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Citations for Lonza :
151 - 167 of 167 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... confluent 293T-ΔNC cells were treated with a solution of 0.5 g/L trypsin and 0.2 g/L ethylene diaminetetraacetic acid (EDTA) without calcium or magnesium (Lonza; 17-161E) at 37°C to detach and dissociate the cells ...
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bioRxiv - Cell Biology 2024Quote: ... Differentiation medium consisted of 50% DMEM and 50% BEBM supplemented with BEGM SingleQuots (except amphotericin B, triiodothyronine, and retinoic acid; CC-3171 and CC-4175, Lonza). Medium was supplemented with 100 nM all-trans retinoic acid (R2625 ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 11 mM glucose and 25 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) pH 7.4 (Lonza, Basel, Switzerland). The apical (AP ...
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bioRxiv - Bioengineering 2021Quote: ... we nucleofected HEK293FT-RfxCas13d cells with 225 μmol of synthetic crRNAs in 20μl reaction of SF Cell Line Nucleofector Solution (Lonza V4XC-2032) using the Lonza Nucleofector 4D (program CM-130) ...
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bioRxiv - Cancer Biology 2021Quote: ... in a total volume of 100μL/ reaction and electroporated using an Amaxa TM Basic Nucleofector TM Kit for Primary Mammalian Epithelial cells (Lonza, # VPI-1005) and the NucleofectorTM 2b Device (Lonza ...
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bioRxiv - Immunology 2020Quote: ... Six micrograms of plasmid DNA encoding the cDNA of the desired proteins was used for a single transfection reaction using program T-019 on the Amaxa Nucleofector II (Lonza, MD).
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bioRxiv - Neuroscience 2023Quote: ... Approximately 2 million cells were nucleofected in a large cuvette per reaction using Lonza’s P3 primary cell 4D-nucleofector kit (V4XP-3024, Lonza, Basel, Switzerland). The cells were allowed to recover from the transfection for 3 days while inducing MNX+ motor neuronal cell fate ...
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bioRxiv - Microbiology 2021Quote: ... electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001).
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bioRxiv - Bioengineering 2024Quote: ... with L-ascorbic acid-2-phosphate (AA2P) (5mg/ml stock, 10 µl/ml), and β-glycerolphosphate (BGP) (20 mM stock, 20 µl/ml) (Lonza). The cell culture medium was changed every 3 days ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in mEC medium (0.1% bovine brain extract (BBE)/10% FBS/1%penicillin-streptomycin/1% Non-Essential Amino Acid (NEAA)/Dulbecco Modified Eagle Medium (DMEM) (DMEM Cat#: 41966-029, Gibco; BBE Cat#: CC-4098, Lonza BioScience). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO2 (HDLECs and HUVECs ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293S GnTI- cells were grown in DMEM with 10% FBS and 1 X nonessential amino acids (NEAAs) from Lonza (Basel, Switzerland). Cells were grown at 37 °C ...
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bioRxiv - Systems Biology 2021Quote: ... freshly isolated neurons (5 million cells per nucleofection reaction) were transfected using the 4D-Nucleofector™ X Unit and the P3 Primary Cell 4D Nucleofector X kit (Lonza, Switzerland), as indicated ...
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bioRxiv - Microbiology 2023Quote: ... 2□μl of each RNP complex were then mixed with the cell suspension and transferred into a 16-well reaction cuvette of the 4D-Nucleofector System (Lonza #V4XP-3032). The CD4+ T cells and CD34+ cells were electroplated using the programs EH-100 and DZ-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 20uL prepared electroporation buffer (16.4uL SE or P3 + 3.6uL supplement buffer per reaction) provided by the Lonza 4D-Nucleofector X kit (SE, Fisher Scientific, #NC0828118; or P3, Lonza; #V4XP-3032.) 20uL of the cell solution was then transferred to the 5uL RNP solution and gently mixed by pipetting ...
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bioRxiv - Bioengineering 2024Quote: ... 50 pmol of Cas9 was mixed with 100 pmol of gRNA for every 20 µL of reaction volume (equivalent to volume required for one Lonza nucleofection cuvette). After mixing of the Cas9 protein with the gRNA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... droplets in 6 well plates and treated with retinoic acid for 4 days followed by hepatocyte growth media (Hepatocyte Culture Medium BulletKit (Lonza, CC-3198) supplemented with 10 ng/mL hepatocyte growth factor (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: Positive control of reactions was performed by inducing the differentiation of hDPSCs using an osteogenic induction medium (Differentiation Basal Medium-Osteogenic, Lonza, Walkersville, MD, USA) 37°C with 5% CO2 tension and were maintained for 21 days ...