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Citations for Lonza :
401 - 450 of 665 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Astrocytes were transfected with Lonza glial transfection solution and electroporated with a Nucleofector machine (Lonza). Cells were then plated on appropriate supports previously coated with poly-L-Ornithine (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... HK-2 cells were nucleofected using Mirus nucleofection solution and T20 program of nucleofector (Lonza).
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bioRxiv - Microbiology 2020Quote: ... pH 7.3) or P3 solution from the P3 Primary Cell 4D-Nucleofector X Kit (Lonza). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 million hESCs were resuspended with 100uL Lonza Amaxa P3 nucleofection solution (Lonza V4XP-3024), the annealed ...
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bioRxiv - Immunology 2022Quote: ... centrifuged at 400xg for 5 min and resuspended in Hank’s Balanced Salt Solution (HBSS, Lonza). Paraformaldehyde (PFA ...
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bioRxiv - Zoology 2022Quote: ... EO-114 (specific for mammalian fibroblast recommended by Lonza and compatible with P2 solution kit), FF-135 (specific for human fibroblast and dental palp cells [28-30] ...
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bioRxiv - Genomics 2024Quote: ... We used the Lonza P3 Primary Cell Nucleofector Solution X Kit L (Lonza, VXP-3024) for all nucleofection experiments ...
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bioRxiv - Microbiology 2024Quote: ... The DNA constructs in TE buffer were mixed with 90µl P3 Primary cell solution (Lonza) and used to resuspend 20µl segmented schizonts which were subsequently transferred to a transfection cuvette ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection was carried out in P3 Primary Cell 4D-Nucleofector™ Solution (Lonza, V4XP-3032) and program E0-115 was used ...
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bioRxiv - Neuroscience 2024Quote: ... 50’000 preMacs per reaction were resuspended in 20 µl P3 Primary Cell Nucleofector Solution (Lonza) and mixed with CRISPR/Cas9 RNPs before nucleofection with CM-137 pulse code in a 96-well nucleofection plate using a 4D-Nucleofector 96-well Shuttle nucleofector (Lonza) ...
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bioRxiv - Developmental Biology 2024Quote: FSPS13b hiPS cells were nucleofected using the P3 Primary Cell NucleofectorTM Solution (Lonza #V4XP-3024) with pulse code CA137 ...
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bioRxiv - Cell Biology 2023Quote: Human ESCs were nucleofected using an Amaxa nucleofector II in Nucleofector Solution L (all Lonza) using B-016 preset ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 20 µL of P3 Primary Cell Nucleofector solution (Lonza #V4SP-3096) and mixed gently with 4 µL of NTC complexes ...
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bioRxiv - Genomics 2023Quote: ... The cells were washed once with PBS and resuspended in P3 Primary Cell solution (Lonza). RNP complexes were formed by mixing Cas9 protein (IDT ...
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bioRxiv - Biochemistry 2024Quote: ... OPCs were gently resuspended in P3 Primary Cell Nucleofector solution (Lonza Cat. No. V4XP-3032) at 10000 – 12500 cells/µl ...
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bioRxiv - Genetics 2024Quote: ... We used nucleofection (4D-Nucleofector X Unit and P3 4D-Nucleofector™ X Solution, Lonza) to transfect both the AAVS1 targeting plasmids described above and transcription activator-like effector nucleases (hAAVS1 TALEN Left and Right were gifts from Su-Chun Zhang ...
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bioRxiv - Cancer Biology 2024Quote: ... 1081059) in RNase-free PBS in 12μl Primary Cell P3 Nucleofector solution (Lonza, V4XP-3032) was used to generate control cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 1081059) in RNase-free PBS in 12μl Primary Cell P3 Nucleofector solution (Lonza, V4XP- 3032) and incubating at RT for 20 min ...
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bioRxiv - Cell Biology 2024Quote: hiPSCs (8 × 105 cells) were electroporated in P3 primary cell nucleofector solution (Lonza, V4XP-3032) with 2 µg of FAM53C KO Plasmid containing GFP expression system (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 x 105 cells were suspended in 100 µl of P3 Nucleofector™ solution (Lonza) per reaction ...
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bioRxiv - Genetics 2022Quote: CRISPR plasmid constructs (2 μg) were electroporated into 2 × 106 Raji cells using the Amaxa Cell Line Nucleofector Kit V (Lonza Bioscience) (Program M-013) ...
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bioRxiv - Neuroscience 2021Quote: ... was pressure-injected from a single barrel glass micropipette into the brain tissue or dilute agarose gel (0.3% w/v in PBS; NuSieve GTG agarose, Lonza, Cat. # 50081) and a sequence of images were taken using a charged-coupled device (CCD ...
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bioRxiv - Plant Biology 2021Quote: ... pollen was collected immediately prior to bombardment and distributed on pollen germination medium solidified with 1% (w/v) NuSieve GTG Agarose (Lonza, Switzerland). Pollen germination media for Nicotiana (Wang and Jiang 2011 ...
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bioRxiv - Biophysics 2020Quote: ... or in Binding buffer (1% w/v BSA and 25 mM HEPES pH 7.2 in DMEM without phenol red; Lonza Netherlands B.V.) at 4°C in the case of HeLa cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Transient transfection of pp150 in HFF was performed by transfecting pp150 expressing plasmid (kindly provided by Edward Mocarski) using Amaxa Nucleofection kit V (Lonza inc.). The pp71-expressing plasmid (pCGN-pp71 ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to a density of 1×106 cells and electroporated with 5μg of plasmid DNA (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Cells from each cell line were cultured for an additional 48 hours until total RNA isolation (Maxwell RSC simplyRNA kit ...
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bioRxiv - Immunology 2020Quote: ... and brachial lymph nodes were collected and then embedded in 6 % w/v low melting point agarose (Lonza, Walkersville MD, USA) in 1X PBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-Fc stable clone was obtained by electroporation with 2×106 cells and 5 μg endotoxin-free plasmids using Amaxa kit V and program U24 with Amaxa Nucleofector 2B (Lonza, Switzerland). Electroporated cells were subsequently plated in 96-wells at 500 cells/well in Plating Medium containing 80% EX CELL® CHO Cloning Medium (Cat.no C6366 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Then the supernatant was removed and cells were resuspended in 100 µl room-temperature Nucleofector® Kit V (Lonza, VCA-1003) solution ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug solutions were removed and wells washed twice with 1 x phosphate buffered saline (PBS; Lonza). Fresh Complete
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector SF, program FF-120), with 3000 ng LSR plasmid and 2000 ng pseudosite attD plasmid ...
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bioRxiv - Developmental Biology 2020Quote: ... The cell suspensions were mixed with ultra low melting agarose solution (3% SeaPrep®, LONZA, #50302) in a volume ratio of 1:1 and were loaded onto the two aqueous phase inlets of the FFD ...
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bioRxiv - Molecular Biology 2022Quote: ... washed with PBS and resuspended in 15 μL of SF Cell-Line Solution (V4XC-2032, Lonza) or P3 Primary Cell Solution (V4XP-3032 ...
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bioRxiv - Immunology 2022Quote: NK cells were centrifuged and resuspended in P3 Primary Cell Nucleofector© Solution (Lonza, V4XP-3032) at 3.33×108 cells/mL ...
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bioRxiv - Bioengineering 2020Quote: ... neurons were initially incubated with a plating medium containing 86.55% Minimum Essential Medium Eagle’s with Earle’s Balanced Salt Solution (MEM Eagle’s with Earle’s BSS, Lonza), 10% Fetal Bovine Serum (re-filtered ...
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bioRxiv - Cell Biology 2020Quote: ... cells were resuspended in 100 μl Human MSC Nucleofector Solution (Human MSC Nucleofector® Kit - Lonza) and the transfection was carried out according to the manufacturer’s instructions using different amount of gRNA plasmids or pmaxGFP plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted cells were resuspended in 100 μL of complete P3 Primary Cell Solution (Lonza #V4XP-3024), then mixed with the RNP/ssODN by trituration ...
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bioRxiv - Immunology 2020Quote: ... the cells were resuspended in 20 µL nucleofection solution P1 or P3 (with supplement added, Lonza) per 4 · 105 cells and 5 µL pre-formed RNP or nucleofector solution (no RNP control ...
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bioRxiv - Immunology 2020Quote: ... the cells were resuspended in 5 µL nucleofection solution P1 or P3 (with supplement added, Lonza) per 4 · 105 cells ...
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bioRxiv - Cell Biology 2021Quote: ... The basal compartment during treatment was filled with 1000μL HBSS (Hanks balanced salt solution Lonza, Switzerland) to omit influences of the complex and proprietary cell culture medium on later analyses ...
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bioRxiv - Microbiology 2022Quote: ... Pelleted cells were resuspended in SF Cell Line Nucleofector Solution with added supplement (Lonza catalog # V4XC2024). 25 µg of the plasmid to be transfected (Aldevron ...
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bioRxiv - Bioengineering 2024Quote: ... 1x phosphate buffered saline (PBS) solution without calcium or magnesium was obtained from Lonza (MD, USA). HLFs were cultured in treated tissue culture flasks with fibroblast media supplemented with 2% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... 5 million cells were suspended in 74 μl of P4 Primary Cell solution (Lonza, VSOP-4096). In parallel ...
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bioRxiv - Cell Biology 2023Quote: ... Macroscopically healthy tissue was immediately washed with sterile Hank’s balanced salt solution (HBSS; Lonza, Walkersville, MD), and portions were ...
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bioRxiv - Immunology 2023Quote: ... suspended in a concentration of 1.5×106 in 100 µl cell line nucleofector solution T (Lonza) and nucleofected with 5 μg of a plasmid using program X-001 in the Nucleofector IIb device (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 x 106 hiPSCs were resuspended in 100 µl P3 solution with supplement (Lonza, #V4XP-3024) and transfected with 2.5 µg linearized RUNX1 HDR template and 4 µg each of pCW-Cas9 plasmid (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... One million cells resuspended in 100 μL of SF Cell Line Nucleofector solution (Lonza V4XC-2012) were electroporated with 2 μg plasmid using pulse code FF-120 (Lonza Amaxa 4D Nucleofector) ...
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bioRxiv - Biophysics 2021Quote: ... for all imaging experiments involving exogenous expression we used the Lonza Amaxa II Nucleofector System with Cell Line Nucleofector Kit V reagent (Lonza VCA-1003). Briefly ...
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bioRxiv - Cell Biology 2020Quote: 1 million Jurkat cells were electroporated with the 2.5 μg of sgRNA plasmid and GFP control plasmid at a 10:1 ratio using an Amaxa Cell Line Nucleofector Kit V and an Amaxa™ Nucleofector™ II (Lonza). HeLa ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs were transfected into 3T3-F442A preadipocytes or OP9 preadipocytes via electroporation using the Amaxa Cell Line Nucleofector Kit V (Lonza, Cologne, Germany). Afterwards cells were grown two days in DMEM ...