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Citations for Lonza :
2751 - 2800 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM sodium pyruvate (Lonza, Basel, Switzerland), and 55 nM β-mercaptoethanol (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: ... and 1× non-essential amino acids (Lonza) at 37 °C and 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... and streptomycin (10,000 IU mL-1, Lonza). Vero E6 cells were inoculated at an moi of 0.01 ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 mg ml-1 sodium bicarbonate (Lonza) and 10 mM Hepes (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... 1% Non-Essential Amino Acid Solution (Lonza), 1% Sodium Pyruvate (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... and streptomycin (10,000 IU mL −1, Lonza) at 37°C in a humidified CO2 incubator ...
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bioRxiv - Genetics 2020Quote: ... and 1% penicillin/ streptomycin (Lonza, catalog DE17602E). The cells were passed twice per week at a density lower than 0.5 × 106 cells/ml in a humidified incubator at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... and 20 µg ml-1 trypsin (Lonza). MDCK cells were inoculated at an moi of 0.01 ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 mg ml-1 sodium bicarbonate (Lonza), 10 mM Hepes (Lonza) ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 mg ml-1 sodium bicarbonate (Lonza), 10 mM Hepes (Lonza) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3.6 Supplement 1 (Lonza kit, #V4XP-3032), and 1ug of plasmid DNA or 200pmol of chemically stabilized synthetic RNA (Synthego Corp.) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 U ml−1 Penicillin-streptomycin (Lonza) and 5 mM sodium pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... 1% penicillin-streptomycin mix (Lonza, DE17-602E). HCT116 and U2OS cells were grown in McCoy’s medium (Lonza ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM sodium pyruvate (Lonza 13-115E), and 100 U/mL penicillin-streptomycin was used for imaging.
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bioRxiv - Cancer Biology 2023Quote: ... and 1% non-essential amino acids (Lonza) at 37 °C in a 5% CO2 humidified environment.
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bioRxiv - Immunology 2023Quote: ... 1 mM sodium pyruvate (Lonza, BE13-115E), and 10% MCSF conditioned media ...
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bioRxiv - Cell Biology 2024Quote: ... 1% (v/v) L-glutamine (Lonza, UK), 10 μg/mL gentamicin ...
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bioRxiv - Immunology 2024Quote: ... and 1% Pen-Strep (DE17-602E; Lonza). Dynabeads Human T-Activator CD3/CD28 (11161D ...
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bioRxiv - Immunology 2024Quote: ... 100 µg ml−1 amphotericin B (Lonza) and 200 nM L-Glutamine (Gibco) ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
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bioRxiv - Immunology 2020Quote: ... followed by washing in complete media (RPMI-1640 containing 10% FBS, 2 mM glutamine, 100 IU/ml penicillin, and 100 µg/ml streptomycin, Lonza, Walkersville, MD, USA). Isolated BAL cells (approx.1-2 x106 ...
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bioRxiv - Molecular Biology 2024Quote: ... transfecting ∼5×106 cells in 1x Tb-BSF buffer (Schumann Burkard et al., 2011) using 2 mm cuvettes (BTX) with an Amaxa Nucleofector 2b (Lonza, program X-001).
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bioRxiv - Bioengineering 2023Quote: ... in 6-well tissue culture plates and were allowed to adhere for 5 hours before induction of serum starvation in EBM-2 (LONZA, Cat# CC-3156) for 16 hours prior to being administered media of interest ...
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bioRxiv - Microbiology 2022Quote: ... Cells were overlaid with 100 μl/well of the 1:1 (v/v) mixture of 2.4% Avicel (DuPont) and 2XEMEM (Lonza) with 4% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y neurons (ATCC) were cultured in a 1:1 mixture of Eagle’s Minimum Essential Medium (EMEM, Lonza BioWhittaker) and F12 Medium (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... 8×107 promastigotes were transfected with 1 µg (≈ 1,68.1011 molecules) of the linearized barcoding library using the Basic Parasite Nucleofector™ kit 1 (Lonza) with the U-033 program ...
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells (grown in 6-well dishes) were subjected to nucleofection with Amaxa® Cell Line Nucleofector® kit V (VCA-1003, Lonza), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 bulk or stable populations overexpressing Nluc-CD63 or Nluc-CD9 constructs were obtained using an electroporation-based transfection protocol optimized for this cell line (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... subjected to red blood cell lysis with ACK lysis buffer and electroporated with P3 Primary Cell 4D-NucleaofactorTM X -kit (V4XP-3032, Lonza, Basel, Switzerland), as previously described (Freund et al. ...
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bioRxiv - Cell Biology 2021Quote: ... We transfected the KCNQ1 constructs and the empty vector into our established mouse pancreatic β cells (referred to as SJ β cells (Jia et al., 2015)) using AmaxaTM Cell Line NucleofectorTM Kit V and AmaxaTM NucleofectorTM 2b device (Lonza, Switzerland). The glucose-sensitive mouse β-cell line SJ-β was cultured and an insulin-secretion assay performed as reported earlier (Jia et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and used to infect fresh Sf9 cells (1.2 × 106 cells* ml) in 10 ml of Insect-Xpress medium (Lonza, Walkersville, MD, USA) with 15 mg/ml gentamycin (Roth ...
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bioRxiv - Cell Biology 2022Quote: ... 1uM siRNA was electroporated into cells using the Amaxa Nucleofector II device and Cell Line Nucleofector Kit V (Lonza Bioscience, VCA-1003). B16-F10 cells were electroporated with program P-020 ...
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bioRxiv - Microbiology 2021Quote: ... the Sleeping Beauty transposase system [37] was adapted to electroporate Jurkat cells using the Lonza SE Cell Line kit (Lonza, V4SC-1960). The pSBbi-RP plasmid was a gift from Eric Kowarz (Addgene plasmid #60513 ...
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bioRxiv - Immunology 2019Quote: ... conjugated to FITC or GFP siRNA (300 nM) conjugated to FITC two days prior to adoptive T cell therapy using the Mouse T cell Nucleofector™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... as a control two days prior to adoptive T cell therapy using the P3 Primary Cell 4D-Nucleofector X™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and the GFP-K17ΔNLS (Hobbs et al., 2015) were nucleofected into HeLa cells using SE Cell Line 4D X nucleofector Kit S (Lonza #V4XC-1032) with setting DS-138 ...
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bioRxiv - Immunology 2021Quote: ... gRNA_pX335 plasmids were transfected into EL4 cells by nucleofection using an Amaxa nucleofector and the Cell Line Nucleofector Kit L (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were seeded onto 96-well plates at a density of 3×104 cells per well in 100 μL cell culture media that consisted of DMEM (Lonza, Basel, Switzerland), 1% penicillin–streptomycin (Lonza ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and sgRNAs (see Key Resources Table) were delivered by nucleofection to a single cell suspension of 105 iBCs in Primary Cell P3 solution (Lonza, #V4XP-3032). Cells were re-cultured and the resulting clonally-derived spheroids were isolated ...
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bioRxiv - Cancer Biology 2024Quote: Bone marrow was harvested from 8–12-week-old female B6 or BALB/c mice and processed into a single cell suspension and red blood cells were lysed with ACK lysis buffer (Lonza, Walkersville, MD). NK cells were isolated by magnetic cell separation with a NK cell isolation kit and an AutoMACS Pro (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Developmental Biology 2024Quote: ... cell pellets of 200x103 cells were resuspended with 20 µL of supplemented nucleofector solution P3 Primary Cell 4D-Nucleofector® S (Lonza, V4XP3032) with indicated amounts of multi-guide sgRNAs (Synthego ...
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bioRxiv - Genomics 2024Quote: ... were complexed for 20 minutes at room temperature and were nucleofected into 5E5 PEmax parental cells using the SE Cell Line 4D-Nucleofector X Kit (Lonza V4XC-1032) and program FF-120 ...
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bioRxiv - Bioengineering 2023Quote: ... MCF-10A cells were cultured using an MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ without gentamycin-amphotericin B mix (Lonza) following ATCC’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CRISPR-CAS9 knockout in SU-DIPGXIII cells was performed using the Amaxa P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-3012). First ...
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bioRxiv - Cancer Biology 2022Quote: ... and BEBM™ Bronchial Epithelial Cell Growth Basal Medium supplemented with the BEGM™ Bronchial Epithelial Cell Growth Medium Single Quots™ Supplements and Growth Factors (Lonza). Fresh media were added to the cells every 2-3 days until the confluent monolayer of mesothelial cells was obtained (typically between 5-10 days post-isolation).
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bioRxiv - Neuroscience 2023Quote: ... 8 x 105 hiPSCs in single-cell suspension were nucleofected with 5 μg SpCas9-sgRNA plasmid using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, #V4XP-3024) in combination with the 4D Nucleofector Unit X (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... ePB master vector and helper (Plasmid AW-27) were nucleofected into the established SOX2::mCitrine cell line using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza; V4Xp-3012). G-418 (40ng/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... MV411 cells were electroporated with Cas9/sgRNA complexes targeting the HDR insertion using a Lonza SF Cell Line 4D Nucleofector (Lonza V4XC-2032). RNP complexes were formed by mixing 8.5 μg of TrueCut Cas9 Protein v2 (Invitrogen A36499 ...