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Citations for Lonza :
2701 - 2750 of 2765 citations for Estradiol Serum ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Beads were mixed at a 1:1 ratio with cells and cells were cultured at a density of 1 x 106 cells/mL in complete X-Vivo 15 culture media (Lonza, 5% fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were edited 3-5 days after isolation or thawing using the Lonza Nucleofector 4D (program EO-117) using 1×106 cells per well of a 16-well Nucleocuvette Strip (Lonza). Immediately following nucleofection ...
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bioRxiv - Neuroscience 2023Quote: Larvae were anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate salt (Tricane, (E10521, Ethyl 3-aminobenzoate methanesulfonate) and mounted in 1% low melting point agarose (50100, Lonza) in a 60 mm x 15 mm petri dish ...
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bioRxiv - Cancer Biology 2023Quote: Cell and organoid lines were generated from C3-TAg tumors (supplementary file 1) and tested for mycoplasma (Lonza, LT07-703) prior to use and the creation of frozen stocks ...
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bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Measured tissues originated from six F508del homozygous patients (CF patient #1: CF-AB0609, female, 31 years-old; CF patient# 3: #28388-0000450918, Lonza, male ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 100 μL of YEA medium containing 1% (w/v) microwave-heated low-melting point agarose (SeaPlaque agarose, 50101; Lonza) was gently added to the central area ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... HUVECs and HMVECs were cultured on 1% gelatin coated dishes in EGM-2 or EGM-2 MV (Lonza, Basel, Switzerland), respectively ...
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bioRxiv - Genomics 2023Quote: ... of the PCR products were analyzed by electrophoresis on a 2% Agarose S gel (Nippon gene) in 1× TBE buffer and stained with GelStar (Lonza). The remaining PCR product of the condition that displayed a single-band or near single-band product on the gel was double size-selected and purified using AMPure XP beads (Beckman coulter ...
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bioRxiv - Neuroscience 2023Quote: ... and the ssODN (1 μl; stock 100 μM, IDT) in 20 μl of nucleofection buffer P3 (P3 Primary Cell NucleofectorTM Solution, Lonza) were nucleofected (program CA137 ...
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bioRxiv - Neuroscience 2023Quote: ... reprogramming was initiated by nucleofection of 1×105 fibroblast with 1 µg of each episomal plasmid (pCXLE-hUL, pCXLE-hSK and pCXLE-hOCT4) using the Nucleofector 2b (Lonza). Initially ...
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bioRxiv - Molecular Biology 2023Quote: ... MRTF-WT and MRTF knockout in NIH 3T3 (MRTF-KO-1 and MRTF-KO-2) cells were cultured in DMEM (BE12-614Q, Lonza) supplemented with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... A cell count ranging from 1 × 10^5 to 2 × 10^5 cells was collected and suspended in 15 µL of nucleofection buffer (Lonza). Electroporations were conducted in the strip format ...
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bioRxiv - Immunology 2023Quote: ... CTLs derived from LifeAct-GFP expressing pmel-1 mice were incubated with peptide-loaded and IFNγ-treated B16 cells cultured in phenol red-free DMEM (Lonza) supplemented with 10% FBS on stage at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... An mTmG lens carrying the MLR39-Cre allele was placed in a small hole in the center of an agarose pad that was prepared by loading 1 mL of 2% agarose (SeaKem GTG, Lonza) in M199 on the surface of 35 mm glass-bottom dish (3970-035 ...
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bioRxiv - Cell Biology 2023Quote: ... and performed nucleofection where 1 × 106 of the selected clone were resuspended in 100 μl of P3 buffer (V4XP-3024, Lonza) containing 20 μg Alt-R® S.p ...
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bioRxiv - Biophysics 2024Quote: ... or 5-twist alone) were isolated by the separation of nicked knot reactions in 1% low-melting point agarose gels (SeaPlaque agarose, Lonza), followed by excision of specific bands ...
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bioRxiv - Biochemistry 2024Quote: Agarose gel electrophoresis was run in horisontal mode using a home-built apparatus as described.39 All the samples and the gels (1% (w/v) Sea Kem LE Agarose from LONZA Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mg Cas9-gRNA-2 plasmid and 1 mg eGFP-puromycin resistance plasmid using the Amaxa 4D-Nucleofector system (Lonza) according to the manufacturer’s instructions with a pulse load of CA137 ...
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bioRxiv - Bioengineering 2019Quote: ... The plate containing the amplified genes or qPCR products was kept in ice and observed in a 2% agarose gel (NuSieve® 3:1 Agarose (Lonza)) to check and reinforce the identity of the amplicons ...
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bioRxiv - Microbiology 2019Quote: ... the antibody-virus mixture was aspirated and Vero cells were washed with PBS and overlaid with DMEM containing 2% heat-inactivated FBS and 1% SeaPlaque Agarose (Lonza, 50501). After 4–6 days ...
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bioRxiv - Plant Biology 2021Quote: ... pollen was collected immediately prior to bombardment and distributed on pollen germination medium solidified with 1% (w/v) NuSieve GTG Agarose (Lonza, Switzerland). Pollen germination media for Nicotiana (Wang and Jiang 2011 ...
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bioRxiv - Microbiology 2021Quote: ... Vero cell cultures were seeded at 1-3 × 104 cells/cm2 in Dulbecco’s minimal essential medium (DMEM, LONZA, Alpharetta, GA, USA) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... or in Binding buffer (1% w/v BSA and 25 mM HEPES pH 7.2 in DMEM without phenol red; Lonza Netherlands B.V.) at 4°C in the case of HeLa cells ...
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bioRxiv - Genomics 2022Quote: ... 20 ng of HMW DNA was run on a 1% agarose gel (Seakem Gold Agarose, Lonza, Rockland, ME, USA, Cat #50150) in 0.5xTBE with the BioRad CHEF Mapper system (BioRad ...
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bioRxiv - Immunology 2020Quote: ... The inoculum was then removed and replaced with 2 mL of complete DMEM medium with 1% SeaPlaque agarose (Lonza, Cat# 50100). The plate was incubated at 37°C and 5% CO2 for 3 days ...
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bioRxiv - Genomics 2019Quote: ... DNA fragments underwent PCR-based amplification using Illumina’s Nextera® primers and the resulting libraries were subjected to electrophoresis for the indicated time in 1% agarose gels (Lonza) at 3.0 V cm-1 in TBE buffer (50 mM Tris-borate ...
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bioRxiv - Cell Biology 2020Quote: ... and DMEM with recommended Lonza supplements (1 supplement pack per 500 mL of media) with additional bovine pituitary extract (12.6 μg/mL, Lonza and AthenaES), BSA (final concentration of 1.5 μg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 ng/mL and 0.5 µg/mL or 1 µg/mL were used in Mammary Epithelial Growth Medium (Lonza, CC-3150), respectively ...
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bioRxiv - Bioengineering 2022Quote: ... Deep Blue was prepared at a ratio of 1:10 with colourless DMEM (without pyruvate, with 4.5 g/L glucose (Lonza, Basel, Switzerland)) and was added to each existing well and incubated at 37 °C for 3 h ...
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bioRxiv - Physiology 2022Quote: ... (5 g/L trypsin and 2 g/L EDTA diluted 1 in 5 in calcium- and magnesium-free PBS) (Lonza, UK).
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bioRxiv - Molecular Biology 2023Quote: ... The integrity of RNA was verified by resolving in 1% agarose gel containing GelStar nucleic acid gel stain (catalog no. 50535, Lonza, USA) in a Mupid-One gel electrophoresis system (TaKaRa ...
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bioRxiv - Microbiology 2023Quote: ... 0.4 µg/ml hydrocortisone (Upjohn 100mg SERB), 0.5 µg/ml insulin (Novo Nordisk, Novorapid Flexpen) and 1× penicillin/streptomycin (LONZA, Verviers, Belgium). The flasks were incubated at 37°C with 5% CO2 in a humidified incubator ...
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bioRxiv - Microbiology 2023Quote: ... Cells were co-incubated with virus and then supplemented with overlay medium that contains 1% SeaPlaque agarose (Lonza Bioscience, Basel, Switzerland). After 2 days of incubation ...
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bioRxiv - Genomics 2023Quote: ... ATCC #CRM-CRL-1420) and PANC-1 cells (CVCL_0480, ATCC #CRM-CRL-1469) were cultured in D10 media: DMEM (Lonza #12-614Q) supplemented with 10% FBS (GELifeSciences #SH30071.03) ...
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bioRxiv - Immunology 2023Quote: ... vectors was performed on day 2 of activation into 1×106 T cells using 4D-Nucleofector according to the manufacturer’s instructions (Lonza, Cologne, Germany).
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bioRxiv - Genetics 2023Quote: ... The pellet was washed with 10 ml of 1 x PBS and resuspended in 20 µl of nucleofection SF buffer (Lonza Biosciences), in which 600 ng of the DsRed2-N1 plasmid was added to the suspension ...
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bioRxiv - Biochemistry 2023Quote: Phenol-red free complete working medium (PRF-CWM) was comprised of DMEM (874.1 mL·L−1; DMEM; BioWhittaker®; Lonza; Walkersville; USA), foetal bovine serum (104.9 mL·L−1 ...
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bioRxiv - Neuroscience 2024Quote: ... each consisting of 28 chambers connected to an independent computer.All tastants used were solved in water 1% low melt agarose (Lonza, ref. 50100). To transfer files to each chamber ...
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bioRxiv - Immunology 2024Quote: ... One million NK cells were resuspended in 20 ul 4D nucleofector master mix (82% P3 + 18% supplement 1; Lonza, V4XP-3032), and mixed with Cas9 RNP for electroporation using program CM137 ...
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bioRxiv - Cancer Biology 2021Quote: ... To generate IKZF3-deficient CAR-T cells, 15-20 million PBMCs in RPMI 1640 (10% FBS, 1% penicillin/streptomycin) were nucleofected using Nucleofector 2b (Lonza, Köln, Germany) with a mixture of 4 plasmids (5 μg each ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were electroporated with 10 µg lineage recorder DNA and 1 µg Sleeping Beauty transposase in 100 µl Human Stem Cell nucleofection buffer (Lonza, VVPH-5022) using the B-016 program of the 4D-Nucleofector (Lonza ...
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bioRxiv - Neuroscience 2020Quote: ... 20 pmol Cas9 nuclease (sgRNA to Cas9 nuclease ratio used as 9:1) was performed with Amaxa 2D Nucleofector (Lonza, program B016). After recovery ...
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bioRxiv - Immunology 2019Quote: ... were sorted into B cell media (IMDM medium, GIBCO; 10% heat-inactivated low IgG FBS, Life Technologies; 5 ml GlutaMAX, Life Technologies; 1 ml MycoZap plus PR, Lonza). Immediately following the sort ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza, Walkersville, MD, USA). The generation number of ECs is maintained between 30-40 generations ...
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bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
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bioRxiv - Microbiology 2021Quote: ... were isolated by Ficoll density gradient centrifugation as described previously [22] and seeded at a density of 1×106 cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Systems Biology 2022Quote: ... The PGK reporter cell line was generated by electroporation of K562 cells with 0.5 ug each of plasmids encoding the AAVS1 TALENs and 1 ug of donor reporter plasmid using program T-016 on the Nucleofector 2b (Lonza, AAB-1001). Cells were treated with 0.5 ug/mL puromycin for one week to enrich for successful integrants ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Genetics 2024Quote: ... erythroleukemia K562 cells were washed in 50 mL PBS at RT and approximately 1×106 cells were resuspended in SF solution (Lonza V4XC-2032) and electroporated in presence of pre-assembled Cas9:gRNA complexes plus 12 µg ssDNA (100 µL format ...