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Citations for Lonza :
2551 - 2600 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Human visceral pre-adipocytes were purchased from Lonza (PT-5005) and cultured according to the manufacturer’s instructions in PGM-2TM Preadipocyte Growth Medium-2 BulletKitTM from Lonza (PT-8002). Once cells were expanded ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 2% haematocrit in O+ erythrocytes and RPMI-1640 medium (Lonza, BE12-167F or Gibco, 31870-025) supplemented to give the following final concentrations ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza) ...
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bioRxiv - Microbiology 2020Quote: ... Infected CD4 T cells were washed with PBS and 3 million cells per condition were resuspended in 20uL buffer P2 (Lonza) with 4μM IDT electroporation enhancer ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Genomics 2020Quote: ... 3 μL RNP complex solution was mixed with the cells and the cells were nucleofected using program DJ-110 on a 4D-Nucleofector (Lonza) with 16-well strips ...
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bioRxiv - Cancer Biology 2020Quote: ... HMLE RAS Twist and HMLE RAS Twist ID1 cells were described previously (Stankic et al., 2013) and grown in MEBM (Mammary Epithelial Cell Growth Medium) (Lonza), supplemented with BPE (Bovine Pituitary Extract ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution electroporation cuvettes (Lonza). Electroporation was performed according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Single cell suspensions were prepared by mechanical dissociation and expelling the cells through 40 μ using ACK lysing buffer (Lonza). Cells were washed and stained with an antibody cocktail of CD150 ...
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... The RNP complex and 1.5 μg of a linearized homology-directed repair template plasmid were transfected into 2×105 – 5×105 nocodazole-arrested mitotic HeLa A12 cells using a Nucleofector and the associated Cell Line Kit R (Lonza) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Raw264.7 Ifit2 CRISPR cells were generated by electroporating low passage Raw264.7 cells with Ifit2 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...
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bioRxiv - Molecular Biology 2020Quote: ... H69 cells at 10,000 cells per well were mixed with 0.3% low melting temperature agarose (NuSieve GTG) (Lonza, Walkersville, MD) in complete medium ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Biophysics 2022Quote: ... 4-6 µg of DNA was mixed with the resuspended cells and electroporated using the Amaxa cell nucleofector (Lonza Laboratories) program Q-001 ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were introduced into HCT116 and MDCK cells using nucleofection with the 384-well HT Nucleofector and the SE cell line kit (Lonza) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: HT29-N2 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) cell culture media (Lonza. Cat. Nº: BE12-604F/U1) supplemented with 10% (vol / vol ...
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bioRxiv - Bioengineering 2019Quote: ... approximately 200,000 cells were resuspended in a mixture containing 20 µl of P3 Primary Cell Nucleofector™ solution (V4XP-3032, Lonza), 5 µl of RNP complex for each guide (both guides AC and AD ...
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bioRxiv - Synthetic Biology 2019Quote: U2OS cells stably expressing eGFP.PEST or MDA-MB-231 cells were transfected with Cas9 ribonucleoprotein (RNP) and ssODN using the SE Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of DN-100 (U2OS ...
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bioRxiv - Cell Biology 2021Quote: ... was generated by the introduction of a plasmid carrying the EGFP gene under the control of the CAG promoter to EStTA5-4 cells using the Amaxa Mouse ES cells Nucleofector Kit (VPH-1001, Lonza).
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bioRxiv - Cell Biology 2021Quote: ... the culture medium was removed and the cells were replenished with UltraCULTURE serum-free cell culture medium (Lonza, BE12-725F). Medium containing secreted lentivirus was collected every 24 hours for a total of 72 hours and stored at 4°C ...
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bioRxiv - Immunology 2020Quote: Targeted gene’s expression was knocked-down in up to 5 x 106 cells using the P3 Primary Cell 4D-Nucleofector X Kit L (V4XP-3024, Lonza) with 90 µl P3 Primary cell solution and 100 pmol of corresponding si_RNA (resuspended in 10 ul RNAse-free H2O) ...
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bioRxiv - Cancer Biology 2020Quote: ... DRGFP expressing cells were electroporated in reagent T for H520 and H2228 or reagent L (Amaxa Cell Line Nucleofector Kit L, Lonza) for Calu6 with 10ug I-SceI using program X-005 for Calu6 ...
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bioRxiv - Systems Biology 2020Quote: 5×105 HAP1 cells were transiently transfected with 300 ng of plasmid using the SE cell line 4D-Nucleofector kit S (Lonza), on a 4D nucleofection unit (Lonza ...
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bioRxiv - Microbiology 2021Quote: Vero E6 cells (C1008; green African monkey kidney cells) were obtained from Public Health England and cultured in DMEM medium (Lonza) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... and 5×106 cells seeded in 100-cm2 cell culture dishes in 12 mL RPMI supplemented with 10% fetal calf serum (Lonza), 1X non-essential amino acids (NEEA ...
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bioRxiv - Immunology 2021Quote: ... the T cell / RNP / HDRT mixture was transferred onto a 16-well electroporation strip (20 µl = 106 cells per well, Lonza) or an electroporation cartridge (100 µl = 5×106 cells ...
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bioRxiv - Biochemistry 2022Quote: ... washed once with phosphate-buffered saline (PBS) and resuspended at 107 cells/mL in P4 Primary Cell Nucleofector Solution (Lonza). 20 µL of resuspended cells (corresponding to ~ 200,000 cells ...
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bioRxiv - Biophysics 2022Quote: ... The transfected cells were then co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) and cultured until virus-mediated CPE was observed ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF10A cells were purchased from ATCC and cultured with MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ (Lonza/Clonetics) supplemented with not supplemented with 100 ng/ml cholera toxin (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... and irradiated allogeneic PBMC (50 Gy) pooled from three donors as feeder cells in T-cell medium RPMI 1640 plus (Lonza):AIM-V (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... the cell suspension was passed through 35 µm Cell Strainer Snap Cap and treated with red blood lysis (ACK buffer, Lonza) for 2 min at room temp ...
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bioRxiv - Cancer Biology 2024Quote: ... Single-cell suspensions were spun down and tumor cells were lysed with ACK RBC lysis buffer (Lonza Group, Basel, Switzerland) at room temperature for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... twice and resuspended in Cell Line Nucleofector solution SF (16.4uL) with Supplement (3.6uL) (SF Cell Line 4D-nucleofector X Kit, Lonza, V4XC-2032). Alt-R SpCas9 nuclease (100pmol ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in respective electroporation/nucleofection buffer (HEK293T cells and HepG2 SF Buffer (SF Cell Line 4D-Nucleofector X Kit, Lonza), Jurkat cells SE buffer (SE Cell Line 4D-Nucleofector X Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... The immortalized human mammary epithelial cells expressing OHT-inducible Twist (HMLE-Twist-ER) and Naturally Arising MEsenchymal Cells (NAMECs) were maintained in MEGM (Lonza) growth medium ...
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bioRxiv - Microbiology 2023Quote: Constructed plasmids encoding AS1-S were introduced into MDBK cells using Amaxa cell line Nucleofector kit R (Lonza, Kanagawa, Japan) with Amaxa Nucleofector II system in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... isolated WT P14 CD8+ T cells were washed with PBS and mixed with RNPs by using P3 Primary Cell 4D-NucleofectorTM X Kit (Lonza) immediately prior to electroporation (Lonza 4D-nucleofactorTM core unit ...
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bioRxiv - Genomics 2023Quote: ... 2.5e5 RPE1 CRISPRi cells were nucleofected with 3μM RNP complex using an SE Cell Line 4D X Kit S (Lonza Bioscience) on a 4D-Nucleofector (Lonza Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... T cells were washed with PBS and resuspended at 100x106 cells/mL in Lonza P3 Primary Cell 4D Nucleofector Kit buffer (Lonza). 1μg/1x106 cells of sgRNA (Agilent ...
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bioRxiv - Genetics 2023Quote: ... and cells were resuspended according to the protocol provided for the P3 Primary Cell 4D-Nucleofector Kit (Lonza, V4XP-3024). Briefly ...
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bioRxiv - Microbiology 2023Quote: Primary monocytes (3 × 106 cells/transfection) were washed with 1X PBS and resuspended in 100 μl of P3 Primary Cell Nucleofector solution (Lonza) containing either 1000 ng empty vector (EV ...
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bioRxiv - Cancer Biology 2023Quote: Cas9-RNPs were transfected into cells by electroporation (SF Cell Line 4D-Nucleofector™ X Kit S (Lonza, #V4XC-2032)) ...
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bioRxiv - Molecular Biology 2023Quote: Transfections of K562 cells were performed using a Lonza Bioscience 4D-Nucleofector system and the SF Cell Line 4D-Nucleofector X kits (Lonza). For single nucleocuvettes (100 uL) ...
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bioRxiv - Cell Biology 2023Quote: Differentiated HL-60 cells were nucleofected with Amaxa Nucleofector II device and Amaxa Cell line kit V (Lonza, VACA-1003) and prepared for live-cell imaging using aslightly modified version of an existing protocol 96 ...
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bioRxiv - Cancer Biology 2023Quote: ... Two gRNA plasmids and pCas9 (D10)-GFP plasmid were nucleofected into cells using Basic Nucleofector Kit for Primary Mammalian Epithelial Cells (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and co-transfected with an ssODN donor template containing the desired modification into H9/WA09 cells via nucleofection (Amaxa P3 primary cell 4D nucleofector X kit L, Lonza) using the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2023Quote: CD4+T cells were cultured immediately after isolation in 24-well plates (106 cells/well) containing RPMI 1640 medium (Lonza) with 10% FCS ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was used for nucleofection using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-3024), a 4D-nucleofector core unit and X unit (Lonza ...