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Citations for Lonza :
451 - 500 of 665 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 4×106 cells were electroporated with 10 μg DNA of pBluescript II SK(+) carrying a human U6 promoter and EGFP-sgRNA using Nucleofector® V Kit (Lonza, Switzerland) as described above.
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells (grown in 6-well dishes) were subjected to nucleofection with Amaxa® Cell Line Nucleofector® kit V (VCA-1003, Lonza), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 bulk or stable populations overexpressing Nluc-CD63 or Nluc-CD9 constructs were obtained using an electroporation-based transfection protocol optimized for this cell line (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... We transfected the KCNQ1 constructs and the empty vector into our established mouse pancreatic β cells (referred to as SJ β cells (Jia et al., 2015)) using AmaxaTM Cell Line NucleofectorTM Kit V and AmaxaTM NucleofectorTM 2b device (Lonza, Switzerland). The glucose-sensitive mouse β-cell line SJ-β was cultured and an insulin-secretion assay performed as reported earlier (Jia et al. ...
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Biophysics 2022Quote: ... 106 U2OS cells were transfected with 1 μg of px330 plasmid and 1 μg of the HDR donor plasmid using the Nucleofector 2b device and cell line nucleofector kit V (Lonza, VCA-1003) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 1uM siRNA was electroporated into cells using the Amaxa Nucleofector II device and Cell Line Nucleofector Kit V (Lonza Bioscience, VCA-1003). B16-F10 cells were electroporated with program P-020 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A high resistance Plastiape® RS00 inhaler (Plastiape S.p.A, Osnago, Italy) containing size #3 HPMC capsules (V-Caps® Plus, Lonza, Morristown, NJ) and 1-3 mg of powder was attached to a USP induction port by a molded silicon adapter ...
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bioRxiv - Molecular Biology 2020Quote: pSBbi-Pur-ABCC1 or empty vector were cotransfected with SB100X using the same Amaxa Nucleofector II and kit V (Lonza Group AG). Stably expressing cells were selected for using 10ug/mL puromycin (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with pcDNA3.1 empty vector or pcDNA3.1 containing the human coding sequence of the VGLL2-NCOA2 fusion using the AMAXA cell line Nucleofection kit V (Lonza #VCA-1003;) and were selected in growth media containing 1μg/ml concentration of G418 (ThermoFisher #10131035) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were co-transfected with 1 ug of firefly Luciferase vector and 0.1 ug Renilla luciferase vector by nucleofection with Lonza Cell Line Nucleofector® Kit V (Lonza, #VVCA-1003) and the T-020 program in the Amaxa Nucleofector II device ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected into 106 U2OS cells using the Amaxa Nucleofector System with the Cell Line Nucleofector Kit V (catalog no: VCA-1003, Lonza, Cologne, Germany) utilizing program X-001 according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: CHO-S cells were stably transfected by electroporation using the Amaxa Nucleofector system in combination with the Cell Line Nucleofector® Kit V (Lonza, Switzerland). 5.0 x 106 cells per cuvette were transfected with 2 µg plasmid and incubated in T75 flasks at 37 °C under 5% CO2 in 13 ml CD-CHO medium supplemented with 8 mM L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: RAW 264.7 cells and MDA-MB-231 cells were transitly transfected by nucleofection in an Amaxa Nucleofector II device using Amaxa Cell line kit V (Lonza, VACA-1003) following a pre-existing protocol [101] ...
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bioRxiv - Bioengineering 2021Quote: Landing pad cell lines were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with the promoterless mCherry donor containing the matching attP at a dose of either 1000 ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 WT K562 were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Bioengineering 2021Quote: 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of a pEF-1α-PuroR-P2A-mCherry donor plasmid containing an upstream Cp36 attD site (pJT371) ...
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bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of the same Cp36 PuroR-P2A-mCherry donor ...
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bioRxiv - Genetics 2020Quote: ... Parasites were subsequently resuspended in 100 μL nucleofector solution of the Basic Parasite Nucleofector Kit 2 (Lonza), combined with 50 μL plasmid solution containing 5-10 μg DNA ...
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bioRxiv - Immunology 2021Quote: ... 1-10 million activated P14 cells were re-suspended in 20 μL primary cell nucleofection solution (Lonza), then mixed and incubated with the crRNA/Cas9 mix for 2 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Ipsilateral and contralateral brain tissues were placed in cold Hank balanced salt solution (HBSS + Ca/Mg; Lonza) and mechanically dissected through a 100 µm cell strainer ...
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bioRxiv - Biophysics 2021Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
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bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μl Amaxa solution (Lonza Nucleofector 2b, program T-016), with 300 ng of the 11.6 kb LSR plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... The final solution (approximately 125 µL) was gently mixed and transferred to a 100 µL cuvette (Lonza). Electroporation was then performed according to the manufacturer’s instructions on the 4D-Nucleofector™ Core Unit (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... following the protocol of 4D-Nucleofector™ Solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza). In this setup ...
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bioRxiv - Neuroscience 2023Quote: ... Cell- plasmid solution was transferred to the electroporation cuvette (AmaxaTM P3 primary cell 4D- NucleofectorTM X, Lonza) and electroporated using the program CB-150 of the Nucleofector ...
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bioRxiv - Molecular Biology 2022Quote: ... and re-suspended in 20 μL NF-solution (P3 Primary Cell 4D-Nucleofector™ X Kit, Lonza). Thereafter ...
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bioRxiv - Genomics 2022Quote: ... One million sorted Pro-E cells were resuspended with 100 μl solution P3 for primary cells (Lonza); preassembled RNP were then added to the cell suspension for nucleofection ...
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bioRxiv - Systems Biology 2024Quote: ... cultured cells were washed by dispensing and aspirating 37°C HEPES buffered saline solution (Lonza, CC-5022) and then dissociated with 0.025% Trypsin/EDTA (Lonza ...
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bioRxiv - Immunology 2024Quote: ... cells were washed once with PBS and resuspended in T cell nucleofection solution (Lonza, Cat: VPA-1002) containing plasmids of choice and nucleofected using the T-023 program on the Amaxa IIb nucleofector ...
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bioRxiv - Biophysics 2023Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
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bioRxiv - Molecular Biology 2023Quote: One million Aire wild-type mTEC 3.10 cells were suspended in 100 μL of nucleofector solution (Lonza) containing 20 pmol of spCas9-GFP ...
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bioRxiv - Immunology 2022Quote: ... approximately 5 million cells were suspended in 74 μl of P4 Primary Cell solution (Lonza, VSOP-4096). In parallel ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... The supernatant solution was collected and the DNA library was confirmed by Flashgel (Lonza, catalog no. 57023), and then purified using a Zymo DNA Clean& Concentrator™-5 kit (Zymo Research ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 μg plasmid DNA in a volume of 25-30 μL of AD1 Solution (Lonza Bioscience) was put as a drop on the slice ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffered saline solution (CC-5024, Lonza) before renewing the medium or passaging the cells ...
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bioRxiv - Microbiology 2022Quote: ... mixed with DNA and/or RNA as indicated in each experiment and electroporated at 110 V for 10 ms by the electroporator (Lonza Amaxa Nucleofector 2B). After electroporation ...
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bioRxiv - Immunology 2024Quote: ... For transient transfection 1e6 Jurkat cells were transfected with 2 μg DNA using the LONZA electroporator (program X-001) and Nucleofector Kit V (Lonza, catalog no. VCA-1003) 24 h prior to imaging ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cell/DNA suspension was transferred into the certified Nucleofector® cuvette and the V-001 program of Nucleofector® 2b Device (Lonza, AAB-1001) was applied ...
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bioRxiv - Immunology 2024Quote: RAW264.7 Mφs (2 x 106, ATCC, Cat# ATCC TIB-71) were electroporated (Amaxa® Cell Line Nucleofector® Kit V, LONZA; Cat#VCA-1003) with 5 µg of HA-HIF1alpha (Addgene#18949 ...
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bioRxiv - Immunology 2024Quote: RAW264.7 Mφs (2 x 106, ATCC, Cat# ATCC TIB-71) were electroporated (Amaxa® Cell Line Nucleofector® Kit V, LONZA; Cat#VCA-1003) with 0.5 µM of scrambled (ThermoFisher ...
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bioRxiv - Pathology 2024Quote: ... Series of transfections in the murine hybridoma/myeloma cell line SP2/0 (ATCC® CRL-1581™) were performed using Cell line Nucleofactor kit V (Amaxa/Lonza, Basel, Switzerland) and a Nucleofactor II device (Amaxa/Lonza ...
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bioRxiv - Bioengineering 2024Quote: ... and 800,000 cells were nucleofected with 2.5 µg of Super piggyBac Transposase (SBI cat. no. PB200A-1) and 10 µg of transposon plasmid using Lonza Cell Line Nucleofector Kit V (Lonza cat. no. VCA-1003) on an Amaxa IIb Nucleofector with program B-016 ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5X105 cell were resuspended in 20µL nucleofection solution and nucleofected (4D-Nucleofector TM Core + X Unit [Lonza] ...
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bioRxiv - Neuroscience 2022Quote: ... 5 × 106 trypsinized cells were resuspended in 93.5 µl solution (mouse ES cell nucleofector kit, VPH-1001, Lonza). The solution includes 2 µg of each CRISPR/Cas9 vector (4 µg total ...
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bioRxiv - Microbiology 2020Quote: ... 1 million Jurkat T-cells were resuspended in 100 μL Nucleofector solution (Cell Line Nucleofector™ Kits, Lonza) and combined with 2 μg of the linearized dual-fluorophore vector and 2 μg of the corresponding gRNA/Cas9 plasmid ...
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bioRxiv - Genomics 2021Quote: ... 0.3×106 cells were washed with PBS and resuspended in 20 μL of SF Cell Line solution (Lonza). The cells were combined with the RNP mix and 0.6 μg pDNA donor and electroporated using program DJ-100 ...
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bioRxiv - Immunology 2021Quote: ... The virus was propagated in the human neuroblastoma cell line SK-N-SH in Eagle’s Minimum Essential Medium (EMEM) with Earle’s Balanced Salt Solution (EBSS) (Lonza), supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... 5 wt% polymer solutions were created by dissolving lyophilized GelMA in phosphate buffered saline (PBS; Lonza, 17-516F) at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... 0.3×106 cells were washed with PBS and resuspended in 20 μL of SF Cell Line solution (Lonza). The cells were combined with the RNP mix and electroporated using program DJ-100 ...