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Citations for Lonza :
2651 - 2700 of 2843 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines (and their derivatives) were confirmed to be mycoplasma-free using the MycoAlert mycoplasma detection kit (Lonza LT07- 218) at the beginning and upon completion of all experiments.
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bioRxiv - Cancer Biology 2023Quote: ... We periodically tested all the cell lines for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, cat. no. LT07-118). Culturing conditions were as follows ...
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bioRxiv - Immunology 2023Quote: ... Electroporation was performed with sgRNA/Cas9 RNP complexes using Alt-R Sp Cas9 Nuclease V3 (IDT) and using the 4D-Nucleofector with P3 Primary Cell 4D Nucleofector X Kit S (Lonza Bioscience).
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bioRxiv - Synthetic Biology 2024Quote: ... Then the supernatant was removed and cells were resuspended in 100 µl room-temperature Nucleofector® Kit V (Lonza, VCA-1003) solution ...
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bioRxiv - Cell Biology 2024Quote: ... All mouse iPSC lines screened negative for mycoplasma contamination every other month using a MycoAlert PLUS detection kit (Lonza, LT07-710).
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bioRxiv - Bioengineering 2024Quote: ... and recovered for at least 24 hours in Hepatocyte Culture MediaTM (HCM) including the complete HCM bullet kit (Lonza, #CC-3198) according to the supplier’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Cell lines in culture were screened biweekly for mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, 801 #LT07-703).
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bioRxiv - Cancer Biology 2024Quote: ... were purchased from Sigma and transfected into MCF-7 cells at a final concentration of 30 nM by electroporation using the SE Cell Line 4D-Nucleofector X kit (Lonza; EEID:SCR_023155). The cells were used for various assays 48 hours after siRNA transfection.
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bioRxiv - Bioengineering 2024Quote: ... All stem cell and organoid lines were routinely monitored for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza Cat#LT07-318).
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bioRxiv - Cancer Biology 2024Quote: ... Primary CD3+ T cells (1×106 cells/transfection) were transfected using a Lonza 4D nucleofector™ (Pulse code EH115) with a P3 nucleofection kit (Lonza) following manufacturer’s recommendations with the indicated DNA concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... K562 and NALM6 cells (2×105 cells/transfection) were transfected using a Lonza 4D nucleofector™ with a SF nucleofection kit (Lonza) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... brucei 13-90 cells were electroporated with 10 µg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (program X-001) (Lonza, Switzerland).
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bioRxiv - Biochemistry 2024Quote: ... 5.0 × 106 OSCs were mixed with 200 pmol of siRNAs in 20 μL of Solution SF of the Cell Line Nucleofector Kit SF (Lonza Bioscience) and electroporation was performed using a Nucleofector 96-well Shuttle device (Lonza Bioscience) ...
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bioRxiv - Neuroscience 2024Quote: ... and pCAG-Puro-Hs-HARE5 (2µg) were electroporated into 1×106 C3649 cells using the P3 Primary Cell 4D-Nucleofector kit (Lonza, V4XP-3024). The selection and genotyping process was the same as above ...
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bioRxiv - Neuroscience 2024Quote: ... and pCAG-Puro-Pt-HARE5 (2µg) were electroporated into 1×106 H9 cells using the P3 Primary Cell 4D-Nucleofector kit (Lonza, V4XP-3024). Following each electroporation ...
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bioRxiv - Neuroscience 2024Quote: All lines tested negative for mycoplasma contamination in this study were checked by the MycoAlert Mycoplasma Detection Kit (Lonza, LT07-518). One human ESC line WA09 (H9) ...
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bioRxiv - Synthetic Biology 2024Quote: ... K562 cells were electroporated with 800ng of Bxb1 pDNAusing the SF cell line 96-well Nucleofector kit (Lonza, Catalog#V4SC-2960), using manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... for all imaging experiments involving exogenous expression we used the Lonza Amaxa II Nucleofector System with Cell Line Nucleofector Kit V reagent (Lonza VCA-1003). Briefly ...
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bioRxiv - Cell Biology 2020Quote: 1 million Jurkat cells were electroporated with the 2.5 μg of sgRNA plasmid and GFP control plasmid at a 10:1 ratio using an Amaxa Cell Line Nucleofector Kit V and an Amaxa™ Nucleofector™ II (Lonza). HeLa ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs were transfected into 3T3-F442A preadipocytes or OP9 preadipocytes via electroporation using the Amaxa Cell Line Nucleofector Kit V (Lonza, Cologne, Germany). Afterwards cells were grown two days in DMEM ...
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bioRxiv - Immunology 2021Quote: ... 1.5×106 cells multiplied by the number of mice to be injected were transfected with the pIL-10 vector (1×106 cells/1.5 μg pIL-10 DNA per cuvette) using the Mouse T Cell Nucleofector Kit (Lonza, No: VPA-1006) with Nucleofector II Device (program X-100) ...
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bioRxiv - Developmental Biology 2021Quote: Human isogenic ESCs containing different lengths of CAG repeats in the HTT Exon1 locus (HD-RUES2 6) were nucleofected using the Cell Line Nucelofector II (Kit L from Lonza, Walkersville, MD) by applying the B-016 program ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Electroporation of K562 cells was performed using a SF Cell Line 4D-Nucleofector kit and 4D-X Nucleofector using pulse code FF-120 (Lonza, Basel, Switzerland), per the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and CD31+Rspo3+ cells were centrifuged at 500 g for 5 min and resuspended in 500 μl endothelial cell media: EGM-2 MV Bullet Kit (Lonza, cc-3202) supplemented with 50 ng/mL VEGF-C (R&D ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The siRNA knockdown was performed by electroporation using the Amaxa nucleofector I in combination with the Amaxa Cell Line Nucleofector Kit V (Lonza, VCA-1003). SiRNA constructs with the siRNA cloned in the pLKO.1-Puro vector were obtained from the MISSION shRNA library (Sigma product SHGLY) ...
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bioRxiv - Microbiology 2020Quote: ... using the AMAXA Nucleofector 4D system (U-033 setting) and the P3 primary cell 4D-nucleofector X kit with the 16-well nucleocuvette strip (Lonza, V4XP-3032). Clones were obtained by FACS using the FACS Aria II sorter at the Stanford Shared FACS Facility for the brightest red parasites and single cloning the TdTomato+ enriched population by limiting dilution into 96-well plates.
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bioRxiv - Bioengineering 2019Quote: Nucleofection of the HDR template and CRISPR-Cas9 vectors was performed with SF Cell Line 4D-Nucleofection X Kit L (Lonza, V4XC-2024). Before nucleofection hybridoma cells were assessed for viability and centrifuged (90g ...
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bioRxiv - Neuroscience 2019Quote: ... were provided under a MTA and maintained in Clonetics® endothelial cell growth medium-2 MV Bullet Kit (Cat# CC-3162 Lonza, UK) containing the endothelial basal medium ...
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bioRxiv - Neuroscience 2019Quote: ... C57BL/6J: L315 control construct or L315 Nrxn triple knockdown construct) just before plating using AMAXA Nucleofector kit (Lonza, Cat #VPG-1001) and plated at 2500/cm2 on micro islands of mouse (CD-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 µg of PX459 gRNA vector (5’-GACTCCAGTCTTTCTAGAAGA-3’) were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-100) using program A-30 in the XRep ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 μg of the donor ssDNA and 3 μg of a puromycin resistance plasmid (pCAG-puroR) using the Mouse ES Cell Nucleofector™ Kit (Lonza, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines identities were validated by STR profiling and tested negative for mycoplasma with MycoAlert Mycoplasma Detection Kit (Lonza, Cat#LT07-418) prior to experimental use.
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells (grown in 6-well dishes) were subjected to nucleofection with Amaxa® Cell Line Nucleofector® kit V (VCA-1003, Lonza), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 bulk or stable populations overexpressing Nluc-CD63 or Nluc-CD9 constructs were obtained using an electroporation-based transfection protocol optimized for this cell line (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Briefly ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... complex with a ratio of 4.5 to 1 between sgRNA and Cas9 was delivered following the protocol of the SE Cell Line 4D-NucleofectorTM X Kit (Lonza, V4XC-1012), using the nucleofection program DS-130 on the Lonza 4D X unit ...
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bioRxiv - Cell Biology 2021Quote: ... subjected to red blood cell lysis with ACK lysis buffer and electroporated with P3 Primary Cell 4D-NucleaofactorTM X -kit (V4XP-3032, Lonza, Basel, Switzerland), as previously described (Freund et al. ...
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bioRxiv - Cell Biology 2021Quote: ... We transfected the KCNQ1 constructs and the empty vector into our established mouse pancreatic β cells (referred to as SJ β cells (Jia et al., 2015)) using AmaxaTM Cell Line NucleofectorTM Kit V and AmaxaTM NucleofectorTM 2b device (Lonza, Switzerland). The glucose-sensitive mouse β-cell line SJ-β was cultured and an insulin-secretion assay performed as reported earlier (Jia et al. ...
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bioRxiv - Systems Biology 2021Quote: ... freshly isolated neurons (5 million cells per nucleofection reaction) were transfected using the 4D-Nucleofector™ X Unit and the P3 Primary Cell 4D Nucleofector X kit (Lonza, Switzerland), as indicated ...
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bioRxiv - Cell Biology 2020Quote: ... These plasmids were transfected into NHDFs and 7SPs by electroporation using the Amaxa nucleofector kit and nucleofector 2b (Lonza, Walkersvill, MD, USA). Two days later ...
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bioRxiv - Cancer Biology 2021Quote: ... All human cell lines were authenticated by Short Tandem Repeat analysis and tested for Mycoplasma contamination with a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
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bioRxiv - Cancer Biology 2021Quote: ... AsPC-1 and CAPAN-1 pellets were suspended in Lonza transfection reagent (SE Cell Line 4D-Nucleofector™ X Kit L, Lonza, Germany) when the Mirus transfection reagent was finished ...
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bioRxiv - Cell Biology 2021Quote: ... 500μg of gRNA and 500μg of donor plasmids were electroporated in 1×106 mESC using P3 Primary Cell 96-well Nucleofector™ Kit (Lonza, PBP3-22500) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Genomics 2022Quote: ... 5 million cells were transfected with 10ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit T (Lonza), program X-001 ...
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bioRxiv - Molecular Biology 2022Quote: ... Electroporation of 5×106 HL-60 cells was performed with AIO-GFP HAX1 LCRC1 and AIO-GFP HAX1 LCRC2 using CLB-Transfection™ Kit (Lonza, Austria) and CLB-Transfection™ System (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines were cultures for no more than 30 passages and routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza, Slough, UK).
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Neuroscience 2022Quote: ... All cell lines were verified to be mycoplasma free by first culturing the cells in antibiotic-free media and then subjected to a mycoplasma tested using MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza, UK).
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bioRxiv - Biophysics 2022Quote: ... 106 U2OS cells were transfected with 1 μg of px330 plasmid and 1 μg of the HDR donor plasmid using the Nucleofector 2b device and cell line nucleofector kit V (Lonza, VCA-1003) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 1uM siRNA was electroporated into cells using the Amaxa Nucleofector II device and Cell Line Nucleofector Kit V (Lonza Bioscience, VCA-1003). B16-F10 cells were electroporated with program P-020 ...