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Citations for Lonza :
401 - 450 of 509 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Macrophage culture was performed under entirely serum-free conditions throughout using X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) supplemented with 1% penicillin-streptomycin (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... The OPC cell cultures were regularly tested to be mycoplasma free using a MycoAlert Mycoplasma Detention Kit (Lonza, LT07-118). The OPCs were authenticated with immunopositivity for OPC cell markers (Nkx2—2 ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines used in this study were routinely tested for mycoplasma and found to be mycoplasma-free (MycoAlert Mycoplasma Detection Kit MycoAlert, Lonza). Flp-In™ T-REx™ 293 cells were cultured according to the manufacturer’s protocol.
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bioRxiv - Physiology 2022Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10μM BrdU ...
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bioRxiv - Physiology 2024Quote: ... of six genetically independent donors were grown as monolayers in 100% humidity and 5% CO2 at 37 1C in serum-free defined growth media (BEGM, Lonza). NHBEs (passage 3 ...
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bioRxiv - Molecular Biology 2024Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10µM BrdU ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were confirmed to be mycoplasma-free based on a luminescence-based enzymatic assay (MycoAlert®, Lonza, Cat# LT07-118) conducted within 1 week of the commencement and completion of the experiments ...
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bioRxiv - Developmental Biology 2023Quote: ... were individually index sorted to each well of 96-well containing FL-AKT-EC in serum-free coculture media consisting of X-VIVO 20 (Lonza) with recombinant cytokines (PeproTech) ...
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bioRxiv - Bioengineering 2023Quote: ... a second biopsy from a colposcopically abnormal region of the cervix was taken and placed in a custom-built tissue carrier containing keratinocyte serum-free media (KSFM; Lonza). Biopsies were transported via personal vehicle to the Tufts Advanced Microscopy Imaging Center (TAMIC ...
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bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
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bioRxiv - Immunology 2023Quote: ... CTLs derived from LifeAct-GFP expressing pmel-1 mice were incubated with peptide-loaded and IFNγ-treated B16 cells cultured in phenol red-free DMEM (Lonza) supplemented with 10% FBS on stage at 37°C ...
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bioRxiv - Genomics 2023Quote: ... All cell lines were authenticated by short tandem repeat (STR) profiling and verified mycoplasma free using the MycoAlert PLUS Mycoplasma Detection kit (Lonza) prior to conducting experiments ...
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bioRxiv - Biochemistry 2023Quote: ... Sf21 cells seeded at 500,000 cells/mL were transfected with recombinant bacmid DNA to generate the initial baculovirus (P1) in Insect-XPRESSTM Protein-free Insect Cell Medium (Lonza). The P1 virus was amplified two times (P2 and P3 ...
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bioRxiv - Immunology 2024Quote: ... insert and placed in one well of a six-well plate in 1 ml serum free culture medium (“RB27”) composed of RPMI 1640 (Lonza), 4% B27 supplement (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... with a density of 1.5 x106 using a 1:70 inoculation from the V1 stock for 92 hours at 21°C in Insect Xpress Protein-free Insect Cell Medium (Lonza) supplemented with GlutaMAX (GIBCO ...
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bioRxiv - Molecular Biology 2024Quote: All cells were cultured at 37°C in 5% CO2 in humidified incubators and were free from mycoplasma (MycoAlert Detection Kit, Lonza). Human primary hepatic stellate cells used for the main experiments were from Lonza (HUCLS ...
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bioRxiv - Bioengineering 2024Quote: ... in a specimen cup containing a custom-built tissue carrier and a small volume of keratinocyte serum-free medium (Lonza) to provide protection and physiologically relevant hydration ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.2% amphotericin B (all from Life TechnologiesCell cultures were tested and confirmed to be free from mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza). SFs from passages 4 to 7 were used for the experiments.
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bioRxiv - Immunology 2024Quote: ... PBMCs were cultured at 37°C with 5% CO2 under humidified atmosphere and differentiated into monocyte derived dendritic cells (moDCs) using X-Vivo 15 Serum-free Hematopoietic Cell Medium (Lonza) medium supplemented with human GM-CSF (Gentaur) ...
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bioRxiv - Immunology 2024Quote: ... PBMC vials were thawed in the 37 °C water bath for 1-2 minutes and resuspended in warm X-VIVO 10 serum-free cell-media (Lonza). Samples were washed by centrifugation for 12 minutes at 1200 rpm at room temperature (RT) ...
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bioRxiv - Bioengineering 2024Quote: ... The cell lines were propagated in antibiotic free medium and were monitored regularly for mycoplasma infection using MycoAlertTM PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Biophysics 2024Quote: ... and T24 cells (gift from Prof. M. Sokabe of Nagoya University),81 confirmed free of mycoplasma contamination by MycoAlert (Lonza), were routinely cultured in Ham’s Nutrient Mixture F12 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... All cells were cultured at 37°C in 5% CO2 in humidified incubators and were free from mycoplasma (MycoAlert Detection Kit, Lonza).
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bioRxiv - Cell Biology 2020Quote: ... containing bone marrow mononuclear cells was washed in basal medium (α-MEM containing 10% FBS and 100 U mL-1 penicillin and 100 µg mL-1 streptomycin; Lonza). Cells were subsequently either sorted using MACS or incubated with SNAs.
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bioRxiv - Molecular Biology 2022Quote: ... Percoll-purified (82) late-schizont-stage parasites were transfected with 50 µg of plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described(83) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Percoll-purified [131] parasites at late schizont stage were transfected with 50 µg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described [135] ...
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bioRxiv - Biochemistry 2024Quote: ... Stable cell lines containing an empty vector or HA-labeled H2B variant were derived by electroporating ∼1 x 106 MCF10A cells and 2 µg of the minigene plasmid with a Lonza 4D-Nucleofector® X Unit (Lonza) and SF Cell Line 4D-Nucleofector™ X Kit S (Lonza ...
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bioRxiv - Genomics 2021Quote: ... two million H9 hESCs were co-electroporated with the appropriate knockin vector (5 μg) and plasmids encoding AAVS1-targeting TALENs (2 μg; addgene, 59025 and 59026) using an Amaxa 4D Nucleofector system (Lonza). Serial cell dilutions were then seeded in six-well plates in E8 supplemented with Y-27632 (10 μM) ...
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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bioRxiv - Immunology 2022Quote: ... Lysate were pellet (500 g 8min) and red blood cells were lysate with 500 µl of ACK lysing buffer (Lonza) for 5min ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle Medium 4.5 g/L glucose w/L-Gln w/ sodium pyruvate (DMEM, Lonza, Maryland, USA), and 10% fetal calf serum ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 μg of plasmid DNA was introduced to 4×106 neurons using an AMAXA nucleofection kit (VPG-1001, VPG-1003; Lonza). AMAXA-nucleofected cells were plated in 35mm glass bottom imaging dishes ...
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bioRxiv - Biophysics 2022Quote: ... The transfected cells were then co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) and cultured until virus-mediated CPE was observed ...
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bioRxiv - Synthetic Biology 2022Quote: 1 x 106 BHK-21 cells were electroporated with a total of 7.8 μg of mRNA (1:1:1 of pSinHelper, pSinCapsid and pTSin-EGFP/pTSin-SRF-NLS-VP64) using Amaxa 2B (Lonza) as per the manufacturer’s instructions for BHK-21 cells ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow cells were recovered by flushing out the bone marrow using a 22 G needle in complete RPMI medium (RMPIc): RPMI (Lonza) with 10% v/v heat inactivated foetal calf serum (HI-FCS ...
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bioRxiv - Cell Biology 2022Quote: ... Two million of MNCs were transfected with 10 μg of plasmid (5:1 ratio pEB-C5:pEBTg) as instructed by the Lonza CD34+ nucleofector kit (VPA-1003, Lonza) and Amaxa nucleofector (program T-016 ...
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bioRxiv - Immunology 2022Quote: Sorted CD8+ T cells with low and high ΔYm were centrifuged at 200 × g for 10 minutes prior to nucleofection (4D nucleofector system, Lonza). Three-five million cells were combined with 200 pMol siRNA (siControl or siME1 ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 107 epimastigotes in 0.5 mL were incubated with 30 μg of the plasmids in the buffer as described [101] and pulsed twice with the AMAXA Nucleofactor II electroporator (Lonza) X-014 program ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa and SH-SY5Y cells were grown in Dulbecco’s modified Eagle medium with L-glutamine and 4.5 g/L glucose (DMEM; Lonza or Biowest) supplemented with 10% fetal calf serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 10 mL of exponential culture was centrifuged (3250 g, 25 min) and the pellet was resuspended in the same volume of PBS (Lonza) with addition of 10 µM CFSE (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... with 2 μg pMito-iRFP670-FRB and 1 μg of the required GFP–FKBP (Ctrl or -Rab25) using programme A-23 (Lonza). The following day ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 106 cells were centrifuged at 90 x g for 10 minutes and then resuspended in 100 μl nucleofector solution V (Lonza). The cells were combined with 2000 ng plasmid DNA and placed in a specially designed cuvette ...
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bioRxiv - Genomics 2021Quote: ... Four aliquots of one million cells each per study subject were stimulated overnight for 12 hours in 1 ml X-VIVO™ 15 Serum-free Hematopoietic Cell Medium (Lonza) with 10 ul ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) ...
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bioRxiv - Immunology 2021Quote: ... The frequency of cells producing IFN-γ in responses to antigen was quantified with ELISpot (Diaclone; 2B Scientific, Oxon, U.K.) performed in HL-1 serum-free medium (BioWhittaker; Lonza, Slough, U.K.), supplemented with L-glutamine and penicillin–streptomycin (Life Technologies ...
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bioRxiv - Bioengineering 2020Quote: 2 million UCB-MNC/mL were cultured in X-VIVO 15 serum-free cell-culture medium (Lonza Group Ltd, Basel, Switzerland), supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Microbiology 2021Quote: ... Monocytes were isolated by adherence to plastic and then cultured for one week in X-VIVO 15 serum-free medium (Lonza, Inc.) and 20 ng / mL recombinant human GM-CSF (300-03 ...
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bioRxiv - Cancer Biology 2021Quote: All cell lines used in this study were confirmed mycoplasma-free by an in-house testing regimen (MycoAlert, Lonza; LT07-703). MDA-MB-231 cells from the American Type Culture Collection (ATCC ...
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bioRxiv - Immunology 2021Quote: ... The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza, Walkersville, MD, USA), in the absence or presence of 100 U/ml penicillin ...