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Citations for Lonza :
301 - 350 of 449 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use in functional assays.
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bioRxiv - Cancer Biology 2023Quote: ... 3uL of the total Cas9-RNP complex was added to 200,000 K562 cells resuspended in 18uL SF cell solution (Lonza) and subjected to nucleofection with the Lonza 4D nucleofectorsystem using manufactured specified conditions for K562 cells ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted at 1000 rpm for 5 min and resuspended in 100 μL nucleofector solution (Lonza, #VPB-1002) before adding 2.5 μg of plasmid ...
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bioRxiv - Physiology 2023Quote: Purified HA was mixed with sucrose solution (final concentration of 333 mM) and loaded to a 0.4% SeaKem Gold agarose gel (Lonza). HA-Ladders (Hyalose ...
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bioRxiv - Genomics 2023Quote: ... Cells were grown at 37°C and 5% CO2 and passed with Hepes buffered saline solution (Lonza, CC-5024) and 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and resuspended at 7.5E4 cells/µL in freshly supplemented P3 Primary Cell Nucleofector Solution (Lonza, catalog no. V4SP-3096). 20 µL of cell were mixed with 7 µL RNP ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use.
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bioRxiv - Biophysics 2024Quote: Gel slides for bacterial spatial fixation were prepared as follows: A solution of 1.5% (w/v) low melting agarose (Lonza) in PBS was heated for approximately 20 seconds to allow full melting of the agarose ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellet was resuspended in 20 µL of pre-warmed P3 primary cell solution (Lonza Kit, V4XP-3032), 2.5 µL RNP complex ...
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bioRxiv - Developmental Biology 2024Quote: ... larvae were anesthetized using 0.0053% tricaine in a PTU/E3 solution and then mounted on a glass microscopy slide using 2.5% low-melt agarose (SeaPlaque, Lonza) in a PTU/E3 solution containing 0.016% tricaine ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 clonal Kp03 landing pad cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended and transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution in a 25 µL electroporation cuvette (Lonza). Electroporation of GCaMP mutants was performed according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed with 1X phosphate buffered saline (PBS) and resuspended in 20 µL of solution SF or SE (Lonza). Cell suspensions were combined with RNP complex(es) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Genomics 2020Quote: ... 3 μL RNP complex solution was mixed with the cells and the cells were nucleofected using program DJ-110 on a 4D-Nucleofector (Lonza) with 16-well strips ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution electroporation cuvettes (Lonza). Electroporation was performed according to the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... between 1 x 106 and 1 x 107 cells were resuspended in 18 μl of P3 primary cells solution (Lonza), 2 μl of the restricted cassette (2-4 μg total ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μg of linearized plasmid containing wild-type or Ku70 3A cDNA was transfected using Amaxa nucleofector solution V (Lonza) and program T-020 ...
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bioRxiv - Cancer Biology 2021Quote: ... percent cell death was quantified by flow cytometry or cells were re-suspended in media and an equal volume of Trypan blue solution (Lonza) was added ...
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bioRxiv - Microbiology 2021Quote: 2×106 Elijah cells were washed in PBS and resuspended in 20 μl P3 Primary Cell Nucleofector Solution buffer prepared with Supplement 1 buffer (Lonza) according to the manufacturer’s instructions (P3 Primary Cell 4D-Nucleofector X Kit S) ...
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bioRxiv - Microbiology 2020Quote: ... 2019): The plasmid was linearized using PsiI-V2 and purified using phenol-chloroform precipitation and resuspended in P3 solution (Lonza). Successful linearization was confirmed using agarose-gel electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... 2×105 143B or HEK293T cells were resuspended in the resulting solution containing ribonucleoprotein complexes (RNPs) and electroporated using a 4D-Nucleofector (Amaxa, Lonza) programs FP-133 (143B ...
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bioRxiv - Immunology 2020Quote: ... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution in a 25-μL electroporation cuvette (Lonza). Cells were electroporated according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... 8 volumes of collagen I gel solution (10mg/ml) were mixed with 1 volume of 10X EMEM (Lonza: 12-684F) and 1 volume of 10X Reconstruction buffer(Anacker and Moody 2012 ...
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bioRxiv - Bioengineering 2020Quote: DNA delivery was carried out on 20 μL volume format using Amaxa 4D-Nucleofector System and Cell line nucleofector solution SF (Lonza). Total amount of DNA electroporated was 3 μg (1.5 μg each plasmid in the case of using two plasmids at the same time) ...
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bioRxiv - Developmental Biology 2020Quote: ... The guide RNA vector was then electroporated into the parent line containing the CRISPRi system using the Human Stem Cell Nucleofector Kit 1 solution with the Amaxa nucleofector 2b device (Lonza). Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM ...
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bioRxiv - Immunology 2020Quote: 2×106 freshly isolated primary B cells were washed in PBS and resuspended in 20 μl P3 Primary Cell Nucleofector Solution buffer prepared with Supplement 1 buffer (Lonza) according to the manufacturer’s instructions (P3 Primary Cell 4D-Nucleofector X Kit S) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg RNA was electroporated into BHK-21 cells using an Amaxa nucleofector 2b (program A-031) and Nucleofection T solution kit (Lonza). Transfected BHK-21 cells were mixed in a 1:1 ratio with cells susceptible to CoV infection ...
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bioRxiv - Immunology 2021Quote: ... 2.106 T cells were resuspended in 20 µl of nucleofection solution with 3 µl or 4 µl RNP and transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031) and Nucleofection T solution kit (Lonza). Transfected BHK-21 cells were mixed with HuH7 or Vero cells in a 1:1 ratio and plated for harvesting supernatants ...
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bioRxiv - Biochemistry 2022Quote: ... washed once with phosphate-buffered saline (PBS) and resuspended at 107 cells/mL in P4 Primary Cell Nucleofector Solution (Lonza). 20 µL of resuspended cells (corresponding to ~ 200,000 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 pmol of the dsODN was mixed together with 100 µl homemade nucleofection solution to the RNP complex and electroporated using Nucleofector 2b (Lonza) with A23 program and 2 mm electroporation cuvettes.
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bioRxiv - Immunology 2022Quote: ... 1×106 cells were electroporated (pulse code EH100) with Cas9 ribonucleoprotein and DNA templates in 20 ul Nucleofector Solution P3 (Lonza) with a 4D Nucleofector X unit (Lonza) ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Neuroscience 2024Quote: ... was slowly placed on top of the solution after cleaning the coverslip with RNAse AWAY (Molecular BioProducts) and 70% ethanol and coating the facing-down side of the coverslip with Gel Slick Solution (Lonza). The tissue was incubated at room temperature for 1.5 h to allow the gel to polymerize ...
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bioRxiv - Neuroscience 2024Quote: ... 5 x 105 to 1 x 106 cells were resuspended in 20µL of nucleofection solution (P3 Primary Cell 4D-Nucleofector™; Lonza) and nucleofected (Lonza 4D nucleofector TM Core + X Unit ...
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bioRxiv - Cancer Biology 2024Quote: ... Electroporation was performed with the Lonza SF Cell line 4D-Nucleofector X solution (#V4XC-2032) and the 4D-Nucleofector X Unit (Lonza) (#AAF-1003X ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × 106 wild-type K562 cells were electroporated in 100 μl of Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 µg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Supernatant was removed and cells were resuspended in P3 Primary Cell Nucleofector® Solution with Supplement 1 (Lonza, V4XP-3032) at 5-15 million cell/mL ...
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bioRxiv - Immunology 2024Quote: ... siRNA against human genes or control siRNA were incubated with a mixture of nucleofection solution (Human Monocytes Nucleofector kit; Lonza) and primary human monocytes and placed in nucleofection cuvettes subjected to program Y-010 for the Nucleofector 2b Device (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... 2005) using Amaxa Basic Parasite Nucleofector Solution 1 using the X-001 program of an Amaxa Nucleofector II (Lonza, Switzerland). Clones were selected by growth in medium containing phleomycin (2.5 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... Plasmids were sequenced at the OHSU sequencing core and transfected into BEAS-2B MR1-/- cells using the Amaxa nucleofection system with Kit T solution (Lonza). The transfection efficiency was evaluated via GFP expression by flow cytometry (Supplementary Figure 4) ...
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bioRxiv - Genomics 2024Quote: ... mRNA:gRNA were added directly to 1×106 cells resuspended in 20 µl of P3 solution and nucleofected with pre-programmed pulse EO-115 in 4D-NucleofectorTM System (Lonza). After nucleofection ...
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bioRxiv - Immunology 2024Quote: ... The reaction contained 400 pmol of Cas9 protein and 1 × 107 of the transduced NK cells in 100 µL of P3 solution (Lonza). Immediately after electroporation ...
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bioRxiv - Immunology 2024Quote: ... They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the V-001 program (Amaxa Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... 200,000 H1299 cells were resuspended in the resulting solution containing ribonucleoprotein complexes (RNPs) and electroporated using a 4D-Nucleofector (Amaxa, Lonza). Nucleofected cells were then expanded and single-cell cloned by limiting dilution by plating 0.5 cells/well in a 96 well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell and siRNA mixture resuspended in 100 µl of Nucleofector Solution for Human Keratinocytes provided with Human Keratinocyte Nucleofector kit (VPD-1002, Lonza). Nucleofection performed on program X-001 of Amaxa Biosystems Nucleofector II electroporator transfection unit ...