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Citations for Lonza :
351 - 400 of 509 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: 106 freshly lysed parasites were transfected with 20-25 µg of DNA by electroporation using the 4D-Nucleofector (Lonza) with previously optimised protocols as described in Young et al (2) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Dulbecco’s modified eagle media (DMEM) with 4.5 g/L glucose and L-glutamine was from Lonza (Basel, Switzerland). Minimal Essential Media (MEM ...
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bioRxiv - Cell Biology 2024Quote: ... STD-M (−) consisted of Dulbecco’s Modified Eagles Medium 4.5 g/L D-glucose (DMEM; Lonza, Walkersville, MD, USA) supplemented with 50 U/mL penicillin (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... The product was purified and 2 µg were added to the RNP and diluted in 100 µL P3 nucleofection buffer (Lonza). This mixture was nucleofected with 2×106 stimulated human primary T cells using the 4D-Nucleofector (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell lysate containing tau seeds was combined with the Tau(P301S)-YFP cells at 1.8 µg/mL and electroporation was performed on an Amaxa Nucleofactor instrument (Lonza Bioscience) using the program #Q-001 ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 NSCs were mixed with 5 µg of corresponding DNA with the Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) and pulse was delivered using the CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Biophysics 2022Quote: ... 4-6 µg of DNA was mixed with the resuspended cells and electroporated using the Amaxa cell nucleofector (Lonza Laboratories) program Q-001 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of plasmids were mixed with 5 millions ESC in buffer “Mouse ES cell nucleofector kit (Lonza, VPH-1001) per electroporation ...
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bioRxiv - Molecular Biology 2021Quote: ... Percoll-purified57 parasites at late schizont stage were transfected with 50 µg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described65 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were co-transfected with 2 µg of the CRISPR/Cas9 plasmid and 2 µg of ssODN (50 µM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Two million E14 ESCs were nucleofected with paired Kdm3b targeting and donor plasmids (5 µg each) using the Amaxa 4D-Nucleofector protocol (Lonza) and selected with blasticidin S hydrochloride (Research Products International ...
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bioRxiv - Systems Biology 2024Quote: The reporter cell line for the PTGR screen was created by nucleofection of haploid AN3-12 mESCs with 500ng of the reporter construct and 10 µg of a Tol2 transposase encoding plasmid using the Mouse ES Cell Nucleofector Kit (Lonza) according to the manufacturer’s protocol using an Amaxa Nucleofector (Lonza) ...
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bioRxiv - Microbiology 2023Quote: ... Stable cell lines were generated by nucleofecting 2 µg of pBud-PCID2-Flag plasmid or empty pBud as control using Amaxa Nucleofector (Lonza) and Nucleofector Kit R (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... reprogramming was initiated by nucleofection of 1×105 fibroblast with 1 µg of each episomal plasmid (pCXLE-hUL, pCXLE-hSK and pCXLE-hOCT4) using the Nucleofector 2b (Lonza). Initially ...
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bioRxiv - Physiology 2024Quote: ... counted and 3.106 cells resuspended in 100 μl Nucleofactor R solution and electroporated with 3 µg of plasmid (pcDNA3 containing or not α7-5HT3 cDNA) using the Amaxa nucleofactor kit R (Lonza) according to the manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza). For the CLEM experiments the cells were transfected with TSP-1-GFPSpark and TSP-4-mCherry ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µg from all conditions were simultaneously concentrated by 10 % SDS-PAGE and visualized by Sypro-Ruby fluorescent staining (Lonza). Gel slices were excised and ...
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bioRxiv - Immunology 2020Quote: ... All cells were confirmed to be free of mycoplasmas before injection into mice by the MycoAlert detection kit (Lonza). Tumor growth was monitored using an electronic caliper and volumes were determined using the following formula ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were washed 3-4 times over 1 hour in PBS (calcium- and magnesium-free; Lonza BioWhittaker #17-517Q) containing 0.1% Triton X-100 (PBT ...
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bioRxiv - Molecular Biology 2022Quote: ... All cells were free of Mycoplasma contamination as confirmed by routine testing using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Immunology 2020Quote: ... DCs were seeded in a 24-well plate at a density of 1× 106 cells/well on 1.5mm coverslips (Warner Instrument) and cultured in complete phenol-red free RPMI 1640 media (Lonza) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... was adjusted to OD600 of 2.5 (2.2 × 109 bacteria/mL) and the bacteria were then further diluted in serum-free XVIVO-15 medium (Lonza) prior to infection to obtain the respective multiplicity of infection (MOI) ...
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bioRxiv - Immunology 2020Quote: ... All cell lines were tested and confirmed to be Mycoplasma and endotoxin-free using the MycoAlert Detection kit (Lonza) and the Endosafe-PTS system (Charles River Laboratories) ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were free from mycoplasma contamination after testing with the MycoAlert™ PLUS mycoplasma detection kit (Lonza) (LT07-701).
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were cultured at 5% CO2/37°C in serum-free X-Vivo 15 medium (Lonza).
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% tetracycline-free and heat inactivated fetal bovine serum (FBS) and 100 U/mL penicillin/streptomycin (Lonza). Cell lines were grown at 37 °C in a humidified atmosphere of 5% CO2 and were tested for absence of mycoplasma contamination.
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bioRxiv - Cell Biology 2022Quote: ... HLFs were seeded in four wells of a 24-well plate in antibiotic free medium containing 2 % (Lonza, PromoCell) or 10 % (DZL ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting vector was used to generate a D.mel-2 stable cell line adapted to grow in suspension in serum-free Insect-Xpress medium (Lonza). In brief ...
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bioRxiv - Immunology 2024Quote: ... plus memory T-cell (1000-4000 cells) in 200μl of X-VIVO 15 Serum-free Cell Medium (BE02-060F, Lonza) supplemented with 2% human serum (H3667 ...
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bioRxiv - Cell Biology 2024Quote: ... All the cell lines were confirmed to be free of mycoplasma contamination by using a Mycoalert PLUS kit (Lonza). To inhibit transcription ...
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bioRxiv - Genetics 2024Quote: ... Naïve T cells were cultured in basal media comprised of X-VIVO 15 Serum-free Hematopoietic Cell Medium (Lonza) with 5% FBS (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG, G-013 program; Lonza) before plating and maintained for 48 h.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested with trypsin, pelleted (500 g, 5 min, 4°C) and resuspended in PBS (pH 7.4) containing EDTA (Lonza) and 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested with trypsin, pelleted (500 g, 5 min, 4°C) and resuspended in PBS (pH 7.4) containing EDTA (Lonza) and 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM with 4.5 g/L glucose and L-glutamine) from Lonza (Basel, Switzerland) with 10 % v/v fetal bovine serum (Gibco 16000-044) ...
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bioRxiv - Cell Biology 2020Quote: ... containing bone marrow mononuclear cells was washed in basal medium (α-MEM containing 10% FBS and 100 U mL-1 penicillin and 100 µg mL-1 streptomycin; Lonza). Cells were subsequently either sorted using MACS or incubated with SNAs.
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bioRxiv - Molecular Biology 2022Quote: ... Percoll-purified (82) late-schizont-stage parasites were transfected with 50 µg of plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described(83) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Percoll-purified [131] parasites at late schizont stage were transfected with 50 µg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described [135] ...
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bioRxiv - Biochemistry 2024Quote: ... Stable cell lines containing an empty vector or HA-labeled H2B variant were derived by electroporating ∼1 x 106 MCF10A cells and 2 µg of the minigene plasmid with a Lonza 4D-Nucleofector® X Unit (Lonza) and SF Cell Line 4D-Nucleofector™ X Kit S (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Thawed PBMCS were rested for 3-4h at 37°C in X-VIVO 15 Serum-free Hematopoietic Cell Medium (Lonza) supplemented with 5% human Ab serum ...
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bioRxiv - Bioengineering 2021Quote: ... Each chamber was connected to a reservoir that was pre-filled with 15 mL of warm serum-free EGM-2 (endothelial cell growth medium-2 without serum added from the kit, Lonza). ECFCs were harvested and resuspended in serum-free EGM-2 and added to the reservoirs to reach an overall cell density of 125,000 cells/mL ...
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bioRxiv - Immunology 2021Quote: ... of four genetically independent donors were grown as monolayers in 100% humidity and 5% CO2 at 37 °C in serum-free defined growth media (BEGM, Lonza). NHBEs (passage 3 ...
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bioRxiv - Microbiology 2020Quote: ... All cells used in this study were cultured in a humidified incubator at 37°C with 5% CO2 and routinely tested and certified as mycoplasma-free using the MycoAlert kit (Lonza). STR analysis (Idexx ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were confirmed to be mycoplasma-free by MycoAlert Mycoplasma Detection Kit (Lonza, Morristown, NJ USA; Cat #: LT07-118). Cells were steroid-starved in phenol red-free medium containing 10% charcoal stripped-FBS (5% CS-FBS for C4-2 cells ...
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bioRxiv - Cell Biology 2021Quote: ... IPSCs were cultured in mTeSR1 and differentiated using Wnt modulation.(Lian et al., 2012) IPSCs were verified free of mycoplasma using MycoAlertTM (Lonza). iPSC-CM were purified by either SIRPA-selection using magnetic beads at day 15 (Herron et al. ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were regularly tested to check they were free of mycoplasma contamination using a commercially available system (MycoAlert Mycoplasma Detection kit; Lonza). HCV replication experiments were conducted using the highly permissive human hepatoma cell line Huh7 High Passage (28 ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were rapidly transferred to glass Dounce homogenizers and homogenized in 750µL of endotoxin free water (Lonza W50-100). Homogenized samples were then diluted 1:5 in endotoxin free water in pyrogen-free glass tubes (Lonza N207 ...
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bioRxiv - Bioengineering 2022Quote: ... All cells were routinely verified as free of mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Group, Basel, Switzerland). MDA-MB-231 cells were first transduced with lentivirus (MOI=5 ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed once and cultured in infection medium consisting of either serum free EMEM supplemented with 100 IU/ml penicillin-100 μg/ml streptomycin mixture (Lonza), 2 mM L-glutamine (Lonza) ...