before being places in a 0.1M PBS solution until histology was performed.
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel ...
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bioRxiv - Physiology 2022Quote: ... 2 mM L-glutamine (Lonza, UK), 100 IU/mL penicillin,100μg/mL streptomycin ...
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bioRxiv - Physiology 2022Quote: ... in F12 : DMEM (1 : 1) (Lonza, UK). Following digestion the suspension was passed through sterile gauze and a 70 µm cell strainer before centrifugation at 350 g for 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... washed with PBS (Lonza, UK), re-suspended in 400 µL of PBS and data obtained immediately on a Cytoflex flow cytometer (Beckman Coulter Life Sciences) ...
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bioRxiv - Physiology 2022Quote: ... and 2.5 μg/mL amphotericin B in Hank’s balanced salt solution (HBSS) (Lonza, UK). It was then finely minced and digested overnight on a rocker at 4 °C in 1 mg/mL protease XIV (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... 0.005% trypsin (Lonza, UK), and 10 ng/mL DNAse I (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... The final EVs pellets were suspended in 200 µl of PBS (Lonza). Samples were aliquoted and stored at -80°C for further analysis.
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bioRxiv - Physiology 2022Quote: ... Samples were diluted in PBS (Lonza) to reach a particle concentration suitable for unbiased analysis ...
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bioRxiv - Genomics 2022Quote: ... All cell lines were regularly screened for mycoplasma contamination using the MycoAlert kit (Lonza).
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bioRxiv - Immunology 2022Quote: ... red blood cells were lysed using ACK lysis buffer (Lonza, Cat# BP10-548E) and the remaining cells were quantified using an automated cell counter (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were overlaid with 100 μl/well of the 1:1 (v/v) mixture of 2.4% Avicel (DuPont) and 2XEMEM (Lonza) with 4% FBS ...
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bioRxiv - Microbiology 2022Quote: ... mixed with DNA and/or RNA as indicated in each experiment and electroporated at 110 V for 10 ms by the electroporator (Lonza Amaxa Nucleofector 2B). After electroporation ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid DNA composed of a 1:1 mixture of the targeting and the donor vectors was resuspended in 100 μL of P3 buffer (Lonza), containing 3 μL ATP (625 mM) ...
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bioRxiv - Microbiology 2022Quote: ... Digested products were then visualised on a 4% NuSieve (Lonza, 50090) agarose gel with SafeView Nucleic Acid Stain.
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... The supernatant solution was collected and the DNA library was confirmed by Flashgel (Lonza, catalog no. 57023), and then purified using a Zymo DNA Clean& Concentrator™-5 kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... HUVEC are primary endothelial cells obtained from a single female donor (Lonza). Both HPMEC and HUVEC cell lines were propagated (passages 5–10 ...
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bioRxiv - Microbiology 2022Quote: ... Both HPMEC and HUVEC cell lines were propagated (passages 5–10) and maintained in endothelial growth medium 2 (EGM-2) using the EGM-2 bullet kit from Lonza following the manufacturer’s specifications and grown in a CO2 incubator at 37°C with 5% CO2.
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bioRxiv - Microbiology 2022Quote: ... The cells were nucleofected using 4D Nucleofector LV Unit (Lonza) and collected in 5 mL of complete growth medium ...
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bioRxiv - Microbiology 2022Quote: ... Pelleted cells were resuspended in SF Cell Line Nucleofector Solution with added supplement (Lonza catalog # V4XC2024). 25 µg of the plasmid to be transfected (Aldevron ...
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bioRxiv - Microbiology 2022Quote: ... the pellets were resuspended in 20 mL of P3 solution with added supplement (Lonza), and 2 mg of the plasmid encoding tandem copies of the LY2 genome (Aldevron ...
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bioRxiv - Microbiology 2022Quote: ... was added to the resuspended cells and nucleofected using the CM-150 program on the 4D-Nucleofector X Unit (Lonza). Nucleofected cells were allowed to recover in a 37°C incubator with 5% CO2 for 20 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... HAECs were cultured in EBM-2 supplemented with singleQuots (LONZA, Clonetics CC-4176) and Endothelial Cell Growth Kit-VEGF (ATCC ...