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Citations for Lonza :
2551 - 2600 of 2765 citations for Estradiol Serum ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
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bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Developmental Biology 2022Quote: Generation of Foxa2 knockout cell lines was performed by electroporating two different guides cloned into the pX458 vector (Ran et al., 2013) with the AMAXA nucleofector kit (Lonza Cat no. VPH-1001). Cells were sorted for GFP as single cells one day after electroporation ...
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Cancer Biology 2019Quote: ... and 3-4 hours later were transfected with 2 μg of pBabe KRAS WT and pBabe KRAS G12D or pBabe KRAS Q61H plasmid DNA by electroporation using the Amaxa® Human T Cell Nucleofector® Kit (Lonza, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The cell line was authenticated through short tandem repeat analysis and tested for mycoplasma contamination every 10 passages using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). PDGFR-BiFC and myr-Venus stable cells were cultured in RPMI growth medium [RPMI 1640 (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were cultured at 37°C and 5% CO2 and tested for mycoplasma contamination using a MycoAlert® mycoplasma detection kit (Lonza, cat: LT07-218). Only mycoplasma-negative cells were used.
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bioRxiv - Bioengineering 2023Quote: The murine myoblast cell line C2C12 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was tested for mycoplasma (MycoAlert™ Mycoplasma Detection Kit, Lonza AG, Basel, Switzerland). Cells were cultured in monolayer at 37 °C in a 5% CO2-containing humidified atmosphere in complete growth medium (GM) ...
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bioRxiv - Cancer Biology 2023Quote: NPE cells were transfected by electroporation using a Lonza® Nucleofector 2b device and the Lonza® Mouse Neural Stem Cell Nucleofector Kit (Lonza; #VPG-1004) according to the manufacturer’s instructions using the T-030 pulse code.
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bioRxiv - Cancer Biology 2023Quote: CD4 SMARTA T cells were isolated from naïve mice and were washed and resuspended in 20 μl buffer P3 (P3 Primary Cell 4D-Nucleofector X Kit S, Lonza, cat. no. V4XP-3032). Between 105 to 107 cells were used per nucleofection ...
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bioRxiv - Synthetic Biology 2023Quote: ... CD22-deleted clones modified to express PD-L1 (LZRS PD-L1) or CD200 (PB-EF-1α-intron CD200, pCMV-hyPBase, SF buffer kit V4XC-2032, Lonza 4D Nucleofector, code DN100). Transposition reactions used 1-2 μg of vector DNA and 0.5-1.0 μg of pCMV-hyPBase transposase DNA ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were transfected with the pre-assembled fRNP pool by electroporation (program: OP315 or CD112) using an SE Cell line 4D-Nucleofector™ X kit (Lonza, Catalog#: V4XC-1024). The electroporated cells were plated in Nunc Glass Bottom Dishes (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... All iPSCs were karyotypically validated via g-banded karyotyping (WiCell) and are regularly screened for mycoplasm via MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza Cat # 75860-362). Two major approaches to generating neurons in a dish are based on overexpression of proneural factors combined with chemicals from iPSCs differentiation or directly converted from fibroblasts ...
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bioRxiv - Cell Biology 2023Quote: ... to aid complete removal of PBS before resuspension in P3 Nucleofection Solution (room temperature; P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza; cat. no., V4XP-3032). A total of 20 μl solution was used to resuspend 80,000 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained at 37°C with 5% CO2 and were regularly tested for Mycoplasma contamination using the MycoAlert PLUS kit (Lonza, Cat. No. LT07 −705).
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bioRxiv - Molecular Biology 2024Quote: ... counted and 1×106 cells electroporated using the CB-150 program of the 4D-Nucleofector™ System and the P3 Primary Cell 4D-Nucleofector® X Kit L (Lonza, #V4XP-3024) according the manufactures protocol and plated in E8+RI ...
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bioRxiv - Immunology 2024Quote: ... For transient transfection 1e6 Jurkat cells were transfected with 2 μg DNA using the LONZA electroporator (program X-001) and Nucleofector Kit V (Lonza, catalog no. VCA-1003) 24 h prior to imaging ...
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bioRxiv - Cancer Biology 2022Quote: ... where 1×106 4T1 cells in 50 µL of phosphate-buffered saline (PBS, Lonza, cat. 17-512F) were subcutaneously (s.c. ...
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bioRxiv - Molecular Biology 2019Quote: ... 75 ng of these substrates were electroporated into 200,000 cells with 1 ug of pMAX-GFP (Lonza) using the Neon system (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... 1-10 million activated P14 cells were re-suspended in 20 μL primary cell nucleofection solution (Lonza), then mixed and incubated with the crRNA/Cas9 mix for 2 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The overlay medium contained either DMEM with 2% FBS and 1% sea-plaque agarose (Lonza, Walkersville, MD) in the case of in vitro samples or Opti-MEM with 2% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... After 1 h the medium was carefully replaces with supplemented hepatocyte maintenance medium (HMM) (#MP250, Lonza, Swizerland) until stimulations ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured at 1000-1500 cells/mm2 on 1% gelatin-coated dish in EGM-2 medium (Lonza) and used before passage 9 ...
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bioRxiv - Biophysics 2019Quote: ... Approximately 1 to 1.5 × 106 hPSCs were washed in DPBS and resuspended in P3 primary buffer (Lonza) with 1 μg gRNA ...
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bioRxiv - Microbiology 2021Quote: ... SuBK12-08 and Tb 1 Lu in Eagle’s Minimum Essential Medium (EMEM, Lonza Cat. No: 12-662F) with 1% of L-Glutamine (200 mM ...
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bioRxiv - Genomics 2022Quote: ... Following 6-TG treatment 5×106 HM-1 cells were transfected using a Nucleofector®-4D (Lonza) with the P3 Primary Cell X kit ...
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bioRxiv - Molecular Biology 2022Quote: ... 1·106 of HEK293 cells were resuspended in 100 μL of pre-warmed Nucleofector solution V (Lonza). The plasmid containing c17orf80 gRNA and Cas9 (4 μg ...
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bioRxiv - Bioengineering 2022Quote: RPE-1 cells were grown in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12, Lonza) supplemented with 10% Fetal Bovine Serum (GIBCO ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were split at a 1:2 ratio every 2 days by trypsinization (Lonza cat# CC-5012), followed by centrifugation at 200 x g for 3 min before resuspending and reseeding in EBM Basal Medium supplemented with the EGM Singlequots Supplement Pack ...
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bioRxiv - Bioengineering 2024Quote: ... 2.5×105-1×106 CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland, cat.: V4XP-3032) with complexed RNPs and electroporated using the Lonza 4D Nucleofector and 4D-Nucleofector X Unit (program DZ-100) ...
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bioRxiv - Cell Biology 2023Quote: The following hTERT RPE-1 cells were all tested free of mycoplasma contamination using Myco Alert (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 105 to 2 × 105 cells were collected and resuspended in 15 µL of nucleofection buffer (Lonza). For each reaction ...
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bioRxiv - Cancer Biology 2023Quote: 1 x 103 cells per dish were plated onto 35mm dishes in 0.4% NuSieve Agarose (Lonza #50081). Six replicates of each condition were plated ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with pcDNA3.1 encoding full-length mouse PD-1 or its mutant using nucleofection (Lonza). Transfected cells were sorted for uniform PD-1 staining and culture in medium with 0.4 cells and reporter Jurkat cells stably expressing WT or MT chimeric PD-1 (mhPD-1 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were centrifuged and the supernatant was aspirated and pellet was resuspended in the appropriate volume of P3 supplement buffer (20μl per condition by mixing 16.4μl P3 buffer and 3.6μl of supplement 1, Lonza, V4XP-3032). The cells were transferred into 16-well nucleovette strips (Lonza) ...
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bioRxiv - Microbiology 2023Quote: ... siRNAs (1 μg per ml) were nucleofected into ISE6 cells using the 4D-Nucleofector System (Lonza Bioscience). Tick cells were centrifuged at 100 x g for 10 minutes to pellet the cells ...
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bioRxiv - Genetics 2024Quote: ... RNP complexes together with the repair template were transfected into 1 million iPSCs with Amaxa nucleofector (Lonza, Human Stem Cell kit 2 ...
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bioRxiv - Neuroscience 2021Quote: ... LUHMES cells were electroporated and transfected with 2 μg of plasmid using the Amaxa™ Basic Primary Neurons Nucleofector™ Kit and protocol (Lonza, cat. # VPI-1003). Transfected cells were selected by flow-sorting for DAPI negative ...
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bioRxiv - Cell Biology 2019Quote: Nucleofection of HeLa cells was performed using a Lonza 4D-Nucleofector™ device and Amaxa™ SE Cell Line 4D-Nucleofector™ X Kit (Lonza - V4XC-1024), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The cells were cultured in a humidified chamber at 37 °C under 5% CO2 and were routinely tested for mycoplasma contamination using MycoAlert plus detection kit (Lonza, Basel, Switzerland; cat. LT07-701).
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bioRxiv - Genomics 2022Quote: ... 1.0µg/µl SpCas9-sgRNA-PGK-Venus construct and 1µg/µl donor construct were nucleofected into ∼3×106 Tir1 mESCs using the Amaxa™ 4D-Nucleofector and the P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza, Cat. V4XP-3024) following the manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: 1-1.5 x106 triple reporter TCR-/- Jurkat cells were mixed with 2μg of pLVM containing gene of interest and electroporated using pulse code CL-120 according to manufacturer’s protocol (Lonza #V4XC-1024, SE Cell line kit). After 48-72hrs ...
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bioRxiv - Cancer Biology 2024Quote: ... hTERT-HME1 (CRL-4010) were acquired from ATCC and cultured in Mammary Epithelial Cell Growth Medium Bullet Kit from Lonza (Bend, OR, Catalog #: CC-3150).
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bioRxiv - Cell Biology 2023Quote: ... BU3 NG iPSCs were pretreated with ROCK Inhibitor (Y-27632) for three hours prior to nucleofection using the Lonza P3 Primary Cell 4DNucleofector™ X Kit (Lonza, cat. no. V4XP-3024) and replated for puromycin resistance screening ...
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bioRxiv - Immunology 2021Quote: ... and the next day (day 1) sequentially seeding primary human lung microvascular endothelial cells (Lonza, CC-2527, P5) and primary human lung alveolar epithelial cells (Cell Biologics ...
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bioRxiv - Genomics 2019Quote: ... The DNA fragments of 150–200 bp length were gel-extracted on 3% NuSieve 3:1 Agarose (Lonza). Both samples were sequenced by GATC Biotech AG (Konstanz ...
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bioRxiv - Molecular Biology 2019Quote: ... a modified antisense or control oligonucleotide (1 μM) was transfected into 3 × 106 cells using Nucleofector technology (Lonza) 16–18 h before the assay ...
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bioRxiv - Biophysics 2019Quote: ... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ...
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bioRxiv - Genetics 2019Quote: ... Between 1×10^4 – 5×10^4 cells per condition were resuspended in 20μl of Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the Cas9 gRNA RNP complex ...