Labshake search
Citations for Lonza :
351 - 400 of 449 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... A total of 5×106 cells per transgene were washed with phosphate-buffered saline (PBS) and resuspended in 100µl NucleofectorTM Solution V (Lonza, Germany) containing the expression vector (Fig ...
-
bioRxiv - Cancer Biology 2024Quote: ... Electroporation was performed with the Lonza SF Cell line 4D-Nucleofector X solution (#V4XC-2032) and the 4D-Nucleofector X Unit (Lonza) (#AAF-1003X ...
-
bioRxiv - Neuroscience 2024Quote: ... 5 x 105 to 1 x 106 cells were resuspended in 20µL of nucleofection solution (P3 Primary Cell 4D-Nucleofector™; Lonza) and nucleofected (Lonza 4D nucleofector TM Core + X Unit ...
-
bioRxiv - Neuroscience 2023Quote: The modified pC13 dCas9-BFP-ZIM3 construct with CLYBL homology arms was co-nucleofected into 125Q iPSC with two additional constructs carrying TALENs targeting the CLYBL locus (pZT-C13-R1 and pZT-C13-L1, addgene #62196, #62197 (Cerbini et al., 2015)) in P3 solution using program CA137 in a 4D-Nucleofector (Lonza). Cells were seeded at clonal density and selection in G418 was initiated 48 hours later ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were pelleted and resuspended in 100 μl of P3 transfection solution (82 μl Amaxa Buffer and 18 μl P3 supplement; Lonza) and 10 μl of DNA mix ...
-
bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
-
bioRxiv - Immunology 2024Quote: ... siRNA against human genes or control siRNA were incubated with a mixture of nucleofection solution (Human Monocytes Nucleofector kit; Lonza) and primary human monocytes and placed in nucleofection cuvettes subjected to program Y-010 for the Nucleofector 2b Device (Lonza) ...
-
bioRxiv - Biochemistry 2022Quote: ... washed once with phosphate-buffered saline (PBS) and resuspended at 107 cells/mL in P4 Primary Cell Nucleofector Solution (Lonza). 20 µL of resuspended cells (corresponding to ~ 200,000 cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 pmol of the dsODN was mixed together with 100 µl homemade nucleofection solution to the RNP complex and electroporated using Nucleofector 2b (Lonza) with A23 program and 2 mm electroporation cuvettes.
-
bioRxiv - Immunology 2022Quote: ... 1×106 cells were electroporated (pulse code EH100) with Cas9 ribonucleoprotein and DNA templates in 20 ul Nucleofector Solution P3 (Lonza) with a 4D Nucleofector X unit (Lonza) ...
-
bioRxiv - Genomics 2023Quote: ... purified Percoll-enriched mature schizonts (∼20 μl packed volume) were suspended in 100 μl of P3 primary cell solution (Lonza) containing 60 μg of linearized repair plasmid DNA (repair plasmid 1 and 2 separately in two separate transfection experiments ...
-
bioRxiv - Synthetic Biology 2023Quote: ... An array of 20µL droplets of cell-laden hydrogel solutions were pipetted between two 25x75mm glass slides that were thrice treated with GelSlick (Lonza) separated by 3D-printed 400μm insert ...
-
bioRxiv - Neuroscience 2023Quote: ... and the ssODN (1 μl; stock 100 μM, IDT) in 20 μl of nucleofection buffer P3 (P3 Primary Cell NucleofectorTM Solution, Lonza) were nucleofected (program CA137 ...
-
bioRxiv - Immunology 2024Quote: ... They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the V-001 program (Amaxa Biosystems) ...
-
bioRxiv - Biophysics 2024Quote: ... 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).
-
bioRxiv - Biophysics 2024Quote: ... 0.5 μg of F-tractin-mScarlet plasmid and 0.75 ug of Zyxin-mNeonGreen plasmid were mixed with solution SE from SE Cell Line 4D-Nucleofector S Kits (Lonza). 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).
-
bioRxiv - Immunology 2024Quote: ... and resuspended at 2E4-7.5E4 cells/µL in freshly supplemented P3 Primary Cell Nucleofector Solution (Lonza, catalog no. V4SP-3096). For single RNP edits ...
-
bioRxiv - Immunology 2024Quote: ... 1 million CD4+ T cells were re-suspended in 20 µl primary cell nucleofection solution (P2 Primary Cell 96-well Nucleofector kit, Lonza) and mixed with 8 µl precomplexed Cas9/RNP ...
-
bioRxiv - Immunology 2024Quote: ... 3µL of gRNA was mixed with 1µL electroporation enhancer (IDT 1075915) and 20µL of Jurkat T cells (1 x 105) in nucleofection solution (Lonza V4XC-1032). The mixture was transferred to a 16-well Nucleocuvette Strip and electroporated with the 4D-Nucleofector (Lonza ...
-
bioRxiv - Microbiology 2024Quote: ... 1 μl of isolated schizonts was mixed with 7 μl of DNA and 18 μl of P3 Primary Cell 4D-Nucleofector solution from Lonza. This mixture was then added to a well within a 4D-Nucleofector 16-well strip from Lonza and electroporated using the FI-115 program on the Amaxa Nucleofector 4D ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cells were first electroporated with a PiggyBac-configured plasmid containing the Dox-inducible NbALFA-ABEL degrader cassette and a plasmid encoding the PiggyBac transposase at a 3:1 mass ratio via nucleofection (solution E with program CM138) according to manufacturer’s instructions (Lonza Inc.), followed by puromycin selection (5μg/ml ...
-
bioRxiv - Bioengineering 2024Quote: 3·106 BRPKp110 cells were resuspended in 20 µl primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X kit S (32 RCT, V4XP-4032; Lonza). Cells were mixed and incubated with 15 µl RNP at room temperature for 2 minutes ...
-
bioRxiv - Genetics 2024Quote: 61 pmol Recombinant Alt-RspCas9 protein (IDT) in 10 µL of Human Keratinocyte Nucleofector™ Kit solution (Lonza, VPD-1002) for 10 min and immediately used for nucleofection of 8×105 primary keratinocytes with the Amaxa nucleofection apparatus (Lonza ...
-
bioRxiv - Bioengineering 2024Quote: ... and resuspended at a concentration of 2.5 x 105 cells in 20 μl of pre-mixed P3 Primary Cell 4D-Nucleofector solution (P3 Primary Cell 4D-Nucleofector X Kit S, Cat: V4XP-3032; Lonza). Cells were combined with the RNP/HDR plasmid solution and transferred into individual wells of a 16-well Nucelocuvette Strip (Lonza) ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were combined with the RNP/HDR plasmid solution and transferred into individual wells of a 16-well Nucelocuvette Strip (Lonza). Nucleofection was performed using 4D-Nucleofector Core (Cat ...
-
bioRxiv - Biochemistry 2024Quote: ... and resuspended in 20 µL of supplemented SE Nucleofector Solution as per the manufacturer’s guidelines for HeLa and HCT116 cells (Lonza Bioscience). The entire RNP complex solution and 1 µL 100 µM Alt-R Cas9 electroporation enhancer (IDT ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.3×106 live cells were resuspended with the RNP complex and 20 µL of P3 Primary Cell Nucleofector Solution (Lonza), following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 × 106 cells were centrifuged at 90 x g for 10 minutes and then resuspended in 100 μl nucleofector solution V (Lonza). The cells were combined with 2000 ng plasmid DNA and placed in a specially designed cuvette ...
-
bioRxiv - Bioengineering 2021Quote: ... we nucleofected HEK293FT-RfxCas13d cells with 225 μmol of synthetic crRNAs in 20μl reaction of SF Cell Line Nucleofector Solution (Lonza V4XC-2032) using the Lonza Nucleofector 4D (program CM-130) ...
-
bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cell suspension with trypsin-EDTA (Pan-Biotech, Germany) for 3 min followed by trypsin neutralizing solution (Lonza, Germany). Single cell suspensions of secondary mammospheres were obtained as described for day 7-first generation mammospheres.
-
bioRxiv - Neuroscience 2022Quote: We prepared thin agar pad for the samples where 8 µL of semi-cooled 1.5% agarose solution (add low melting temperature NuSieve GTG Agarose (Lonza, Cat# 50081) to 2% galactose media (0.77 g of CSM-URA (Sunrise # 1004-010) ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended with 10µL of primary cell nucleofection solution (P3 primary Cell 4D-Nucleofector X kit S (Lonza, # V4XP-3032), and mixed with RNP complexes ...
-
bioRxiv - Microbiology 2022Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and to a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC5002); this was repeated to maximize cell collection ...
-
bioRxiv - Developmental Biology 2020Quote: ... or a PMO Control at 100 µM in 100 µL solution from the P3 Primary Cell 4D-Nucleofector® X Kit L (V4XP-3024, Lonza) using the CB150 program on the 4D-Nucleofector™ System (Lonza) ...
-
bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
-
bioRxiv - Genomics 2023Quote: ... and 120 pmol of in vitro synthesized gRNA were electroporated into 250,000 MCF7 cells with the P3 primary nucleofection solution (Lonza, V4XP-3024), using the DN-100 Lonza 4D-Nucleofector program ...
-
bioRxiv - Genetics 2023Quote: All iPSC lines were passaged as clumps or single cells upon reaching 80-85% confluency using Versene (0.02% EDTA solution, Lonza #17-711E) or StemPro Accutase (Gibco #A1110501) ...
-
bioRxiv - Molecular Biology 2022Quote: ... collected by centrifugation at 500xg for 5 min and resuspended in SF solution (SF cell line 4D-Nucleofector™ X Kit, Lonza). After addition of the RNP ...
-
bioRxiv - Biophysics 2023Quote: ... 1.5mL for a T75 flask for 5 minutes at 37° C and resuspended in 80 μL of SF cell line solution (Lonza; Basel) per flask ...
-
bioRxiv - Genomics 2022Quote: ... We used 1.0 × 106 HCT116 or U2OS cells to transfect the base editor plasmids (2600 ng) using SE solution and 4D-nucleofector (Lonza, Switzerland) as per the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Sections attached to slides were fixed with cold acetone/ethanol (1:1) solution and washed in PBS buffer (Lonza, Morristown, NJ). Primary antibodies and isotype controls (Supplementary Table 9 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 20 µL of cell suspension (200,000 cells) was mixed with the RNP-containing solution and electroporated using the 4D-Nucleofector X Unit (Lonza, Basel, Switzerland) with the CM138 nucleofection program ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 4 million cells (48 h knockdown ...
-
bioRxiv - Immunology 2024Quote: ... The DPP4 KO and NTC groups were washed with 1X PBS and resuspended with P3 primary nucleofection solution (Lonza, cat. V4XP3012) containing ribonucleoprotein (RNP ...
-
bioRxiv - Biochemistry 2024Quote: ... 5.0 × 106 OSCs were mixed with 200 pmol of siRNAs in 20 μL of Solution SF of the Cell Line Nucleofector Kit SF (Lonza Bioscience) and electroporation was performed using a Nucleofector 96-well Shuttle device (Lonza Bioscience) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza) ...
-
bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
-
bioRxiv - Microbiology 2022Quote: ... Cells that were harvested at 48 hours were first washed by dispensing and aspirating 37°C HEPES buffered saline solution (Lonza, CC-5022), and then trypsinized with 0.025% Trypsin/EDTA (Lonza ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Biochrom AG, Berlin, Germany) and 1% (v/v) antibiotic–antimycotic solution (AAS, Lonza, Basel, Switzerland) was used for culturing human dermal fibroblasts (HDF ...
-
bioRxiv - Immunology 2020Quote: ... 50 pmol sgRNA and 20 pmol Cas9 were combined and diluted to 20 µL with nucleofection solution P1 or P3 (with supplement added, Lonza, Basel, Switzerland). The mixture was incubated at 25 °C for 10 min or up to 2 h and immediately used to electroporate moDCs ...