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Citations for Lonza :
2351 - 2400 of 2843 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X S kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Enriched naïve PbTII GFP+ cells were resuspended in 20μl of P3 Primary buffer (P3 Primary Cell 4D-Nucleofector X Kit, Lonza) and mixed with sgRNA/Cas9 RNP ...
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bioRxiv - Neuroscience 2022Quote: ... and sgRNAs against LRRK2 (crRNAs ordered from IDT) were introduced into iPSCs via nucleofection (Lonza P3 kit # V4XP-3032) as described before(75) ...
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bioRxiv - Molecular Biology 2022Quote: ... 8×107 promastigotes were transfected with 1 µg (≈ 1,68.1011 molecules) of the linearized barcoding library using the Basic Parasite Nucleofector™ kit 1 (Lonza) with the U-033 program ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 x 105 cells were transfected with the pU6-Sp-pegRNA-HEK3-insCTT plasmid (1,000 ng) using the SF Cell Line 96-well Nucleofector Kit (Lonza). Program code was FF-120.
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bioRxiv - Cancer Biology 2023Quote: ... hTERT ipn02.3 2λ cells were transfected by electroporation using the Amaxa Basic Nucleofector Kit for Primary Mammalian Fibroblasts (Lonza) and the program U-023 ...
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bioRxiv - Cancer Biology 2023Quote: ... Regular testing for mycoplasma contamination was conducted every 3-4 weeks using the MycoAlert PLUS mycoplasma detection kit (Lonza).
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bioRxiv - Bioengineering 2023Quote: ... and 40 pmol of single-stranded oligonucleotide donor DNA (ssODN) were nucleofected to 0.25 × 106 HEK293T cells using the SF Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of CM-130 ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines used in this study were regularly screened for Mycoplasma contamination with the MycoAlert Detection Kit (Lonza).
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bioRxiv - Cell Biology 2023Quote: ... and mN designates the 2’-O-methylribonucleotides) was transfected into HAP1 cells (1.0 × 106 cells) by Nucleofector Kit V (Lonza) with a Nucleofector device (Lonza ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were pulsed with protocol EO115 using the P3 Primary Cell 4D-Nucleofector Kit and 4D-Nucleofector System (Lonza). Cells were recovered immediately with warm media for 6 hours before transduction with CAR ...
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bioRxiv - Neuroscience 2023Quote: ... All CRISPR reagents were electroporated into iPSCs using the P3 Primary Cell 4D Nucleofector™ X Kit S (Lonza), as previously described.(25 ...
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bioRxiv - Bioengineering 2023Quote: ... with two phosphothiorate linkages on each end were mixed with 82 µL Nucleofector Solution V and 18 µL of Supplement Solution 1 according to manufacturer’s recommendations for the Cell Line Nucleofector Kit V (Lonza). 5×105 U2OS.EGFP parent cells were resuspended in the above mixture and nucleofected with a Lonza Nucleofector 2b Device (Kit V ...
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bioRxiv - Synthetic Biology 2024Quote: ... K562 cells were electroporated with pDNA using the SF cell line 96-well Nucleofector kit (Lonza, Catalog#V4SC-2960) or SF Cell Line 384-well Nucleofector Kit (Lonza ...
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bioRxiv - Neuroscience 2024Quote: Nucleofections were done on cortical neurons using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, LZ-V4XP-3024), on the day of preparation and dissociation (DIV 0) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell culture media supernatant was tested for mycoplasma contamination every 4lJweeks using the MycoAlert PLUS mycoplasma detection kit (Lonza) and all tests were negative throughout the experiments.
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bioRxiv - Developmental Biology 2024Quote: ... Mycoplasma testing was carried out by DAPI staining of fixed cultures and using the mycoalert mycoplasma detection kit (Lonza). We confirm that the cell lines used in this study were free of mycoplasma and aerobic bacteria and fungi.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were confirmed as negative for mycoplasma contamination by using a Lonza Mycoplasma Detection Kit (Lonza Bioscience, Durham, NC) before injection ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were verified as negative for mycoplasma by testing with the MycoAlert Mycoplasma Detection Assay kit (Lonza, LT07–318) per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were routinely tested for mycoplasma contamination using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza LT07-710).
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... Routine mycoplasma testing was performed every 6 months to ensure cell quality using the MycoAlert™ Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...
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bioRxiv - Cell Biology 2020Quote: ... Modified MTEC culture media is comprised of small airway basal media (SABM) with selected components from SAGM bullet kit (Lonza) including Insulin ...
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bioRxiv - Cell Biology 2020Quote: U-2 OS cells stably expressing spdCas9-GFP were nuclofected with Amaxa® Cell Line Nucleofector® Kit V (Lonza). Cells were detached with 0.05% trypsin/EDTA and spun down by centrifugation at 1000rpm for 5 min ...
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bioRxiv - Immunology 2021Quote: ... WT RAW264.7 cells were subjected to two rounds of nucleofection with Cas9-RNPs using the SF Cell Line 4D-Nucleofector X Kit S (Lonza) and the 4D Nucleofector apparatus precisely as specified by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... Constructs containing validated gRNAs were electroporated together with a vector expressing puromycin into hESCs using the P3 Primary Cell 4D-Nucleofector X kit (Lonza) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the RNP complexes were added to 1×105 cells in 40 μl of P3 nucleofection buffer from the 4D-Nucleofector X kit (Lonza). Half of the mixture was loaded to each well of a 16-well nucleofection cassette and nucleofected using the using E0-100 program with the 4D-Nucleofector Lonza Amaxa ...
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bioRxiv - Microbiology 2021Quote: ... were electroporated with 20 ug of guide plasmid and 60 ug of linearized repair plasmid using an Amaxa 4D electroporator and P3 Primary cell 4D Nucleofector X Kit L (Lonza) using programme FP158 as described (Collins et al. ...
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bioRxiv - Immunology 2022Quote: ... a total of 1.6 x 108 cells were transfected with 2 μM of the corresponding ASO using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza) and the EO-115 program ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 90 μL of nucleofection solution (16.2 μL of Supplement solution mixed with 73.8 μL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza) was mixed thoroughly with the cell pellet ...
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bioRxiv - Immunology 2021Quote: Control or transfected Lymphocytes (with Flag-wt-TDP-43 or Flag-NLS-mut-TDP-43 plasmid (1 μg) using nucleofection kit (Lonza)) were placed on coverslips (2 x 106 cells in sterile glass coverslips-Ø 12 mm ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Cancer Biology 2019Quote: ... All cells were authenticated by short tandem repeat analysis and confirmed negative for Mycoplasma infection with the MycoAlert Mycoplasma Detection Kit (Lonza). HCT-116 and A549 cells were cultured in DMEM ...
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bioRxiv - Cancer Biology 2020Quote: Bioluminescent detection of cellular ATP as a measure of cell viability was undertaken using ViaLight® Plus Kit (Lonza Inc.) reagents ...
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bioRxiv - Bioengineering 2019Quote: ... and grown in bronchial epithelial growth medium (BEGM) supplemented with SingleQuot additives from Lonza (BEGM Bullet Kit, reference CC-3170) and 50 μg/mL G-418 at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were obtained from the Berkeley Cell Culture Facility and were verified mycoplasma free with MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Neuroscience 2019Quote: ... the neurons were resuspended in DMEM/F12 and electroporated using a P3 Primary Cell 4D-Nucleofector X Kit L (cat. no. V4XP-3012, Lonza) in accordance with the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Each chamber was connected to a reservoir that was pre-filled with 15 mL of warm serum-free EGM-2 (endothelial cell growth medium-2 without serum added from the kit, Lonza). ECFCs were harvested and resuspended in serum-free EGM-2 and added to the reservoirs to reach an overall cell density of 125,000 cells/mL ...
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bioRxiv - Genomics 2020Quote: ... 5 million cells were transfected with 5 μg of DNA FAIRE-STARR library plasmid using the Amaxa Nucleofector kit V (Lonza). For each condition ...
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bioRxiv - Systems Biology 2021Quote: ... and 2×106 iPSCs were mixed with pX459-sgRNA vector and ssDNA repair donor template and electroporated with Lonza 4D-Nucleofector system using the P3 Primary Cell Nucleofector Kit (Lonza). The iPSCs were then recovered in mTeSR1 medium for 48 hours before adding puromycin for 3-day selection ...
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... 200,000 cells were electroporated with 1000 ng adRNA plasmid or 48 pmol of IVT RNA using the Amaxa SF cell Line 4D-Nucleofector X kit (Lonza) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... The efficacy of siRNA targeting Kdm6a was assessed by electroporation of neurons using a 4D-Nucleofector X Unit and the corresponding P3 Primary Cell nucleofection kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Their identity was verified by using STR profiling (Eurofins) and lack of Mycoplasma infection was confirmed by Mycoalert Mycoplasma Detection Kit (Lonza). Cell lines were cultured in Dulbecco’s modified eagle’s medium (DMEM):F12-HAM (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The RNP complex and 1.5 μg of a linearized homology-directed repair template plasmid were transfected into 2×105 – 5×105 nocodazole-arrested mitotic HeLa A12 cells using a Nucleofector and the associated Cell Line Kit R (Lonza) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... All of the cell lines have been periodically subjected to re-confirmation by Short Tandem Repeat (STR) profiling by ATCC and mycoplasma testing by MycoAlertTM PLUS mycoplasma detection Kit (Lonza). A375 ...
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bioRxiv - Microbiology 2021Quote: ... Raw264.7 Ifit2 CRISPR cells were generated by electroporating low passage Raw264.7 cells with Ifit2 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...
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bioRxiv - Neuroscience 2020Quote: iPSNs were transfected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza). POM121 ...
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bioRxiv - Microbiology 2021Quote: ... Both resuspensions were mixed in a 100 μl cuvette (P3 Primary cells 4D-Nucleofector X kit L, V4XP-3024, Lonza). The programme FI-158 was used for electroporation ...