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Citations for Lonza :
251 - 300 of 4401 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 105 to 2 × 105 cells were collected and resuspended in 15 µL of nucleofection buffer (Lonza). For each reaction ...
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bioRxiv - Cancer Biology 2023Quote: 1 x 103 cells per dish were plated onto 35mm dishes in 0.4% NuSieve Agarose (Lonza #50081). Six replicates of each condition were plated ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with pcDNA3.1 encoding full-length mouse PD-1 or its mutant using nucleofection (Lonza). Transfected cells were sorted for uniform PD-1 staining and culture in medium with 0.4 cells and reporter Jurkat cells stably expressing WT or MT chimeric PD-1 (mhPD-1 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were resuspended in 20 uL of the Amaxa NHDF Nucleofector Kit (Lonza, Walkersville, MD), containing 1ug of each of the gRNA and donor plasmid vectors (ABM ...
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bioRxiv - Microbiology 2023Quote: ... siRNAs (1 μg per ml) were nucleofected into ISE6 cells using the 4D-Nucleofector System (Lonza Bioscience). Tick cells were centrifuged at 100 x g for 10 minutes to pellet the cells ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were resuspended in 100 mL of the AmaxaTM NHDF Nucleofector kit (Lonza, Walkersville, MD), containing 1ug of each of the four episomal plasmid vectors encoding OCT3/4 and p53 shRNA ...
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bioRxiv - Cell Biology 2023Quote: ... Both ODMCs and VSMCs were cultured on 1% gelatin coated cell culture plates in SMGM2 medium (Lonza) at 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... 2.5×105-1×106 CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland, cat.: V4XP-3032) with complexed RNPs and electroporated using the Lonza 4D Nucleofector and 4D-Nucleofector X Unit (program DZ-100) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were split at a 1:2 ratio every 2 days by trypsinization (Lonza cat# CC-5012), followed by centrifugation at 200 x g for 3 min before resuspending and reseeding in EBM Basal Medium supplemented with the EGM Singlequots Supplement Pack ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and replaced by Ultradoma Protein-Free (LONZA). The cultured medium was harvested and replenished on the second ...
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bioRxiv - Genetics 2022Quote: ... membranes were stained with SYPRO-Ruby Protein Gel Stain (Lonza Rockland) to confirm equal loading ...
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Immunology 2021Quote: ... and the next day (day 1) sequentially seeding primary human lung microvascular endothelial cells (Lonza, CC-2527, P5) and primary human lung alveolar epithelial cells (Cell Biologics ...
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bioRxiv - Molecular Biology 2019Quote: ... a modified antisense or control oligonucleotide (1 μM) was transfected into 3 × 106 cells using Nucleofector technology (Lonza) 16–18 h before the assay ...
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bioRxiv - Genetics 2019Quote: ... Between 1×10^4 – 5×10^4 cells per condition were resuspended in 20μl of Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the Cas9 gRNA RNP complex ...
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bioRxiv - Immunology 2021Quote: ... Approximately 15,000 HMEC-1 cells/100 μl were seeded on coated wells using EGM-2 media (Lonza, Switzerland) and incubated overnight either with conditioned media from toxin-treated iPSC-MSC or vehicle at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Immunology 2022Quote: ... 1 x 10^6 purified naive T-cells were resuspended in 15µL buffer P3 + 5µL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7µL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Immunology 2020Quote: ... Cells were then washed with PBS and incubated in flu media containing 1% SeaPlaque agarose (Lonza, Basel, Switzerland). After 48hr incubation ...
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bioRxiv - Cell Biology 2020Quote: ... U–2–OS-pEP15 cells (5) were maintained in 1 mg/ml glucose phenol-red-free DMEM (Lonza) supplemented with steroid-free FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were resuspended in an equal volume of molten 1% InCert agarose (Lonza Rockland, Inc., Rockland, ME) in PBS ...
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bioRxiv - Immunology 2023Quote: ... 1-10 million of pre-activated P14 cells were resuspended in 20 μl of P4 Nucleofection Buffer (Lonza). This cell suspension was then mixed with sgRNA/Cas9 and then incubated at room temperature for 2 mins ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were used at passage 1-2 and were growth arrested in smooth muscle basal media (Lonza) supplemented with 0.3% FBS for 24-48 h before beginning experiments ...
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bioRxiv - Immunology 2024Quote: ... 1 - 2.5 × 106 Jurkat T cells were resuspended in EP buffer from the Nucleofector® Kit V (Lonza). Cells were combined with 1.5 μg of donor plasmid in a reaction volume of 100 μL and electroporated on the Amaxa® Nucleofector® II using pulse code X-005.
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bioRxiv - Immunology 2023Quote: ... 1×10e6 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Human Stem Cell Nucleofector Kit 1 (Cat. No. VPH-5012) and mESC Nucleofector Kit (Lonza, Cat. No. VVPH-1001) were used for delivering DNA into human and mouse ESCs ...
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bioRxiv - Neuroscience 2019Quote: ... 8 × 105 cells were co-transfected with 3 µg plasmid-sgRNA construct and ssODN (0.6 µM final concentration) with Amaxa Human Stem Cell Nucleofector Kit 1 (Lonza) and program A-023 for the Amaxa Nucleofector Device II ...
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bioRxiv - Molecular Biology 2019Quote: RPE-1 cells were transfected with 20 pmol siRNA by nucleofection (Amaxa Lonza 4D program EA-104 P3 solution) or Lipofectamine RNAiMAX transfection reagent (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... 1 million U2OS cells were transfected using the Nucleofector 2b with 2 μg Plasmid DNA using kit V (Lonza) according the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... 2 × 105 organoid single cells were re-suspended with Lonza P3 nucleofection buffer and 1 µl of pmaxGFP (Lonza) and transferred to 20 µl nucleofection cuvette (V4XP-3024 ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were transfected with 250 nM of siRNA and 1 μl of control pMax GFP (Nucleofector X kit, Lonza), using the CU-133 program ...
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bioRxiv - Immunology 2022Quote: ... and human monocytic leukemia cell line THP-1 (ATCC TIB-202) were cultured in RPMI-1640 (Lonza, 12-115F) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
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bioRxiv - Bioengineering 2019Quote: ... Transfection of transposon and transposase vectors was performed on day 2 with 0,5 µg transposon MC and 2 µg SB100X transposase RNA per 1×106 T cells on a 4D-Nucleofector according to the manufacturer’s instructions (Lonza). Transfected CD8+ and CD4+ T cells were expanded using the G-Rex culture system (Wilson-Wolf) ...
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bioRxiv - Cancer Biology 2020Quote: 1×105 cells were suspended in 1 ml of 0.33% select agar in small airway growth medium without EGF (Lonza) and plated on a bottom layer of 0.5% select agar in the same media in six-well dishes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells (1 × 106/10 cm dish) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; catalog no. 12-604F, Lonza) with 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with PBS and 1 ml of fresh Hepatocyte Culture Medium (HCM) was added per well (Lonza). At 6 or 72 hpi ...
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bioRxiv - Microbiology 2021Quote: Primary human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Cat# CC-2540, male; Lonza; Donor 1) or derived directly from a patient at Cambridge University Hospitals NHS Trust (Research Ethics Committee Reference 19/SW/0152 ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 T-cells were pelleted and re-suspended in 100 μL nucleofection solution (Lonza, Walkersville, MD, USA). T-cell suspensions were mixed with 5 μl RNP complex and cell/RNP mix transferred to Nucleofector cuvette ...
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bioRxiv - Bioengineering 2023Quote: ... single guide hybrids were mixed with 3uM Cas9 nuclease (Berkeley Labs) at a 1.2:1 ratio and delivered to cells by Lonza 3D (CA-137 ...
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bioRxiv - Neuroscience 2023Quote: Electroporation with freshly assembled RNP complex was performed on hiPSCs using the Human Stem Cell Nucleofector Kit 1 (Lonza) in conjunction with the Amaxa Nucleofector 2b system (Lonza ...
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bioRxiv - Immunology 2023Quote: moDCs were cultured with allogenic naïve CD4+ T cells in a 1:10 ratio in complete IMDM (IMDM (BE12- 722F, Lonza) with 100 U/mL penicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×10E6 ME-1 cells were nucleofected with CRISPR/Cas9 plasmids (2 μg each) using Nucleofector Technology (Lonza Biologics) with the program X-01 and Amaxa Cell Line Nucleofector Kit V ...
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bioRxiv - Immunology 2023Quote: ... The sgRNA complexes were then added to 1×106 NK92 cells resuspended in 16uL of P3 nucleofection buffer (Lonza). The nucleofection mixture was transferred to a 16-well strip for nucleofection in the Lonza 4D Nucleofector using the pulse code CM-138 ...
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bioRxiv - Bioengineering 2023Quote: ... with two phosphothiorate linkages on each end were mixed with 82 µL Nucleofector Solution V and 18 µL of Supplement Solution 1 according to manufacturer’s recommendations for the Cell Line Nucleofector Kit V (Lonza). 5×105 U2OS.EGFP parent cells were resuspended in the above mixture and nucleofected with a Lonza Nucleofector 2b Device (Kit V ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL of cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% Ultraglutamine 1 (Lonza), 1% B27 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... the RNP complexes were added to 1×105 cells in 40 μl of P3 nucleofection buffer from the 4D-Nucleofector X kit (Lonza). Half of the mixture was loaded to each well of a 16-well nucleofection cassette and nucleofected using the using E0-100 program with the 4D-Nucleofector Lonza Amaxa ...
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bioRxiv - Genomics 2020Quote: ... Transfection of these iPSCs with the plasmid and Super piggyBacTM transposase mRNA (Transposagen) was done using the Human Stem Cell Nucleofector Kit 1 (VAPH-5012) by Nucleofector 2b Device (AAB-1001, Lonza) according to the manual ...