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Citations for Lonza :
3851 - 3900 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and maintained in D10 media or endothelial growth medium 2 (EGM-2) using the EGM-2 bullet kit from Lonza, respectively ...
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bioRxiv - Immunology 2022Quote: ... 2 million B cells were then washed with PBS and resuspended in 15 uL resuspension buffer (4D-Nuclcofector X Kit S, #V4XP-4032; Lonza). Cells in resuspension buffer (12.5ul ...
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bioRxiv - Physiology 2022Quote: ... All human iPSC lines were negative for mycoplasma contamination as tested using the Mycoalert Mycoplasma testing kits (LT07-318, Lonza) and no karyotype abnormalities were found (KaryoStat+ ...
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bioRxiv - Developmental Biology 2020Quote: ... Nucleofection was performed on an Amaxa nucleofector IIb using protocol O-003 with the mouse neuron kit (Lonza, VPG-1001). Cells were allowed to recover for 1 hour at 37 °C and followed by NCAM1 MACS and culture as described above.
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bioRxiv - Immunology 2021Quote: ... 2.106 T cells were resuspended in 20 µl of nucleofection solution with 3 µl or 4 µl RNP and transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Cell Biology 2019Quote: ... EGM-2 was prepared by combining components of an EGM-2 “bullet kit” (Cat no. CC-4176) and endothelial basal medium (EBM, Lonza, Walkersville ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 μg of plasmid DNA was introduced to 4×106 neurons using an AMAXA nucleofection kit (VPG-1001, VPG-1003; Lonza). AMAXA-nucleofected cells were plated in 35mm glass bottom imaging dishes ...
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bioRxiv - Genetics 2022Quote: ... The cells were treated with a dose range of high molecular weight poly(I:C) (1.5-8kb; 1mg/mL stock) by nucleofection following manufacturer’s instructions (Lonza 4D-NucleofectorTM Kits). Gene expression of the indicated genotypes was assessed by SYBR green based qPCR as described previously.
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bioRxiv - Cancer Biology 2022Quote: ... All cultures were tested every month for mycoplasma using the MycoAlert® Mycoplasma Detection Kit (Lonza, cat. no. LT07-318), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and a gene-edited iPSC homozygous MYBPC3 knock-out (MYBPC3 (-/-)) (Supplemental Table 1).(15–17) iPSCs were verified to be free of mycoplasma contamination using MycoAlert Detection Kit (Lonza). Newly generated lines were karyotyped (WiCell Institute ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines used in this study were routinely tested for mycoplasma and found to be mycoplasma-free (MycoAlert Mycoplasma Detection Kit MycoAlert, Lonza). Flp-In™ T-REx™ 293 cells were cultured according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines have been periodically subjected to re-confirmation by Short Tandem Repeat (STR) profiling by ATCC and mycoplasma testing by MycoAlertTM PLUS mycoplasma detection Kit (Lonza). YUHEF ...
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bioRxiv - Cell Biology 2024Quote: ... Those cells were cultured in DPSC growth medium (DPSC-GM) by adding the contents of a DPSC SingleQuots Kit (PT-4516; Lonza) to DPSC Basal Medium (DPSC-BM ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell and siRNA mixture resuspended in 100 µl of Nucleofector Solution for Human Keratinocytes provided with Human Keratinocyte Nucleofector kit (VPD-1002, Lonza). Nucleofection performed on program X-001 of Amaxa Biosystems Nucleofector II electroporator transfection unit ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 106 OSCs were transfected with 4 μg of pPB-TRE3G-FLAG-Rhino-Tjen-trTA-P2A-BlastR and 1 μg of pHsp70-Myc-hyPBase using Nucleofector Kit V (Lonza). After incubation in media containing blasticidin (50 μg/mL) ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines used are of human origin and were confirmed negative for mycoplasma before experimental use by using the MycoAlert Mycoplasma Detection Kit (Lonza) according to the the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Transfection of dissociated neurons was performed using the AMAXA Nucleofector system (program O.005) with the mouse neuron Nucleofector kit (Lonza) following the manufacturer’s specifications ...
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bioRxiv - Genomics 2023Quote: ... Neurons were nucleofected with 10 µ total DNA plasmid DNA using the AD1 4D-Nucleofector Y kit (Lonza V4YP-1A24) with the ED158 (iCell GlutaNeurons ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were co-transfected with 2 µg of the CRISPR/Cas9 plasmid and 2 µg of ssODN (50 µM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The genotypic identity of the parasites (28) and the absence of Mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza) were verified regularly ...
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bioRxiv - Cell Biology 2023Quote: ... and 2000 p mol of a degron-tagged control protein (mTagBFP2-RxxG) in a 100 µl nucleofection reaction (4D nucleofector kit SE plus supplement SF1, Lonza) 14 hours following nucleofection ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines used are of human origin and were confirmed negative for mycoplasma before experimental use by using the MycoAlert Mycoplasma Detection Kit (Lonza) according to the manufacturer’s specifications.
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bioRxiv - Genetics 2023Quote: LCLs were treated with 2nM Retinoic acid for 24 hours prior to transfection of firefly and renilla luciferase constructs by Amaxa Nucleofector kit V (Lonza) with the X001 program on a Nucleofector II device ...
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bioRxiv - Genomics 2023Quote: ... All cell lines were authenticated by short tandem repeat (STR) profiling and verified mycoplasma free using the MycoAlert PLUS Mycoplasma Detection kit (Lonza) prior to conducting experiments ...
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bioRxiv - Biochemistry 2023Quote: ... at 5% CO2 at 37 °C in accordance with standard mammalian tissue culture protocols and periodically tested for mycoplasma contamination via the MycoAlert Mycoplasma Detection kit (Lonza).
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bioRxiv - Cancer Biology 2023Quote: ... The virus product was tested to confirm sterility (Mayo Clinic Department of Laboratory Medicine and Pathology) and absence of endotoxin using the LAL-Kinetic QCL Kit (Lonza). Whole genome sequencing was performed by utilizing reverse transcription of purified viral RNA to cDNA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were treated with mycoplasma removal agent (MP Biomedical) and tested monthly for mycoplasma contamination using MycoAlert Plus mycoplasma testing kit (Lonza). SeV Cantell strain was grown in 10-day-old ...
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bioRxiv - Immunology 2024Quote: ... siRNA against human genes or control siRNA were incubated with a mixture of nucleofection solution (Human Monocytes Nucleofector kit; Lonza) and primary human monocytes and placed in nucleofection cuvettes subjected to program Y-010 for the Nucleofector 2b Device (Lonza) ...
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bioRxiv - Neuroscience 2023Quote: ... the presence of mycoplasma in the cultures was regularly screened using the MycoAlert™ Mycoplasma Detection Kit (#LT07-218, Lonza).
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were routinely screened throughout the study and found to be negative for mycoplasma with the MycoAlert detection kit (Lonza).
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bioRxiv - Cell Biology 2024Quote: ... 5 µg of pHTN HaloTag-CENP-A plasmid was used for transfection performed on Nucleofector Kit R with the Nucleofector 2b Device (Lonza) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The constructs were then transfected into WT OSCs and Δl(3)mbt-OSCs with Nucleofector Kit V (Lonza, VVCA-1003) using T-029 program and a NucleofectorTM 2b Device (Lonza) ...
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bioRxiv - Neuroscience 2024Quote: ... All fibroblasts and iPSCs were screened for mycoplasma monthly via MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza Cat # 75860-362). iPSCs were grown in medium-sized colonies on Matrigel (BD Biosciences cat# ...
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bioRxiv - Microbiology 2024Quote: ... Full mature schizonts were purified from highly synchronized parasites and then transfected with pFCEN-rif plasmids with a Amaxa nucleofector 2 with parasite nucleofector kit 2 (LONZA). Since the pFCEN-rif has the human dihydrofolate reductase as a drug selectable marker ...
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bioRxiv - Physiology 2024Quote: ... counted and 3.106 cells resuspended in 100 μl Nucleofactor R solution and electroporated with 3 µg of plasmid (pcDNA3 containing or not α7-5HT3 cDNA) using the Amaxa nucleofactor kit R (Lonza) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... ARPE-19 cells were maintained in Complete media containing DMEM: F12 supplemented (Lonza; Basel, Switzerland) + 10% FBS + 1% PEST + 2.5 mM L-Glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... Normal breast epithelial cells HMLE and MCF-10A were cultured in MEGM (Lonza CC-3151) containing SingleQuots Supplements (Lonza CC-4136) ...
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bioRxiv - Cell Biology 2019Quote: ... and X-001 (for Ba/F3 cells) according to the manufacturer’s recommendations (Lonza, Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: Normal human bronchial epithelial cells were purchased from Lonza (CC-2540S, single donor, lot # 493462) and grown in PneumaCult™-Ex Plus Medium (Stemcell Technologies 05040) ...
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bioRxiv - Developmental Biology 2021Quote: ... and human umbilical venous endothelial cells (HUVECs) (CC-2519A) were maintained in EGM2 media (Lonza). For siRNA ...
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bioRxiv - Immunology 2019Quote: ... and the red blood cells were removed by treatment with a hypotonic lysis buffer (Lonza). Cells were analyzed using flow cytometry ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 1 μg plasmid DNA was added and cells electroporated in a 4D-NucleofectorTM System (Lonza) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... ~1 μl of cells were mounted on EMM4S agarose pads (1.4% InCert™ Agarose, Lonza) and sealed under a coverslip with VALAP (Vaseline ...
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bioRxiv - Biophysics 2019Quote: Human pancreatic ductal adenocarcinoma Suit-2.28 cells (14) were cultured using RPMI 1640 medium (Lonza Walkersville Inc. ...
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bioRxiv - Immunology 2019Quote: ... Cells were then electroporated in 16-well strips in a 4D Nucleofector X unit (Lonza) with program EH-100 ...
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bioRxiv - Genetics 2020Quote: ... 250 ng of plasmid per 2 × 105 cells was transfected using Amaxa solution SF (Lonza) and program CA-138 ...
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bioRxiv - Cell Biology 2019Quote: ... U2OS osteosarcoma cells were cultured at 37 °C in DMEM (Dulbecco’s Modified Eagle Medium; Lonza) supplemented with 10 % foetal bovine serum (Lonza) ...
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bioRxiv - Immunology 2019Quote: ... B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza) containing 10% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Supernatant was aspirated and cells were carefully resuspended in P3 solution (Lonza, Cat. # V4XP-3012) containing the 20μg targeting vectors and 10μg pCAGGS-FLPe recombinase expression vector ...
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bioRxiv - Genetics 2019Quote: Lineage depleted cells were thawed via slow dropwise addition of X-VIVO 10 (Lonza, 04743Q) with 50% FBS (Sigma ...